Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2473 and Pf1N1B4_2474 overlap by 4 nucleotides Pf1N1B4_2474 and Pf1N1B4_2475 are separated by 291 nucleotides Pf1N1B4_2475 and Pf1N1B4_2476 overlap by 4 nucleotides
Pf1N1B4_2473 - Transmembrane sensor, at 2,805,111 to 2,806,085
_2473
Pf1N1B4_2474 - RNA polymerase sigma-70 factor, ECF subfamily, at 2,806,082 to 2,806,558
_2474
Pf1N1B4_2475 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 2,806,850 to 2,807,557
_2475
Pf1N1B4_2476 - Predicted signal-transduction protein containing cAMP-binding and CBS domains, at 2,807,554 to 2,809,494
_2476
Position (kb)
2806
2807 Strain fitness (log2 ratio)
-1
0
1
2 at 2805.758 kb on - strand, within Pf1N1B4_2473 at 2806.098 kb on + strand at 2806.098 kb on + strand at 2806.098 kb on + strand at 2806.099 kb on - strand at 2806.099 kb on - strand at 2806.099 kb on - strand at 2806.099 kb on - strand at 2806.099 kb on - strand at 2806.110 kb on + strand at 2806.110 kb on + strand at 2806.110 kb on + strand at 2806.110 kb on + strand at 2806.110 kb on + strand at 2806.110 kb on + strand at 2806.110 kb on + strand at 2806.111 kb on - strand at 2806.111 kb on - strand at 2806.111 kb on - strand at 2806.111 kb on - strand at 2806.111 kb on - strand at 2806.137 kb on + strand, within Pf1N1B4_2474 at 2806.138 kb on - strand, within Pf1N1B4_2474 at 2806.212 kb on + strand, within Pf1N1B4_2474 at 2806.212 kb on + strand, within Pf1N1B4_2474 at 2806.213 kb on - strand, within Pf1N1B4_2474 at 2806.213 kb on - strand, within Pf1N1B4_2474 at 2806.213 kb on - strand, within Pf1N1B4_2474 at 2806.213 kb on - strand, within Pf1N1B4_2474 at 2806.347 kb on + strand, within Pf1N1B4_2474 at 2806.347 kb on + strand, within Pf1N1B4_2474 at 2806.348 kb on - strand, within Pf1N1B4_2474 at 2806.348 kb on - strand, within Pf1N1B4_2474 at 2806.348 kb on - strand, within Pf1N1B4_2474 at 2806.348 kb on - strand, within Pf1N1B4_2474 at 2806.377 kb on + strand, within Pf1N1B4_2474 at 2806.378 kb on - strand, within Pf1N1B4_2474 at 2806.378 kb on - strand, within Pf1N1B4_2474 at 2806.532 kb on + strand at 2806.532 kb on + strand at 2806.533 kb on - strand at 2806.533 kb on - strand at 2806.554 kb on + strand at 2806.669 kb on + strand at 2806.669 kb on + strand at 2806.670 kb on - strand at 2806.851 kb on + strand at 2806.851 kb on + strand at 2806.851 kb on + strand at 2806.915 kb on + strand at 2806.916 kb on - strand at 2807.121 kb on + strand, within Pf1N1B4_2475 at 2807.122 kb on - strand, within Pf1N1B4_2475 at 2807.395 kb on + strand, within Pf1N1B4_2475 at 2807.429 kb on + strand, within Pf1N1B4_2475 at 2807.557 kb on + strand at 2807.557 kb on + strand at 2807.557 kb on + strand at 2807.558 kb on - strand at 2807.558 kb on - strand at 2807.558 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 2,805,758 - Pf1N1B4_2473 0.66 -0.5 2,806,098 + +1.5 2,806,098 + +0.9 2,806,098 + -0.3 2,806,099 - -0.3 2,806,099 - +0.0 2,806,099 - +0.1 2,806,099 - +1.0 2,806,099 - +0.4 2,806,110 + -0.7 2,806,110 + +0.3 2,806,110 + +0.0 2,806,110 + -0.3 2,806,110 + +2.3 2,806,110 + +2.3 2,806,110 + -0.5 2,806,111 - +0.1 2,806,111 - -1.0 2,806,111 - -1.2 2,806,111 - +0.1 2,806,111 - -0.7 2,806,137 + Pf1N1B4_2474 0.12 +0.3 2,806,138 - Pf1N1B4_2474 0.12 +2.3 2,806,212 + Pf1N1B4_2474 0.27 -1.0 2,806,212 + Pf1N1B4_2474 0.27 -1.3 2,806,213 - Pf1N1B4_2474 0.27 -1.3 2,806,213 - Pf1N1B4_2474 0.27 -0.7 2,806,213 - Pf1N1B4_2474 0.27 -1.5 2,806,213 - Pf1N1B4_2474 0.27 +1.0 2,806,347 + Pf1N1B4_2474 0.56 +0.0 2,806,347 + Pf1N1B4_2474 0.56 +0.9 2,806,348 - Pf1N1B4_2474 0.56 -0.0 2,806,348 - Pf1N1B4_2474 0.56 -0.4 2,806,348 - Pf1N1B4_2474 0.56 +0.3 2,806,348 - Pf1N1B4_2474 0.56 -0.3 2,806,377 + Pf1N1B4_2474 0.62 -0.2 2,806,378 - Pf1N1B4_2474 0.62 -1.5 2,806,378 - Pf1N1B4_2474 0.62 -0.7 2,806,532 + -0.6 2,806,532 + +0.5 2,806,533 - -0.1 2,806,533 - +1.0 2,806,554 + +0.4 2,806,669 + +0.1 2,806,669 + -0.8 2,806,670 - +2.3 2,806,851 + +0.6 2,806,851 + -0.5 2,806,851 + +0.2 2,806,915 + -1.0 2,806,916 - +0.9 2,807,121 + Pf1N1B4_2475 0.38 +0.8 2,807,122 - Pf1N1B4_2475 0.38 +0.3 2,807,395 + Pf1N1B4_2475 0.77 -0.9 2,807,429 + Pf1N1B4_2475 0.82 -0.7 2,807,557 + +0.3 2,807,557 + -0.1 2,807,557 + -0.3 2,807,558 - +0.8 2,807,558 - +0.7 2,807,558 - +1.0
Or see this region's nucleotide sequence