Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_242 and Pf1N1B4_243 are separated by 1 nucleotides Pf1N1B4_243 and Pf1N1B4_244 overlap by 105 nucleotides Pf1N1B4_244 and Pf1N1B4_245 are separated by 219 nucleotides Pf1N1B4_245 and Pf1N1B4_246 are separated by 1 nucleotides Pf1N1B4_246 and Pf1N1B4_247 overlap by 4 nucleotides
Pf1N1B4_242 - Glutathione-regulated potassium-efflux system protein KefB, at 299,937 to 301,649
_242
Pf1N1B4_243 - Ammonium transporter, at 301,651 to 302,925
_243
Pf1N1B4_244 - hypothetical protein, at 302,821 to 302,946
_244
Pf1N1B4_245 - Probable transmembrane protein, at 303,166 to 303,480
_245
Pf1N1B4_246 - Probable transmembrane protein, at 303,482 to 303,871
_246
Pf1N1B4_247 - FIG00956101: hypothetical protein, at 303,868 to 304,206
_247
Position (kb) 301
302
303 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 300.677 kb on - strand, within Pf1N1B4_242 at 300.677 kb on - strand, within Pf1N1B4_242 at 301.207 kb on + strand, within Pf1N1B4_242 at 301.207 kb on + strand, within Pf1N1B4_242 at 301.207 kb on + strand, within Pf1N1B4_242 at 301.208 kb on - strand, within Pf1N1B4_242 at 301.218 kb on + strand, within Pf1N1B4_242 at 301.218 kb on + strand, within Pf1N1B4_242 at 301.218 kb on + strand, within Pf1N1B4_242 at 301.219 kb on - strand, within Pf1N1B4_242 at 301.342 kb on + strand, within Pf1N1B4_242 at 301.384 kb on + strand, within Pf1N1B4_242 at 301.621 kb on - strand at 301.703 kb on + strand at 301.704 kb on - strand at 301.704 kb on - strand at 301.704 kb on - strand at 302.188 kb on + strand, within Pf1N1B4_243 at 302.456 kb on + strand, within Pf1N1B4_243 at 302.456 kb on + strand, within Pf1N1B4_243 at 302.456 kb on + strand, within Pf1N1B4_243 at 302.457 kb on - strand, within Pf1N1B4_243 at 302.457 kb on - strand, within Pf1N1B4_243 at 302.588 kb on + strand, within Pf1N1B4_243 at 302.656 kb on + strand, within Pf1N1B4_243 at 302.656 kb on + strand, within Pf1N1B4_243 at 302.656 kb on + strand at 302.656 kb on + strand, within Pf1N1B4_243 at 302.657 kb on - strand, within Pf1N1B4_243 at 302.657 kb on - strand, within Pf1N1B4_243 at 302.657 kb on - strand at 302.658 kb on + strand, within Pf1N1B4_243 at 302.658 kb on + strand, within Pf1N1B4_243 at 302.658 kb on + strand, within Pf1N1B4_243 at 302.659 kb on - strand, within Pf1N1B4_243 at 302.659 kb on - strand, within Pf1N1B4_243 at 302.666 kb on + strand, within Pf1N1B4_243 at 302.666 kb on + strand, within Pf1N1B4_243 at 302.667 kb on - strand, within Pf1N1B4_243 at 302.820 kb on + strand at 302.820 kb on + strand at 302.820 kb on + strand at 302.821 kb on - strand at 303.008 kb on + strand at 303.009 kb on - strand at 303.079 kb on + strand at 303.080 kb on - strand at 303.104 kb on + strand at 303.105 kb on - strand at 303.128 kb on + strand at 303.128 kb on + strand at 303.129 kb on - strand at 303.160 kb on + strand at 303.397 kb on + strand, within Pf1N1B4_245 at 303.397 kb on + strand, within Pf1N1B4_245 at 303.482 kb on - strand at 303.482 kb on - strand at 303.716 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.752 kb on + strand, within Pf1N1B4_246 at 303.753 kb on - strand, within Pf1N1B4_246
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 300,677 - Pf1N1B4_242 0.43 -1.2 300,677 - Pf1N1B4_242 0.43 +1.0 301,207 + Pf1N1B4_242 0.74 -0.8 301,207 + Pf1N1B4_242 0.74 -0.6 301,207 + Pf1N1B4_242 0.74 +0.8 301,208 - Pf1N1B4_242 0.74 +1.6 301,218 + Pf1N1B4_242 0.75 -0.2 301,218 + Pf1N1B4_242 0.75 -0.2 301,218 + Pf1N1B4_242 0.75 +2.1 301,219 - Pf1N1B4_242 0.75 -0.8 301,342 + Pf1N1B4_242 0.82 -1.5 301,384 + Pf1N1B4_242 0.84 -1.2 301,621 - -0.0 301,703 + +1.3 301,704 - +1.2 301,704 - +0.2 301,704 - +0.4 302,188 + Pf1N1B4_243 0.42 +1.2 302,456 + Pf1N1B4_243 0.63 +0.8 302,456 + Pf1N1B4_243 0.63 -0.9 302,456 + Pf1N1B4_243 0.63 -2.9 302,457 - Pf1N1B4_243 0.63 +1.5 302,457 - Pf1N1B4_243 0.63 -0.2 302,588 + Pf1N1B4_243 0.73 -0.4 302,656 + Pf1N1B4_243 0.79 -0.8 302,656 + Pf1N1B4_243 0.79 -0.1 302,656 + -0.2 302,656 + Pf1N1B4_243 0.79 +0.2 302,657 - Pf1N1B4_243 0.79 -0.5 302,657 - Pf1N1B4_243 0.79 -0.9 302,657 - -0.2 302,658 + Pf1N1B4_243 0.79 -0.2 302,658 + Pf1N1B4_243 0.79 -0.8 302,658 + Pf1N1B4_243 0.79 -1.1 302,659 - Pf1N1B4_243 0.79 -0.2 302,659 - Pf1N1B4_243 0.79 +0.8 302,666 + Pf1N1B4_243 0.80 -1.1 302,666 + Pf1N1B4_243 0.80 -0.7 302,667 - Pf1N1B4_243 0.80 +0.8 302,820 + +0.2 302,820 + +0.2 302,820 + +0.2 302,821 - +0.2 303,008 + -0.2 303,009 - -0.4 303,079 + -0.2 303,080 - -0.4 303,104 + -0.2 303,105 - +1.0 303,128 + +0.8 303,128 + -0.8 303,129 - -1.0 303,160 + +1.4 303,397 + Pf1N1B4_245 0.73 -2.2 303,397 + Pf1N1B4_245 0.73 +0.8 303,482 - -1.5 303,482 - +0.8 303,716 + Pf1N1B4_246 0.60 +0.8 303,752 + Pf1N1B4_246 0.69 +0.8 303,752 + Pf1N1B4_246 0.69 -0.5 303,752 + Pf1N1B4_246 0.69 +1.4 303,752 + Pf1N1B4_246 0.69 +1.4 303,752 + Pf1N1B4_246 0.69 +0.2 303,752 + Pf1N1B4_246 0.69 +0.8 303,752 + Pf1N1B4_246 0.69 +0.2 303,752 + Pf1N1B4_246 0.69 -1.5 303,752 + Pf1N1B4_246 0.69 -0.8 303,752 + Pf1N1B4_246 0.69 -0.5 303,753 - Pf1N1B4_246 0.69 +2.0
Or see this region's nucleotide sequence