Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2197 and Pf1N1B4_2198 are separated by 21 nucleotides Pf1N1B4_2198 and Pf1N1B4_2199 are separated by 39 nucleotides Pf1N1B4_2199 and Pf1N1B4_2200 are separated by 15 nucleotides
Pf1N1B4_2197 - FIG00957574: hypothetical protein, at 2,509,109 to 2,509,834
_2197
Pf1N1B4_2198 - Lipoprotein YcfM, part of a salvage pathway of unknown substrate, at 2,509,856 to 2,510,443
_2198
Pf1N1B4_2199 - FIG00953537: hypothetical protein, at 2,510,483 to 2,510,854
_2199
Pf1N1B4_2200 - TPR domain protein, at 2,510,870 to 2,512,264
_2200
Position (kb)
2510
2511 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2509.483 kb on - strand, within Pf1N1B4_2197 at 2509.483 kb on - strand, within Pf1N1B4_2197 at 2509.530 kb on + strand, within Pf1N1B4_2197 at 2509.530 kb on + strand, within Pf1N1B4_2197 at 2509.530 kb on + strand, within Pf1N1B4_2197 at 2509.530 kb on + strand, within Pf1N1B4_2197 at 2509.531 kb on - strand, within Pf1N1B4_2197 at 2509.531 kb on - strand, within Pf1N1B4_2197 at 2509.531 kb on - strand, within Pf1N1B4_2197 at 2509.531 kb on - strand at 2509.531 kb on - strand, within Pf1N1B4_2197 at 2509.531 kb on - strand, within Pf1N1B4_2197 at 2509.531 kb on - strand, within Pf1N1B4_2197 at 2509.531 kb on - strand at 2509.557 kb on + strand, within Pf1N1B4_2197 at 2509.558 kb on - strand, within Pf1N1B4_2197 at 2509.558 kb on - strand, within Pf1N1B4_2197 at 2509.627 kb on + strand, within Pf1N1B4_2197 at 2509.627 kb on + strand, within Pf1N1B4_2197 at 2509.628 kb on - strand at 2509.628 kb on - strand, within Pf1N1B4_2197 at 2509.650 kb on + strand, within Pf1N1B4_2197 at 2509.650 kb on + strand at 2509.651 kb on - strand, within Pf1N1B4_2197 at 2509.651 kb on - strand, within Pf1N1B4_2197 at 2509.651 kb on - strand, within Pf1N1B4_2197 at 2509.677 kb on + strand, within Pf1N1B4_2197 at 2509.701 kb on + strand, within Pf1N1B4_2197 at 2509.702 kb on - strand, within Pf1N1B4_2197 at 2509.704 kb on + strand, within Pf1N1B4_2197 at 2509.704 kb on + strand, within Pf1N1B4_2197 at 2509.704 kb on + strand, within Pf1N1B4_2197 at 2509.705 kb on - strand, within Pf1N1B4_2197 at 2509.746 kb on + strand at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.746 kb on + strand, within Pf1N1B4_2197 at 2509.747 kb on - strand, within Pf1N1B4_2197 at 2509.747 kb on - strand, within Pf1N1B4_2197 at 2509.747 kb on - strand, within Pf1N1B4_2197 at 2509.747 kb on - strand, within Pf1N1B4_2197 at 2509.747 kb on - strand, within Pf1N1B4_2197 at 2509.747 kb on - strand, within Pf1N1B4_2197 at 2509.747 kb on - strand, within Pf1N1B4_2197 at 2509.747 kb on - strand, within Pf1N1B4_2197 at 2509.767 kb on + strand at 2509.944 kb on + strand, within Pf1N1B4_2198 at 2509.944 kb on + strand, within Pf1N1B4_2198 at 2509.944 kb on + strand, within Pf1N1B4_2198 at 2509.945 kb on - strand, within Pf1N1B4_2198 at 2509.959 kb on + strand, within Pf1N1B4_2198 at 2509.959 kb on + strand, within Pf1N1B4_2198 at 2509.959 kb on + strand, within Pf1N1B4_2198 at 2509.960 kb on - strand, within Pf1N1B4_2198 at 2510.055 kb on + strand, within Pf1N1B4_2198 at 2510.055 kb on + strand, within Pf1N1B4_2198 at 2510.055 kb on + strand, within Pf1N1B4_2198 at 2510.055 kb on + strand, within Pf1N1B4_2198 at 2510.055 kb on + strand, within Pf1N1B4_2198 at 2510.055 kb on + strand, within Pf1N1B4_2198 at 2510.056 kb on - strand at 2510.056 kb on - strand, within Pf1N1B4_2198 at 2510.056 kb on - strand, within Pf1N1B4_2198 at 2510.190 kb on + strand, within Pf1N1B4_2198 at 2510.190 kb on + strand, within Pf1N1B4_2198 at 2510.191 kb on - strand, within Pf1N1B4_2198 at 2510.191 kb on - strand, within Pf1N1B4_2198 at 2510.191 kb on - strand, within Pf1N1B4_2198 at 2510.191 kb on - strand, within Pf1N1B4_2198 at 2510.218 kb on - strand, within Pf1N1B4_2198 at 2510.349 kb on + strand, within Pf1N1B4_2198 at 2510.349 kb on + strand, within Pf1N1B4_2198 at 2510.350 kb on - strand, within Pf1N1B4_2198 at 2510.350 kb on - strand, within Pf1N1B4_2198 at 2510.350 kb on - strand, within Pf1N1B4_2198 at 2510.470 kb on + strand at 2510.471 kb on - strand at 2510.484 kb on + strand at 2510.565 kb on + strand at 2510.565 kb on + strand, within Pf1N1B4_2199 at 2510.565 kb on + strand, within Pf1N1B4_2199 at 2510.565 kb on + strand, within Pf1N1B4_2199 at 2510.566 kb on - strand at 2510.566 kb on - strand, within Pf1N1B4_2199 at 2510.566 kb on - strand, within Pf1N1B4_2199 at 2510.638 kb on - strand, within Pf1N1B4_2199 at 2510.638 kb on - strand, within Pf1N1B4_2199 at 2510.638 kb on - strand, within Pf1N1B4_2199 at 2510.640 kb on + strand, within Pf1N1B4_2199 at 2510.640 kb on + strand, within Pf1N1B4_2199 at 2510.640 kb on + strand, within Pf1N1B4_2199 at 2510.640 kb on + strand, within Pf1N1B4_2199 at 2510.640 kb on + strand, within Pf1N1B4_2199 at 2510.640 kb on + strand, within Pf1N1B4_2199 at 2510.641 kb on - strand, within Pf1N1B4_2199 at 2510.641 kb on - strand, within Pf1N1B4_2199 at 2510.641 kb on - strand, within Pf1N1B4_2199 at 2510.641 kb on - strand, within Pf1N1B4_2199 at 2510.641 kb on - strand, within Pf1N1B4_2199 at 2510.658 kb on + strand, within Pf1N1B4_2199 at 2510.658 kb on + strand, within Pf1N1B4_2199 at 2510.658 kb on + strand, within Pf1N1B4_2199 at 2510.659 kb on - strand, within Pf1N1B4_2199 at 2510.659 kb on - strand, within Pf1N1B4_2199 at 2510.659 kb on - strand at 2510.871 kb on + strand at 2510.871 kb on + strand at 2510.945 kb on + strand at 2510.945 kb on + strand at 2510.945 kb on + strand at 2510.945 kb on + strand at 2510.946 kb on - strand at 2510.946 kb on - strand at 2511.098 kb on - strand at 2511.106 kb on + strand, within Pf1N1B4_2200 at 2511.106 kb on + strand, within Pf1N1B4_2200 at 2511.106 kb on + strand, within Pf1N1B4_2200 at 2511.106 kb on + strand at 2511.106 kb on + strand, within Pf1N1B4_2200 at 2511.106 kb on + strand, within Pf1N1B4_2200 at 2511.106 kb on + strand, within Pf1N1B4_2200 at 2511.106 kb on + strand, within Pf1N1B4_2200 at 2511.106 kb on + strand, within Pf1N1B4_2200 at 2511.106 kb on + strand, within Pf1N1B4_2200 at 2511.107 kb on - strand, within Pf1N1B4_2200 at 2511.107 kb on - strand, within Pf1N1B4_2200 at 2511.107 kb on - strand, within Pf1N1B4_2200 at 2511.139 kb on + strand, within Pf1N1B4_2200 at 2511.139 kb on + strand, within Pf1N1B4_2200 at 2511.140 kb on - strand, within Pf1N1B4_2200 at 2511.140 kb on - strand, within Pf1N1B4_2200 at 2511.140 kb on - strand, within Pf1N1B4_2200 at 2511.642 kb on + strand, within Pf1N1B4_2200 at 2511.642 kb on + strand, within Pf1N1B4_2200 at 2511.711 kb on + strand, within Pf1N1B4_2200 at 2511.711 kb on + strand, within Pf1N1B4_2200 at 2511.711 kb on + strand, within Pf1N1B4_2200 at 2511.711 kb on + strand, within Pf1N1B4_2200 at 2511.711 kb on + strand, within Pf1N1B4_2200 at 2511.759 kb on + strand, within Pf1N1B4_2200 at 2511.759 kb on + strand, within Pf1N1B4_2200 at 2511.759 kb on + strand, within Pf1N1B4_2200 at 2511.760 kb on - strand, within Pf1N1B4_2200 at 2511.760 kb on - strand, within Pf1N1B4_2200 at 2511.760 kb on - strand, within Pf1N1B4_2200
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 2,509,483 - Pf1N1B4_2197 0.52 +1.3 2,509,483 - Pf1N1B4_2197 0.52 -0.3 2,509,530 + Pf1N1B4_2197 0.58 +0.6 2,509,530 + Pf1N1B4_2197 0.58 -0.8 2,509,530 + Pf1N1B4_2197 0.58 -0.5 2,509,530 + Pf1N1B4_2197 0.58 +0.7 2,509,531 - Pf1N1B4_2197 0.58 +0.3 2,509,531 - Pf1N1B4_2197 0.58 +0.3 2,509,531 - Pf1N1B4_2197 0.58 +1.2 2,509,531 - +0.3 2,509,531 - Pf1N1B4_2197 0.58 -0.5 2,509,531 - Pf1N1B4_2197 0.58 +2.3 2,509,531 - Pf1N1B4_2197 0.58 -1.0 2,509,531 - +0.3 2,509,557 + Pf1N1B4_2197 0.62 +1.3 2,509,558 - Pf1N1B4_2197 0.62 +0.3 2,509,558 - Pf1N1B4_2197 0.62 -0.3 2,509,627 + Pf1N1B4_2197 0.71 -1.4 2,509,627 + Pf1N1B4_2197 0.71 +0.1 2,509,628 - -0.3 2,509,628 - Pf1N1B4_2197 0.71 -0.7 2,509,650 + Pf1N1B4_2197 0.75 +0.1 2,509,650 + -0.3 2,509,651 - Pf1N1B4_2197 0.75 -0.7 2,509,651 - Pf1N1B4_2197 0.75 +0.3 2,509,651 - Pf1N1B4_2197 0.75 -0.1 2,509,677 + Pf1N1B4_2197 0.78 +1.3 2,509,701 + Pf1N1B4_2197 0.82 +0.7 2,509,702 - Pf1N1B4_2197 0.82 -0.3 2,509,704 + Pf1N1B4_2197 0.82 +0.0 2,509,704 + Pf1N1B4_2197 0.82 +0.4 2,509,704 + Pf1N1B4_2197 0.82 +0.7 2,509,705 - Pf1N1B4_2197 0.82 -0.0 2,509,746 + +2.3 2,509,746 + Pf1N1B4_2197 0.88 -0.3 2,509,746 + Pf1N1B4_2197 0.88 -0.5 2,509,746 + Pf1N1B4_2197 0.88 -0.2 2,509,746 + -0.7 2,509,746 + Pf1N1B4_2197 0.88 +1.7 2,509,746 + Pf1N1B4_2197 0.88 -0.8 2,509,746 + Pf1N1B4_2197 0.88 -0.3 2,509,746 + +1.9 2,509,746 + Pf1N1B4_2197 0.88 -0.7 2,509,746 + Pf1N1B4_2197 0.88 -0.5 2,509,746 + Pf1N1B4_2197 0.88 +0.0 2,509,746 + +1.9 2,509,746 + Pf1N1B4_2197 0.88 +1.3 2,509,746 + Pf1N1B4_2197 0.88 +1.3 2,509,746 + Pf1N1B4_2197 0.88 +1.3 2,509,746 + Pf1N1B4_2197 0.88 -2.1 2,509,746 + Pf1N1B4_2197 0.88 +1.5 2,509,746 + Pf1N1B4_2197 0.88 +1.3 2,509,746 + Pf1N1B4_2197 0.88 -0.8 2,509,746 + Pf1N1B4_2197 0.88 -0.3 2,509,747 - Pf1N1B4_2197 0.88 +0.1 2,509,747 - Pf1N1B4_2197 0.88 +1.0 2,509,747 - Pf1N1B4_2197 0.88 -2.0 2,509,747 - Pf1N1B4_2197 0.88 +0.3 2,509,747 - Pf1N1B4_2197 0.88 +1.0 2,509,747 - Pf1N1B4_2197 0.88 +1.1 2,509,747 - Pf1N1B4_2197 0.88 +1.3 2,509,747 - Pf1N1B4_2197 0.88 +0.3 2,509,767 + -1.7 2,509,944 + Pf1N1B4_2198 0.15 +0.7 2,509,944 + Pf1N1B4_2198 0.15 -0.5 2,509,944 + Pf1N1B4_2198 0.15 +0.9 2,509,945 - Pf1N1B4_2198 0.15 -1.6 2,509,959 + Pf1N1B4_2198 0.18 -0.3 2,509,959 + Pf1N1B4_2198 0.18 -1.3 2,509,959 + Pf1N1B4_2198 0.18 -1.8 2,509,960 - Pf1N1B4_2198 0.18 +2.6 2,510,055 + Pf1N1B4_2198 0.34 -0.4 2,510,055 + Pf1N1B4_2198 0.34 -1.0 2,510,055 + Pf1N1B4_2198 0.34 -0.1 2,510,055 + Pf1N1B4_2198 0.34 -1.3 2,510,055 + Pf1N1B4_2198 0.34 +0.6 2,510,055 + Pf1N1B4_2198 0.34 -0.5 2,510,056 - -1.0 2,510,056 - Pf1N1B4_2198 0.34 -0.2 2,510,056 - Pf1N1B4_2198 0.34 -1.3 2,510,190 + Pf1N1B4_2198 0.57 +0.5 2,510,190 + Pf1N1B4_2198 0.57 -0.7 2,510,191 - Pf1N1B4_2198 0.57 +0.0 2,510,191 - Pf1N1B4_2198 0.57 -0.3 2,510,191 - Pf1N1B4_2198 0.57 +2.3 2,510,191 - Pf1N1B4_2198 0.57 -1.5 2,510,218 - Pf1N1B4_2198 0.62 +0.5 2,510,349 + Pf1N1B4_2198 0.84 -1.0 2,510,349 + Pf1N1B4_2198 0.84 -1.0 2,510,350 - Pf1N1B4_2198 0.84 +0.5 2,510,350 - Pf1N1B4_2198 0.84 +0.0 2,510,350 - Pf1N1B4_2198 0.84 +0.8 2,510,470 + +0.6 2,510,471 - +1.0 2,510,484 + -0.6 2,510,565 + +0.7 2,510,565 + Pf1N1B4_2199 0.22 +0.5 2,510,565 + Pf1N1B4_2199 0.22 -1.5 2,510,565 + Pf1N1B4_2199 0.22 -0.7 2,510,566 - -0.3 2,510,566 - Pf1N1B4_2199 0.22 -2.9 2,510,566 - Pf1N1B4_2199 0.22 -1.7 2,510,638 - Pf1N1B4_2199 0.42 +0.6 2,510,638 - Pf1N1B4_2199 0.42 -1.0 2,510,638 - Pf1N1B4_2199 0.42 -2.1 2,510,640 + Pf1N1B4_2199 0.42 -0.3 2,510,640 + Pf1N1B4_2199 0.42 +0.7 2,510,640 + Pf1N1B4_2199 0.42 -2.0 2,510,640 + Pf1N1B4_2199 0.42 -0.3 2,510,640 + Pf1N1B4_2199 0.42 -1.1 2,510,640 + Pf1N1B4_2199 0.42 -0.2 2,510,641 - Pf1N1B4_2199 0.42 -2.0 2,510,641 - Pf1N1B4_2199 0.42 -1.0 2,510,641 - Pf1N1B4_2199 0.42 -2.0 2,510,641 - Pf1N1B4_2199 0.42 +0.1 2,510,641 - Pf1N1B4_2199 0.42 -0.8 2,510,658 + Pf1N1B4_2199 0.47 -0.7 2,510,658 + Pf1N1B4_2199 0.47 -0.5 2,510,658 + Pf1N1B4_2199 0.47 +1.9 2,510,659 - Pf1N1B4_2199 0.47 +0.4 2,510,659 - Pf1N1B4_2199 0.47 -1.4 2,510,659 - +2.9 2,510,871 + +0.1 2,510,871 + -0.3 2,510,945 + +1.3 2,510,945 + +0.6 2,510,945 + +0.2 2,510,945 + -0.7 2,510,946 - +2.3 2,510,946 - +0.9 2,511,098 - +0.7 2,511,106 + Pf1N1B4_2200 0.17 +0.2 2,511,106 + Pf1N1B4_2200 0.17 +1.6 2,511,106 + Pf1N1B4_2200 0.17 -0.7 2,511,106 + +0.7 2,511,106 + Pf1N1B4_2200 0.17 +0.7 2,511,106 + Pf1N1B4_2200 0.17 -1.0 2,511,106 + Pf1N1B4_2200 0.17 -0.7 2,511,106 + Pf1N1B4_2200 0.17 +0.7 2,511,106 + Pf1N1B4_2200 0.17 +0.7 2,511,106 + Pf1N1B4_2200 0.17 -0.7 2,511,107 - Pf1N1B4_2200 0.17 -1.0 2,511,107 - Pf1N1B4_2200 0.17 +1.1 2,511,107 - Pf1N1B4_2200 0.17 +1.3 2,511,139 + Pf1N1B4_2200 0.19 +0.9 2,511,139 + Pf1N1B4_2200 0.19 +1.6 2,511,140 - Pf1N1B4_2200 0.19 +0.5 2,511,140 - Pf1N1B4_2200 0.19 -2.4 2,511,140 - Pf1N1B4_2200 0.19 +0.1 2,511,642 + Pf1N1B4_2200 0.55 +0.3 2,511,642 + Pf1N1B4_2200 0.55 +0.2 2,511,711 + Pf1N1B4_2200 0.60 +0.3 2,511,711 + Pf1N1B4_2200 0.60 -0.4 2,511,711 + Pf1N1B4_2200 0.60 -1.3 2,511,711 + Pf1N1B4_2200 0.60 -0.4 2,511,711 + Pf1N1B4_2200 0.60 -0.8 2,511,759 + Pf1N1B4_2200 0.64 -0.6 2,511,759 + Pf1N1B4_2200 0.64 +0.3 2,511,759 + Pf1N1B4_2200 0.64 -1.0 2,511,760 - Pf1N1B4_2200 0.64 -0.6 2,511,760 - Pf1N1B4_2200 0.64 +0.3 2,511,760 - Pf1N1B4_2200 0.64 -0.3
Or see this region's nucleotide sequence