Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2058 and Pf1N1B4_2059 overlap by 4 nucleotides Pf1N1B4_2059 and Pf1N1B4_2060 overlap by 7 nucleotides Pf1N1B4_2060 and Pf1N1B4_2061 are separated by 577 nucleotides
Pf1N1B4_2058 - Protein ImpG/VasA, at 2,350,276 to 2,352,135
_2058
Pf1N1B4_2059 - Uncharacterized protein ImpF, at 2,352,132 to 2,352,644
_2059
Pf1N1B4_2060 - hypothetical protein, at 2,352,638 to 2,353,063
_2060
Pf1N1B4_2061 - hypothetical protein, at 2,353,641 to 2,354,078
_2061
Position (kb)
2352
2353
2354 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2351.694 kb on - strand, within Pf1N1B4_2058 at 2351.694 kb on - strand, within Pf1N1B4_2058 at 2351.888 kb on + strand, within Pf1N1B4_2058 at 2351.889 kb on - strand, within Pf1N1B4_2058 at 2351.956 kb on + strand at 2351.957 kb on - strand at 2352.002 kb on + strand at 2352.002 kb on + strand at 2352.110 kb on + strand at 2352.110 kb on + strand at 2352.110 kb on + strand at 2352.110 kb on + strand at 2352.110 kb on + strand at 2352.111 kb on - strand at 2352.111 kb on - strand at 2352.111 kb on - strand at 2352.111 kb on - strand at 2352.111 kb on - strand at 2352.111 kb on - strand at 2352.646 kb on + strand at 2352.646 kb on + strand at 2352.647 kb on - strand at 2352.647 kb on - strand at 2352.660 kb on + strand at 2352.660 kb on + strand at 2352.660 kb on + strand at 2352.660 kb on + strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.661 kb on - strand at 2352.703 kb on + strand, within Pf1N1B4_2060 at 2352.743 kb on - strand, within Pf1N1B4_2060 at 2352.854 kb on - strand, within Pf1N1B4_2060 at 2353.210 kb on + strand at 2353.211 kb on - strand at 2353.218 kb on + strand at 2353.320 kb on + strand at 2353.396 kb on + strand at 2353.430 kb on + strand at 2353.431 kb on - strand at 2353.431 kb on - strand at 2353.507 kb on + strand at 2353.508 kb on - strand at 2353.512 kb on + strand at 2353.512 kb on + strand at 2353.512 kb on + strand at 2353.512 kb on + strand at 2353.513 kb on - strand at 2353.513 kb on - strand at 2353.640 kb on - strand at 2353.719 kb on + strand, within Pf1N1B4_2061 at 2353.719 kb on + strand, within Pf1N1B4_2061 at 2353.770 kb on - strand, within Pf1N1B4_2061 at 2353.770 kb on - strand, within Pf1N1B4_2061 at 2353.933 kb on + strand, within Pf1N1B4_2061 at 2353.933 kb on + strand, within Pf1N1B4_2061 at 2353.934 kb on - strand, within Pf1N1B4_2061 at 2353.934 kb on - strand, within Pf1N1B4_2061 at 2353.985 kb on + strand, within Pf1N1B4_2061 at 2353.986 kb on - strand, within Pf1N1B4_2061 at 2354.057 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 2,351,694 - Pf1N1B4_2058 0.76 -0.7 2,351,694 - Pf1N1B4_2058 0.76 +0.3 2,351,888 + Pf1N1B4_2058 0.87 -1.1 2,351,889 - Pf1N1B4_2058 0.87 +0.8 2,351,956 + +1.3 2,351,957 - -0.2 2,352,002 + +0.3 2,352,002 + +0.7 2,352,110 + -0.1 2,352,110 + -1.0 2,352,110 + -0.6 2,352,110 + -2.1 2,352,110 + +0.0 2,352,111 - -1.2 2,352,111 - -1.7 2,352,111 - +1.0 2,352,111 - +0.6 2,352,111 - -0.3 2,352,111 - +0.6 2,352,646 + +0.9 2,352,646 + +0.8 2,352,647 - -0.7 2,352,647 - -0.7 2,352,660 + -1.0 2,352,660 + +0.9 2,352,660 + +0.9 2,352,660 + -0.1 2,352,661 - +0.9 2,352,661 - -0.2 2,352,661 - +1.3 2,352,661 - +0.3 2,352,661 - +1.1 2,352,661 - -0.2 2,352,661 - -0.7 2,352,661 - +0.6 2,352,703 + Pf1N1B4_2060 0.15 -0.4 2,352,743 - Pf1N1B4_2060 0.25 -2.0 2,352,854 - Pf1N1B4_2060 0.51 -1.5 2,353,210 + +0.7 2,353,211 - +1.1 2,353,218 + -0.2 2,353,320 + +1.9 2,353,396 + +0.3 2,353,430 + -1.0 2,353,431 - +0.7 2,353,431 - -0.2 2,353,507 + +0.7 2,353,508 - -1.7 2,353,512 + -1.7 2,353,512 + +1.0 2,353,512 + +0.3 2,353,512 + +1.6 2,353,513 - +1.8 2,353,513 - -1.3 2,353,640 - -1.0 2,353,719 + Pf1N1B4_2061 0.18 -1.0 2,353,719 + Pf1N1B4_2061 0.18 -2.0 2,353,770 - Pf1N1B4_2061 0.29 +0.6 2,353,770 - Pf1N1B4_2061 0.29 -0.1 2,353,933 + Pf1N1B4_2061 0.67 +1.3 2,353,933 + Pf1N1B4_2061 0.67 +1.3 2,353,934 - Pf1N1B4_2061 0.67 +0.8 2,353,934 - Pf1N1B4_2061 0.67 +0.3 2,353,985 + Pf1N1B4_2061 0.79 +2.2 2,353,986 - Pf1N1B4_2061 0.79 +0.2 2,354,057 + -0.1
Or see this region's nucleotide sequence