Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1674 and Pf1N1B4_1675 are separated by 124 nucleotides Pf1N1B4_1675 and Pf1N1B4_1676 are separated by 250 nucleotides
Pf1N1B4_1674 - Putative formate dehydrogenase oxidoreductase protein, at 1,917,101 to 1,919,449
_1674
Pf1N1B4_1675 - Transcriptional regulator, at 1,919,574 to 1,920,494
_1675
Pf1N1B4_1676 - Inner membrane component of tripartite multidrug resistance system, at 1,920,745 to 1,922,283
_1676
Position (kb)
1919
1920
1921 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1918.634 kb on - strand, within Pf1N1B4_1674 at 1918.674 kb on + strand, within Pf1N1B4_1674 at 1918.859 kb on + strand, within Pf1N1B4_1674 at 1918.860 kb on - strand, within Pf1N1B4_1674 at 1918.943 kb on + strand, within Pf1N1B4_1674 at 1918.944 kb on - strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.028 kb on + strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.029 kb on - strand, within Pf1N1B4_1674 at 1919.102 kb on + strand at 1919.102 kb on + strand, within Pf1N1B4_1674 at 1919.102 kb on + strand, within Pf1N1B4_1674 at 1919.103 kb on - strand, within Pf1N1B4_1674 at 1919.103 kb on - strand, within Pf1N1B4_1674 at 1919.103 kb on - strand, within Pf1N1B4_1674 at 1919.117 kb on + strand, within Pf1N1B4_1674 at 1919.117 kb on + strand at 1919.118 kb on - strand, within Pf1N1B4_1674 at 1919.118 kb on - strand, within Pf1N1B4_1674 at 1919.118 kb on - strand, within Pf1N1B4_1674 at 1919.315 kb on + strand at 1919.315 kb on + strand at 1919.315 kb on + strand at 1919.316 kb on - strand at 1919.316 kb on - strand at 1919.316 kb on - strand at 1919.318 kb on + strand at 1919.371 kb on + strand at 1919.371 kb on + strand at 1919.372 kb on - strand at 1919.505 kb on + strand at 1919.505 kb on + strand at 1919.505 kb on + strand at 1919.911 kb on + strand, within Pf1N1B4_1675 at 1919.911 kb on + strand, within Pf1N1B4_1675 at 1919.911 kb on + strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1919.912 kb on - strand, within Pf1N1B4_1675 at 1920.539 kb on + strand at 1920.539 kb on + strand at 1920.539 kb on + strand at 1920.540 kb on - strand at 1920.551 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.663 kb on + strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.664 kb on - strand at 1920.665 kb on + strand at 1920.665 kb on + strand at 1920.666 kb on - strand at 1920.666 kb on - strand at 1920.666 kb on - strand at 1920.666 kb on - strand at 1920.676 kb on + strand at 1920.708 kb on - strand at 1920.743 kb on + strand at 1920.852 kb on - strand at 1920.964 kb on + strand, within Pf1N1B4_1676 at 1920.964 kb on + strand, within Pf1N1B4_1676 at 1920.965 kb on - strand, within Pf1N1B4_1676 at 1920.965 kb on - strand at 1920.965 kb on - strand, within Pf1N1B4_1676 at 1921.042 kb on + strand, within Pf1N1B4_1676 at 1921.096 kb on + strand, within Pf1N1B4_1676 at 1921.097 kb on - strand, within Pf1N1B4_1676 at 1921.204 kb on + strand, within Pf1N1B4_1676 at 1921.204 kb on + strand, within Pf1N1B4_1676 at 1921.205 kb on - strand, within Pf1N1B4_1676 at 1921.205 kb on - strand, within Pf1N1B4_1676 at 1921.282 kb on + strand, within Pf1N1B4_1676 at 1921.282 kb on + strand, within Pf1N1B4_1676 at 1921.282 kb on + strand, within Pf1N1B4_1676 at 1921.283 kb on - strand, within Pf1N1B4_1676
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 1,918,634 - Pf1N1B4_1674 0.65 -0.1 1,918,674 + Pf1N1B4_1674 0.67 +1.1 1,918,859 + Pf1N1B4_1674 0.75 -0.5 1,918,860 - Pf1N1B4_1674 0.75 -0.1 1,918,943 + Pf1N1B4_1674 0.78 +0.5 1,918,944 - Pf1N1B4_1674 0.78 +1.3 1,919,028 + Pf1N1B4_1674 0.82 +0.3 1,919,028 + Pf1N1B4_1674 0.82 -0.4 1,919,028 + Pf1N1B4_1674 0.82 +0.2 1,919,028 + Pf1N1B4_1674 0.82 -1.0 1,919,028 + Pf1N1B4_1674 0.82 +1.3 1,919,028 + Pf1N1B4_1674 0.82 -0.7 1,919,028 + Pf1N1B4_1674 0.82 +0.7 1,919,028 + Pf1N1B4_1674 0.82 +0.2 1,919,028 + Pf1N1B4_1674 0.82 -0.5 1,919,028 + Pf1N1B4_1674 0.82 +0.9 1,919,028 + Pf1N1B4_1674 0.82 -0.7 1,919,029 - Pf1N1B4_1674 0.82 +1.0 1,919,029 - Pf1N1B4_1674 0.82 +0.3 1,919,029 - Pf1N1B4_1674 0.82 -0.1 1,919,029 - Pf1N1B4_1674 0.82 +1.2 1,919,029 - Pf1N1B4_1674 0.82 -0.5 1,919,029 - Pf1N1B4_1674 0.82 +1.6 1,919,029 - Pf1N1B4_1674 0.82 -0.3 1,919,029 - Pf1N1B4_1674 0.82 +0.0 1,919,029 - Pf1N1B4_1674 0.82 -1.0 1,919,029 - Pf1N1B4_1674 0.82 -0.3 1,919,029 - Pf1N1B4_1674 0.82 +0.5 1,919,029 - Pf1N1B4_1674 0.82 +1.9 1,919,102 + +2.3 1,919,102 + Pf1N1B4_1674 0.85 +0.6 1,919,102 + Pf1N1B4_1674 0.85 -0.3 1,919,103 - Pf1N1B4_1674 0.85 -2.5 1,919,103 - Pf1N1B4_1674 0.85 -0.3 1,919,103 - Pf1N1B4_1674 0.85 +0.9 1,919,117 + Pf1N1B4_1674 0.86 -0.4 1,919,117 + +0.3 1,919,118 - Pf1N1B4_1674 0.86 +0.6 1,919,118 - Pf1N1B4_1674 0.86 -0.4 1,919,118 - Pf1N1B4_1674 0.86 -0.6 1,919,315 + +0.2 1,919,315 + -0.1 1,919,315 + -0.3 1,919,316 - -0.7 1,919,316 - -0.4 1,919,316 - +0.6 1,919,318 + +0.6 1,919,371 + -1.3 1,919,371 + +1.3 1,919,372 - +0.3 1,919,505 + -1.0 1,919,505 + -0.3 1,919,505 + -0.7 1,919,911 + Pf1N1B4_1675 0.37 -0.7 1,919,911 + Pf1N1B4_1675 0.37 -1.0 1,919,911 + Pf1N1B4_1675 0.37 -1.3 1,919,912 - Pf1N1B4_1675 0.37 +0.3 1,919,912 - Pf1N1B4_1675 0.37 -1.5 1,919,912 - Pf1N1B4_1675 0.37 -0.1 1,919,912 - Pf1N1B4_1675 0.37 -2.3 1,920,539 + -0.3 1,920,539 + +0.8 1,920,539 + +0.2 1,920,540 - -0.3 1,920,551 + -0.5 1,920,663 + +1.2 1,920,663 + +0.0 1,920,663 + +1.1 1,920,663 + -1.9 1,920,663 + -1.8 1,920,663 + +0.4 1,920,663 + +1.1 1,920,663 + +0.0 1,920,663 + -1.1 1,920,663 + -0.1 1,920,663 + -0.8 1,920,664 - +1.7 1,920,664 - +0.7 1,920,664 - +1.3 1,920,664 - -0.8 1,920,664 - +1.3 1,920,664 - +1.6 1,920,665 + +0.5 1,920,665 + +0.3 1,920,666 - +0.9 1,920,666 - +0.1 1,920,666 - +0.7 1,920,666 - -0.1 1,920,676 + +0.7 1,920,708 - -0.8 1,920,743 + +0.6 1,920,852 - -1.5 1,920,964 + Pf1N1B4_1676 0.14 -0.5 1,920,964 + Pf1N1B4_1676 0.14 +0.1 1,920,965 - Pf1N1B4_1676 0.14 -0.3 1,920,965 - +0.0 1,920,965 - Pf1N1B4_1676 0.14 -1.7 1,921,042 + Pf1N1B4_1676 0.19 -0.5 1,921,096 + Pf1N1B4_1676 0.23 -0.7 1,921,097 - Pf1N1B4_1676 0.23 -1.0 1,921,204 + Pf1N1B4_1676 0.30 -0.3 1,921,204 + Pf1N1B4_1676 0.30 +2.3 1,921,205 - Pf1N1B4_1676 0.30 -1.7 1,921,205 - Pf1N1B4_1676 0.30 +2.6 1,921,282 + Pf1N1B4_1676 0.35 +0.7 1,921,282 + Pf1N1B4_1676 0.35 -0.3 1,921,282 + Pf1N1B4_1676 0.35 +0.3 1,921,283 - Pf1N1B4_1676 0.35 +0.7
Or see this region's nucleotide sequence