Experiment: Cholin acetate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_122 and Pf1N1B4_123 are separated by 9 nucleotides Pf1N1B4_123 and Pf1N1B4_124 are separated by 245 nucleotides Pf1N1B4_124 and Pf1N1B4_125 are separated by 189 nucleotides Pf1N1B4_125 and Pf1N1B4_126 are separated by 127 nucleotides
Pf1N1B4_122 - Inner membrane component of tripartite multidrug resistance system, at 133,290 to 134,882
_122
Pf1N1B4_123 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 134,892 to 135,332
_123
Pf1N1B4_124 - Mechanosensitive ion channel, at 135,578 to 137,023
_124
Pf1N1B4_125 - Nicotinamidase family protein YcaC, at 137,213 to 137,839
_125
Pf1N1B4_126 - Pirin, at 137,967 to 138,833
_126
Position (kb)
135
136
137
138 Strain fitness (log2 ratio)
-1
0
1
2 at 134.675 kb on + strand, within Pf1N1B4_122 at 134.676 kb on - strand at 134.772 kb on + strand at 134.772 kb on + strand at 134.772 kb on + strand at 134.773 kb on - strand at 134.894 kb on - strand at 135.011 kb on - strand, within Pf1N1B4_123 at 135.011 kb on - strand, within Pf1N1B4_123 at 135.311 kb on - strand at 135.389 kb on - strand at 135.389 kb on - strand at 135.394 kb on + strand at 135.403 kb on - strand at 135.403 kb on - strand at 135.425 kb on + strand at 135.526 kb on + strand at 135.735 kb on + strand, within Pf1N1B4_124 at 136.647 kb on + strand, within Pf1N1B4_124 at 136.647 kb on + strand, within Pf1N1B4_124 at 136.648 kb on - strand, within Pf1N1B4_124 at 136.648 kb on - strand, within Pf1N1B4_124 at 137.040 kb on + strand at 137.041 kb on - strand at 137.043 kb on + strand at 137.044 kb on - strand at 137.044 kb on - strand at 137.060 kb on + strand at 137.061 kb on - strand at 137.061 kb on - strand at 137.061 kb on - strand at 137.128 kb on - strand at 137.189 kb on + strand at 137.189 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.197 kb on + strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.198 kb on - strand at 137.214 kb on + strand at 137.215 kb on - strand at 137.238 kb on + strand at 137.239 kb on - strand at 137.239 kb on - strand at 137.695 kb on - strand, within Pf1N1B4_125 at 137.834 kb on - strand at 137.834 kb on - strand at 137.924 kb on + strand at 137.955 kb on - strand at 137.955 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cholin acetate 10 mM remove 134,675 + Pf1N1B4_122 0.87 -0.8 134,676 - +1.7 134,772 + +0.7 134,772 + +0.7 134,772 + -0.3 134,773 - +1.7 134,894 - +0.2 135,011 - Pf1N1B4_123 0.27 +0.2 135,011 - Pf1N1B4_123 0.27 +0.6 135,311 - -0.3 135,389 - +0.3 135,389 - -0.6 135,394 + +1.9 135,403 - +0.2 135,403 - +0.0 135,425 + -1.3 135,526 + +0.6 135,735 + Pf1N1B4_124 0.11 -0.5 136,647 + Pf1N1B4_124 0.74 -0.4 136,647 + Pf1N1B4_124 0.74 -0.8 136,648 - Pf1N1B4_124 0.74 +0.7 136,648 - Pf1N1B4_124 0.74 -1.6 137,040 + -0.3 137,041 - +0.4 137,043 + +0.9 137,044 - -1.1 137,044 - +0.3 137,060 + +0.3 137,061 - +1.7 137,061 - +2.3 137,061 - +1.2 137,128 - +0.3 137,189 + +1.2 137,189 + -0.1 137,197 + +0.7 137,197 + +1.6 137,197 + +0.7 137,197 + +0.7 137,197 + -0.3 137,198 - -0.3 137,198 - -0.3 137,198 - +2.7 137,198 - -0.3 137,198 - -0.3 137,214 + +0.0 137,215 - -0.3 137,238 + -0.7 137,239 - +1.3 137,239 - +0.0 137,695 - Pf1N1B4_125 0.77 -0.3 137,834 - +0.2 137,834 - +2.3 137,924 + -0.6 137,955 - -0.6 137,955 - +0.0
Or see this region's nucleotide sequence