Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_124

Experiment: Cholin acetate 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_122 and Pf1N1B4_123 are separated by 9 nucleotidesPf1N1B4_123 and Pf1N1B4_124 are separated by 245 nucleotidesPf1N1B4_124 and Pf1N1B4_125 are separated by 189 nucleotidesPf1N1B4_125 and Pf1N1B4_126 are separated by 127 nucleotides Pf1N1B4_122 - Inner membrane component of tripartite multidrug resistance system, at 133,290 to 134,882 _122 Pf1N1B4_123 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 134,892 to 135,332 _123 Pf1N1B4_124 - Mechanosensitive ion channel, at 135,578 to 137,023 _124 Pf1N1B4_125 - Nicotinamidase family protein YcaC, at 137,213 to 137,839 _125 Pf1N1B4_126 - Pirin, at 137,967 to 138,833 _126 Position (kb) 135 136 137 138Strain fitness (log2 ratio) -1 0 1 2at 134.675 kb on + strand, within Pf1N1B4_122at 134.676 kb on - strandat 134.772 kb on + strandat 134.772 kb on + strandat 134.772 kb on + strandat 134.773 kb on - strandat 134.894 kb on - strandat 135.011 kb on - strand, within Pf1N1B4_123at 135.011 kb on - strand, within Pf1N1B4_123at 135.311 kb on - strandat 135.389 kb on - strandat 135.389 kb on - strandat 135.394 kb on + strandat 135.403 kb on - strandat 135.403 kb on - strandat 135.425 kb on + strandat 135.526 kb on + strandat 135.735 kb on + strand, within Pf1N1B4_124at 136.647 kb on + strand, within Pf1N1B4_124at 136.647 kb on + strand, within Pf1N1B4_124at 136.648 kb on - strand, within Pf1N1B4_124at 136.648 kb on - strand, within Pf1N1B4_124at 137.040 kb on + strandat 137.041 kb on - strandat 137.043 kb on + strandat 137.044 kb on - strandat 137.044 kb on - strandat 137.060 kb on + strandat 137.061 kb on - strandat 137.061 kb on - strandat 137.061 kb on - strandat 137.128 kb on - strandat 137.189 kb on + strandat 137.189 kb on + strandat 137.197 kb on + strandat 137.197 kb on + strandat 137.197 kb on + strandat 137.197 kb on + strandat 137.197 kb on + strandat 137.198 kb on - strandat 137.198 kb on - strandat 137.198 kb on - strandat 137.198 kb on - strandat 137.198 kb on - strandat 137.214 kb on + strandat 137.215 kb on - strandat 137.238 kb on + strandat 137.239 kb on - strandat 137.239 kb on - strandat 137.695 kb on - strand, within Pf1N1B4_125at 137.834 kb on - strandat 137.834 kb on - strandat 137.924 kb on + strandat 137.955 kb on - strandat 137.955 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cholin acetate 10 mM
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134,675 + Pf1N1B4_122 0.87 -0.8
134,676 - +1.7
134,772 + +0.7
134,772 + +0.7
134,772 + -0.3
134,773 - +1.7
134,894 - +0.2
135,011 - Pf1N1B4_123 0.27 +0.2
135,011 - Pf1N1B4_123 0.27 +0.6
135,311 - -0.3
135,389 - +0.3
135,389 - -0.6
135,394 + +1.9
135,403 - +0.2
135,403 - +0.0
135,425 + -1.3
135,526 + +0.6
135,735 + Pf1N1B4_124 0.11 -0.5
136,647 + Pf1N1B4_124 0.74 -0.4
136,647 + Pf1N1B4_124 0.74 -0.8
136,648 - Pf1N1B4_124 0.74 +0.7
136,648 - Pf1N1B4_124 0.74 -1.6
137,040 + -0.3
137,041 - +0.4
137,043 + +0.9
137,044 - -1.1
137,044 - +0.3
137,060 + +0.3
137,061 - +1.7
137,061 - +2.3
137,061 - +1.2
137,128 - +0.3
137,189 + +1.2
137,189 + -0.1
137,197 + +0.7
137,197 + +1.6
137,197 + +0.7
137,197 + +0.7
137,197 + -0.3
137,198 - -0.3
137,198 - -0.3
137,198 - +2.7
137,198 - -0.3
137,198 - -0.3
137,214 + +0.0
137,215 - -0.3
137,238 + -0.7
137,239 - +1.3
137,239 - +0.0
137,695 - Pf1N1B4_125 0.77 -0.3
137,834 - +0.2
137,834 - +2.3
137,924 + -0.6
137,955 - -0.6
137,955 - +0.0

Or see this region's nucleotide sequence