Experiment: Chloramphenicol 0.012 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR86_RS01075 and ABZR86_RS01080 are separated by 70 nucleotides ABZR86_RS01080 and ABZR86_RS01085 are separated by 77 nucleotides ABZR86_RS01085 and ABZR86_RS01090 are separated by 198 nucleotides
ABZR86_RS01075: ABZR86_RS01075 - hypothetical protein, at 214,612 to 214,956
_RS01075
ABZR86_RS01080: ABZR86_RS01080 - hypothetical protein, at 215,027 to 215,227
_RS01080
ABZR86_RS01085: ABZR86_RS01085 - GlsB/YeaQ/YmgE family stress response membrane protein, at 215,305 to 215,556
_RS01085
ABZR86_RS01090: ABZR86_RS01090 - enoyl-ACP reductase FabV, at 215,755 to 216,954
_RS01090
Position (kb)
215
216 Strain fitness (log2 ratio)
-2
-1
0
1 at 214.584 kb on + strand at 214.584 kb on + strand at 214.584 kb on + strand at 214.584 kb on + strand at 214.584 kb on + strand at 214.584 kb on + strand at 214.585 kb on - strand at 214.585 kb on - strand at 214.585 kb on - strand at 214.585 kb on - strand at 214.585 kb on - strand at 214.952 kb on - strand at 215.028 kb on + strand at 215.028 kb on + strand at 215.029 kb on - strand at 215.050 kb on + strand, within ABZR86_RS01080 at 215.051 kb on - strand, within ABZR86_RS01080 at 215.051 kb on - strand, within ABZR86_RS01080 at 215.591 kb on + strand at 215.591 kb on + strand at 215.592 kb on - strand at 215.621 kb on + strand at 215.621 kb on + strand at 215.621 kb on + strand at 215.621 kb on + strand at 215.621 kb on + strand at 215.622 kb on - strand at 215.622 kb on - strand at 215.622 kb on - strand at 215.622 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.012 mg/ml remove 214,584 + +0.1 214,584 + -0.8 214,584 + +0.7 214,584 + -0.4 214,584 + +0.2 214,584 + -2.1 214,585 - +0.8 214,585 - -2.1 214,585 - -0.5 214,585 - -1.2 214,585 - +1.0 214,952 - -0.7 215,028 + -1.0 215,028 + -1.4 215,029 - +0.9 215,050 + ABZR86_RS01080 0.11 -0.1 215,051 - ABZR86_RS01080 0.12 -0.4 215,051 - ABZR86_RS01080 0.12 -0.7 215,591 + +0.3 215,591 + -0.1 215,592 - +0.5 215,621 + +0.8 215,621 + -0.8 215,621 + +0.9 215,621 + +0.4 215,621 + +0.5 215,622 - -0.7 215,622 - +0.1 215,622 - +0.2 215,622 - -0.3
Or see this region's nucleotide sequence