Experiment: nitrate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purU and Synpcc7942_1009 are separated by 164 nucleotides Synpcc7942_1009 and Synpcc7942_1010 are separated by 18 nucleotides Synpcc7942_1010 and sphS are separated by 14 nucleotides
Synpcc7942_1008: purU - formyltetrahydrofolate deformylase, at 1,020,332 to 1,021,186
purU
Synpcc7942_1009: Synpcc7942_1009 - transcriptional regulator, XRE family, at 1,021,351 to 1,021,950
_1009
Synpcc7942_1010: Synpcc7942_1010 - ribosomal large subunit pseudouridine synthase B, at 1,021,969 to 1,022,700
_1010
Synpcc7942_1011: sphS - PAS/PAC sensor signal transduction histidine kinase, at 1,022,715 to 1,023,956
sphS
Position (kb)
1021
1022
1023 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1021.033 kb on - strand, within purU at 1021.033 kb on - strand, within purU at 1021.054 kb on - strand, within purU at 1021.083 kb on + strand, within purU at 1021.152 kb on - strand at 1021.157 kb on + strand at 1021.227 kb on + strand at 1021.268 kb on - strand at 1021.313 kb on + strand at 1021.314 kb on - strand at 1021.350 kb on - strand at 1021.351 kb on + strand at 1021.353 kb on + strand at 1021.361 kb on + strand at 1021.362 kb on + strand at 1021.369 kb on - strand at 1021.369 kb on - strand at 1021.423 kb on - strand, within Synpcc7942_1009 at 1021.973 kb on - strand at 1022.070 kb on + strand, within Synpcc7942_1010 at 1022.083 kb on + strand, within Synpcc7942_1010 at 1022.171 kb on - strand, within Synpcc7942_1010 at 1022.172 kb on - strand, within Synpcc7942_1010 at 1022.196 kb on - strand, within Synpcc7942_1010 at 1022.206 kb on - strand, within Synpcc7942_1010 at 1022.208 kb on + strand, within Synpcc7942_1010 at 1022.219 kb on - strand, within Synpcc7942_1010 at 1022.220 kb on + strand, within Synpcc7942_1010 at 1022.340 kb on - strand, within Synpcc7942_1010 at 1022.353 kb on + strand, within Synpcc7942_1010 at 1022.353 kb on + strand, within Synpcc7942_1010 at 1022.359 kb on + strand, within Synpcc7942_1010 at 1022.359 kb on + strand, within Synpcc7942_1010 at 1022.361 kb on - strand, within Synpcc7942_1010 at 1022.361 kb on - strand, within Synpcc7942_1010 at 1022.363 kb on - strand, within Synpcc7942_1010 at 1022.376 kb on - strand, within Synpcc7942_1010 at 1022.378 kb on + strand, within Synpcc7942_1010 at 1022.378 kb on + strand, within Synpcc7942_1010 at 1022.386 kb on - strand, within Synpcc7942_1010 at 1022.386 kb on - strand, within Synpcc7942_1010 at 1022.401 kb on - strand, within Synpcc7942_1010 at 1022.467 kb on + strand, within Synpcc7942_1010 at 1022.475 kb on - strand, within Synpcc7942_1010 at 1022.480 kb on - strand, within Synpcc7942_1010 at 1022.486 kb on + strand, within Synpcc7942_1010 at 1022.486 kb on + strand, within Synpcc7942_1010 at 1022.486 kb on + strand, within Synpcc7942_1010 at 1022.494 kb on - strand, within Synpcc7942_1010 at 1022.663 kb on + strand at 1022.685 kb on + strand at 1022.704 kb on - strand at 1022.706 kb on + strand at 1022.706 kb on + strand at 1022.714 kb on - strand at 1022.714 kb on - strand at 1022.716 kb on - strand at 1022.729 kb on + strand at 1022.729 kb on + strand at 1022.729 kb on + strand at 1022.737 kb on - strand at 1022.754 kb on + strand at 1022.754 kb on + strand at 1022.755 kb on - strand at 1022.755 kb on - strand at 1022.760 kb on - strand at 1022.777 kb on + strand at 1022.777 kb on + strand at 1022.785 kb on - strand at 1022.873 kb on - strand at 1022.892 kb on + strand, within sphS at 1022.892 kb on + strand, within sphS at 1022.919 kb on - strand, within sphS at 1022.920 kb on + strand, within sphS at 1022.943 kb on - strand, within sphS at 1022.943 kb on - strand, within sphS at 1022.978 kb on + strand, within sphS at 1022.978 kb on + strand, within sphS at 1022.981 kb on - strand, within sphS at 1022.981 kb on - strand, within sphS at 1022.986 kb on - strand, within sphS at 1022.991 kb on + strand, within sphS at 1023.006 kb on + strand, within sphS at 1023.014 kb on - strand, within sphS at 1023.014 kb on - strand, within sphS at 1023.026 kb on + strand at 1023.026 kb on + strand, within sphS at 1023.032 kb on + strand, within sphS at 1023.032 kb on + strand, within sphS at 1023.040 kb on - strand, within sphS at 1023.040 kb on - strand, within sphS at 1023.045 kb on - strand, within sphS at 1023.133 kb on - strand, within sphS at 1023.138 kb on - strand at 1023.140 kb on - strand, within sphS at 1023.145 kb on - strand, within sphS at 1023.154 kb on + strand, within sphS at 1023.180 kb on - strand, within sphS at 1023.185 kb on + strand, within sphS at 1023.185 kb on + strand, within sphS at 1023.185 kb on + strand at 1023.185 kb on + strand, within sphS at 1023.193 kb on - strand, within sphS at 1023.193 kb on - strand, within sphS at 1023.193 kb on - strand, within sphS at 1023.201 kb on - strand, within sphS at 1023.223 kb on - strand, within sphS at 1023.225 kb on + strand, within sphS at 1023.261 kb on - strand, within sphS at 1023.272 kb on + strand, within sphS at 1023.273 kb on + strand, within sphS at 1023.281 kb on - strand, within sphS at 1023.337 kb on + strand, within sphS at 1023.374 kb on + strand, within sphS at 1023.384 kb on - strand, within sphS at 1023.460 kb on + strand, within sphS at 1023.462 kb on + strand, within sphS at 1023.462 kb on + strand, within sphS at 1023.465 kb on - strand, within sphS at 1023.494 kb on - strand, within sphS at 1023.506 kb on - strand, within sphS at 1023.528 kb on + strand, within sphS at 1023.528 kb on + strand, within sphS at 1023.528 kb on + strand, within sphS at 1023.528 kb on + strand, within sphS at 1023.528 kb on + strand, within sphS at 1023.536 kb on - strand, within sphS at 1023.536 kb on - strand, within sphS at 1023.536 kb on - strand, within sphS at 1023.541 kb on - strand, within sphS at 1023.547 kb on - strand, within sphS at 1023.554 kb on + strand, within sphS at 1023.554 kb on + strand, within sphS at 1023.567 kb on - strand, within sphS at 1023.574 kb on + strand, within sphS at 1023.593 kb on - strand, within sphS at 1023.619 kb on - strand, within sphS at 1023.621 kb on - strand, within sphS at 1023.650 kb on + strand, within sphS at 1023.668 kb on + strand, within sphS at 1023.668 kb on + strand, within sphS at 1023.668 kb on + strand, within sphS at 1023.675 kb on - strand, within sphS at 1023.675 kb on - strand, within sphS at 1023.684 kb on + strand, within sphS at 1023.685 kb on + strand, within sphS
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 50 mM remove 1,021,033 - purU Synpcc7942_1008 0.82 -0.1 1,021,033 - purU Synpcc7942_1008 0.82 -1.7 1,021,054 - purU Synpcc7942_1008 0.84 -1.1 1,021,083 + purU Synpcc7942_1008 0.88 +1.1 1,021,152 - -2.7 1,021,157 + -0.1 1,021,227 + -1.1 1,021,268 - -0.1 1,021,313 + +0.5 1,021,314 - +4.0 1,021,350 - +3.6 1,021,351 + +1.9 1,021,353 + -1.1 1,021,361 + +0.5 1,021,362 + +3.6 1,021,369 - +0.5 1,021,369 - -1.7 1,021,423 - Synpcc7942_1009 0.12 -0.3 1,021,973 - +1.7 1,022,070 + Synpcc7942_1010 0.14 -0.1 1,022,083 + Synpcc7942_1010 0.16 +4.3 1,022,171 - Synpcc7942_1010 0.28 +2.6 1,022,172 - Synpcc7942_1010 0.28 -1.1 1,022,196 - Synpcc7942_1010 0.31 +4.1 1,022,206 - Synpcc7942_1010 0.32 -0.5 1,022,208 + Synpcc7942_1010 0.33 -0.4 1,022,219 - Synpcc7942_1010 0.34 -0.1 1,022,220 + Synpcc7942_1010 0.34 +4.5 1,022,340 - Synpcc7942_1010 0.51 +4.1 1,022,353 + Synpcc7942_1010 0.52 -0.9 1,022,353 + Synpcc7942_1010 0.52 +3.0 1,022,359 + Synpcc7942_1010 0.53 +1.1 1,022,359 + Synpcc7942_1010 0.53 +4.2 1,022,361 - Synpcc7942_1010 0.54 -1.1 1,022,361 - Synpcc7942_1010 0.54 +0.3 1,022,363 - Synpcc7942_1010 0.54 -1.1 1,022,376 - Synpcc7942_1010 0.56 -0.1 1,022,378 + Synpcc7942_1010 0.56 -2.5 1,022,378 + Synpcc7942_1010 0.56 +4.7 1,022,386 - Synpcc7942_1010 0.57 +3.3 1,022,386 - Synpcc7942_1010 0.57 -1.1 1,022,401 - Synpcc7942_1010 0.59 +0.3 1,022,467 + Synpcc7942_1010 0.68 +0.9 1,022,475 - Synpcc7942_1010 0.69 -2.1 1,022,480 - Synpcc7942_1010 0.70 +0.3 1,022,486 + Synpcc7942_1010 0.71 +1.5 1,022,486 + Synpcc7942_1010 0.71 -0.1 1,022,486 + Synpcc7942_1010 0.71 +3.0 1,022,494 - Synpcc7942_1010 0.72 -0.1 1,022,663 + +2.7 1,022,685 + +2.5 1,022,704 - -1.7 1,022,706 + +2.0 1,022,706 + +0.7 1,022,714 - +4.0 1,022,714 - +2.2 1,022,716 - +1.6 1,022,729 + -0.1 1,022,729 + -0.1 1,022,729 + -0.1 1,022,737 - -0.1 1,022,754 + +2.2 1,022,754 + +0.9 1,022,755 - +4.0 1,022,755 - +0.9 1,022,760 - +2.9 1,022,777 + -1.7 1,022,777 + -1.1 1,022,785 - -0.1 1,022,873 - +1.2 1,022,892 + sphS Synpcc7942_1011 0.14 -1.7 1,022,892 + sphS Synpcc7942_1011 0.14 +2.9 1,022,919 - sphS Synpcc7942_1011 0.16 +1.7 1,022,920 + sphS Synpcc7942_1011 0.17 -0.7 1,022,943 - sphS Synpcc7942_1011 0.18 -1.1 1,022,943 - sphS Synpcc7942_1011 0.18 +0.6 1,022,978 + sphS Synpcc7942_1011 0.21 -0.1 1,022,978 + sphS Synpcc7942_1011 0.21 +3.2 1,022,981 - sphS Synpcc7942_1011 0.21 +2.0 1,022,981 - sphS Synpcc7942_1011 0.21 +1.3 1,022,986 - sphS Synpcc7942_1011 0.22 +2.7 1,022,991 + sphS Synpcc7942_1011 0.22 -0.9 1,023,006 + sphS Synpcc7942_1011 0.23 +0.5 1,023,014 - sphS Synpcc7942_1011 0.24 +1.8 1,023,014 - sphS Synpcc7942_1011 0.24 +2.7 1,023,026 + -0.1 1,023,026 + sphS Synpcc7942_1011 0.25 +2.9 1,023,032 + sphS Synpcc7942_1011 0.26 +1.0 1,023,032 + sphS Synpcc7942_1011 0.26 -0.1 1,023,040 - sphS Synpcc7942_1011 0.26 +0.9 1,023,040 - sphS Synpcc7942_1011 0.26 -1.1 1,023,045 - sphS Synpcc7942_1011 0.27 +0.9 1,023,133 - sphS Synpcc7942_1011 0.34 -0.1 1,023,138 - -1.1 1,023,140 - sphS Synpcc7942_1011 0.34 -0.1 1,023,145 - sphS Synpcc7942_1011 0.35 +4.2 1,023,154 + sphS Synpcc7942_1011 0.35 +1.4 1,023,180 - sphS Synpcc7942_1011 0.37 +0.6 1,023,185 + sphS Synpcc7942_1011 0.38 -1.1 1,023,185 + sphS Synpcc7942_1011 0.38 +3.4 1,023,185 + -0.1 1,023,185 + sphS Synpcc7942_1011 0.38 +0.7 1,023,193 - sphS Synpcc7942_1011 0.38 -0.1 1,023,193 - sphS Synpcc7942_1011 0.38 -1.7 1,023,193 - sphS Synpcc7942_1011 0.38 +2.6 1,023,201 - sphS Synpcc7942_1011 0.39 +3.7 1,023,223 - sphS Synpcc7942_1011 0.41 +2.7 1,023,225 + sphS Synpcc7942_1011 0.41 +2.4 1,023,261 - sphS Synpcc7942_1011 0.44 +3.7 1,023,272 + sphS Synpcc7942_1011 0.45 -0.7 1,023,273 + sphS Synpcc7942_1011 0.45 +2.8 1,023,281 - sphS Synpcc7942_1011 0.46 +2.3 1,023,337 + sphS Synpcc7942_1011 0.50 +0.6 1,023,374 + sphS Synpcc7942_1011 0.53 +0.8 1,023,384 - sphS Synpcc7942_1011 0.54 -1.1 1,023,460 + sphS Synpcc7942_1011 0.60 +2.0 1,023,462 + sphS Synpcc7942_1011 0.60 -0.1 1,023,462 + sphS Synpcc7942_1011 0.60 +3.9 1,023,465 - sphS Synpcc7942_1011 0.60 -0.9 1,023,494 - sphS Synpcc7942_1011 0.63 -1.1 1,023,506 - sphS Synpcc7942_1011 0.64 +2.2 1,023,528 + sphS Synpcc7942_1011 0.65 +2.5 1,023,528 + sphS Synpcc7942_1011 0.65 +2.9 1,023,528 + sphS Synpcc7942_1011 0.65 +3.0 1,023,528 + sphS Synpcc7942_1011 0.65 +1.5 1,023,528 + sphS Synpcc7942_1011 0.65 -2.1 1,023,536 - sphS Synpcc7942_1011 0.66 -2.1 1,023,536 - sphS Synpcc7942_1011 0.66 -2.1 1,023,536 - sphS Synpcc7942_1011 0.66 -1.1 1,023,541 - sphS Synpcc7942_1011 0.67 +1.9 1,023,547 - sphS Synpcc7942_1011 0.67 +0.5 1,023,554 + sphS Synpcc7942_1011 0.68 -0.1 1,023,554 + sphS Synpcc7942_1011 0.68 +1.4 1,023,567 - sphS Synpcc7942_1011 0.69 +0.9 1,023,574 + sphS Synpcc7942_1011 0.69 +1.7 1,023,593 - sphS Synpcc7942_1011 0.71 +1.9 1,023,619 - sphS Synpcc7942_1011 0.73 +2.8 1,023,621 - sphS Synpcc7942_1011 0.73 +0.8 1,023,650 + sphS Synpcc7942_1011 0.75 -0.1 1,023,668 + sphS Synpcc7942_1011 0.77 +3.0 1,023,668 + sphS Synpcc7942_1011 0.77 -1.1 1,023,668 + sphS Synpcc7942_1011 0.77 -1.1 1,023,675 - sphS Synpcc7942_1011 0.77 -2.1 1,023,675 - sphS Synpcc7942_1011 0.77 +1.4 1,023,684 + sphS Synpcc7942_1011 0.78 +1.4 1,023,685 + sphS Synpcc7942_1011 0.78 +5.0
Or see this region's nucleotide sequence