Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_665 and Pf1N1B4_666 are separated by 37 nucleotides Pf1N1B4_666 and Pf1N1B4_667 are separated by 170 nucleotides Pf1N1B4_667 and Pf1N1B4_668 are separated by 98 nucleotides Pf1N1B4_668 and Pf1N1B4_669 are separated by 169 nucleotides Pf1N1B4_669 and Pf1N1B4_670 overlap by 4 nucleotides
Pf1N1B4_665 - Formyltetrahydrofolate deformylase (EC 3.5.1.10), at 762,094 to 762,942
_665
Pf1N1B4_666 - hypothetical protein, at 762,980 to 763,099
_666
Pf1N1B4_667 - transcriptional regulator MvaT, P16 subunit, at 763,270 to 763,647
_667
Pf1N1B4_668 - Exodeoxyribonuclease I (EC 3.1.11.1), at 763,746 to 765,176
_668
Pf1N1B4_669 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 765,346 to 766,071
_669
Pf1N1B4_670 - FIG01248689: hypothetical protein, at 766,068 to 767,045
_670
Position (kb) 763
764
765
766 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 763.017 kb on + strand at 763.115 kb on + strand at 763.115 kb on + strand at 763.131 kb on + strand at 763.131 kb on + strand at 763.166 kb on + strand at 763.175 kb on + strand at 763.257 kb on + strand at 763.308 kb on + strand, within Pf1N1B4_667 at 763.736 kb on - strand at 763.821 kb on - strand at 763.821 kb on - strand at 763.897 kb on - strand, within Pf1N1B4_668 at 763.897 kb on - strand, within Pf1N1B4_668 at 763.897 kb on - strand at 763.984 kb on - strand, within Pf1N1B4_668 at 764.029 kb on - strand, within Pf1N1B4_668 at 764.101 kb on - strand at 764.162 kb on + strand, within Pf1N1B4_668 at 764.247 kb on + strand at 764.248 kb on - strand, within Pf1N1B4_668 at 764.292 kb on + strand, within Pf1N1B4_668 at 764.293 kb on - strand at 764.293 kb on - strand, within Pf1N1B4_668 at 764.293 kb on - strand, within Pf1N1B4_668 at 764.479 kb on + strand, within Pf1N1B4_668 at 764.479 kb on + strand, within Pf1N1B4_668 at 764.479 kb on + strand, within Pf1N1B4_668 at 764.480 kb on - strand, within Pf1N1B4_668 at 764.480 kb on - strand, within Pf1N1B4_668 at 764.480 kb on - strand, within Pf1N1B4_668 at 764.480 kb on - strand, within Pf1N1B4_668 at 764.480 kb on - strand, within Pf1N1B4_668 at 764.480 kb on - strand, within Pf1N1B4_668 at 764.480 kb on - strand, within Pf1N1B4_668 at 764.511 kb on - strand, within Pf1N1B4_668 at 764.585 kb on - strand, within Pf1N1B4_668 at 764.585 kb on - strand, within Pf1N1B4_668 at 764.585 kb on - strand, within Pf1N1B4_668 at 764.586 kb on + strand, within Pf1N1B4_668 at 764.586 kb on + strand, within Pf1N1B4_668 at 764.586 kb on + strand at 764.587 kb on - strand, within Pf1N1B4_668 at 764.587 kb on - strand, within Pf1N1B4_668 at 764.587 kb on - strand, within Pf1N1B4_668 at 764.628 kb on - strand, within Pf1N1B4_668 at 764.765 kb on - strand, within Pf1N1B4_668 at 764.765 kb on - strand, within Pf1N1B4_668 at 764.827 kb on + strand, within Pf1N1B4_668 at 764.991 kb on - strand at 765.049 kb on + strand at 765.070 kb on - strand at 765.151 kb on + strand at 765.152 kb on - strand at 765.158 kb on - strand at 765.209 kb on + strand at 765.210 kb on - strand at 765.219 kb on + strand at 765.219 kb on + strand at 765.219 kb on + strand at 765.219 kb on + strand at 765.219 kb on + strand at 765.219 kb on + strand at 765.219 kb on + strand at 765.219 kb on + strand at 765.220 kb on - strand at 765.220 kb on - strand at 765.220 kb on - strand at 765.220 kb on - strand at 765.220 kb on - strand at 765.276 kb on + strand at 765.276 kb on + strand at 765.276 kb on + strand at 765.276 kb on + strand at 765.276 kb on + strand at 765.276 kb on + strand at 765.277 kb on - strand at 765.277 kb on - strand at 765.277 kb on - strand at 765.277 kb on - strand at 765.277 kb on - strand at 765.277 kb on - strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.280 kb on + strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.281 kb on - strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.284 kb on + strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.285 kb on - strand at 765.311 kb on - strand at 765.598 kb on + strand at 765.598 kb on + strand, within Pf1N1B4_669 at 765.598 kb on + strand, within Pf1N1B4_669 at 765.598 kb on + strand, within Pf1N1B4_669 at 765.598 kb on + strand, within Pf1N1B4_669 at 765.598 kb on + strand, within Pf1N1B4_669 at 765.599 kb on - strand, within Pf1N1B4_669 at 765.599 kb on - strand, within Pf1N1B4_669 at 765.599 kb on - strand, within Pf1N1B4_669 at 765.599 kb on - strand, within Pf1N1B4_669 at 765.599 kb on - strand, within Pf1N1B4_669 at 765.751 kb on + strand, within Pf1N1B4_669 at 765.987 kb on - strand, within Pf1N1B4_669
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 763,017 + -0.3 763,115 + +1.3 763,115 + +0.8 763,131 + +0.2 763,131 + +0.0 763,166 + +0.4 763,175 + +2.3 763,257 + +1.7 763,308 + Pf1N1B4_667 0.10 -1.8 763,736 - -1.6 763,821 - -1.1 763,821 - +0.4 763,897 - Pf1N1B4_668 0.11 -1.3 763,897 - Pf1N1B4_668 0.11 -0.6 763,897 - -0.0 763,984 - Pf1N1B4_668 0.17 -0.9 764,029 - Pf1N1B4_668 0.20 -1.9 764,101 - +1.6 764,162 + Pf1N1B4_668 0.29 -0.0 764,247 + +2.0 764,248 - Pf1N1B4_668 0.35 -1.6 764,292 + Pf1N1B4_668 0.38 +1.3 764,293 - +1.0 764,293 - Pf1N1B4_668 0.38 -0.4 764,293 - Pf1N1B4_668 0.38 -0.8 764,479 + Pf1N1B4_668 0.51 -0.0 764,479 + Pf1N1B4_668 0.51 +0.2 764,479 + Pf1N1B4_668 0.51 -0.2 764,480 - Pf1N1B4_668 0.51 -2.1 764,480 - Pf1N1B4_668 0.51 -0.2 764,480 - Pf1N1B4_668 0.51 -1.3 764,480 - Pf1N1B4_668 0.51 -0.3 764,480 - Pf1N1B4_668 0.51 +1.0 764,480 - Pf1N1B4_668 0.51 +0.2 764,480 - Pf1N1B4_668 0.51 -1.8 764,511 - Pf1N1B4_668 0.53 -1.3 764,585 - Pf1N1B4_668 0.59 -2.6 764,585 - Pf1N1B4_668 0.59 -0.9 764,585 - Pf1N1B4_668 0.59 -0.6 764,586 + Pf1N1B4_668 0.59 +0.4 764,586 + Pf1N1B4_668 0.59 +1.0 764,586 + +0.4 764,587 - Pf1N1B4_668 0.59 -0.7 764,587 - Pf1N1B4_668 0.59 +1.0 764,587 - Pf1N1B4_668 0.59 -0.8 764,628 - Pf1N1B4_668 0.62 -0.0 764,765 - Pf1N1B4_668 0.71 -0.3 764,765 - Pf1N1B4_668 0.71 -1.3 764,827 + Pf1N1B4_668 0.76 +1.3 764,991 - +0.4 765,049 + +1.0 765,070 - +2.0 765,151 + -2.2 765,152 - +0.2 765,158 - +0.4 765,209 + -0.2 765,210 - -1.3 765,219 + -1.0 765,219 + -0.3 765,219 + -1.3 765,219 + -0.2 765,219 + +0.4 765,219 + +2.0 765,219 + +1.8 765,219 + -0.6 765,220 - -1.3 765,220 - +0.5 765,220 - -0.3 765,220 - -0.1 765,220 - -0.9 765,276 + +1.2 765,276 + -1.8 765,276 + -1.0 765,276 + +0.2 765,276 + -2.5 765,276 + +1.0 765,277 - -0.8 765,277 - -1.0 765,277 - -0.3 765,277 - -0.9 765,277 - +0.2 765,277 - -2.0 765,280 + -1.8 765,280 + +1.3 765,280 + -0.1 765,280 + +1.0 765,280 + -0.6 765,280 + +0.2 765,280 + -1.5 765,280 + -0.3 765,280 + -0.6 765,280 + +1.0 765,280 + +1.0 765,280 + -1.3 765,280 + +3.3 765,280 + +0.2 765,280 + -0.2 765,280 + +0.8 765,280 + -0.0 765,280 + +1.7 765,280 + -1.0 765,281 - +0.8 765,281 - -2.7 765,281 - +1.6 765,281 - -1.0 765,281 - -0.6 765,281 - -0.3 765,281 - -0.3 765,281 - -0.2 765,281 - +0.6 765,281 - +1.0 765,281 - -1.2 765,281 - -0.0 765,281 - -1.1 765,281 - -1.3 765,281 - +1.0 765,281 - -0.2 765,281 - -0.0 765,281 - -0.0 765,281 - +0.4 765,281 - -0.4 765,281 - +1.4 765,284 + +2.6 765,284 + -0.6 765,284 + +0.7 765,284 + +0.3 765,284 + +0.3 765,284 + -0.6 765,284 + -0.0 765,284 + -0.0 765,284 + -1.6 765,284 + -1.3 765,284 + +1.0 765,284 + -1.0 765,284 + -1.0 765,284 + -1.8 765,284 + -1.0 765,284 + -1.3 765,284 + -0.2 765,285 - -0.3 765,285 - +1.5 765,285 - +2.0 765,285 - +0.8 765,285 - +2.1 765,285 - -0.2 765,285 - +1.2 765,285 - -0.6 765,285 - -0.6 765,285 - -2.3 765,285 - -1.8 765,285 - +1.2 765,285 - +0.3 765,285 - +0.6 765,285 - +1.0 765,285 - +1.0 765,311 - +1.7 765,598 + +1.0 765,598 + Pf1N1B4_669 0.35 +1.3 765,598 + Pf1N1B4_669 0.35 -1.6 765,598 + Pf1N1B4_669 0.35 +1.0 765,598 + Pf1N1B4_669 0.35 +0.2 765,598 + Pf1N1B4_669 0.35 -0.0 765,599 - Pf1N1B4_669 0.35 -0.4 765,599 - Pf1N1B4_669 0.35 -0.2 765,599 - Pf1N1B4_669 0.35 +1.5 765,599 - Pf1N1B4_669 0.35 +4.0 765,599 - Pf1N1B4_669 0.35 +0.8 765,751 + Pf1N1B4_669 0.56 -0.6 765,987 - Pf1N1B4_669 0.88 -0.0
Or see this region's nucleotide sequence