Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_546 and Pf1N1B4_547 are separated by 229 nucleotides Pf1N1B4_547 and Pf1N1B4_548 are separated by 49 nucleotides Pf1N1B4_548 and Pf1N1B4_549 are separated by 14 nucleotides
Pf1N1B4_546 - FMN-dependent NADH-azoreductase, at 644,148 to 644,747
_546
Pf1N1B4_547 - 3-phosphoglycerate kinase, at 644,977 to 645,306
_547
Pf1N1B4_548 - Transcriptional regulator, LysR family, at 645,356 to 646,279
_548
Pf1N1B4_549 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 646,294 to 647,097
_549
Position (kb) 644
645
646 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 643.977 kb on - strand at 643.977 kb on - strand at 643.977 kb on - strand at 644.115 kb on - strand at 644.115 kb on - strand at 644.130 kb on + strand at 644.131 kb on - strand at 644.281 kb on + strand, within Pf1N1B4_546 at 644.281 kb on + strand, within Pf1N1B4_546 at 644.281 kb on + strand, within Pf1N1B4_546 at 644.281 kb on + strand, within Pf1N1B4_546 at 644.282 kb on - strand, within Pf1N1B4_546 at 644.282 kb on - strand, within Pf1N1B4_546 at 644.282 kb on - strand, within Pf1N1B4_546 at 644.282 kb on - strand, within Pf1N1B4_546 at 644.314 kb on + strand, within Pf1N1B4_546 at 644.315 kb on - strand, within Pf1N1B4_546 at 644.315 kb on - strand, within Pf1N1B4_546 at 644.341 kb on + strand, within Pf1N1B4_546 at 644.341 kb on + strand, within Pf1N1B4_546 at 644.342 kb on - strand, within Pf1N1B4_546 at 644.342 kb on - strand, within Pf1N1B4_546 at 644.342 kb on - strand, within Pf1N1B4_546 at 644.386 kb on + strand, within Pf1N1B4_546 at 644.386 kb on + strand, within Pf1N1B4_546 at 644.387 kb on - strand, within Pf1N1B4_546 at 644.387 kb on - strand, within Pf1N1B4_546 at 644.458 kb on + strand, within Pf1N1B4_546 at 644.459 kb on - strand, within Pf1N1B4_546 at 644.459 kb on - strand, within Pf1N1B4_546 at 644.476 kb on - strand, within Pf1N1B4_546 at 644.481 kb on + strand, within Pf1N1B4_546 at 644.481 kb on + strand, within Pf1N1B4_546 at 644.481 kb on + strand, within Pf1N1B4_546 at 644.482 kb on - strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.615 kb on + strand, within Pf1N1B4_546 at 644.616 kb on - strand at 644.616 kb on - strand, within Pf1N1B4_546 at 644.616 kb on - strand, within Pf1N1B4_546 at 644.616 kb on - strand, within Pf1N1B4_546 at 644.616 kb on - strand, within Pf1N1B4_546 at 644.616 kb on - strand, within Pf1N1B4_546 at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.754 kb on + strand at 644.755 kb on - strand at 644.755 kb on - strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.776 kb on + strand at 644.777 kb on - strand at 644.777 kb on - strand at 644.777 kb on - strand at 644.828 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.972 kb on + strand at 644.973 kb on - strand at 644.973 kb on - strand at 645.003 kb on + strand at 645.004 kb on - strand at 645.140 kb on + strand, within Pf1N1B4_547 at 645.177 kb on + strand, within Pf1N1B4_547 at 645.177 kb on + strand at 645.177 kb on + strand, within Pf1N1B4_547 at 645.178 kb on - strand, within Pf1N1B4_547 at 645.178 kb on - strand, within Pf1N1B4_547 at 645.191 kb on + strand, within Pf1N1B4_547 at 645.192 kb on - strand, within Pf1N1B4_547 at 645.237 kb on - strand, within Pf1N1B4_547 at 645.291 kb on + strand at 645.291 kb on + strand at 645.292 kb on - strand at 645.292 kb on - strand at 645.292 kb on - strand at 645.292 kb on - strand at 645.337 kb on - strand at 645.337 kb on - strand at 645.337 kb on - strand at 645.397 kb on - strand at 645.397 kb on - strand at 645.397 kb on - strand at 645.397 kb on - strand at 645.470 kb on + strand, within Pf1N1B4_548 at 645.470 kb on + strand, within Pf1N1B4_548 at 645.471 kb on - strand at 645.471 kb on - strand, within Pf1N1B4_548 at 645.513 kb on + strand, within Pf1N1B4_548 at 646.122 kb on + strand, within Pf1N1B4_548 at 646.160 kb on + strand, within Pf1N1B4_548 at 646.160 kb on + strand, within Pf1N1B4_548 at 646.160 kb on + strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.161 kb on - strand, within Pf1N1B4_548 at 646.166 kb on + strand, within Pf1N1B4_548 at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.289 kb on + strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.290 kb on - strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.293 kb on + strand at 646.294 kb on - strand at 646.294 kb on - strand at 646.294 kb on - strand at 646.294 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 643,977 - -0.0 643,977 - -1.0 643,977 - +1.0 644,115 - +0.5 644,115 - -1.6 644,130 + -0.4 644,131 - -0.4 644,281 + Pf1N1B4_546 0.22 +0.2 644,281 + Pf1N1B4_546 0.22 -1.9 644,281 + Pf1N1B4_546 0.22 -0.4 644,281 + Pf1N1B4_546 0.22 -1.0 644,282 - Pf1N1B4_546 0.22 -0.2 644,282 - Pf1N1B4_546 0.22 -1.9 644,282 - Pf1N1B4_546 0.22 +0.4 644,282 - Pf1N1B4_546 0.22 -1.2 644,314 + Pf1N1B4_546 0.28 -1.6 644,315 - Pf1N1B4_546 0.28 +1.8 644,315 - Pf1N1B4_546 0.28 -1.6 644,341 + Pf1N1B4_546 0.32 +0.3 644,341 + Pf1N1B4_546 0.32 -2.6 644,342 - Pf1N1B4_546 0.32 +0.5 644,342 - Pf1N1B4_546 0.32 -0.0 644,342 - Pf1N1B4_546 0.32 +0.6 644,386 + Pf1N1B4_546 0.40 -0.5 644,386 + Pf1N1B4_546 0.40 +0.1 644,387 - Pf1N1B4_546 0.40 +1.0 644,387 - Pf1N1B4_546 0.40 -0.4 644,458 + Pf1N1B4_546 0.52 -1.1 644,459 - Pf1N1B4_546 0.52 +1.4 644,459 - Pf1N1B4_546 0.52 -0.0 644,476 - Pf1N1B4_546 0.55 -0.7 644,481 + Pf1N1B4_546 0.56 -0.9 644,481 + Pf1N1B4_546 0.56 +1.4 644,481 + Pf1N1B4_546 0.56 -1.9 644,482 - Pf1N1B4_546 0.56 -0.5 644,615 + Pf1N1B4_546 0.78 +0.1 644,615 + Pf1N1B4_546 0.78 -0.8 644,615 + Pf1N1B4_546 0.78 +1.0 644,615 + Pf1N1B4_546 0.78 -1.9 644,615 + Pf1N1B4_546 0.78 -0.3 644,615 + Pf1N1B4_546 0.78 -1.2 644,615 + Pf1N1B4_546 0.78 -1.6 644,615 + Pf1N1B4_546 0.78 -0.0 644,616 - +0.5 644,616 - Pf1N1B4_546 0.78 -0.6 644,616 - Pf1N1B4_546 0.78 +0.3 644,616 - Pf1N1B4_546 0.78 +0.5 644,616 - Pf1N1B4_546 0.78 -1.1 644,616 - Pf1N1B4_546 0.78 +1.3 644,754 + +0.5 644,754 + -1.0 644,754 + -0.9 644,754 + +0.4 644,754 + +0.4 644,754 + -0.1 644,754 + +0.0 644,754 + +0.8 644,754 + +0.2 644,755 - +0.7 644,755 - +1.5 644,776 + +1.4 644,776 + -0.6 644,776 + -1.7 644,776 + -0.6 644,776 + -1.6 644,776 + -1.4 644,777 - +2.5 644,777 - -0.6 644,777 - -0.0 644,828 + -1.2 644,972 + -2.0 644,972 + -0.6 644,972 + -2.0 644,972 + -1.2 644,972 + +0.5 644,972 + -0.0 644,972 + -1.2 644,973 - +0.4 644,973 - -0.4 645,003 + +0.6 645,004 - -0.6 645,140 + Pf1N1B4_547 0.49 -0.8 645,177 + Pf1N1B4_547 0.61 -0.7 645,177 + -0.6 645,177 + Pf1N1B4_547 0.61 -0.6 645,178 - Pf1N1B4_547 0.61 -1.4 645,178 - Pf1N1B4_547 0.61 -1.7 645,191 + Pf1N1B4_547 0.65 -1.1 645,192 - Pf1N1B4_547 0.65 -0.3 645,237 - Pf1N1B4_547 0.79 +1.6 645,291 + -1.2 645,291 + +0.2 645,292 - -0.4 645,292 - -0.0 645,292 - +1.1 645,292 - -0.6 645,337 - -1.6 645,337 - -1.0 645,337 - +0.3 645,397 - -0.0 645,397 - +1.1 645,397 - -0.6 645,397 - +0.6 645,470 + Pf1N1B4_548 0.12 -1.0 645,470 + Pf1N1B4_548 0.12 -1.0 645,471 - -0.6 645,471 - Pf1N1B4_548 0.12 +0.0 645,513 + Pf1N1B4_548 0.17 -0.6 646,122 + Pf1N1B4_548 0.83 +4.0 646,160 + Pf1N1B4_548 0.87 -1.0 646,160 + Pf1N1B4_548 0.87 -1.0 646,160 + Pf1N1B4_548 0.87 +0.1 646,161 - Pf1N1B4_548 0.87 -1.6 646,161 - Pf1N1B4_548 0.87 -1.4 646,161 - Pf1N1B4_548 0.87 +0.2 646,161 - Pf1N1B4_548 0.87 -1.0 646,161 - Pf1N1B4_548 0.87 +0.1 646,161 - Pf1N1B4_548 0.87 +1.2 646,161 - Pf1N1B4_548 0.87 +1.7 646,166 + Pf1N1B4_548 0.88 +1.2 646,289 + +0.4 646,289 + -0.6 646,289 + +0.3 646,289 + +0.1 646,289 + -0.6 646,289 + +0.4 646,289 + -1.6 646,289 + +1.4 646,289 + -2.1 646,289 + +1.3 646,289 + -0.6 646,289 + -1.4 646,290 - -1.2 646,290 - +1.0 646,290 - +0.7 646,290 - +1.0 646,290 - +0.1 646,290 - +0.4 646,290 - +0.1 646,290 - +0.7 646,290 - +1.0 646,290 - +2.0 646,290 - -0.6 646,290 - +1.2 646,290 - -2.0 646,290 - -0.6 646,293 + +0.6 646,293 + -0.0 646,293 + +1.0 646,293 + +1.0 646,293 + -1.3 646,293 + -0.9 646,293 + -1.2 646,294 - +1.1 646,294 - -1.0 646,294 - -0.0 646,294 - +1.0
Or see this region's nucleotide sequence