Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5094 and Pf1N1B4_5095 are separated by 80 nucleotides Pf1N1B4_5095 and Pf1N1B4_5096 are separated by 357 nucleotides
Pf1N1B4_5094 - Methyl-accepting chemotaxis protein, at 5,647,912 to 5,648,736
_5094
Pf1N1B4_5095 - hypothetical protein, at 5,648,817 to 5,649,380
_5095
Pf1N1B4_5096 - Nitrate ABC transporter, nitrate-binding protein, at 5,649,738 to 5,651,024
_5096
Position (kb)
5648
5649
5650 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5647.826 kb on + strand at 5647.827 kb on - strand at 5647.827 kb on - strand at 5647.833 kb on - strand at 5647.949 kb on + strand at 5648.236 kb on - strand, within Pf1N1B4_5094 at 5648.547 kb on + strand, within Pf1N1B4_5094 at 5648.548 kb on - strand, within Pf1N1B4_5094 at 5648.548 kb on - strand, within Pf1N1B4_5094 at 5648.667 kb on + strand at 5648.741 kb on - strand at 5648.952 kb on + strand, within Pf1N1B4_5095 at 5648.984 kb on - strand, within Pf1N1B4_5095 at 5649.200 kb on - strand, within Pf1N1B4_5095 at 5649.200 kb on - strand, within Pf1N1B4_5095 at 5649.205 kb on - strand, within Pf1N1B4_5095 at 5649.385 kb on + strand at 5649.385 kb on + strand at 5649.385 kb on + strand at 5649.386 kb on - strand at 5649.386 kb on - strand at 5649.427 kb on + strand at 5649.487 kb on + strand at 5649.959 kb on - strand, within Pf1N1B4_5096 at 5649.991 kb on - strand at 5650.095 kb on + strand, within Pf1N1B4_5096 at 5650.095 kb on + strand, within Pf1N1B4_5096 at 5650.096 kb on - strand, within Pf1N1B4_5096 at 5650.309 kb on - strand, within Pf1N1B4_5096
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 5,647,826 + +1.5 5,647,827 - -1.1 5,647,827 - -0.4 5,647,833 - +1.2 5,647,949 + -0.5 5,648,236 - Pf1N1B4_5094 0.39 -0.8 5,648,547 + Pf1N1B4_5094 0.77 -3.1 5,648,548 - Pf1N1B4_5094 0.77 -0.8 5,648,548 - Pf1N1B4_5094 0.77 +0.1 5,648,667 + -0.5 5,648,741 - +2.8 5,648,952 + Pf1N1B4_5095 0.24 +0.3 5,648,984 - Pf1N1B4_5095 0.30 -0.4 5,649,200 - Pf1N1B4_5095 0.68 -1.8 5,649,200 - Pf1N1B4_5095 0.68 -2.3 5,649,205 - Pf1N1B4_5095 0.69 -1.6 5,649,385 + -0.1 5,649,385 + -0.5 5,649,385 + -1.1 5,649,386 - -1.2 5,649,386 - -0.5 5,649,427 + +0.2 5,649,487 + -0.3 5,649,959 - Pf1N1B4_5096 0.17 -0.6 5,649,991 - -1.1 5,650,095 + Pf1N1B4_5096 0.28 -0.7 5,650,095 + Pf1N1B4_5096 0.28 -1.5 5,650,096 - Pf1N1B4_5096 0.28 +0.3 5,650,309 - Pf1N1B4_5096 0.44 +0.2
Or see this region's nucleotide sequence