Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5009 and Pf1N1B4_5010 are separated by 185 nucleotides Pf1N1B4_5010 and Pf1N1B4_5011 are separated by 125 nucleotides Pf1N1B4_5011 and Pf1N1B4_5012 are separated by 137 nucleotides
Pf1N1B4_5009 - Lactoylglutathione lyase (EC 4.4.1.5), at 5,559,879 to 5,560,400
_5009
Pf1N1B4_5010 - Alkyl hydroperoxide reductase protein F (EC 1.6.4.-), at 5,560,586 to 5,562,148
_5010
Pf1N1B4_5011 - Alkyl hydroperoxide reductase protein C (EC 1.6.4.-), at 5,562,274 to 5,562,837
_5011
Pf1N1B4_5012 - Cointegrate resolution protein S, at 5,562,975 to 5,563,970
_5012
Position (kb)
5560
5561
5562
5563 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5559.680 kb on - strand at 5559.841 kb on + strand at 5559.842 kb on - strand at 5559.899 kb on + strand at 5560.068 kb on + strand at 5560.238 kb on + strand, within Pf1N1B4_5009 at 5560.398 kb on + strand at 5560.398 kb on + strand at 5560.441 kb on + strand at 5560.587 kb on + strand at 5560.673 kb on - strand at 5560.673 kb on - strand at 5560.937 kb on - strand, within Pf1N1B4_5010 at 5560.992 kb on - strand at 5561.124 kb on + strand, within Pf1N1B4_5010 at 5561.141 kb on - strand, within Pf1N1B4_5010 at 5561.453 kb on - strand, within Pf1N1B4_5010 at 5561.463 kb on + strand, within Pf1N1B4_5010 at 5561.464 kb on - strand, within Pf1N1B4_5010 at 5561.508 kb on - strand, within Pf1N1B4_5010 at 5561.508 kb on - strand, within Pf1N1B4_5010 at 5561.628 kb on + strand, within Pf1N1B4_5010 at 5561.629 kb on - strand, within Pf1N1B4_5010 at 5561.781 kb on + strand, within Pf1N1B4_5010 at 5561.782 kb on - strand at 5561.782 kb on - strand, within Pf1N1B4_5010 at 5561.782 kb on - strand, within Pf1N1B4_5010 at 5561.782 kb on - strand, within Pf1N1B4_5010 at 5561.998 kb on + strand at 5561.998 kb on + strand at 5562.148 kb on + strand at 5562.148 kb on + strand at 5562.148 kb on + strand at 5562.229 kb on - strand at 5562.229 kb on - strand at 5562.232 kb on + strand at 5562.232 kb on + strand at 5562.276 kb on - strand at 5562.276 kb on - strand at 5562.276 kb on - strand at 5562.416 kb on - strand, within Pf1N1B4_5011 at 5562.813 kb on + strand at 5562.882 kb on - strand at 5562.956 kb on + strand at 5562.960 kb on - strand at 5563.036 kb on + strand at 5563.037 kb on - strand at 5563.037 kb on - strand at 5563.037 kb on - strand at 5563.072 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 5,559,680 - +1.1 5,559,841 + -1.5 5,559,842 - +0.5 5,559,899 + +2.8 5,560,068 + -1.1 5,560,238 + Pf1N1B4_5009 0.69 -0.1 5,560,398 + +1.1 5,560,398 + -0.2 5,560,441 + -2.0 5,560,587 + -1.2 5,560,673 - -1.1 5,560,673 - +0.1 5,560,937 - Pf1N1B4_5010 0.22 -1.0 5,560,992 - -1.1 5,561,124 + Pf1N1B4_5010 0.34 -1.5 5,561,141 - Pf1N1B4_5010 0.36 -1.3 5,561,453 - Pf1N1B4_5010 0.55 +0.2 5,561,463 + Pf1N1B4_5010 0.56 +0.7 5,561,464 - Pf1N1B4_5010 0.56 -1.1 5,561,508 - Pf1N1B4_5010 0.59 -3.0 5,561,508 - Pf1N1B4_5010 0.59 -1.1 5,561,628 + Pf1N1B4_5010 0.67 -1.1 5,561,629 - Pf1N1B4_5010 0.67 -0.1 5,561,781 + Pf1N1B4_5010 0.76 -3.1 5,561,782 - -0.5 5,561,782 - Pf1N1B4_5010 0.77 -1.1 5,561,782 - Pf1N1B4_5010 0.77 -2.3 5,561,782 - Pf1N1B4_5010 0.77 +0.5 5,561,998 + -0.1 5,561,998 + -1.4 5,562,148 + +1.1 5,562,148 + -1.7 5,562,148 + +0.8 5,562,229 - -1.5 5,562,229 - -0.3 5,562,232 + +1.5 5,562,232 + -1.5 5,562,276 - -1.7 5,562,276 - +0.5 5,562,276 - -1.2 5,562,416 - Pf1N1B4_5011 0.25 +0.3 5,562,813 + +0.2 5,562,882 - -0.9 5,562,956 + +0.9 5,562,960 - -0.5 5,563,036 + -1.1 5,563,037 - -1.1 5,563,037 - -1.8 5,563,037 - -1.1 5,563,072 + -1.1
Or see this region's nucleotide sequence