Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5010

Experiment: Choline chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5009 and Pf1N1B4_5010 are separated by 185 nucleotidesPf1N1B4_5010 and Pf1N1B4_5011 are separated by 125 nucleotidesPf1N1B4_5011 and Pf1N1B4_5012 are separated by 137 nucleotides Pf1N1B4_5009 - Lactoylglutathione lyase (EC 4.4.1.5), at 5,559,879 to 5,560,400 _5009 Pf1N1B4_5010 - Alkyl hydroperoxide reductase protein F (EC 1.6.4.-), at 5,560,586 to 5,562,148 _5010 Pf1N1B4_5011 - Alkyl hydroperoxide reductase protein C (EC 1.6.4.-), at 5,562,274 to 5,562,837 _5011 Pf1N1B4_5012 - Cointegrate resolution protein S, at 5,562,975 to 5,563,970 _5012 Position (kb) 5560 5561 5562 5563Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 5559.680 kb on - strandat 5559.841 kb on + strandat 5559.842 kb on - strandat 5559.899 kb on + strandat 5560.068 kb on + strandat 5560.238 kb on + strand, within Pf1N1B4_5009at 5560.398 kb on + strandat 5560.398 kb on + strandat 5560.441 kb on + strandat 5560.587 kb on + strandat 5560.673 kb on - strandat 5560.673 kb on - strandat 5560.937 kb on - strand, within Pf1N1B4_5010at 5560.992 kb on - strandat 5561.124 kb on + strand, within Pf1N1B4_5010at 5561.141 kb on - strand, within Pf1N1B4_5010at 5561.453 kb on - strand, within Pf1N1B4_5010at 5561.463 kb on + strand, within Pf1N1B4_5010at 5561.464 kb on - strand, within Pf1N1B4_5010at 5561.508 kb on - strand, within Pf1N1B4_5010at 5561.508 kb on - strand, within Pf1N1B4_5010at 5561.628 kb on + strand, within Pf1N1B4_5010at 5561.629 kb on - strand, within Pf1N1B4_5010at 5561.781 kb on + strand, within Pf1N1B4_5010at 5561.782 kb on - strandat 5561.782 kb on - strand, within Pf1N1B4_5010at 5561.782 kb on - strand, within Pf1N1B4_5010at 5561.782 kb on - strand, within Pf1N1B4_5010at 5561.998 kb on + strandat 5561.998 kb on + strandat 5562.148 kb on + strandat 5562.148 kb on + strandat 5562.148 kb on + strandat 5562.229 kb on - strandat 5562.229 kb on - strandat 5562.232 kb on + strandat 5562.232 kb on + strandat 5562.276 kb on - strandat 5562.276 kb on - strandat 5562.276 kb on - strandat 5562.416 kb on - strand, within Pf1N1B4_5011at 5562.813 kb on + strandat 5562.882 kb on - strandat 5562.956 kb on + strandat 5562.960 kb on - strandat 5563.036 kb on + strandat 5563.037 kb on - strandat 5563.037 kb on - strandat 5563.037 kb on - strandat 5563.072 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline chloride 200 mM
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5,559,680 - +1.1
5,559,841 + -1.5
5,559,842 - +0.5
5,559,899 + +2.8
5,560,068 + -1.1
5,560,238 + Pf1N1B4_5009 0.69 -0.1
5,560,398 + +1.1
5,560,398 + -0.2
5,560,441 + -2.0
5,560,587 + -1.2
5,560,673 - -1.1
5,560,673 - +0.1
5,560,937 - Pf1N1B4_5010 0.22 -1.0
5,560,992 - -1.1
5,561,124 + Pf1N1B4_5010 0.34 -1.5
5,561,141 - Pf1N1B4_5010 0.36 -1.3
5,561,453 - Pf1N1B4_5010 0.55 +0.2
5,561,463 + Pf1N1B4_5010 0.56 +0.7
5,561,464 - Pf1N1B4_5010 0.56 -1.1
5,561,508 - Pf1N1B4_5010 0.59 -3.0
5,561,508 - Pf1N1B4_5010 0.59 -1.1
5,561,628 + Pf1N1B4_5010 0.67 -1.1
5,561,629 - Pf1N1B4_5010 0.67 -0.1
5,561,781 + Pf1N1B4_5010 0.76 -3.1
5,561,782 - -0.5
5,561,782 - Pf1N1B4_5010 0.77 -1.1
5,561,782 - Pf1N1B4_5010 0.77 -2.3
5,561,782 - Pf1N1B4_5010 0.77 +0.5
5,561,998 + -0.1
5,561,998 + -1.4
5,562,148 + +1.1
5,562,148 + -1.7
5,562,148 + +0.8
5,562,229 - -1.5
5,562,229 - -0.3
5,562,232 + +1.5
5,562,232 + -1.5
5,562,276 - -1.7
5,562,276 - +0.5
5,562,276 - -1.2
5,562,416 - Pf1N1B4_5011 0.25 +0.3
5,562,813 + +0.2
5,562,882 - -0.9
5,562,956 + +0.9
5,562,960 - -0.5
5,563,036 + -1.1
5,563,037 - -1.1
5,563,037 - -1.8
5,563,037 - -1.1
5,563,072 + -1.1

Or see this region's nucleotide sequence