Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4764 and Pf1N1B4_4765 are separated by 61 nucleotides Pf1N1B4_4765 and Pf1N1B4_4766 overlap by 8 nucleotides Pf1N1B4_4766 and Pf1N1B4_4767 are separated by 165 nucleotides
Pf1N1B4_4764 - 2-ketogluconate transporter, at 5,260,243 to 5,261,529
_4764
Pf1N1B4_4765 - 2-ketogluconate kinase (EC 2.7.1.13), at 5,261,591 to 5,262,610
_4765
Pf1N1B4_4766 - Epimerase KguE, at 5,262,603 to 5,263,385
_4766
Pf1N1B4_4767 - 2-ketogluconate utilization repressor PtxS, at 5,263,551 to 5,264,576
_4767
Position (kb)
5261
5262
5263 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5260.593 kb on - strand at 5260.593 kb on - strand, within Pf1N1B4_4764 at 5260.700 kb on + strand, within Pf1N1B4_4764 at 5260.981 kb on - strand, within Pf1N1B4_4764 at 5261.315 kb on + strand at 5261.315 kb on + strand at 5261.315 kb on + strand, within Pf1N1B4_4764 at 5261.316 kb on - strand, within Pf1N1B4_4764 at 5261.435 kb on + strand at 5261.436 kb on - strand at 5261.465 kb on + strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.493 kb on - strand at 5261.529 kb on + strand at 5261.908 kb on - strand, within Pf1N1B4_4765 at 5261.908 kb on - strand, within Pf1N1B4_4765 at 5261.908 kb on - strand, within Pf1N1B4_4765 at 5261.962 kb on - strand, within Pf1N1B4_4765 at 5262.291 kb on + strand at 5262.291 kb on + strand, within Pf1N1B4_4765 at 5262.701 kb on - strand, within Pf1N1B4_4766 at 5262.897 kb on - strand, within Pf1N1B4_4766 at 5263.190 kb on - strand at 5263.388 kb on + strand at 5263.389 kb on + strand at 5263.429 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 5,260,593 - +0.5 5,260,593 - Pf1N1B4_4764 0.27 +0.1 5,260,700 + Pf1N1B4_4764 0.36 -0.5 5,260,981 - Pf1N1B4_4764 0.57 -1.9 5,261,315 + -0.1 5,261,315 + -1.1 5,261,315 + Pf1N1B4_4764 0.83 -0.5 5,261,316 - Pf1N1B4_4764 0.83 -2.2 5,261,435 + -1.8 5,261,436 - -0.8 5,261,465 + -0.2 5,261,493 - -0.5 5,261,493 - +1.2 5,261,493 - +1.1 5,261,493 - -1.5 5,261,493 - -0.7 5,261,493 - -0.2 5,261,493 - +2.2 5,261,529 + -0.2 5,261,908 - Pf1N1B4_4765 0.31 -1.5 5,261,908 - Pf1N1B4_4765 0.31 -1.8 5,261,908 - Pf1N1B4_4765 0.31 -0.5 5,261,962 - Pf1N1B4_4765 0.36 -0.6 5,262,291 + +0.5 5,262,291 + Pf1N1B4_4765 0.69 -0.3 5,262,701 - Pf1N1B4_4766 0.13 -0.2 5,262,897 - Pf1N1B4_4766 0.38 +0.1 5,263,190 - +0.1 5,263,388 + +0.5 5,263,389 + -2.7 5,263,429 + +0.5
Or see this region's nucleotide sequence