Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4754

Experiment: Choline chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4753 and Pf1N1B4_4754 are separated by 106 nucleotidesPf1N1B4_4754 and Pf1N1B4_4755 are separated by 201 nucleotides Pf1N1B4_4753 - Soluble aldose sugar dehydrogenase, PQQ-dependent (EC 1.1.5.-), at 5,250,436 to 5,251,587 _4753 Pf1N1B4_4754 - GNAT family acetyltransferase VC2332, at 5,251,694 to 5,252,791 _4754 Pf1N1B4_4755 - ribosomal protein S6 glutaminyl transferase related protein, at 5,252,993 to 5,254,471 _4755 Position (kb) 5251 5252 5253Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5250.772 kb on + strand, within Pf1N1B4_4753at 5250.772 kb on + strand, within Pf1N1B4_4753at 5250.772 kb on + strand, within Pf1N1B4_4753at 5250.772 kb on + strand, within Pf1N1B4_4753at 5250.772 kb on + strand, within Pf1N1B4_4753at 5250.772 kb on + strand, within Pf1N1B4_4753at 5250.773 kb on - strand, within Pf1N1B4_4753at 5250.773 kb on - strand, within Pf1N1B4_4753at 5250.773 kb on - strandat 5250.773 kb on - strand, within Pf1N1B4_4753at 5250.949 kb on + strand, within Pf1N1B4_4753at 5251.255 kb on + strand, within Pf1N1B4_4753at 5251.255 kb on + strand, within Pf1N1B4_4753at 5251.255 kb on + strand, within Pf1N1B4_4753at 5251.255 kb on + strand, within Pf1N1B4_4753at 5251.255 kb on + strand, within Pf1N1B4_4753at 5251.255 kb on + strand, within Pf1N1B4_4753at 5251.508 kb on + strandat 5251.508 kb on + strandat 5251.534 kb on + strandat 5251.534 kb on + strandat 5251.534 kb on + strandat 5251.535 kb on - strandat 5251.535 kb on - strandat 5251.567 kb on + strandat 5251.567 kb on + strandat 5251.842 kb on + strand, within Pf1N1B4_4754at 5251.950 kb on - strand, within Pf1N1B4_4754at 5252.028 kb on - strand, within Pf1N1B4_4754at 5252.028 kb on - strand, within Pf1N1B4_4754at 5252.040 kb on - strand, within Pf1N1B4_4754at 5252.102 kb on + strandat 5252.103 kb on - strand, within Pf1N1B4_4754at 5252.311 kb on - strand, within Pf1N1B4_4754at 5252.392 kb on - strand, within Pf1N1B4_4754at 5252.434 kb on - strandat 5252.578 kb on - strand, within Pf1N1B4_4754at 5252.792 kb on - strandat 5252.885 kb on - strandat 5253.010 kb on + strandat 5253.030 kb on - strandat 5253.132 kb on - strandat 5253.138 kb on - strandat 5253.138 kb on - strandat 5253.153 kb on - strand, within Pf1N1B4_4755at 5253.156 kb on - strand, within Pf1N1B4_4755at 5253.453 kb on + strand, within Pf1N1B4_4755

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline chloride 200 mM
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5,250,772 + Pf1N1B4_4753 0.29 +0.4
5,250,772 + Pf1N1B4_4753 0.29 -1.5
5,250,772 + Pf1N1B4_4753 0.29 +0.1
5,250,772 + Pf1N1B4_4753 0.29 +2.2
5,250,772 + Pf1N1B4_4753 0.29 +0.4
5,250,772 + Pf1N1B4_4753 0.29 -0.5
5,250,773 - Pf1N1B4_4753 0.29 +0.0
5,250,773 - Pf1N1B4_4753 0.29 -0.8
5,250,773 - +0.9
5,250,773 - Pf1N1B4_4753 0.29 +1.5
5,250,949 + Pf1N1B4_4753 0.45 +0.1
5,251,255 + Pf1N1B4_4753 0.71 +0.8
5,251,255 + Pf1N1B4_4753 0.71 +3.4
5,251,255 + Pf1N1B4_4753 0.71 +0.7
5,251,255 + Pf1N1B4_4753 0.71 -0.8
5,251,255 + Pf1N1B4_4753 0.71 +0.5
5,251,255 + Pf1N1B4_4753 0.71 -0.5
5,251,508 + +0.2
5,251,508 + -0.0
5,251,534 + -1.7
5,251,534 + -0.2
5,251,534 + +1.2
5,251,535 - -1.7
5,251,535 - +0.1
5,251,567 + -1.1
5,251,567 + +0.0
5,251,842 + Pf1N1B4_4754 0.13 -1.7
5,251,950 - Pf1N1B4_4754 0.23 -0.1
5,252,028 - Pf1N1B4_4754 0.30 -2.5
5,252,028 - Pf1N1B4_4754 0.30 -2.5
5,252,040 - Pf1N1B4_4754 0.32 -0.7
5,252,102 + +2.1
5,252,103 - Pf1N1B4_4754 0.37 -1.3
5,252,311 - Pf1N1B4_4754 0.56 +0.4
5,252,392 - Pf1N1B4_4754 0.64 -0.8
5,252,434 - +1.5
5,252,578 - Pf1N1B4_4754 0.81 -0.5
5,252,792 - +0.9
5,252,885 - -1.5
5,253,010 + +0.3
5,253,030 - +0.0
5,253,132 - -1.6
5,253,138 - +0.1
5,253,138 - +1.5
5,253,153 - Pf1N1B4_4755 0.11 -1.5
5,253,156 - Pf1N1B4_4755 0.11 -0.5
5,253,453 + Pf1N1B4_4755 0.31 +1.3

Or see this region's nucleotide sequence