Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4753 and Pf1N1B4_4754 are separated by 106 nucleotides Pf1N1B4_4754 and Pf1N1B4_4755 are separated by 201 nucleotides
Pf1N1B4_4753 - Soluble aldose sugar dehydrogenase, PQQ-dependent (EC 1.1.5.-), at 5,250,436 to 5,251,587
_4753
Pf1N1B4_4754 - GNAT family acetyltransferase VC2332, at 5,251,694 to 5,252,791
_4754
Pf1N1B4_4755 - ribosomal protein S6 glutaminyl transferase related protein, at 5,252,993 to 5,254,471
_4755
Position (kb)
5251
5252
5253 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.772 kb on + strand, within Pf1N1B4_4753 at 5250.773 kb on - strand, within Pf1N1B4_4753 at 5250.773 kb on - strand, within Pf1N1B4_4753 at 5250.773 kb on - strand at 5250.773 kb on - strand, within Pf1N1B4_4753 at 5250.949 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.255 kb on + strand, within Pf1N1B4_4753 at 5251.508 kb on + strand at 5251.508 kb on + strand at 5251.534 kb on + strand at 5251.534 kb on + strand at 5251.534 kb on + strand at 5251.535 kb on - strand at 5251.535 kb on - strand at 5251.567 kb on + strand at 5251.567 kb on + strand at 5251.842 kb on + strand, within Pf1N1B4_4754 at 5251.950 kb on - strand, within Pf1N1B4_4754 at 5252.028 kb on - strand, within Pf1N1B4_4754 at 5252.028 kb on - strand, within Pf1N1B4_4754 at 5252.040 kb on - strand, within Pf1N1B4_4754 at 5252.102 kb on + strand at 5252.103 kb on - strand, within Pf1N1B4_4754 at 5252.311 kb on - strand, within Pf1N1B4_4754 at 5252.392 kb on - strand, within Pf1N1B4_4754 at 5252.434 kb on - strand at 5252.578 kb on - strand, within Pf1N1B4_4754 at 5252.792 kb on - strand at 5252.885 kb on - strand at 5253.010 kb on + strand at 5253.030 kb on - strand at 5253.132 kb on - strand at 5253.138 kb on - strand at 5253.138 kb on - strand at 5253.153 kb on - strand, within Pf1N1B4_4755 at 5253.156 kb on - strand, within Pf1N1B4_4755 at 5253.453 kb on + strand, within Pf1N1B4_4755
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 5,250,772 + Pf1N1B4_4753 0.29 +0.4 5,250,772 + Pf1N1B4_4753 0.29 -1.5 5,250,772 + Pf1N1B4_4753 0.29 +0.1 5,250,772 + Pf1N1B4_4753 0.29 +2.2 5,250,772 + Pf1N1B4_4753 0.29 +0.4 5,250,772 + Pf1N1B4_4753 0.29 -0.5 5,250,773 - Pf1N1B4_4753 0.29 +0.0 5,250,773 - Pf1N1B4_4753 0.29 -0.8 5,250,773 - +0.9 5,250,773 - Pf1N1B4_4753 0.29 +1.5 5,250,949 + Pf1N1B4_4753 0.45 +0.1 5,251,255 + Pf1N1B4_4753 0.71 +0.8 5,251,255 + Pf1N1B4_4753 0.71 +3.4 5,251,255 + Pf1N1B4_4753 0.71 +0.7 5,251,255 + Pf1N1B4_4753 0.71 -0.8 5,251,255 + Pf1N1B4_4753 0.71 +0.5 5,251,255 + Pf1N1B4_4753 0.71 -0.5 5,251,508 + +0.2 5,251,508 + -0.0 5,251,534 + -1.7 5,251,534 + -0.2 5,251,534 + +1.2 5,251,535 - -1.7 5,251,535 - +0.1 5,251,567 + -1.1 5,251,567 + +0.0 5,251,842 + Pf1N1B4_4754 0.13 -1.7 5,251,950 - Pf1N1B4_4754 0.23 -0.1 5,252,028 - Pf1N1B4_4754 0.30 -2.5 5,252,028 - Pf1N1B4_4754 0.30 -2.5 5,252,040 - Pf1N1B4_4754 0.32 -0.7 5,252,102 + +2.1 5,252,103 - Pf1N1B4_4754 0.37 -1.3 5,252,311 - Pf1N1B4_4754 0.56 +0.4 5,252,392 - Pf1N1B4_4754 0.64 -0.8 5,252,434 - +1.5 5,252,578 - Pf1N1B4_4754 0.81 -0.5 5,252,792 - +0.9 5,252,885 - -1.5 5,253,010 + +0.3 5,253,030 - +0.0 5,253,132 - -1.6 5,253,138 - +0.1 5,253,138 - +1.5 5,253,153 - Pf1N1B4_4755 0.11 -1.5 5,253,156 - Pf1N1B4_4755 0.11 -0.5 5,253,453 + Pf1N1B4_4755 0.31 +1.3
Or see this region's nucleotide sequence