Experiment: Choline chloride 200 mM 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_4060 and Pf1N1B4_4061 are separated by 68 nucleotides Pf1N1B4_4061 and Pf1N1B4_4062 are separated by 172 nucleotides Pf1N1B4_4062 and Pf1N1B4_4063 overlap by 1 nucleotides Pf1N1B4_4063 and Pf1N1B4_4064 are separated by 15 nucleotides 
        Pf1N1B4_4060 - Cell division protein FtsK, at 4,513,027 to 4,515,432 
        _4060 
         
        
        Pf1N1B4_4061 - Outer membrane lipoprotein carrier protein LolA, at 4,515,501 to 4,516,124 
        _4061 
         
        
        Pf1N1B4_4062 - FIG065221: Holliday junction DNA helicase, at 4,516,297 to 4,517,619 
        _4062 
         
        
        Pf1N1B4_4063 - CrcB protein, at 4,517,619 to 4,517,993 
        _4063 
         
        
        Pf1N1B4_4064 - Seryl-tRNA synthetase (EC 6.1.1.11), at 4,518,009 to 4,519,289 
        _4064 
         Position (kb) 4516 
4517 
4518 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 at 4515.488 kb on + strand at 4516.123 kb on - strand at 4516.136 kb on + strand at 4516.136 kb on + strand at 4516.136 kb on + strand at 4516.137 kb on - strand at 4516.137 kb on - strand at 4516.137 kb on - strand at 4516.175 kb on + strand at 4516.175 kb on + strand at 4516.175 kb on + strand at 4516.176 kb on - strand at 4516.288 kb on + strand at 4516.288 kb on + strand at 4516.288 kb on + strand at 4516.288 kb on + strand at 4516.288 kb on + strand at 4516.289 kb on - strand at 4516.289 kb on - strand at 4516.289 kb on - strand at 4516.293 kb on - strand at 4516.473 kb on + strand, within Pf1N1B4_4062 at 4516.601 kb on - strand, within Pf1N1B4_4062 at 4516.768 kb on + strand, within Pf1N1B4_4062 at 4516.769 kb on - strand, within Pf1N1B4_4062 at 4517.041 kb on + strand, within Pf1N1B4_4062 at 4517.042 kb on - strand, within Pf1N1B4_4062 at 4517.042 kb on - strand, within Pf1N1B4_4062 at 4517.065 kb on - strand, within Pf1N1B4_4062 at 4517.143 kb on + strand, within Pf1N1B4_4062 at 4517.143 kb on + strand, within Pf1N1B4_4062 at 4517.144 kb on - strand, within Pf1N1B4_4062 at 4517.287 kb on - strand, within Pf1N1B4_4062 at 4517.290 kb on + strand, within Pf1N1B4_4062 at 4517.291 kb on - strand, within Pf1N1B4_4062 at 4517.291 kb on - strand, within Pf1N1B4_4062 at 4517.323 kb on + strand, within Pf1N1B4_4062 at 4517.324 kb on - strand, within Pf1N1B4_4062 at 4517.324 kb on - strand, within Pf1N1B4_4062 at 4517.488 kb on + strand at 4517.489 kb on - strand at 4517.489 kb on - strand at 4517.489 kb on - strand at 4517.594 kb on + strand at 4517.595 kb on - strand at 4517.721 kb on + strand, within Pf1N1B4_4063 at 4517.721 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.773 kb on + strand, within Pf1N1B4_4063 at 4517.941 kb on + strand, within Pf1N1B4_4063  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Choline chloride 200 mM remove 4,515,488 +  -0.2 4,516,123 -  -0.3 4,516,136 +  +0.4 4,516,136 +  -1.9 4,516,136 +  -0.6 4,516,137 -  +0.3 4,516,137 -  -2.8 4,516,137 -  -3.2 4,516,175 +  +0.4 4,516,175 +  -0.2 4,516,175 +  +0.7 4,516,176 -  +0.7 4,516,288 +  +1.3 4,516,288 +  -1.6 4,516,288 +  +1.4 4,516,288 +  -1.1 4,516,288 +  -1.5 4,516,289 -  +0.2 4,516,289 -  +0.7 4,516,289 -  -0.3 4,516,293 -  -0.3 4,516,473 +  Pf1N1B4_4062 0.13  -2.5 4,516,601 -  Pf1N1B4_4062 0.23  -1.9 4,516,768 +  Pf1N1B4_4062 0.36  -0.9 4,516,769 -  Pf1N1B4_4062 0.36  -0.0 4,517,041 +  Pf1N1B4_4062 0.56  +0.7 4,517,042 -  Pf1N1B4_4062 0.56  -0.6 4,517,042 -  Pf1N1B4_4062 0.56  -0.1 4,517,065 -  Pf1N1B4_4062 0.58  -1.3 4,517,143 +  Pf1N1B4_4062 0.64  -2.9 4,517,143 +  Pf1N1B4_4062 0.64  -2.9 4,517,144 -  Pf1N1B4_4062 0.64  +0.7 4,517,287 -  Pf1N1B4_4062 0.75  -0.5 4,517,290 +  Pf1N1B4_4062 0.75  -0.9 4,517,291 -  Pf1N1B4_4062 0.75  -0.9 4,517,291 -  Pf1N1B4_4062 0.75  -1.3 4,517,323 +  Pf1N1B4_4062 0.78  +0.3 4,517,324 -  Pf1N1B4_4062 0.78  -2.1 4,517,324 -  Pf1N1B4_4062 0.78  +0.1 4,517,488 +  -2.0 4,517,489 -  -1.1 4,517,489 -  +0.7 4,517,489 -  +0.1 4,517,594 +  -1.4 4,517,595 -  -0.6 4,517,721 +  Pf1N1B4_4063 0.27  -0.9 4,517,721 +  Pf1N1B4_4063 0.27  -2.1 4,517,773 +  Pf1N1B4_4063 0.41  +0.7 4,517,773 +  Pf1N1B4_4063 0.41  +0.7 4,517,773 +  Pf1N1B4_4063 0.41  -0.6 4,517,773 +  Pf1N1B4_4063 0.41  -0.0 4,517,773 +  Pf1N1B4_4063 0.41  -0.5 4,517,773 +  Pf1N1B4_4063 0.41  -0.6 4,517,941 +  Pf1N1B4_4063 0.86  -0.3 
Or see this region's nucleotide sequence