Experiment: Choline chloride 200 mM 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
     
    
        
         
     
    
        
         
     
    
        
         
     
    
         
     
     
 
500 nt Pf1N1B4_4046 and Pf1N1B4_4047 are separated by 127 nucleotides Pf1N1B4_4047 and Pf1N1B4_4048 overlap by 4 nucleotides Pf1N1B4_4048 and Pf1N1B4_4049 are separated by 59 nucleotides Pf1N1B4_4049 and Pf1N1B4_4050 are separated by 213 nucleotides Pf1N1B4_4050 and Pf1N1B4_4051 are separated by 38 nucleotides  
        
        Pf1N1B4_4046 - Adenylosuccinate lyase (EC 4.3.2.2), at 4,498,338 to 4,499,708 
         
        _4046 
         
        
        Pf1N1B4_4047 - FIG002903: a protein of unknown function perhaps involved in purine metabolism, at 4,499,836 to 4,500,459 
         
        _4047 
         
        
        Pf1N1B4_4048 - tRNA-specific 2-thiouridylase MnmA, at 4,500,456 to 4,501,589 
         
        _4048 
         
        
        Pf1N1B4_4049 - Nudix-like NDP and NTP phosphohydrolase YmfB, at 4,501,649 to 4,502,095 
         
        _4049 
         
        
        Pf1N1B4_4050 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,309 to 4,502,422 
         
        _4050 
         
        
        Pf1N1B4_4051 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 4,502,461 to 4,504,533 
         
        _4051 
         Position (kb)  
4500 
 
4501 
 
4502 Strain fitness (log2 ratio)  
-2 
 
-1 
 
0 
 
1 
 
2 
 
3 at 4499.795 kb on - strand at 4499.795 kb on - strand at 4499.795 kb on - strand at 4499.811 kb on + strand at 4499.821 kb on + strand at 4499.868 kb on + strand at 4499.950 kb on + strand at 4499.954 kb on - strand, within Pf1N1B4_4047 at 4499.954 kb on - strand, within Pf1N1B4_4047 at 4499.954 kb on - strand, within Pf1N1B4_4047 at 4500.053 kb on + strand, within Pf1N1B4_4047 at 4500.053 kb on + strand, within Pf1N1B4_4047 at 4500.054 kb on - strand, within Pf1N1B4_4047 at 4500.054 kb on - strand, within Pf1N1B4_4047 at 4500.054 kb on - strand, within Pf1N1B4_4047 at 4500.299 kb on + strand, within Pf1N1B4_4047 at 4500.299 kb on + strand, within Pf1N1B4_4047 at 4500.299 kb on + strand, within Pf1N1B4_4047 at 4500.300 kb on - strand, within Pf1N1B4_4047 at 4500.300 kb on - strand, within Pf1N1B4_4047 at 4501.709 kb on - strand at 4501.709 kb on - strand, within Pf1N1B4_4049 at 4501.806 kb on + strand, within Pf1N1B4_4049 at 4501.854 kb on + strand, within Pf1N1B4_4049 at 4501.854 kb on + strand, within Pf1N1B4_4049 at 4501.855 kb on - strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.881 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4501.887 kb on + strand, within Pf1N1B4_4049 at 4502.239 kb on + strand at 4502.239 kb on + strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.240 kb on - strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.243 kb on + strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.244 kb on - strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.247 kb on + strand at 4502.248 kb on - strand at 4502.248 kb on - strand at 4502.302 kb on + strand at 4502.310 kb on + strand at 4502.551 kb on - strand  
 
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction   Choline chloride 200 mM             remove   4,499,795   -        +1.7   4,499,795   -        -0.6   4,499,795   -        +1.3   4,499,811   +        +0.7   4,499,821   +        +0.7   4,499,868   +        +2.4   4,499,950   +        -0.3   4,499,954   -    Pf1N1B4_4047   0.19  +0.7   4,499,954   -    Pf1N1B4_4047   0.19  +1.0   4,499,954   -    Pf1N1B4_4047   0.19  -1.6   4,500,053   +    Pf1N1B4_4047   0.35  +0.7   4,500,053   +    Pf1N1B4_4047   0.35  +0.9   4,500,054   -    Pf1N1B4_4047   0.35  +0.2   4,500,054   -    Pf1N1B4_4047   0.35  +0.2   4,500,054   -    Pf1N1B4_4047   0.35  +0.4   4,500,299   +    Pf1N1B4_4047   0.74  -1.9   4,500,299   +    Pf1N1B4_4047   0.74  +1.3   4,500,299   +    Pf1N1B4_4047   0.74  +0.5   4,500,300   -    Pf1N1B4_4047   0.74  -0.7   4,500,300   -    Pf1N1B4_4047   0.74  +0.2   4,501,709   -        +2.7   4,501,709   -    Pf1N1B4_4049   0.13  -0.1   4,501,806   +    Pf1N1B4_4049   0.35  -1.3   4,501,854   +    Pf1N1B4_4049   0.46  -0.2   4,501,854   +    Pf1N1B4_4049   0.46  +0.9   4,501,855   -    Pf1N1B4_4049   0.46  +1.4   4,501,881   +    Pf1N1B4_4049   0.52  +0.3   4,501,881   +    Pf1N1B4_4049   0.52  +1.3   4,501,881   +    Pf1N1B4_4049   0.52  +1.4   4,501,887   +        -0.9   4,501,887   +    Pf1N1B4_4049   0.53  +1.1   4,501,887   +    Pf1N1B4_4049   0.53  +0.3   4,501,887   +    Pf1N1B4_4049   0.53  -1.1   4,501,887   +    Pf1N1B4_4049   0.53  +0.2   4,502,239   +        +0.4   4,502,239   +        -1.0   4,502,240   -        +0.1   4,502,240   -        -1.5   4,502,240   -        -1.1   4,502,240   -        -0.5   4,502,240   -        -0.0   4,502,240   -        +2.6   4,502,240   -        +1.7   4,502,240   -        -1.2   4,502,240   -        -0.6   4,502,240   -        +2.3   4,502,240   -        +1.4   4,502,243   +        +0.2   4,502,243   +        -0.9   4,502,243   +        -1.6   4,502,243   +        -0.9   4,502,243   +        +0.7   4,502,243   +        +1.3   4,502,243   +        +0.9   4,502,243   +        -0.9   4,502,243   +        -0.1   4,502,243   +        +0.1   4,502,243   +        +1.3   4,502,243   +        -0.3   4,502,243   +        +1.3   4,502,243   +        +0.4   4,502,244   -        -0.5   4,502,244   -        +0.9   4,502,244   -        -1.9   4,502,244   -        +3.5   4,502,244   -        -1.8   4,502,247   +        +0.0   4,502,247   +        -1.3   4,502,247   +        +0.2   4,502,247   +        -2.5   4,502,248   -        -0.9   4,502,248   -        +0.2   4,502,302   +        +0.7   4,502,310   +        -1.2   4,502,551   -        -0.3 
 
Or see this region's nucleotide sequence