Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3686 and Pf1N1B4_3687 are separated by 237 nucleotides Pf1N1B4_3687 and Pf1N1B4_3688 overlap by 1 nucleotides Pf1N1B4_3688 and Pf1N1B4_3689 are separated by 77 nucleotides Pf1N1B4_3689 and Pf1N1B4_3690 are separated by 157 nucleotides Pf1N1B4_3690 and Pf1N1B4_3691 overlap by 4 nucleotides
Pf1N1B4_3686 - Branched-chain amino acid transport system carrier protein, at 4,096,525 to 4,097,838
_3686
Pf1N1B4_3687 - FIG00953885: hypothetical protein, at 4,098,076 to 4,098,813
_3687
Pf1N1B4_3688 - FIG00956657: hypothetical protein, at 4,098,813 to 4,099,214
_3688
Pf1N1B4_3689 - Lipoprotein, putative, at 4,099,292 to 4,099,537
_3689
Pf1N1B4_3690 - FIG00956080: hypothetical protein, at 4,099,695 to 4,100,171
_3690
Pf1N1B4_3691 - FIG00953296: hypothetical protein, at 4,100,168 to 4,100,620
_3691
Position (kb)
4098
4099
4100 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4097.836 kb on + strand at 4097.836 kb on + strand at 4097.946 kb on - strand at 4097.946 kb on - strand at 4098.056 kb on + strand at 4098.056 kb on + strand at 4098.056 kb on + strand at 4098.057 kb on - strand at 4098.058 kb on + strand at 4098.058 kb on + strand at 4098.059 kb on - strand at 4098.469 kb on - strand at 4098.469 kb on - strand, within Pf1N1B4_3687 at 4098.469 kb on - strand, within Pf1N1B4_3687 at 4098.565 kb on - strand, within Pf1N1B4_3687 at 4098.637 kb on + strand, within Pf1N1B4_3687 at 4098.637 kb on + strand, within Pf1N1B4_3687 at 4098.637 kb on + strand, within Pf1N1B4_3687 at 4098.638 kb on - strand, within Pf1N1B4_3687 at 4098.638 kb on - strand at 4098.640 kb on + strand, within Pf1N1B4_3687 at 4098.721 kb on + strand, within Pf1N1B4_3687 at 4098.721 kb on + strand, within Pf1N1B4_3687 at 4098.763 kb on + strand at 4098.763 kb on + strand at 4098.763 kb on + strand at 4098.763 kb on + strand at 4098.763 kb on + strand at 4098.763 kb on + strand at 4098.765 kb on + strand at 4099.096 kb on + strand at 4099.096 kb on + strand, within Pf1N1B4_3688 at 4099.096 kb on + strand, within Pf1N1B4_3688 at 4099.096 kb on + strand, within Pf1N1B4_3688 at 4099.096 kb on + strand, within Pf1N1B4_3688 at 4099.096 kb on + strand, within Pf1N1B4_3688 at 4099.096 kb on + strand, within Pf1N1B4_3688 at 4099.096 kb on + strand, within Pf1N1B4_3688 at 4099.096 kb on + strand, within Pf1N1B4_3688 at 4099.096 kb on + strand, within Pf1N1B4_3688 at 4099.096 kb on + strand, within Pf1N1B4_3688 at 4099.096 kb on + strand, within Pf1N1B4_3688 at 4099.096 kb on + strand, within Pf1N1B4_3688 at 4099.096 kb on + strand, within Pf1N1B4_3688 at 4099.096 kb on + strand, within Pf1N1B4_3688 at 4099.097 kb on - strand, within Pf1N1B4_3688 at 4099.097 kb on - strand, within Pf1N1B4_3688 at 4099.097 kb on - strand, within Pf1N1B4_3688 at 4099.097 kb on - strand, within Pf1N1B4_3688 at 4099.097 kb on - strand, within Pf1N1B4_3688 at 4099.098 kb on + strand, within Pf1N1B4_3688 at 4099.098 kb on + strand, within Pf1N1B4_3688 at 4099.098 kb on + strand, within Pf1N1B4_3688 at 4099.098 kb on + strand, within Pf1N1B4_3688 at 4099.098 kb on + strand, within Pf1N1B4_3688 at 4099.110 kb on + strand, within Pf1N1B4_3688 at 4099.159 kb on + strand, within Pf1N1B4_3688 at 4099.339 kb on + strand, within Pf1N1B4_3689 at 4099.519 kb on + strand at 4099.520 kb on - strand at 4099.523 kb on + strand at 4099.524 kb on - strand at 4099.524 kb on - strand at 4099.524 kb on - strand at 4099.524 kb on - strand at 4099.562 kb on - strand at 4099.566 kb on - strand at 4099.595 kb on + strand at 4099.604 kb on + strand at 4099.605 kb on - strand at 4099.617 kb on + strand at 4099.618 kb on - strand at 4099.618 kb on - strand at 4099.618 kb on - strand at 4099.618 kb on - strand at 4099.618 kb on - strand at 4099.619 kb on + strand at 4099.620 kb on - strand at 4099.620 kb on - strand at 4099.620 kb on - strand at 4099.620 kb on - strand at 4099.622 kb on - strand at 4099.622 kb on - strand at 4099.622 kb on - strand at 4099.622 kb on - strand at 4099.622 kb on - strand at 4099.622 kb on - strand at 4099.622 kb on - strand at 4099.622 kb on - strand at 4099.622 kb on - strand at 4099.622 kb on - strand at 4099.622 kb on - strand at 4099.622 kb on - strand at 4099.622 kb on - strand at 4099.636 kb on - strand at 4099.678 kb on - strand at 4099.678 kb on - strand at 4099.678 kb on - strand at 4099.689 kb on - strand at 4099.760 kb on - strand, within Pf1N1B4_3690 at 4099.816 kb on - strand, within Pf1N1B4_3690 at 4099.962 kb on + strand, within Pf1N1B4_3690 at 4099.963 kb on - strand, within Pf1N1B4_3690 at 4099.963 kb on - strand at 4099.995 kb on + strand, within Pf1N1B4_3690 at 4099.995 kb on + strand, within Pf1N1B4_3690 at 4099.996 kb on - strand, within Pf1N1B4_3690 at 4100.014 kb on - strand, within Pf1N1B4_3690 at 4100.102 kb on + strand, within Pf1N1B4_3690 at 4100.153 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 4,097,836 + -0.2 4,097,836 + +0.4 4,097,946 - +0.4 4,097,946 - -1.0 4,098,056 + +1.4 4,098,056 + +0.9 4,098,056 + -1.0 4,098,057 - -1.2 4,098,058 + -1.2 4,098,058 + -0.2 4,098,059 - -1.5 4,098,469 - -0.2 4,098,469 - Pf1N1B4_3687 0.53 -1.5 4,098,469 - Pf1N1B4_3687 0.53 -0.0 4,098,565 - Pf1N1B4_3687 0.66 -1.1 4,098,637 + Pf1N1B4_3687 0.76 -0.2 4,098,637 + Pf1N1B4_3687 0.76 -2.0 4,098,637 + Pf1N1B4_3687 0.76 +0.2 4,098,638 - Pf1N1B4_3687 0.76 -2.0 4,098,638 - -0.2 4,098,640 + Pf1N1B4_3687 0.76 +0.8 4,098,721 + Pf1N1B4_3687 0.87 +0.2 4,098,721 + Pf1N1B4_3687 0.87 -1.5 4,098,763 + -1.2 4,098,763 + -0.5 4,098,763 + -1.1 4,098,763 + -0.8 4,098,763 + -1.6 4,098,763 + +2.1 4,098,765 + -0.9 4,099,096 + +0.8 4,099,096 + Pf1N1B4_3688 0.70 -1.2 4,099,096 + Pf1N1B4_3688 0.70 -1.8 4,099,096 + Pf1N1B4_3688 0.70 -1.2 4,099,096 + Pf1N1B4_3688 0.70 -0.2 4,099,096 + Pf1N1B4_3688 0.70 -0.2 4,099,096 + Pf1N1B4_3688 0.70 -1.0 4,099,096 + Pf1N1B4_3688 0.70 -2.0 4,099,096 + Pf1N1B4_3688 0.70 -0.8 4,099,096 + Pf1N1B4_3688 0.70 -0.8 4,099,096 + Pf1N1B4_3688 0.70 -0.2 4,099,096 + Pf1N1B4_3688 0.70 +0.8 4,099,096 + Pf1N1B4_3688 0.70 -0.8 4,099,096 + Pf1N1B4_3688 0.70 -0.2 4,099,096 + Pf1N1B4_3688 0.70 +0.2 4,099,097 - Pf1N1B4_3688 0.71 -1.0 4,099,097 - Pf1N1B4_3688 0.71 -1.5 4,099,097 - Pf1N1B4_3688 0.71 -2.0 4,099,097 - Pf1N1B4_3688 0.71 -0.2 4,099,097 - Pf1N1B4_3688 0.71 -1.4 4,099,098 + Pf1N1B4_3688 0.71 -1.8 4,099,098 + Pf1N1B4_3688 0.71 -1.2 4,099,098 + Pf1N1B4_3688 0.71 -0.8 4,099,098 + Pf1N1B4_3688 0.71 -1.2 4,099,098 + Pf1N1B4_3688 0.71 -2.6 4,099,110 + Pf1N1B4_3688 0.74 -2.2 4,099,159 + Pf1N1B4_3688 0.86 +1.6 4,099,339 + Pf1N1B4_3689 0.19 -0.2 4,099,519 + -0.2 4,099,520 - +0.2 4,099,523 + -0.2 4,099,524 - -1.9 4,099,524 - -1.1 4,099,524 - -1.8 4,099,524 - +0.1 4,099,562 - +0.8 4,099,566 - -0.9 4,099,595 + -0.4 4,099,604 + -1.5 4,099,605 - +0.1 4,099,617 + -0.2 4,099,618 - -2.4 4,099,618 - -0.2 4,099,618 - +1.1 4,099,618 - +0.2 4,099,618 - -0.2 4,099,619 + +0.2 4,099,620 - +0.1 4,099,620 - -0.0 4,099,620 - +0.5 4,099,620 - -2.5 4,099,622 - +0.3 4,099,622 - -0.2 4,099,622 - +1.1 4,099,622 - +1.0 4,099,622 - +0.7 4,099,622 - +0.7 4,099,622 - +1.2 4,099,622 - +1.7 4,099,622 - +0.0 4,099,622 - +0.3 4,099,622 - -0.5 4,099,622 - +1.3 4,099,622 - -0.6 4,099,636 - +0.4 4,099,678 - +0.2 4,099,678 - +0.4 4,099,678 - -0.2 4,099,689 - -1.0 4,099,760 - Pf1N1B4_3690 0.14 +1.1 4,099,816 - Pf1N1B4_3690 0.25 -1.2 4,099,962 + Pf1N1B4_3690 0.56 +0.5 4,099,963 - Pf1N1B4_3690 0.56 +0.1 4,099,963 - -0.8 4,099,995 + Pf1N1B4_3690 0.63 -0.3 4,099,995 + Pf1N1B4_3690 0.63 -1.7 4,099,996 - Pf1N1B4_3690 0.63 +0.2 4,100,014 - Pf1N1B4_3690 0.67 -0.2 4,100,102 + Pf1N1B4_3690 0.85 -2.4 4,100,153 - -0.8
Or see this region's nucleotide sequence