Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_355 and Pf1N1B4_356 are separated by 71 nucleotides Pf1N1B4_356 and Pf1N1B4_357 are separated by 4 nucleotides Pf1N1B4_357 and Pf1N1B4_358 are separated by 186 nucleotides
Pf1N1B4_355 - S-adenosylhomocysteine deaminase (EC 3.5.4.28); Methylthioadenosine deaminase, at 421,953 to 423,287
_355
Pf1N1B4_356 - 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64), at 423,359 to 424,057
_356
Pf1N1B4_357 - Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase, at 424,062 to 424,733
_357
Pf1N1B4_358 - Oxidoreductase, short chain dehydrogenase/reductase family, at 424,920 to 425,660
_358
Position (kb) 424
425 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 423.114 kb on + strand, within Pf1N1B4_355 at 423.115 kb on - strand, within Pf1N1B4_355 at 423.138 kb on + strand, within Pf1N1B4_355 at 423.138 kb on + strand, within Pf1N1B4_355 at 423.138 kb on + strand, within Pf1N1B4_355 at 423.138 kb on + strand at 423.138 kb on + strand, within Pf1N1B4_355 at 423.138 kb on + strand, within Pf1N1B4_355 at 423.138 kb on + strand, within Pf1N1B4_355 at 423.138 kb on + strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.139 kb on - strand, within Pf1N1B4_355 at 423.285 kb on + strand at 423.285 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.299 kb on + strand at 423.344 kb on + strand at 424.630 kb on - strand, within Pf1N1B4_357 at 424.793 kb on + strand at 424.793 kb on + strand at 424.793 kb on + strand at 424.794 kb on - strand at 424.794 kb on - strand at 424.794 kb on - strand at 424.794 kb on - strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.797 kb on + strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.798 kb on - strand at 424.838 kb on - strand at 424.883 kb on + strand at 424.883 kb on + strand at 424.883 kb on + strand at 424.883 kb on + strand at 424.883 kb on + strand at 424.919 kb on + strand at 424.919 kb on + strand at 424.920 kb on - strand at 424.920 kb on - strand at 424.920 kb on - strand at 424.930 kb on - strand at 424.930 kb on - strand at 425.017 kb on - strand, within Pf1N1B4_358 at 425.017 kb on - strand at 425.079 kb on + strand, within Pf1N1B4_358 at 425.079 kb on + strand, within Pf1N1B4_358 at 425.079 kb on + strand, within Pf1N1B4_358 at 425.079 kb on + strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.080 kb on - strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand, within Pf1N1B4_358 at 425.157 kb on + strand at 425.157 kb on + strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.158 kb on - strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.470 kb on + strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.471 kb on - strand, within Pf1N1B4_358 at 425.544 kb on + strand, within Pf1N1B4_358 at 425.598 kb on + strand at 425.598 kb on + strand at 425.598 kb on + strand at 425.599 kb on - strand at 425.599 kb on - strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.604 kb on + strand at 425.605 kb on - strand at 425.605 kb on - strand at 425.658 kb on + strand at 425.721 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 423,114 + Pf1N1B4_355 0.87 +1.3 423,115 - Pf1N1B4_355 0.87 -2.8 423,138 + Pf1N1B4_355 0.89 -1.9 423,138 + Pf1N1B4_355 0.89 -0.7 423,138 + Pf1N1B4_355 0.89 +0.3 423,138 + -1.1 423,138 + Pf1N1B4_355 0.89 -1.9 423,138 + Pf1N1B4_355 0.89 -0.4 423,138 + Pf1N1B4_355 0.89 -0.1 423,138 + Pf1N1B4_355 0.89 -1.5 423,139 - Pf1N1B4_355 0.89 -0.1 423,139 - Pf1N1B4_355 0.89 -0.4 423,139 - Pf1N1B4_355 0.89 +0.9 423,139 - Pf1N1B4_355 0.89 +3.7 423,139 - Pf1N1B4_355 0.89 -0.3 423,139 - Pf1N1B4_355 0.89 +0.3 423,139 - Pf1N1B4_355 0.89 +0.5 423,139 - Pf1N1B4_355 0.89 +0.3 423,139 - Pf1N1B4_355 0.89 +0.3 423,139 - Pf1N1B4_355 0.89 -1.7 423,139 - Pf1N1B4_355 0.89 -1.9 423,285 + +0.0 423,285 + +1.6 423,299 + +1.2 423,299 + -1.5 423,299 + +2.0 423,299 + +0.9 423,299 + +0.5 423,299 + -0.7 423,299 + -0.7 423,299 + +0.5 423,299 + -0.5 423,344 + +0.6 424,630 - Pf1N1B4_357 0.85 -2.3 424,793 + -0.1 424,793 + -0.1 424,793 + +1.0 424,794 - +0.9 424,794 - -0.3 424,794 - -1.3 424,794 - +0.5 424,797 + -0.7 424,797 + -0.1 424,797 + +1.1 424,797 + +0.0 424,797 + +1.0 424,797 + -1.5 424,797 + +0.9 424,798 - -0.5 424,798 - -1.0 424,798 - -1.7 424,798 - +2.0 424,798 - -0.9 424,798 - -1.9 424,798 - +0.2 424,798 - +1.9 424,798 - -0.7 424,798 - -0.3 424,798 - +1.9 424,798 - +1.4 424,798 - -0.7 424,838 - -0.1 424,883 + -0.1 424,883 + -0.2 424,883 + +0.6 424,883 + -1.0 424,883 + +0.9 424,919 + -0.7 424,919 + -2.5 424,920 - +0.9 424,920 - +1.0 424,920 - -1.1 424,930 - +0.1 424,930 - +0.9 425,017 - Pf1N1B4_358 0.13 -0.7 425,017 - +1.3 425,079 + Pf1N1B4_358 0.21 +0.2 425,079 + Pf1N1B4_358 0.21 +0.2 425,079 + Pf1N1B4_358 0.21 +1.1 425,079 + Pf1N1B4_358 0.21 -0.1 425,080 - Pf1N1B4_358 0.22 +1.3 425,080 - Pf1N1B4_358 0.22 -0.7 425,080 - Pf1N1B4_358 0.22 +0.5 425,080 - Pf1N1B4_358 0.22 +0.8 425,157 + Pf1N1B4_358 0.32 +2.3 425,157 + Pf1N1B4_358 0.32 -0.3 425,157 + Pf1N1B4_358 0.32 -0.7 425,157 + Pf1N1B4_358 0.32 +0.6 425,157 + Pf1N1B4_358 0.32 -0.1 425,157 + -1.7 425,157 + Pf1N1B4_358 0.32 +2.2 425,158 - Pf1N1B4_358 0.32 +0.5 425,158 - Pf1N1B4_358 0.32 +1.2 425,158 - Pf1N1B4_358 0.32 +0.9 425,158 - Pf1N1B4_358 0.32 -0.5 425,158 - Pf1N1B4_358 0.32 -1.7 425,470 + Pf1N1B4_358 0.74 +3.2 425,470 + Pf1N1B4_358 0.74 -0.9 425,470 + Pf1N1B4_358 0.74 -1.4 425,470 + Pf1N1B4_358 0.74 +0.3 425,470 + Pf1N1B4_358 0.74 +0.2 425,470 + Pf1N1B4_358 0.74 +0.1 425,471 - Pf1N1B4_358 0.74 +0.5 425,471 - Pf1N1B4_358 0.74 +1.3 425,471 - Pf1N1B4_358 0.74 -2.1 425,471 - Pf1N1B4_358 0.74 -1.1 425,471 - Pf1N1B4_358 0.74 -0.1 425,471 - Pf1N1B4_358 0.74 +1.3 425,471 - Pf1N1B4_358 0.74 -0.9 425,544 + Pf1N1B4_358 0.84 +0.5 425,598 + -0.1 425,598 + +0.7 425,598 + +0.1 425,599 - -0.7 425,599 - +0.5 425,604 + -0.9 425,604 + +0.3 425,604 + -0.4 425,604 + +1.6 425,604 + -2.1 425,604 + -1.3 425,605 - -0.7 425,605 - -0.4 425,658 + -0.0 425,721 - -0.3
Or see this region's nucleotide sequence