Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3323 and Pf1N1B4_3324 are separated by 30 nucleotides Pf1N1B4_3324 and Pf1N1B4_3325 are separated by 8 nucleotides Pf1N1B4_3325 and Pf1N1B4_3326 are separated by 243 nucleotides
Pf1N1B4_3323 - RNA polymerase sigma-H factor AlgT, at 3,711,515 to 3,712,096
_3323
Pf1N1B4_3324 - Sigma factor RpoE negative regulatory protein RseA, at 3,712,127 to 3,712,714
_3324
Pf1N1B4_3325 - Sigma factor RpoE negative regulatory protein RseB precursor, at 3,712,723 to 3,713,685
_3325
Pf1N1B4_3326 - HtrA protease/chaperone protein, at 3,713,929 to 3,715,359
_3326
Position (kb)
3712
3713
3714 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3712.743 kb on + strand at 3712.931 kb on + strand, within Pf1N1B4_3325 at 3713.412 kb on + strand, within Pf1N1B4_3325 at 3713.412 kb on + strand, within Pf1N1B4_3325 at 3713.574 kb on + strand, within Pf1N1B4_3325 at 3713.649 kb on + strand at 3713.649 kb on + strand at 3713.649 kb on + strand at 3713.649 kb on + strand at 3713.749 kb on + strand at 3713.806 kb on + strand at 3713.808 kb on + strand at 3713.901 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 3,712,743 + -3.5 3,712,931 + Pf1N1B4_3325 0.22 +0.1 3,713,412 + Pf1N1B4_3325 0.72 -0.6 3,713,412 + Pf1N1B4_3325 0.72 -1.8 3,713,574 + Pf1N1B4_3325 0.88 -1.0 3,713,649 + +1.0 3,713,649 + -0.0 3,713,649 + -1.8 3,713,649 + -0.0 3,713,749 + +0.9 3,713,806 + -0.6 3,713,808 + -0.7 3,713,901 + +0.1
Or see this region's nucleotide sequence