Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2990 and Pf1N1B4_2991 are separated by 42 nucleotides Pf1N1B4_2991 and Pf1N1B4_2992 are separated by 100 nucleotides Pf1N1B4_2992 and Pf1N1B4_2993 are separated by 154 nucleotides Pf1N1B4_2993 and Pf1N1B4_2994 are separated by 406 nucleotides
Pf1N1B4_2990 - NAD-dependent formate dehydrogenase gamma subunit, at 3,359,418 to 3,359,894
_2990
Pf1N1B4_2991 - hypothetical protein, at 3,359,937 to 3,360,206
_2991
Pf1N1B4_2992 - Potassium channel protein, at 3,360,307 to 3,360,648
_2992
Pf1N1B4_2993 - hypothetical protein, at 3,360,803 to 3,361,132
_2993
Pf1N1B4_2994 - ABC transporter, ATP-binding protein-related protein, at 3,361,539 to 3,363,155
_2994
Position (kb)
3360
3361
3362 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3359.868 kb on - strand at 3359.894 kb on + strand at 3359.894 kb on + strand at 3359.894 kb on + strand at 3359.895 kb on - strand at 3359.895 kb on - strand at 3359.895 kb on - strand at 3360.068 kb on + strand, within Pf1N1B4_2991 at 3360.145 kb on + strand, within Pf1N1B4_2991 at 3360.145 kb on + strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.151 kb on + strand, within Pf1N1B4_2991 at 3360.151 kb on + strand, within Pf1N1B4_2991 at 3360.151 kb on + strand, within Pf1N1B4_2991 at 3360.151 kb on + strand, within Pf1N1B4_2991 at 3360.152 kb on - strand, within Pf1N1B4_2991 at 3360.186 kb on + strand at 3360.186 kb on + strand at 3360.186 kb on + strand at 3360.186 kb on + strand at 3360.197 kb on + strand at 3360.198 kb on - strand at 3360.252 kb on - strand at 3360.287 kb on - strand at 3360.346 kb on + strand, within Pf1N1B4_2992 at 3360.347 kb on - strand, within Pf1N1B4_2992 at 3360.347 kb on - strand, within Pf1N1B4_2992 at 3360.347 kb on - strand at 3360.347 kb on - strand, within Pf1N1B4_2992 at 3360.347 kb on - strand, within Pf1N1B4_2992 at 3360.364 kb on + strand, within Pf1N1B4_2992 at 3360.364 kb on + strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.584 kb on + strand, within Pf1N1B4_2992 at 3360.585 kb on - strand, within Pf1N1B4_2992 at 3360.646 kb on + strand at 3360.646 kb on + strand at 3360.646 kb on + strand at 3360.646 kb on + strand at 3360.722 kb on + strand at 3360.722 kb on + strand at 3360.787 kb on - strand at 3360.829 kb on + strand at 3360.830 kb on - strand at 3360.830 kb on - strand at 3360.919 kb on + strand, within Pf1N1B4_2993 at 3360.920 kb on - strand, within Pf1N1B4_2993 at 3360.920 kb on - strand, within Pf1N1B4_2993 at 3360.923 kb on - strand, within Pf1N1B4_2993 at 3360.923 kb on - strand, within Pf1N1B4_2993 at 3360.923 kb on - strand, within Pf1N1B4_2993 at 3360.923 kb on - strand, within Pf1N1B4_2993 at 3360.923 kb on - strand, within Pf1N1B4_2993 at 3360.923 kb on - strand, within Pf1N1B4_2993 at 3360.970 kb on + strand, within Pf1N1B4_2993 at 3361.030 kb on - strand, within Pf1N1B4_2993 at 3361.030 kb on - strand, within Pf1N1B4_2993 at 3361.083 kb on + strand, within Pf1N1B4_2993 at 3361.083 kb on + strand, within Pf1N1B4_2993 at 3361.084 kb on - strand at 3361.084 kb on - strand, within Pf1N1B4_2993 at 3361.250 kb on - strand at 3361.370 kb on - strand at 3361.370 kb on - strand at 3361.375 kb on + strand at 3361.375 kb on + strand at 3361.375 kb on + strand at 3361.375 kb on + strand at 3361.376 kb on - strand at 3361.376 kb on - strand at 3361.376 kb on - strand at 3361.376 kb on - strand at 3361.376 kb on - strand at 3361.395 kb on + strand at 3361.395 kb on + strand at 3361.396 kb on - strand at 3361.396 kb on - strand at 3361.437 kb on - strand at 3361.452 kb on + strand at 3361.453 kb on - strand at 3361.453 kb on - strand at 3361.472 kb on + strand at 3361.472 kb on + strand at 3361.472 kb on + strand at 3361.472 kb on + strand at 3361.473 kb on - strand at 3361.473 kb on - strand at 3361.477 kb on - strand at 3361.477 kb on - strand at 3361.497 kb on + strand at 3361.497 kb on + strand at 3361.498 kb on - strand at 3361.503 kb on + strand at 3361.503 kb on + strand at 3361.503 kb on + strand at 3361.503 kb on + strand at 3361.503 kb on + strand at 3361.504 kb on - strand at 3361.504 kb on - strand at 3361.504 kb on - strand at 3361.504 kb on - strand at 3361.504 kb on - strand at 3361.522 kb on + strand at 3361.523 kb on - strand at 3361.523 kb on - strand at 3361.536 kb on - strand at 3361.536 kb on - strand at 3361.536 kb on - strand at 3361.587 kb on - strand at 3361.643 kb on + strand at 3361.643 kb on + strand at 3361.643 kb on + strand at 3361.643 kb on + strand at 3361.644 kb on - strand at 3361.644 kb on - strand at 3361.656 kb on + strand at 3361.684 kb on + strand at 3361.684 kb on + strand at 3361.684 kb on + strand at 3361.685 kb on - strand at 3361.685 kb on - strand at 3361.685 kb on - strand at 3361.685 kb on - strand at 3361.730 kb on - strand at 3361.739 kb on - strand at 3361.810 kb on + strand, within Pf1N1B4_2994 at 3361.846 kb on + strand, within Pf1N1B4_2994 at 3361.847 kb on - strand, within Pf1N1B4_2994 at 3361.876 kb on + strand, within Pf1N1B4_2994 at 3361.877 kb on - strand, within Pf1N1B4_2994 at 3361.877 kb on - strand, within Pf1N1B4_2994 at 3361.879 kb on + strand, within Pf1N1B4_2994 at 3361.879 kb on + strand, within Pf1N1B4_2994 at 3361.879 kb on + strand, within Pf1N1B4_2994 at 3361.880 kb on - strand, within Pf1N1B4_2994 at 3361.880 kb on - strand, within Pf1N1B4_2994 at 3361.880 kb on - strand, within Pf1N1B4_2994 at 3361.949 kb on + strand, within Pf1N1B4_2994 at 3361.955 kb on + strand, within Pf1N1B4_2994 at 3361.956 kb on - strand, within Pf1N1B4_2994 at 3361.997 kb on - strand, within Pf1N1B4_2994 at 3362.014 kb on + strand, within Pf1N1B4_2994 at 3362.062 kb on + strand, within Pf1N1B4_2994 at 3362.062 kb on + strand, within Pf1N1B4_2994 at 3362.062 kb on + strand, within Pf1N1B4_2994 at 3362.062 kb on + strand, within Pf1N1B4_2994 at 3362.063 kb on - strand, within Pf1N1B4_2994 at 3362.064 kb on + strand, within Pf1N1B4_2994 at 3362.064 kb on + strand, within Pf1N1B4_2994 at 3362.064 kb on + strand, within Pf1N1B4_2994 at 3362.064 kb on + strand, within Pf1N1B4_2994 at 3362.065 kb on - strand, within Pf1N1B4_2994 at 3362.066 kb on + strand at 3362.066 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 3,359,868 - -0.4 3,359,894 + +0.4 3,359,894 + +0.1 3,359,894 + -1.4 3,359,895 - -1.0 3,359,895 - +1.1 3,359,895 - +0.6 3,360,068 + Pf1N1B4_2991 0.49 -0.4 3,360,145 + Pf1N1B4_2991 0.77 +0.6 3,360,145 + Pf1N1B4_2991 0.77 -0.2 3,360,146 - Pf1N1B4_2991 0.77 -0.1 3,360,146 - Pf1N1B4_2991 0.77 -2.4 3,360,146 - Pf1N1B4_2991 0.77 +0.1 3,360,146 - Pf1N1B4_2991 0.77 +0.1 3,360,146 - Pf1N1B4_2991 0.77 -0.4 3,360,151 + Pf1N1B4_2991 0.79 -0.9 3,360,151 + Pf1N1B4_2991 0.79 +1.1 3,360,151 + Pf1N1B4_2991 0.79 +0.1 3,360,151 + Pf1N1B4_2991 0.79 -0.7 3,360,152 - Pf1N1B4_2991 0.80 -1.3 3,360,186 + -0.3 3,360,186 + -0.3 3,360,186 + -0.4 3,360,186 + +1.1 3,360,197 + -0.0 3,360,198 - -0.0 3,360,252 - -1.4 3,360,287 - +0.7 3,360,346 + Pf1N1B4_2992 0.11 +0.1 3,360,347 - Pf1N1B4_2992 0.12 +0.5 3,360,347 - Pf1N1B4_2992 0.12 -0.4 3,360,347 - -0.4 3,360,347 - Pf1N1B4_2992 0.12 +0.6 3,360,347 - Pf1N1B4_2992 0.12 +0.0 3,360,364 + Pf1N1B4_2992 0.17 -0.4 3,360,364 + Pf1N1B4_2992 0.17 +1.7 3,360,365 - Pf1N1B4_2992 0.17 -0.7 3,360,365 - Pf1N1B4_2992 0.17 -0.4 3,360,365 - Pf1N1B4_2992 0.17 -1.9 3,360,365 - Pf1N1B4_2992 0.17 +0.3 3,360,365 - Pf1N1B4_2992 0.17 -1.7 3,360,469 + Pf1N1B4_2992 0.47 -1.2 3,360,469 + Pf1N1B4_2992 0.47 +0.1 3,360,469 + Pf1N1B4_2992 0.47 -0.2 3,360,469 + Pf1N1B4_2992 0.47 +0.9 3,360,469 + Pf1N1B4_2992 0.47 -0.3 3,360,469 + Pf1N1B4_2992 0.47 +0.9 3,360,469 + Pf1N1B4_2992 0.47 -1.2 3,360,469 + Pf1N1B4_2992 0.47 -0.4 3,360,470 - Pf1N1B4_2992 0.48 +0.1 3,360,470 - Pf1N1B4_2992 0.48 +0.9 3,360,470 - Pf1N1B4_2992 0.48 -0.5 3,360,470 - Pf1N1B4_2992 0.48 -1.2 3,360,470 - Pf1N1B4_2992 0.48 -0.4 3,360,470 - Pf1N1B4_2992 0.48 -0.1 3,360,470 - Pf1N1B4_2992 0.48 +0.6 3,360,470 - Pf1N1B4_2992 0.48 +1.0 3,360,584 + Pf1N1B4_2992 0.81 -0.1 3,360,585 - Pf1N1B4_2992 0.81 -0.2 3,360,646 + +1.1 3,360,646 + +0.1 3,360,646 + +0.7 3,360,646 + +0.7 3,360,722 + -0.3 3,360,722 + +0.1 3,360,787 - -0.1 3,360,829 + -0.0 3,360,830 - -0.3 3,360,830 - +0.4 3,360,919 + Pf1N1B4_2993 0.35 -1.4 3,360,920 - Pf1N1B4_2993 0.35 +0.6 3,360,920 - Pf1N1B4_2993 0.35 -0.4 3,360,923 - Pf1N1B4_2993 0.36 -0.3 3,360,923 - Pf1N1B4_2993 0.36 -0.4 3,360,923 - Pf1N1B4_2993 0.36 -1.6 3,360,923 - Pf1N1B4_2993 0.36 +0.1 3,360,923 - Pf1N1B4_2993 0.36 -1.0 3,360,923 - Pf1N1B4_2993 0.36 -0.5 3,360,970 + Pf1N1B4_2993 0.51 +0.1 3,361,030 - Pf1N1B4_2993 0.69 -0.9 3,361,030 - Pf1N1B4_2993 0.69 -2.4 3,361,083 + Pf1N1B4_2993 0.85 -0.2 3,361,083 + Pf1N1B4_2993 0.85 -0.7 3,361,084 - +0.6 3,361,084 - Pf1N1B4_2993 0.85 -0.9 3,361,250 - +0.7 3,361,370 - -0.9 3,361,370 - -0.5 3,361,375 + +0.1 3,361,375 + -0.5 3,361,375 + +0.5 3,361,375 + -0.3 3,361,376 - -1.2 3,361,376 - -0.1 3,361,376 - +1.1 3,361,376 - +0.1 3,361,376 - -0.2 3,361,395 + -1.4 3,361,395 + +0.1 3,361,396 - -2.4 3,361,396 - -0.4 3,361,437 - +0.6 3,361,452 + -0.6 3,361,453 - -0.2 3,361,453 - +0.1 3,361,472 + +0.1 3,361,472 + -0.0 3,361,472 + -1.7 3,361,472 + -0.3 3,361,473 - -0.4 3,361,473 - -0.4 3,361,477 - +0.1 3,361,477 - -1.2 3,361,497 + +1.1 3,361,497 + +0.1 3,361,498 - -1.2 3,361,503 + -0.3 3,361,503 + -0.3 3,361,503 + +1.1 3,361,503 + -0.3 3,361,503 + +0.7 3,361,504 - +0.9 3,361,504 - +0.7 3,361,504 - +0.5 3,361,504 - -1.9 3,361,504 - -2.0 3,361,522 + -1.0 3,361,523 - -0.3 3,361,523 - +0.6 3,361,536 - -0.3 3,361,536 - -1.2 3,361,536 - -1.0 3,361,587 - +0.1 3,361,643 + -0.6 3,361,643 + -0.1 3,361,643 + -1.2 3,361,643 + -1.4 3,361,644 - -1.2 3,361,644 - -1.4 3,361,656 + -0.7 3,361,684 + +0.7 3,361,684 + -0.9 3,361,684 + +1.7 3,361,685 - +2.3 3,361,685 - -0.3 3,361,685 - +0.5 3,361,685 - +0.3 3,361,730 - +0.1 3,361,739 - +0.4 3,361,810 + Pf1N1B4_2994 0.17 +1.9 3,361,846 + Pf1N1B4_2994 0.19 -0.9 3,361,847 - Pf1N1B4_2994 0.19 +0.4 3,361,876 + Pf1N1B4_2994 0.21 -1.9 3,361,877 - Pf1N1B4_2994 0.21 +1.6 3,361,877 - Pf1N1B4_2994 0.21 -0.9 3,361,879 + Pf1N1B4_2994 0.21 -0.4 3,361,879 + Pf1N1B4_2994 0.21 +1.3 3,361,879 + Pf1N1B4_2994 0.21 +1.1 3,361,880 - Pf1N1B4_2994 0.21 +0.3 3,361,880 - Pf1N1B4_2994 0.21 +0.3 3,361,880 - Pf1N1B4_2994 0.21 +0.1 3,361,949 + Pf1N1B4_2994 0.25 -2.4 3,361,955 + Pf1N1B4_2994 0.26 +0.6 3,361,956 - Pf1N1B4_2994 0.26 +0.1 3,361,997 - Pf1N1B4_2994 0.28 +0.3 3,362,014 + Pf1N1B4_2994 0.29 -0.3 3,362,062 + Pf1N1B4_2994 0.32 +1.1 3,362,062 + Pf1N1B4_2994 0.32 +2.7 3,362,062 + Pf1N1B4_2994 0.32 -0.6 3,362,062 + Pf1N1B4_2994 0.32 -1.2 3,362,063 - Pf1N1B4_2994 0.32 -0.7 3,362,064 + Pf1N1B4_2994 0.32 +0.7 3,362,064 + Pf1N1B4_2994 0.32 -1.7 3,362,064 + Pf1N1B4_2994 0.32 -1.4 3,362,064 + Pf1N1B4_2994 0.32 -0.4 3,362,065 - Pf1N1B4_2994 0.33 -0.9 3,362,066 + +0.1 3,362,066 + +1.1
Or see this region's nucleotide sequence