Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2685 and Pf1N1B4_2686 are separated by 53 nucleotides Pf1N1B4_2686 and Pf1N1B4_2687 are separated by 176 nucleotides
Pf1N1B4_2685 - hypothetical protein, at 3,022,143 to 3,022,721
_2685
Pf1N1B4_2686 - DedA protein, at 3,022,775 to 3,024,091
_2686
Pf1N1B4_2687 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 3,024,268 to 3,024,966
_2687
Position (kb)
3022
3023
3024
3025 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3021.825 kb on + strand at 3021.826 kb on - strand at 3021.826 kb on - strand at 3021.826 kb on - strand at 3021.826 kb on - strand at 3021.848 kb on - strand at 3021.850 kb on - strand at 3021.850 kb on - strand at 3021.898 kb on + strand at 3021.933 kb on - strand at 3021.954 kb on + strand at 3022.065 kb on + strand at 3022.065 kb on + strand at 3022.066 kb on - strand at 3022.066 kb on - strand at 3022.067 kb on + strand at 3022.067 kb on + strand at 3022.072 kb on + strand at 3022.185 kb on + strand at 3022.232 kb on + strand, within Pf1N1B4_2685 at 3022.233 kb on - strand, within Pf1N1B4_2685 at 3022.310 kb on + strand, within Pf1N1B4_2685 at 3022.310 kb on + strand, within Pf1N1B4_2685 at 3022.311 kb on - strand, within Pf1N1B4_2685 at 3022.380 kb on + strand, within Pf1N1B4_2685 at 3022.381 kb on - strand, within Pf1N1B4_2685 at 3022.455 kb on + strand, within Pf1N1B4_2685 at 3022.455 kb on + strand, within Pf1N1B4_2685 at 3022.455 kb on + strand, within Pf1N1B4_2685 at 3022.456 kb on - strand, within Pf1N1B4_2685 at 3022.457 kb on + strand, within Pf1N1B4_2685 at 3022.458 kb on - strand, within Pf1N1B4_2685 at 3022.496 kb on + strand, within Pf1N1B4_2685 at 3022.496 kb on + strand, within Pf1N1B4_2685 at 3022.496 kb on + strand, within Pf1N1B4_2685 at 3022.497 kb on - strand, within Pf1N1B4_2685 at 3022.497 kb on - strand, within Pf1N1B4_2685 at 3022.497 kb on - strand, within Pf1N1B4_2685 at 3022.698 kb on - strand at 3022.756 kb on + strand at 3022.756 kb on + strand at 3022.776 kb on + strand at 3022.777 kb on - strand at 3022.779 kb on + strand at 3022.779 kb on + strand at 3022.779 kb on + strand at 3022.780 kb on - strand at 3022.780 kb on - strand at 3022.780 kb on - strand at 3022.785 kb on + strand at 3022.958 kb on + strand at 3022.959 kb on - strand, within Pf1N1B4_2686 at 3022.959 kb on - strand, within Pf1N1B4_2686 at 3022.960 kb on + strand, within Pf1N1B4_2686 at 3022.960 kb on + strand, within Pf1N1B4_2686 at 3022.960 kb on + strand, within Pf1N1B4_2686 at 3022.961 kb on - strand, within Pf1N1B4_2686 at 3022.961 kb on - strand, within Pf1N1B4_2686 at 3022.986 kb on + strand, within Pf1N1B4_2686 at 3022.986 kb on + strand at 3022.987 kb on - strand, within Pf1N1B4_2686 at 3023.137 kb on - strand, within Pf1N1B4_2686 at 3023.137 kb on - strand, within Pf1N1B4_2686 at 3023.137 kb on - strand, within Pf1N1B4_2686 at 3023.137 kb on - strand, within Pf1N1B4_2686 at 3023.516 kb on + strand, within Pf1N1B4_2686 at 3023.516 kb on + strand, within Pf1N1B4_2686 at 3023.619 kb on + strand, within Pf1N1B4_2686 at 3023.619 kb on + strand, within Pf1N1B4_2686 at 3023.706 kb on - strand, within Pf1N1B4_2686 at 3023.772 kb on + strand, within Pf1N1B4_2686 at 3023.772 kb on + strand, within Pf1N1B4_2686 at 3023.772 kb on + strand, within Pf1N1B4_2686 at 3023.772 kb on + strand, within Pf1N1B4_2686 at 3023.853 kb on - strand, within Pf1N1B4_2686 at 3023.860 kb on - strand, within Pf1N1B4_2686 at 3023.900 kb on + strand, within Pf1N1B4_2686 at 3023.963 kb on + strand at 3023.963 kb on + strand at 3023.963 kb on + strand at 3023.964 kb on - strand at 3023.964 kb on - strand at 3023.964 kb on - strand at 3023.964 kb on - strand at 3023.964 kb on - strand at 3023.964 kb on - strand at 3024.074 kb on - strand at 3024.207 kb on + strand at 3024.207 kb on + strand at 3024.208 kb on - strand at 3024.208 kb on - strand at 3024.208 kb on - strand at 3024.208 kb on - strand at 3024.332 kb on + strand at 3024.362 kb on + strand, within Pf1N1B4_2687 at 3024.363 kb on - strand, within Pf1N1B4_2687 at 3024.650 kb on - strand, within Pf1N1B4_2687 at 3024.704 kb on + strand, within Pf1N1B4_2687 at 3024.707 kb on + strand, within Pf1N1B4_2687 at 3024.707 kb on + strand, within Pf1N1B4_2687 at 3024.707 kb on + strand, within Pf1N1B4_2687 at 3024.707 kb on + strand, within Pf1N1B4_2687 at 3024.707 kb on + strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.711 kb on + strand, within Pf1N1B4_2687 at 3024.711 kb on + strand, within Pf1N1B4_2687 at 3024.711 kb on + strand, within Pf1N1B4_2687 at 3024.711 kb on + strand at 3024.712 kb on - strand, within Pf1N1B4_2687 at 3024.712 kb on - strand, within Pf1N1B4_2687 at 3024.712 kb on - strand, within Pf1N1B4_2687 at 3024.712 kb on - strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.744 kb on + strand, within Pf1N1B4_2687 at 3024.794 kb on + strand, within Pf1N1B4_2687 at 3024.862 kb on - strand, within Pf1N1B4_2687 at 3025.012 kb on + strand at 3025.022 kb on - strand at 3025.022 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 3,021,825 + -1.1 3,021,826 - -0.5 3,021,826 - +0.1 3,021,826 - +0.2 3,021,826 - -0.3 3,021,848 - -0.1 3,021,850 - -0.7 3,021,850 - +1.0 3,021,898 + +1.2 3,021,933 - -0.7 3,021,954 + -0.8 3,022,065 + -1.6 3,022,065 + -1.9 3,022,066 - -0.8 3,022,066 - +0.2 3,022,067 + -0.6 3,022,067 + -0.3 3,022,072 + +0.2 3,022,185 + -1.5 3,022,232 + Pf1N1B4_2685 0.15 -1.7 3,022,233 - Pf1N1B4_2685 0.16 -0.6 3,022,310 + Pf1N1B4_2685 0.29 -1.3 3,022,310 + Pf1N1B4_2685 0.29 +1.2 3,022,311 - Pf1N1B4_2685 0.29 +0.8 3,022,380 + Pf1N1B4_2685 0.41 +0.8 3,022,381 - Pf1N1B4_2685 0.41 +0.7 3,022,455 + Pf1N1B4_2685 0.54 +0.6 3,022,455 + Pf1N1B4_2685 0.54 +0.7 3,022,455 + Pf1N1B4_2685 0.54 -0.3 3,022,456 - Pf1N1B4_2685 0.54 -0.2 3,022,457 + Pf1N1B4_2685 0.54 +0.9 3,022,458 - Pf1N1B4_2685 0.54 +0.4 3,022,496 + Pf1N1B4_2685 0.61 -1.8 3,022,496 + Pf1N1B4_2685 0.61 +1.2 3,022,496 + Pf1N1B4_2685 0.61 -0.1 3,022,497 - Pf1N1B4_2685 0.61 -0.1 3,022,497 - Pf1N1B4_2685 0.61 +1.2 3,022,497 - Pf1N1B4_2685 0.61 +0.4 3,022,698 - +1.2 3,022,756 + -0.8 3,022,756 + -1.3 3,022,776 + -0.1 3,022,777 - -0.2 3,022,779 + -0.1 3,022,779 + +0.2 3,022,779 + -0.8 3,022,780 - -1.0 3,022,780 - +1.8 3,022,780 - +0.3 3,022,785 + -2.3 3,022,958 + +0.2 3,022,959 - Pf1N1B4_2686 0.14 -2.3 3,022,959 - Pf1N1B4_2686 0.14 -0.5 3,022,960 + Pf1N1B4_2686 0.14 -1.5 3,022,960 + Pf1N1B4_2686 0.14 +0.6 3,022,960 + Pf1N1B4_2686 0.14 +0.4 3,022,961 - Pf1N1B4_2686 0.14 +1.5 3,022,961 - Pf1N1B4_2686 0.14 -1.6 3,022,986 + Pf1N1B4_2686 0.16 -0.0 3,022,986 + +0.2 3,022,987 - Pf1N1B4_2686 0.16 -2.2 3,023,137 - Pf1N1B4_2686 0.27 -1.6 3,023,137 - Pf1N1B4_2686 0.27 +2.2 3,023,137 - Pf1N1B4_2686 0.27 -1.3 3,023,137 - Pf1N1B4_2686 0.27 -1.6 3,023,516 + Pf1N1B4_2686 0.56 -0.8 3,023,516 + Pf1N1B4_2686 0.56 -1.2 3,023,619 + Pf1N1B4_2686 0.64 -0.9 3,023,619 + Pf1N1B4_2686 0.64 -1.8 3,023,706 - Pf1N1B4_2686 0.71 -1.1 3,023,772 + Pf1N1B4_2686 0.76 -0.1 3,023,772 + Pf1N1B4_2686 0.76 -0.6 3,023,772 + Pf1N1B4_2686 0.76 -0.8 3,023,772 + Pf1N1B4_2686 0.76 -1.3 3,023,853 - Pf1N1B4_2686 0.82 -0.3 3,023,860 - Pf1N1B4_2686 0.82 -1.6 3,023,900 + Pf1N1B4_2686 0.85 +0.2 3,023,963 + +3.2 3,023,963 + -0.6 3,023,963 + +0.7 3,023,964 - -0.1 3,023,964 - -1.1 3,023,964 - +1.5 3,023,964 - -0.8 3,023,964 - -0.4 3,023,964 - -1.3 3,024,074 - +0.7 3,024,207 + -0.3 3,024,207 + +1.2 3,024,208 - -1.1 3,024,208 - -1.5 3,024,208 - +1.5 3,024,208 - -0.0 3,024,332 + -0.6 3,024,362 + Pf1N1B4_2687 0.13 +1.2 3,024,363 - Pf1N1B4_2687 0.14 -1.2 3,024,650 - Pf1N1B4_2687 0.55 +2.2 3,024,704 + Pf1N1B4_2687 0.62 -1.0 3,024,707 + Pf1N1B4_2687 0.63 -0.8 3,024,707 + Pf1N1B4_2687 0.63 -0.5 3,024,707 + Pf1N1B4_2687 0.63 -1.3 3,024,707 + Pf1N1B4_2687 0.63 +0.2 3,024,707 + Pf1N1B4_2687 0.63 -0.8 3,024,708 - Pf1N1B4_2687 0.63 -1.3 3,024,708 - Pf1N1B4_2687 0.63 +0.2 3,024,708 - Pf1N1B4_2687 0.63 +0.1 3,024,708 - Pf1N1B4_2687 0.63 +0.8 3,024,708 - Pf1N1B4_2687 0.63 -0.2 3,024,708 - Pf1N1B4_2687 0.63 -0.8 3,024,708 - Pf1N1B4_2687 0.63 -1.1 3,024,708 - Pf1N1B4_2687 0.63 -0.8 3,024,708 - Pf1N1B4_2687 0.63 +1.2 3,024,708 - Pf1N1B4_2687 0.63 -0.8 3,024,711 + Pf1N1B4_2687 0.63 -1.2 3,024,711 + Pf1N1B4_2687 0.63 +0.7 3,024,711 + Pf1N1B4_2687 0.63 -1.1 3,024,711 + -0.3 3,024,712 - Pf1N1B4_2687 0.64 +0.2 3,024,712 - Pf1N1B4_2687 0.64 -0.3 3,024,712 - Pf1N1B4_2687 0.64 -1.8 3,024,712 - Pf1N1B4_2687 0.64 -1.0 3,024,713 + Pf1N1B4_2687 0.64 -2.1 3,024,713 + Pf1N1B4_2687 0.64 +1.2 3,024,713 + Pf1N1B4_2687 0.64 +0.2 3,024,713 + Pf1N1B4_2687 0.64 +0.2 3,024,713 + Pf1N1B4_2687 0.64 -1.6 3,024,713 + Pf1N1B4_2687 0.64 +1.0 3,024,713 + Pf1N1B4_2687 0.64 -0.8 3,024,713 + Pf1N1B4_2687 0.64 -0.8 3,024,713 + Pf1N1B4_2687 0.64 +1.4 3,024,713 + Pf1N1B4_2687 0.64 -1.8 3,024,713 + Pf1N1B4_2687 0.64 -0.7 3,024,713 + Pf1N1B4_2687 0.64 -1.3 3,024,713 + Pf1N1B4_2687 0.64 +0.2 3,024,714 - Pf1N1B4_2687 0.64 +0.0 3,024,714 - -0.3 3,024,714 - Pf1N1B4_2687 0.64 -0.2 3,024,714 - +3.6 3,024,714 - Pf1N1B4_2687 0.64 -0.8 3,024,714 - Pf1N1B4_2687 0.64 +1.6 3,024,714 - Pf1N1B4_2687 0.64 -0.8 3,024,714 - Pf1N1B4_2687 0.64 +0.8 3,024,714 - Pf1N1B4_2687 0.64 +0.4 3,024,714 - Pf1N1B4_2687 0.64 +1.2 3,024,714 - Pf1N1B4_2687 0.64 -0.2 3,024,714 - Pf1N1B4_2687 0.64 -2.1 3,024,714 - Pf1N1B4_2687 0.64 +0.7 3,024,714 - Pf1N1B4_2687 0.64 -0.8 3,024,714 - Pf1N1B4_2687 0.64 -0.3 3,024,714 - Pf1N1B4_2687 0.64 -1.2 3,024,744 + Pf1N1B4_2687 0.68 -1.6 3,024,794 + Pf1N1B4_2687 0.75 +0.2 3,024,862 - Pf1N1B4_2687 0.85 +1.2 3,025,012 + +0.2 3,025,022 - +1.2 3,025,022 - -0.8
Or see this region's nucleotide sequence