Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2389 and Pf1N1B4_2390 are separated by 185 nucleotides Pf1N1B4_2390 and Pf1N1B4_2391 are separated by 116 nucleotides Pf1N1B4_2391 and Pf1N1B4_2392 are separated by 128 nucleotides Pf1N1B4_2392 and Pf1N1B4_2393 are separated by 42 nucleotides
Pf1N1B4_2389 - Integral membrane protein, at 2,707,335 to 2,708,168
_2389
Pf1N1B4_2390 - probable membrane protein NMA1176, at 2,708,354 to 2,708,602
_2390
Pf1N1B4_2391 - Membrane-bound lytic murein transglycosylase A precursor (EC 3.2.1.-), at 2,708,719 to 2,709,891
_2391
Pf1N1B4_2392 - Cytochrome c556, at 2,710,020 to 2,710,469
_2392
Pf1N1B4_2393 - Protein yqjC precursor, at 2,710,512 to 2,710,907
_2393
Position (kb)
2708
2709
2710 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2707.800 kb on + strand, within Pf1N1B4_2389 at 2707.800 kb on + strand, within Pf1N1B4_2389 at 2707.800 kb on + strand, within Pf1N1B4_2389 at 2707.801 kb on - strand, within Pf1N1B4_2389 at 2707.801 kb on - strand, within Pf1N1B4_2389 at 2707.851 kb on + strand, within Pf1N1B4_2389 at 2707.851 kb on + strand, within Pf1N1B4_2389 at 2707.978 kb on + strand, within Pf1N1B4_2389 at 2707.989 kb on + strand, within Pf1N1B4_2389 at 2707.989 kb on + strand, within Pf1N1B4_2389 at 2707.989 kb on + strand, within Pf1N1B4_2389 at 2707.989 kb on + strand, within Pf1N1B4_2389 at 2707.989 kb on + strand, within Pf1N1B4_2389 at 2707.989 kb on + strand, within Pf1N1B4_2389 at 2707.989 kb on + strand, within Pf1N1B4_2389 at 2707.990 kb on - strand, within Pf1N1B4_2389 at 2708.088 kb on + strand at 2708.089 kb on - strand at 2708.122 kb on + strand at 2708.166 kb on + strand at 2708.194 kb on + strand at 2708.196 kb on + strand at 2708.264 kb on + strand at 2708.264 kb on + strand at 2708.264 kb on + strand at 2708.264 kb on + strand at 2708.264 kb on + strand at 2708.264 kb on + strand at 2708.264 kb on + strand at 2708.265 kb on - strand at 2708.274 kb on + strand at 2708.274 kb on + strand at 2708.274 kb on + strand at 2708.274 kb on + strand at 2708.274 kb on + strand at 2708.275 kb on - strand at 2708.309 kb on - strand at 2708.516 kb on + strand, within Pf1N1B4_2390 at 2708.516 kb on + strand, within Pf1N1B4_2390 at 2708.516 kb on + strand, within Pf1N1B4_2390 at 2708.517 kb on - strand, within Pf1N1B4_2390 at 2708.550 kb on + strand, within Pf1N1B4_2390 at 2708.550 kb on + strand, within Pf1N1B4_2390 at 2708.550 kb on + strand, within Pf1N1B4_2390 at 2708.551 kb on - strand, within Pf1N1B4_2390 at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.639 kb on + strand at 2708.640 kb on - strand at 2708.720 kb on + strand at 2708.720 kb on + strand at 2708.721 kb on - strand at 2708.721 kb on - strand at 2708.721 kb on - strand at 2708.721 kb on - strand at 2708.735 kb on - strand at 2708.735 kb on - strand at 2708.735 kb on - strand at 2708.883 kb on + strand, within Pf1N1B4_2391 at 2708.989 kb on - strand, within Pf1N1B4_2391 at 2708.990 kb on + strand, within Pf1N1B4_2391 at 2708.990 kb on + strand, within Pf1N1B4_2391 at 2708.991 kb on - strand, within Pf1N1B4_2391 at 2709.065 kb on + strand, within Pf1N1B4_2391 at 2709.065 kb on + strand at 2709.066 kb on - strand at 2709.066 kb on - strand, within Pf1N1B4_2391 at 2709.066 kb on - strand, within Pf1N1B4_2391 at 2709.425 kb on + strand, within Pf1N1B4_2391 at 2709.425 kb on + strand, within Pf1N1B4_2391 at 2709.425 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.429 kb on + strand, within Pf1N1B4_2391 at 2709.430 kb on + strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.430 kb on - strand, within Pf1N1B4_2391 at 2709.470 kb on + strand, within Pf1N1B4_2391 at 2709.470 kb on + strand, within Pf1N1B4_2391 at 2709.470 kb on + strand, within Pf1N1B4_2391 at 2709.470 kb on + strand, within Pf1N1B4_2391 at 2709.471 kb on - strand, within Pf1N1B4_2391 at 2709.471 kb on - strand, within Pf1N1B4_2391 at 2709.471 kb on - strand, within Pf1N1B4_2391 at 2709.471 kb on - strand, within Pf1N1B4_2391 at 2709.527 kb on + strand, within Pf1N1B4_2391 at 2709.527 kb on + strand, within Pf1N1B4_2391 at 2709.527 kb on + strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.528 kb on - strand, within Pf1N1B4_2391 at 2709.578 kb on + strand, within Pf1N1B4_2391 at 2709.579 kb on - strand, within Pf1N1B4_2391 at 2709.579 kb on - strand, within Pf1N1B4_2391 at 2709.687 kb on + strand, within Pf1N1B4_2391 at 2709.770 kb on + strand, within Pf1N1B4_2391 at 2709.770 kb on + strand, within Pf1N1B4_2391 at 2709.771 kb on - strand, within Pf1N1B4_2391 at 2709.896 kb on + strand at 2709.896 kb on + strand at 2709.897 kb on - strand at 2709.897 kb on - strand at 2709.897 kb on - strand at 2709.950 kb on + strand at 2709.950 kb on + strand at 2709.953 kb on + strand at 2709.953 kb on + strand at 2709.953 kb on + strand at 2709.953 kb on + strand at 2709.954 kb on - strand at 2709.954 kb on - strand at 2710.028 kb on + strand at 2710.029 kb on - strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.046 kb on - strand at 2710.046 kb on - strand at 2710.046 kb on - strand at 2710.046 kb on - strand at 2710.175 kb on + strand, within Pf1N1B4_2392 at 2710.175 kb on + strand, within Pf1N1B4_2392 at 2710.175 kb on + strand, within Pf1N1B4_2392 at 2710.176 kb on - strand, within Pf1N1B4_2392 at 2710.386 kb on + strand, within Pf1N1B4_2392 at 2710.386 kb on + strand, within Pf1N1B4_2392 at 2710.387 kb on - strand, within Pf1N1B4_2392 at 2710.387 kb on - strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.514 kb on - strand at 2710.514 kb on - strand at 2710.514 kb on - strand at 2710.517 kb on + strand at 2710.517 kb on + strand at 2710.517 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 2,707,800 + Pf1N1B4_2389 0.56 +0.5 2,707,800 + Pf1N1B4_2389 0.56 +0.9 2,707,800 + Pf1N1B4_2389 0.56 +2.9 2,707,801 - Pf1N1B4_2389 0.56 +2.1 2,707,801 - Pf1N1B4_2389 0.56 +0.8 2,707,851 + Pf1N1B4_2389 0.62 -1.5 2,707,851 + Pf1N1B4_2389 0.62 -0.5 2,707,978 + Pf1N1B4_2389 0.77 -0.2 2,707,989 + Pf1N1B4_2389 0.78 +1.1 2,707,989 + Pf1N1B4_2389 0.78 +0.3 2,707,989 + Pf1N1B4_2389 0.78 -1.0 2,707,989 + Pf1N1B4_2389 0.78 -1.2 2,707,989 + Pf1N1B4_2389 0.78 -0.3 2,707,989 + Pf1N1B4_2389 0.78 -0.8 2,707,989 + Pf1N1B4_2389 0.78 -0.3 2,707,990 - Pf1N1B4_2389 0.79 +1.1 2,708,088 + +0.1 2,708,089 - +1.3 2,708,122 + +0.4 2,708,166 + +0.8 2,708,194 + -1.4 2,708,196 + +0.9 2,708,264 + -1.6 2,708,264 + +0.5 2,708,264 + -0.6 2,708,264 + +1.0 2,708,264 + -2.7 2,708,264 + +0.1 2,708,264 + -0.7 2,708,265 - -1.5 2,708,274 + -2.6 2,708,274 + +2.0 2,708,274 + +1.3 2,708,274 + -0.3 2,708,274 + +0.6 2,708,275 - +1.0 2,708,309 - -1.0 2,708,516 + Pf1N1B4_2390 0.65 +0.3 2,708,516 + Pf1N1B4_2390 0.65 -1.5 2,708,516 + Pf1N1B4_2390 0.65 +1.6 2,708,517 - Pf1N1B4_2390 0.65 +1.3 2,708,550 + Pf1N1B4_2390 0.79 +0.3 2,708,550 + Pf1N1B4_2390 0.79 -0.2 2,708,550 + Pf1N1B4_2390 0.79 -0.0 2,708,551 - Pf1N1B4_2390 0.79 +0.2 2,708,639 + +0.2 2,708,639 + -1.0 2,708,639 + -0.7 2,708,639 + -0.8 2,708,639 + +1.3 2,708,639 + -0.3 2,708,639 + -1.0 2,708,639 + -0.7 2,708,639 + +0.3 2,708,640 - -0.2 2,708,720 + +0.3 2,708,720 + +0.6 2,708,721 - +0.4 2,708,721 - +1.0 2,708,721 - -1.9 2,708,721 - -1.0 2,708,735 - +0.3 2,708,735 - -1.5 2,708,735 - +1.0 2,708,883 + Pf1N1B4_2391 0.14 -2.3 2,708,989 - Pf1N1B4_2391 0.23 +1.0 2,708,990 + Pf1N1B4_2391 0.23 +0.3 2,708,990 + Pf1N1B4_2391 0.23 +1.8 2,708,991 - Pf1N1B4_2391 0.23 -1.3 2,709,065 + Pf1N1B4_2391 0.29 +0.3 2,709,065 + -0.3 2,709,066 - -0.3 2,709,066 - Pf1N1B4_2391 0.30 -0.6 2,709,066 - Pf1N1B4_2391 0.30 +0.8 2,709,425 + Pf1N1B4_2391 0.60 +0.8 2,709,425 + Pf1N1B4_2391 0.60 +0.6 2,709,425 + Pf1N1B4_2391 0.60 +1.3 2,709,429 + Pf1N1B4_2391 0.61 -0.7 2,709,429 + Pf1N1B4_2391 0.61 -0.3 2,709,429 + Pf1N1B4_2391 0.61 -0.5 2,709,429 + Pf1N1B4_2391 0.61 -0.1 2,709,429 + Pf1N1B4_2391 0.61 +0.5 2,709,429 + Pf1N1B4_2391 0.61 +0.4 2,709,429 + Pf1N1B4_2391 0.61 +0.7 2,709,429 + Pf1N1B4_2391 0.61 +1.0 2,709,429 + Pf1N1B4_2391 0.61 -0.3 2,709,429 + Pf1N1B4_2391 0.61 +0.3 2,709,429 + +1.3 2,709,429 + Pf1N1B4_2391 0.61 +2.7 2,709,429 + Pf1N1B4_2391 0.61 +0.3 2,709,429 + Pf1N1B4_2391 0.61 +0.0 2,709,429 + Pf1N1B4_2391 0.61 -0.0 2,709,429 + Pf1N1B4_2391 0.61 +1.7 2,709,429 + Pf1N1B4_2391 0.61 +2.0 2,709,429 + Pf1N1B4_2391 0.61 +0.7 2,709,429 + Pf1N1B4_2391 0.61 -0.3 2,709,429 + Pf1N1B4_2391 0.61 +0.3 2,709,429 + Pf1N1B4_2391 0.61 +0.3 2,709,430 + Pf1N1B4_2391 0.61 -0.6 2,709,430 - Pf1N1B4_2391 0.61 +0.9 2,709,430 - Pf1N1B4_2391 0.61 +1.3 2,709,430 - Pf1N1B4_2391 0.61 -1.5 2,709,430 - Pf1N1B4_2391 0.61 +3.9 2,709,430 - Pf1N1B4_2391 0.61 -0.2 2,709,430 - Pf1N1B4_2391 0.61 +0.5 2,709,430 - Pf1N1B4_2391 0.61 +1.0 2,709,430 - Pf1N1B4_2391 0.61 +1.1 2,709,430 - Pf1N1B4_2391 0.61 -1.0 2,709,430 - Pf1N1B4_2391 0.61 +0.6 2,709,430 - Pf1N1B4_2391 0.61 -0.0 2,709,430 - Pf1N1B4_2391 0.61 -0.0 2,709,430 - Pf1N1B4_2391 0.61 +0.5 2,709,430 - Pf1N1B4_2391 0.61 +0.3 2,709,430 - Pf1N1B4_2391 0.61 -0.9 2,709,470 + Pf1N1B4_2391 0.64 -0.7 2,709,470 + Pf1N1B4_2391 0.64 +2.1 2,709,470 + Pf1N1B4_2391 0.64 +0.3 2,709,470 + Pf1N1B4_2391 0.64 -0.9 2,709,471 - Pf1N1B4_2391 0.64 +0.4 2,709,471 - Pf1N1B4_2391 0.64 +0.5 2,709,471 - Pf1N1B4_2391 0.64 +0.1 2,709,471 - Pf1N1B4_2391 0.64 +1.0 2,709,527 + Pf1N1B4_2391 0.69 +0.3 2,709,527 + Pf1N1B4_2391 0.69 +0.9 2,709,527 + Pf1N1B4_2391 0.69 +1.7 2,709,528 - Pf1N1B4_2391 0.69 +1.6 2,709,528 - Pf1N1B4_2391 0.69 +0.5 2,709,528 - Pf1N1B4_2391 0.69 +1.8 2,709,528 - Pf1N1B4_2391 0.69 -1.3 2,709,528 - Pf1N1B4_2391 0.69 -0.3 2,709,528 - Pf1N1B4_2391 0.69 -1.3 2,709,528 - Pf1N1B4_2391 0.69 -0.5 2,709,528 - Pf1N1B4_2391 0.69 -0.5 2,709,578 + Pf1N1B4_2391 0.73 -0.4 2,709,579 - Pf1N1B4_2391 0.73 +2.6 2,709,579 - Pf1N1B4_2391 0.73 -0.1 2,709,687 + Pf1N1B4_2391 0.83 -1.0 2,709,770 + Pf1N1B4_2391 0.90 -0.2 2,709,770 + Pf1N1B4_2391 0.90 +0.8 2,709,771 - Pf1N1B4_2391 0.90 +1.4 2,709,896 + -0.0 2,709,896 + +0.6 2,709,897 - -1.5 2,709,897 - +1.9 2,709,897 - +0.8 2,709,950 + +1.9 2,709,950 + -0.7 2,709,953 + +0.3 2,709,953 + +1.3 2,709,953 + -2.3 2,709,953 + -1.3 2,709,954 - -1.3 2,709,954 - -0.2 2,710,028 + -1.0 2,710,029 - -1.5 2,710,045 + -0.8 2,710,045 + +0.4 2,710,045 + -0.2 2,710,045 + +0.3 2,710,045 + +0.3 2,710,045 + -0.0 2,710,045 + +0.1 2,710,045 + -0.4 2,710,045 + -1.9 2,710,045 + +1.3 2,710,045 + +0.3 2,710,045 + -0.5 2,710,045 + +0.3 2,710,046 - -1.9 2,710,046 - -0.4 2,710,046 - -1.8 2,710,046 - -0.0 2,710,175 + Pf1N1B4_2392 0.34 +1.1 2,710,175 + Pf1N1B4_2392 0.34 +0.3 2,710,175 + Pf1N1B4_2392 0.34 -0.6 2,710,176 - Pf1N1B4_2392 0.35 +1.6 2,710,386 + Pf1N1B4_2392 0.81 +0.7 2,710,386 + Pf1N1B4_2392 0.81 +0.5 2,710,387 - Pf1N1B4_2392 0.82 +1.9 2,710,387 - +0.3 2,710,513 + +2.7 2,710,513 + +0.6 2,710,513 + +1.1 2,710,513 + +1.3 2,710,513 + -0.0 2,710,514 - -0.8 2,710,514 - -2.8 2,710,514 - -0.7 2,710,517 + -0.7 2,710,517 + +0.4 2,710,517 + -2.0
Or see this region's nucleotide sequence