Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1769 and Pf1N1B4_1770 are separated by 268 nucleotides Pf1N1B4_1770 and Pf1N1B4_1771 overlap by 1 nucleotides Pf1N1B4_1771 and Pf1N1B4_1772 are separated by 361 nucleotides
Pf1N1B4_1769 - Glutamate--cysteine ligase (EC 6.3.2.2), at 2,021,931 to 2,023,514
_1769
Pf1N1B4_1770 - Uncharacterized protein, possibly involved in aromatic compounds catabolism, at 2,023,783 to 2,024,166
_1770
Pf1N1B4_1771 - Transcription accessory protein (S1 RNA-binding domain), at 2,024,166 to 2,026,490
_1771
Pf1N1B4_1772 - Two-component system response regulator OmpR, at 2,026,852 to 2,027,592
_1772
Position (kb)
2024
2025
2026
2027 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2023.568 kb on + strand at 2023.568 kb on + strand at 2023.569 kb on - strand at 2023.582 kb on + strand at 2023.682 kb on + strand at 2023.697 kb on - strand at 2023.697 kb on - strand at 2023.697 kb on - strand at 2023.697 kb on - strand at 2023.749 kb on - strand at 2023.776 kb on + strand at 2023.776 kb on + strand at 2023.777 kb on - strand at 2023.777 kb on - strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.781 kb on - strand at 2023.781 kb on - strand at 2023.781 kb on - strand at 2023.781 kb on - strand at 2023.781 kb on - strand at 2023.784 kb on + strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.464 kb on - strand, within Pf1N1B4_1771 at 2024.464 kb on - strand, within Pf1N1B4_1771 at 2024.464 kb on - strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.684 kb on - strand, within Pf1N1B4_1771 at 2024.684 kb on - strand, within Pf1N1B4_1771 at 2024.684 kb on - strand, within Pf1N1B4_1771 at 2024.829 kb on + strand, within Pf1N1B4_1771 at 2024.829 kb on + strand, within Pf1N1B4_1771 at 2024.829 kb on + strand, within Pf1N1B4_1771 at 2025.089 kb on - strand, within Pf1N1B4_1771 at 2025.229 kb on + strand, within Pf1N1B4_1771 at 2025.229 kb on + strand, within Pf1N1B4_1771 at 2025.229 kb on + strand, within Pf1N1B4_1771 at 2025.230 kb on - strand, within Pf1N1B4_1771 at 2025.640 kb on + strand, within Pf1N1B4_1771 at 2025.640 kb on + strand, within Pf1N1B4_1771 at 2025.640 kb on + strand, within Pf1N1B4_1771 at 2025.640 kb on + strand, within Pf1N1B4_1771 at 2025.640 kb on + strand, within Pf1N1B4_1771 at 2025.641 kb on - strand, within Pf1N1B4_1771 at 2025.641 kb on - strand, within Pf1N1B4_1771 at 2025.641 kb on - strand, within Pf1N1B4_1771 at 2025.641 kb on - strand, within Pf1N1B4_1771 at 2025.641 kb on - strand, within Pf1N1B4_1771 at 2025.949 kb on + strand, within Pf1N1B4_1771 at 2025.949 kb on + strand, within Pf1N1B4_1771 at 2025.949 kb on + strand, within Pf1N1B4_1771 at 2025.950 kb on - strand, within Pf1N1B4_1771 at 2025.950 kb on - strand at 2025.953 kb on + strand, within Pf1N1B4_1771 at 2025.953 kb on + strand, within Pf1N1B4_1771 at 2025.954 kb on - strand, within Pf1N1B4_1771 at 2026.006 kb on + strand at 2026.006 kb on + strand, within Pf1N1B4_1771 at 2026.006 kb on + strand, within Pf1N1B4_1771 at 2026.006 kb on + strand, within Pf1N1B4_1771 at 2026.006 kb on + strand at 2026.006 kb on + strand, within Pf1N1B4_1771 at 2026.006 kb on + strand, within Pf1N1B4_1771 at 2026.007 kb on - strand, within Pf1N1B4_1771 at 2026.007 kb on - strand at 2026.007 kb on - strand, within Pf1N1B4_1771 at 2026.007 kb on - strand, within Pf1N1B4_1771 at 2026.007 kb on - strand, within Pf1N1B4_1771 at 2026.007 kb on - strand, within Pf1N1B4_1771 at 2026.007 kb on - strand, within Pf1N1B4_1771 at 2026.007 kb on - strand, within Pf1N1B4_1771 at 2026.177 kb on + strand, within Pf1N1B4_1771 at 2026.178 kb on - strand, within Pf1N1B4_1771 at 2026.178 kb on - strand, within Pf1N1B4_1771 at 2026.178 kb on - strand, within Pf1N1B4_1771 at 2026.186 kb on + strand, within Pf1N1B4_1771 at 2026.187 kb on - strand, within Pf1N1B4_1771 at 2026.187 kb on - strand, within Pf1N1B4_1771 at 2026.187 kb on - strand, within Pf1N1B4_1771 at 2026.187 kb on - strand, within Pf1N1B4_1771 at 2026.187 kb on - strand, within Pf1N1B4_1771 at 2026.312 kb on + strand at 2026.400 kb on + strand at 2026.401 kb on - strand at 2026.450 kb on - strand at 2026.536 kb on + strand at 2026.536 kb on + strand at 2026.536 kb on + strand at 2026.536 kb on + strand at 2026.537 kb on - strand at 2026.625 kb on - strand at 2026.625 kb on - strand at 2026.625 kb on - strand at 2026.658 kb on + strand at 2026.658 kb on + strand at 2026.658 kb on + strand at 2026.658 kb on + strand at 2026.658 kb on + strand at 2026.658 kb on + strand at 2026.701 kb on + strand at 2026.701 kb on + strand at 2026.701 kb on + strand at 2026.701 kb on + strand at 2026.701 kb on + strand at 2026.701 kb on + strand at 2026.701 kb on + strand at 2026.701 kb on + strand at 2026.702 kb on - strand at 2026.702 kb on - strand at 2026.703 kb on + strand at 2026.703 kb on + strand at 2026.703 kb on + strand at 2026.704 kb on - strand at 2026.704 kb on - strand at 2026.741 kb on + strand at 2026.803 kb on + strand at 2026.803 kb on + strand at 2026.803 kb on + strand at 2026.803 kb on + strand at 2026.803 kb on + strand at 2026.815 kb on + strand at 2026.815 kb on + strand at 2026.822 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 2,023,568 + -1.4 2,023,568 + -2.1 2,023,569 - -1.6 2,023,582 + -0.2 2,023,682 + -1.0 2,023,697 - +1.4 2,023,697 - -1.4 2,023,697 - -0.0 2,023,697 - -2.0 2,023,749 - -0.7 2,023,776 + +0.5 2,023,776 + +0.8 2,023,777 - +0.2 2,023,777 - -2.1 2,023,780 + +0.4 2,023,780 + -0.4 2,023,780 + -1.0 2,023,780 + +0.4 2,023,780 + -0.2 2,023,780 + -0.8 2,023,781 - +0.1 2,023,781 - +0.6 2,023,781 - -0.4 2,023,781 - +1.4 2,023,781 - +0.0 2,023,784 + +0.4 2,023,785 - +0.0 2,023,785 - -1.0 2,023,785 - +2.4 2,023,785 - +1.4 2,023,785 - -1.6 2,023,785 - +0.2 2,023,925 + Pf1N1B4_1770 0.37 +1.4 2,023,925 + Pf1N1B4_1770 0.37 -0.4 2,023,925 + Pf1N1B4_1770 0.37 -1.8 2,023,925 + Pf1N1B4_1770 0.37 +0.0 2,023,925 + Pf1N1B4_1770 0.37 -0.8 2,023,925 + Pf1N1B4_1770 0.37 -2.0 2,023,925 + +0.4 2,023,925 + Pf1N1B4_1770 0.37 -0.2 2,023,926 - Pf1N1B4_1770 0.37 +0.4 2,023,926 - Pf1N1B4_1770 0.37 +0.0 2,023,926 - Pf1N1B4_1770 0.37 -0.0 2,023,926 - Pf1N1B4_1770 0.37 -1.0 2,023,926 - Pf1N1B4_1770 0.37 +1.4 2,023,926 - Pf1N1B4_1770 0.37 +0.4 2,023,926 - Pf1N1B4_1770 0.37 +0.3 2,024,459 + Pf1N1B4_1771 0.13 +0.4 2,024,459 + Pf1N1B4_1771 0.13 +0.0 2,024,459 + Pf1N1B4_1771 0.13 -0.2 2,024,459 + Pf1N1B4_1771 0.13 +0.8 2,024,459 + Pf1N1B4_1771 0.13 -0.6 2,024,459 + Pf1N1B4_1771 0.13 +1.7 2,024,459 + Pf1N1B4_1771 0.13 +1.8 2,024,459 + Pf1N1B4_1771 0.13 -1.0 2,024,459 + Pf1N1B4_1771 0.13 -1.6 2,024,459 + Pf1N1B4_1771 0.13 -1.0 2,024,459 + Pf1N1B4_1771 0.13 -1.2 2,024,459 + Pf1N1B4_1771 0.13 +0.6 2,024,459 + Pf1N1B4_1771 0.13 -2.4 2,024,459 + Pf1N1B4_1771 0.13 -0.2 2,024,460 - Pf1N1B4_1771 0.13 -0.2 2,024,460 - Pf1N1B4_1771 0.13 -1.0 2,024,460 - Pf1N1B4_1771 0.13 +0.1 2,024,460 - Pf1N1B4_1771 0.13 -0.2 2,024,460 - Pf1N1B4_1771 0.13 -0.6 2,024,460 - Pf1N1B4_1771 0.13 -0.8 2,024,460 - Pf1N1B4_1771 0.13 -1.0 2,024,460 - Pf1N1B4_1771 0.13 -0.8 2,024,460 - Pf1N1B4_1771 0.13 -0.4 2,024,460 - +0.8 2,024,460 - Pf1N1B4_1771 0.13 +1.1 2,024,460 - +1.4 2,024,460 - Pf1N1B4_1771 0.13 -0.4 2,024,460 - Pf1N1B4_1771 0.13 +1.4 2,024,460 - Pf1N1B4_1771 0.13 -0.4 2,024,460 - Pf1N1B4_1771 0.13 -1.4 2,024,460 - +1.4 2,024,463 + Pf1N1B4_1771 0.13 -0.8 2,024,463 + Pf1N1B4_1771 0.13 -1.8 2,024,463 + Pf1N1B4_1771 0.13 +0.5 2,024,463 + Pf1N1B4_1771 0.13 -0.0 2,024,463 + Pf1N1B4_1771 0.13 +0.1 2,024,464 - Pf1N1B4_1771 0.13 -1.4 2,024,464 - Pf1N1B4_1771 0.13 +1.0 2,024,464 - Pf1N1B4_1771 0.13 -0.6 2,024,683 + Pf1N1B4_1771 0.22 +0.6 2,024,683 + Pf1N1B4_1771 0.22 -1.6 2,024,683 + Pf1N1B4_1771 0.22 -0.5 2,024,683 + Pf1N1B4_1771 0.22 +0.4 2,024,683 + Pf1N1B4_1771 0.22 +2.0 2,024,684 - Pf1N1B4_1771 0.22 -0.8 2,024,684 - Pf1N1B4_1771 0.22 +1.0 2,024,684 - Pf1N1B4_1771 0.22 -1.4 2,024,829 + Pf1N1B4_1771 0.29 -0.5 2,024,829 + Pf1N1B4_1771 0.29 -0.2 2,024,829 + Pf1N1B4_1771 0.29 -0.6 2,025,089 - Pf1N1B4_1771 0.40 -0.2 2,025,229 + Pf1N1B4_1771 0.46 +0.4 2,025,229 + Pf1N1B4_1771 0.46 -0.6 2,025,229 + Pf1N1B4_1771 0.46 +0.2 2,025,230 - Pf1N1B4_1771 0.46 -1.4 2,025,640 + Pf1N1B4_1771 0.63 +0.4 2,025,640 + Pf1N1B4_1771 0.63 +0.9 2,025,640 + Pf1N1B4_1771 0.63 -1.4 2,025,640 + Pf1N1B4_1771 0.63 -0.2 2,025,640 + Pf1N1B4_1771 0.63 -0.1 2,025,641 - Pf1N1B4_1771 0.63 +0.1 2,025,641 - Pf1N1B4_1771 0.63 +0.3 2,025,641 - Pf1N1B4_1771 0.63 +0.2 2,025,641 - Pf1N1B4_1771 0.63 -0.8 2,025,641 - Pf1N1B4_1771 0.63 +0.5 2,025,949 + Pf1N1B4_1771 0.77 -0.1 2,025,949 + Pf1N1B4_1771 0.77 +1.0 2,025,949 + Pf1N1B4_1771 0.77 -2.2 2,025,950 - Pf1N1B4_1771 0.77 -0.0 2,025,950 - +1.4 2,025,953 + Pf1N1B4_1771 0.77 +1.4 2,025,953 + Pf1N1B4_1771 0.77 -1.0 2,025,954 - Pf1N1B4_1771 0.77 +0.1 2,026,006 + -1.0 2,026,006 + Pf1N1B4_1771 0.79 -0.2 2,026,006 + Pf1N1B4_1771 0.79 -1.0 2,026,006 + Pf1N1B4_1771 0.79 +0.6 2,026,006 + +1.4 2,026,006 + Pf1N1B4_1771 0.79 -2.5 2,026,006 + Pf1N1B4_1771 0.79 -1.1 2,026,007 - Pf1N1B4_1771 0.79 +0.4 2,026,007 - +0.4 2,026,007 - Pf1N1B4_1771 0.79 -0.5 2,026,007 - Pf1N1B4_1771 0.79 -1.1 2,026,007 - Pf1N1B4_1771 0.79 -0.6 2,026,007 - Pf1N1B4_1771 0.79 -0.6 2,026,007 - Pf1N1B4_1771 0.79 +1.4 2,026,007 - Pf1N1B4_1771 0.79 +1.0 2,026,177 + Pf1N1B4_1771 0.86 +0.7 2,026,178 - Pf1N1B4_1771 0.87 +0.2 2,026,178 - Pf1N1B4_1771 0.87 +0.0 2,026,178 - Pf1N1B4_1771 0.87 -1.8 2,026,186 + Pf1N1B4_1771 0.87 +0.0 2,026,187 - Pf1N1B4_1771 0.87 +0.4 2,026,187 - Pf1N1B4_1771 0.87 -0.2 2,026,187 - Pf1N1B4_1771 0.87 -1.9 2,026,187 - Pf1N1B4_1771 0.87 -1.0 2,026,187 - Pf1N1B4_1771 0.87 +0.8 2,026,312 + +1.0 2,026,400 + -1.4 2,026,401 - +0.5 2,026,450 - +0.4 2,026,536 + -0.0 2,026,536 + +0.4 2,026,536 + -2.4 2,026,536 + -2.1 2,026,537 - +0.2 2,026,625 - +1.9 2,026,625 - -0.5 2,026,625 - +1.0 2,026,658 + -0.4 2,026,658 + +0.8 2,026,658 + +0.5 2,026,658 + +0.4 2,026,658 + -2.0 2,026,658 + +0.4 2,026,701 + -0.8 2,026,701 + -2.0 2,026,701 + +1.4 2,026,701 + -0.1 2,026,701 + -0.2 2,026,701 + -0.4 2,026,701 + -1.3 2,026,701 + -0.7 2,026,702 - -0.4 2,026,702 - -1.4 2,026,703 + -0.6 2,026,703 + -1.4 2,026,703 + +0.6 2,026,704 - -0.8 2,026,704 - -0.6 2,026,741 + -0.4 2,026,803 + -0.2 2,026,803 + -0.2 2,026,803 + -0.4 2,026,803 + -0.6 2,026,803 + +1.4 2,026,815 + +0.9 2,026,815 + -0.3 2,026,822 + -1.2
Or see this region's nucleotide sequence