Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1732 and Pf1N1B4_1733 are separated by 148 nucleotides Pf1N1B4_1733 and Pf1N1B4_1734 are separated by 180 nucleotides
Pf1N1B4_1732 - response regulator, at 1,979,828 to 1,981,042
_1732
Pf1N1B4_1733 - 5-aminovalerate aminotransferase (EC 2.6.1.48) / Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 1,981,191 to 1,982,468
_1733
Pf1N1B4_1734 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 1,982,649 to 1,984,091
_1734
Position (kb)
1981
1982
1983 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1980.192 kb on + strand, within Pf1N1B4_1732 at 1980.193 kb on - strand, within Pf1N1B4_1732 at 1980.240 kb on + strand, within Pf1N1B4_1732 at 1980.283 kb on - strand, within Pf1N1B4_1732 at 1980.451 kb on + strand, within Pf1N1B4_1732 at 1980.451 kb on + strand, within Pf1N1B4_1732 at 1980.452 kb on - strand, within Pf1N1B4_1732 at 1980.452 kb on - strand, within Pf1N1B4_1732 at 1980.517 kb on + strand, within Pf1N1B4_1732 at 1980.517 kb on + strand, within Pf1N1B4_1732 at 1980.518 kb on - strand, within Pf1N1B4_1732 at 1980.518 kb on - strand, within Pf1N1B4_1732 at 1980.588 kb on + strand, within Pf1N1B4_1732 at 1980.589 kb on - strand, within Pf1N1B4_1732 at 1980.589 kb on - strand, within Pf1N1B4_1732 at 1980.589 kb on - strand, within Pf1N1B4_1732 at 1980.589 kb on - strand, within Pf1N1B4_1732 at 1980.711 kb on + strand, within Pf1N1B4_1732 at 1980.797 kb on - strand, within Pf1N1B4_1732 at 1980.797 kb on - strand, within Pf1N1B4_1732 at 1980.797 kb on - strand, within Pf1N1B4_1732 at 1980.802 kb on + strand, within Pf1N1B4_1732 at 1980.802 kb on + strand, within Pf1N1B4_1732 at 1980.876 kb on + strand, within Pf1N1B4_1732 at 1980.876 kb on + strand, within Pf1N1B4_1732 at 1980.877 kb on - strand, within Pf1N1B4_1732 at 1980.877 kb on - strand, within Pf1N1B4_1732 at 1981.025 kb on + strand at 1981.072 kb on + strand at 1981.073 kb on - strand at 1981.073 kb on - strand at 1981.093 kb on + strand at 1981.094 kb on - strand at 1981.128 kb on + strand at 1981.128 kb on + strand at 1981.128 kb on + strand at 1981.129 kb on - strand at 1981.270 kb on - strand at 1981.289 kb on - strand at 1981.289 kb on - strand at 1981.311 kb on - strand at 1981.441 kb on + strand, within Pf1N1B4_1733 at 1981.573 kb on + strand, within Pf1N1B4_1733 at 1981.573 kb on + strand, within Pf1N1B4_1733 at 1981.573 kb on + strand, within Pf1N1B4_1733 at 1981.574 kb on - strand, within Pf1N1B4_1733 at 1981.574 kb on - strand, within Pf1N1B4_1733 at 1981.574 kb on - strand, within Pf1N1B4_1733 at 1981.574 kb on - strand, within Pf1N1B4_1733 at 1981.574 kb on - strand, within Pf1N1B4_1733 at 1981.601 kb on + strand, within Pf1N1B4_1733 at 1981.612 kb on + strand, within Pf1N1B4_1733 at 1981.612 kb on + strand, within Pf1N1B4_1733 at 1981.613 kb on - strand, within Pf1N1B4_1733 at 1981.613 kb on - strand, within Pf1N1B4_1733 at 1981.613 kb on - strand, within Pf1N1B4_1733 at 1981.613 kb on - strand, within Pf1N1B4_1733 at 1981.613 kb on - strand, within Pf1N1B4_1733 at 1981.613 kb on - strand, within Pf1N1B4_1733 at 1981.743 kb on + strand, within Pf1N1B4_1733 at 1981.743 kb on + strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.761 kb on + strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.823 kb on - strand, within Pf1N1B4_1733 at 1981.823 kb on - strand, within Pf1N1B4_1733 at 1982.015 kb on + strand, within Pf1N1B4_1733 at 1982.053 kb on + strand, within Pf1N1B4_1733 at 1982.053 kb on + strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.218 kb on + strand, within Pf1N1B4_1733 at 1982.219 kb on - strand, within Pf1N1B4_1733 at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.531 kb on + strand at 1982.532 kb on - strand at 1982.532 kb on - strand at 1982.532 kb on - strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.651 kb on - strand at 1982.651 kb on - strand at 1982.651 kb on - strand at 1982.668 kb on + strand at 1982.669 kb on - strand at 1982.669 kb on - strand at 1982.669 kb on - strand at 1982.669 kb on - strand at 1982.683 kb on + strand at 1982.683 kb on + strand at 1982.698 kb on + strand at 1982.699 kb on - strand at 1982.699 kb on - strand at 1982.795 kb on - strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.849 kb on - strand, within Pf1N1B4_1734 at 1982.849 kb on - strand, within Pf1N1B4_1734 at 1982.849 kb on - strand, within Pf1N1B4_1734 at 1983.187 kb on + strand, within Pf1N1B4_1734 at 1983.205 kb on + strand, within Pf1N1B4_1734 at 1983.206 kb on - strand, within Pf1N1B4_1734 at 1983.206 kb on - strand, within Pf1N1B4_1734 at 1983.206 kb on - strand, within Pf1N1B4_1734
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 1,980,192 + Pf1N1B4_1732 0.30 +0.6 1,980,193 - Pf1N1B4_1732 0.30 +0.4 1,980,240 + Pf1N1B4_1732 0.34 -0.6 1,980,283 - Pf1N1B4_1732 0.37 -0.6 1,980,451 + Pf1N1B4_1732 0.51 -1.4 1,980,451 + Pf1N1B4_1732 0.51 +0.1 1,980,452 - Pf1N1B4_1732 0.51 -2.0 1,980,452 - Pf1N1B4_1732 0.51 -0.7 1,980,517 + Pf1N1B4_1732 0.57 +0.9 1,980,517 + Pf1N1B4_1732 0.57 -0.6 1,980,518 - Pf1N1B4_1732 0.57 -0.6 1,980,518 - Pf1N1B4_1732 0.57 -0.4 1,980,588 + Pf1N1B4_1732 0.63 -1.0 1,980,589 - Pf1N1B4_1732 0.63 -0.2 1,980,589 - Pf1N1B4_1732 0.63 -1.4 1,980,589 - Pf1N1B4_1732 0.63 +1.1 1,980,589 - Pf1N1B4_1732 0.63 -0.8 1,980,711 + Pf1N1B4_1732 0.73 -1.1 1,980,797 - Pf1N1B4_1732 0.80 +0.1 1,980,797 - Pf1N1B4_1732 0.80 -1.0 1,980,797 - Pf1N1B4_1732 0.80 -0.2 1,980,802 + Pf1N1B4_1732 0.80 -0.1 1,980,802 + Pf1N1B4_1732 0.80 +0.9 1,980,876 + Pf1N1B4_1732 0.86 -0.6 1,980,876 + Pf1N1B4_1732 0.86 -0.2 1,980,877 - Pf1N1B4_1732 0.86 -0.2 1,980,877 - Pf1N1B4_1732 0.86 -0.8 1,981,025 + -2.0 1,981,072 + +1.0 1,981,073 - -0.2 1,981,073 - +0.4 1,981,093 + -0.4 1,981,094 - +0.4 1,981,128 + -0.2 1,981,128 + +0.1 1,981,128 + +1.4 1,981,129 - +1.4 1,981,270 - -0.6 1,981,289 - -0.2 1,981,289 - -0.6 1,981,311 - -1.6 1,981,441 + Pf1N1B4_1733 0.20 -1.4 1,981,573 + Pf1N1B4_1733 0.30 -1.2 1,981,573 + Pf1N1B4_1733 0.30 -0.4 1,981,573 + Pf1N1B4_1733 0.30 -2.2 1,981,574 - Pf1N1B4_1733 0.30 -2.4 1,981,574 - Pf1N1B4_1733 0.30 +0.4 1,981,574 - Pf1N1B4_1733 0.30 -2.6 1,981,574 - Pf1N1B4_1733 0.30 -1.4 1,981,574 - Pf1N1B4_1733 0.30 +0.8 1,981,601 + Pf1N1B4_1733 0.32 -1.1 1,981,612 + Pf1N1B4_1733 0.33 -1.2 1,981,612 + Pf1N1B4_1733 0.33 -0.6 1,981,613 - Pf1N1B4_1733 0.33 -1.7 1,981,613 - Pf1N1B4_1733 0.33 -1.4 1,981,613 - Pf1N1B4_1733 0.33 -0.4 1,981,613 - Pf1N1B4_1733 0.33 +0.0 1,981,613 - Pf1N1B4_1733 0.33 -0.2 1,981,613 - Pf1N1B4_1733 0.33 -0.4 1,981,743 + Pf1N1B4_1733 0.43 -0.8 1,981,743 + Pf1N1B4_1733 0.43 -0.5 1,981,744 - Pf1N1B4_1733 0.43 -0.7 1,981,744 - Pf1N1B4_1733 0.43 +0.0 1,981,744 - Pf1N1B4_1733 0.43 +0.9 1,981,744 - Pf1N1B4_1733 0.43 -1.5 1,981,761 + Pf1N1B4_1733 0.45 -0.9 1,981,762 - Pf1N1B4_1733 0.45 -0.4 1,981,762 - Pf1N1B4_1733 0.45 -0.4 1,981,762 - Pf1N1B4_1733 0.45 +0.4 1,981,762 - Pf1N1B4_1733 0.45 +0.2 1,981,762 - Pf1N1B4_1733 0.45 +0.7 1,981,762 - Pf1N1B4_1733 0.45 -1.8 1,981,762 - Pf1N1B4_1733 0.45 -0.9 1,981,823 - Pf1N1B4_1733 0.49 +0.4 1,981,823 - Pf1N1B4_1733 0.49 -0.2 1,982,015 + Pf1N1B4_1733 0.64 -0.2 1,982,053 + Pf1N1B4_1733 0.67 -0.8 1,982,053 + Pf1N1B4_1733 0.67 -0.0 1,982,210 - Pf1N1B4_1733 0.80 +2.2 1,982,210 - Pf1N1B4_1733 0.80 -0.2 1,982,210 - Pf1N1B4_1733 0.80 -0.2 1,982,210 - Pf1N1B4_1733 0.80 +0.1 1,982,218 + Pf1N1B4_1733 0.80 -0.2 1,982,219 - Pf1N1B4_1733 0.80 -0.4 1,982,468 + +1.1 1,982,468 + +1.4 1,982,468 + -0.8 1,982,468 + +0.4 1,982,468 + -0.8 1,982,468 + -2.1 1,982,469 - -0.2 1,982,469 - +0.4 1,982,469 - -0.8 1,982,469 - -1.2 1,982,469 - +1.4 1,982,469 - +0.0 1,982,531 + -0.6 1,982,532 - +0.1 1,982,532 - -1.2 1,982,532 - +0.0 1,982,650 + +1.8 1,982,650 + +2.4 1,982,650 + +0.8 1,982,650 + +0.4 1,982,650 + -2.8 1,982,650 + -1.3 1,982,650 + -1.8 1,982,651 - -1.8 1,982,651 - -0.6 1,982,651 - +0.1 1,982,668 + +0.8 1,982,669 - +0.1 1,982,669 - -1.1 1,982,669 - -1.6 1,982,669 - -0.1 1,982,683 + +1.9 1,982,683 + -2.2 1,982,698 + -0.8 1,982,699 - +0.5 1,982,699 - -1.2 1,982,795 - Pf1N1B4_1734 0.10 -2.9 1,982,842 + Pf1N1B4_1734 0.13 +0.6 1,982,842 + Pf1N1B4_1734 0.13 +0.4 1,982,842 + Pf1N1B4_1734 0.13 +0.6 1,982,842 + Pf1N1B4_1734 0.13 -0.6 1,982,842 + Pf1N1B4_1734 0.13 -1.0 1,982,843 - Pf1N1B4_1734 0.13 +2.6 1,982,843 - Pf1N1B4_1734 0.13 +0.2 1,982,843 - Pf1N1B4_1734 0.13 -0.2 1,982,843 - Pf1N1B4_1734 0.13 +0.7 1,982,843 - Pf1N1B4_1734 0.13 -1.2 1,982,843 - Pf1N1B4_1734 0.13 +0.8 1,982,843 - Pf1N1B4_1734 0.13 -1.6 1,982,843 - Pf1N1B4_1734 0.13 +1.4 1,982,843 - Pf1N1B4_1734 0.13 +0.8 1,982,843 - Pf1N1B4_1734 0.13 -0.5 1,982,843 - Pf1N1B4_1734 0.13 -0.2 1,982,843 - Pf1N1B4_1734 0.13 -0.9 1,982,848 + Pf1N1B4_1734 0.14 +0.5 1,982,848 + Pf1N1B4_1734 0.14 -0.6 1,982,848 + Pf1N1B4_1734 0.14 -1.0 1,982,848 + Pf1N1B4_1734 0.14 -0.2 1,982,848 + Pf1N1B4_1734 0.14 +2.4 1,982,848 + Pf1N1B4_1734 0.14 +1.4 1,982,849 - Pf1N1B4_1734 0.14 +0.4 1,982,849 - Pf1N1B4_1734 0.14 -0.2 1,982,849 - Pf1N1B4_1734 0.14 -1.2 1,983,187 + Pf1N1B4_1734 0.37 -1.8 1,983,205 + Pf1N1B4_1734 0.39 +2.4 1,983,206 - Pf1N1B4_1734 0.39 -0.9 1,983,206 - Pf1N1B4_1734 0.39 -2.2 1,983,206 - Pf1N1B4_1734 0.39 -0.4
Or see this region's nucleotide sequence