Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1573 and Pf1N1B4_1574 are separated by 351 nucleotides Pf1N1B4_1574 and Pf1N1B4_1575 are separated by 50 nucleotides
Pf1N1B4_1573 - Glucans biosynthesis protein G precursor, at 1,811,475 to 1,813,250
_1573
Pf1N1B4_1574 - D-tyrosyl-tRNA(Tyr) deacylase (EC 3.6.1.n1), at 1,813,602 to 1,813,985
_1574
Pf1N1B4_1575 - Proline iminopeptidase (EC 3.4.11.5), at 1,814,036 to 1,815,007
_1575
Position (kb)
1813
1814 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1812.769 kb on + strand, within Pf1N1B4_1573 at 1812.769 kb on + strand, within Pf1N1B4_1573 at 1812.769 kb on + strand, within Pf1N1B4_1573 at 1812.770 kb on - strand, within Pf1N1B4_1573 at 1812.869 kb on + strand, within Pf1N1B4_1573 at 1812.870 kb on - strand, within Pf1N1B4_1573 at 1812.870 kb on - strand, within Pf1N1B4_1573 at 1812.914 kb on - strand, within Pf1N1B4_1573 at 1813.218 kb on + strand at 1813.263 kb on - strand at 1813.449 kb on + strand at 1813.450 kb on - strand at 1813.453 kb on + strand at 1813.453 kb on + strand at 1813.454 kb on - strand at 1813.454 kb on - strand at 1813.454 kb on - strand at 1813.454 kb on - strand at 1813.454 kb on - strand at 1813.454 kb on - strand at 1813.498 kb on + strand at 1813.502 kb on + strand at 1813.503 kb on - strand at 1813.503 kb on - strand at 1813.515 kb on + strand at 1813.540 kb on - strand at 1813.540 kb on - strand at 1813.540 kb on - strand at 1813.581 kb on - strand at 1813.581 kb on - strand at 1813.655 kb on + strand, within Pf1N1B4_1574 at 1813.671 kb on - strand, within Pf1N1B4_1574 at 1813.706 kb on + strand, within Pf1N1B4_1574 at 1813.706 kb on + strand, within Pf1N1B4_1574 at 1813.713 kb on + strand, within Pf1N1B4_1574 at 1813.713 kb on + strand, within Pf1N1B4_1574 at 1813.714 kb on - strand at 1813.714 kb on - strand at 1813.714 kb on - strand, within Pf1N1B4_1574 at 1813.714 kb on - strand at 1813.714 kb on - strand, within Pf1N1B4_1574 at 1813.714 kb on - strand, within Pf1N1B4_1574 at 1813.714 kb on - strand, within Pf1N1B4_1574 at 1813.834 kb on - strand, within Pf1N1B4_1574 at 1813.889 kb on + strand, within Pf1N1B4_1574 at 1813.889 kb on + strand, within Pf1N1B4_1574 at 1813.890 kb on - strand, within Pf1N1B4_1574 at 1813.890 kb on - strand, within Pf1N1B4_1574 at 1813.890 kb on - strand, within Pf1N1B4_1574 at 1813.890 kb on - strand, within Pf1N1B4_1574 at 1813.898 kb on + strand, within Pf1N1B4_1574 at 1813.898 kb on + strand, within Pf1N1B4_1574 at 1813.972 kb on - strand at 1813.972 kb on - strand at 1813.972 kb on - strand at 1813.972 kb on - strand at 1814.025 kb on + strand at 1814.025 kb on + strand at 1814.025 kb on + strand at 1814.026 kb on - strand at 1814.108 kb on - strand at 1814.108 kb on - strand at 1814.110 kb on + strand at 1814.110 kb on + strand at 1814.110 kb on + strand at 1814.110 kb on + strand at 1814.110 kb on + strand at 1814.111 kb on - strand at 1814.111 kb on - strand at 1814.111 kb on - strand at 1814.111 kb on - strand at 1814.111 kb on - strand at 1814.111 kb on - strand at 1814.310 kb on + strand, within Pf1N1B4_1575 at 1814.310 kb on + strand, within Pf1N1B4_1575 at 1814.310 kb on + strand, within Pf1N1B4_1575 at 1814.310 kb on + strand, within Pf1N1B4_1575 at 1814.310 kb on + strand, within Pf1N1B4_1575 at 1814.310 kb on + strand, within Pf1N1B4_1575 at 1814.310 kb on + strand, within Pf1N1B4_1575 at 1814.311 kb on - strand, within Pf1N1B4_1575 at 1814.311 kb on - strand, within Pf1N1B4_1575 at 1814.311 kb on - strand, within Pf1N1B4_1575 at 1814.311 kb on - strand, within Pf1N1B4_1575 at 1814.486 kb on + strand, within Pf1N1B4_1575 at 1814.523 kb on + strand, within Pf1N1B4_1575 at 1814.523 kb on + strand, within Pf1N1B4_1575 at 1814.523 kb on + strand, within Pf1N1B4_1575 at 1814.523 kb on + strand, within Pf1N1B4_1575 at 1814.523 kb on + strand, within Pf1N1B4_1575 at 1814.524 kb on - strand, within Pf1N1B4_1575 at 1814.524 kb on - strand, within Pf1N1B4_1575 at 1814.524 kb on - strand, within Pf1N1B4_1575 at 1814.568 kb on + strand, within Pf1N1B4_1575 at 1814.568 kb on + strand, within Pf1N1B4_1575 at 1814.569 kb on - strand, within Pf1N1B4_1575 at 1814.653 kb on - strand, within Pf1N1B4_1575 at 1814.758 kb on + strand, within Pf1N1B4_1575 at 1814.934 kb on + strand at 1814.934 kb on + strand at 1814.935 kb on + strand at 1814.935 kb on - strand at 1814.935 kb on - strand at 1814.935 kb on - strand at 1814.935 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 1,812,769 + Pf1N1B4_1573 0.73 -0.7 1,812,769 + Pf1N1B4_1573 0.73 +0.4 1,812,769 + Pf1N1B4_1573 0.73 -0.7 1,812,770 - Pf1N1B4_1573 0.73 +0.3 1,812,869 + Pf1N1B4_1573 0.78 -0.5 1,812,870 - Pf1N1B4_1573 0.79 -0.3 1,812,870 - Pf1N1B4_1573 0.79 +0.0 1,812,914 - Pf1N1B4_1573 0.81 -0.5 1,813,218 + -0.7 1,813,263 - -0.0 1,813,449 + +1.1 1,813,450 - -2.6 1,813,453 + -0.7 1,813,453 + -0.4 1,813,454 - +0.1 1,813,454 - +1.5 1,813,454 - -1.1 1,813,454 - +1.9 1,813,454 - -1.3 1,813,454 - -1.5 1,813,498 + +0.5 1,813,502 + -0.0 1,813,503 - +0.9 1,813,503 - -0.6 1,813,515 + +0.4 1,813,540 - -1.0 1,813,540 - +1.1 1,813,540 - -0.5 1,813,581 - -1.0 1,813,581 - -1.4 1,813,655 + Pf1N1B4_1574 0.14 -0.1 1,813,671 - Pf1N1B4_1574 0.18 -2.0 1,813,706 + Pf1N1B4_1574 0.27 -1.0 1,813,706 + Pf1N1B4_1574 0.27 -2.3 1,813,713 + Pf1N1B4_1574 0.29 -0.3 1,813,713 + Pf1N1B4_1574 0.29 +0.9 1,813,714 - -0.3 1,813,714 - +1.3 1,813,714 - Pf1N1B4_1574 0.29 -1.0 1,813,714 - +1.3 1,813,714 - Pf1N1B4_1574 0.29 -0.6 1,813,714 - Pf1N1B4_1574 0.29 -2.0 1,813,714 - Pf1N1B4_1574 0.29 -1.1 1,813,834 - Pf1N1B4_1574 0.60 -0.6 1,813,889 + Pf1N1B4_1574 0.75 -0.3 1,813,889 + Pf1N1B4_1574 0.75 +1.3 1,813,890 - Pf1N1B4_1574 0.75 -2.5 1,813,890 - Pf1N1B4_1574 0.75 -0.7 1,813,890 - Pf1N1B4_1574 0.75 +0.6 1,813,890 - Pf1N1B4_1574 0.75 -0.4 1,813,898 + Pf1N1B4_1574 0.77 -2.2 1,813,898 + Pf1N1B4_1574 0.77 +0.5 1,813,972 - -1.3 1,813,972 - +0.1 1,813,972 - -0.2 1,813,972 - +1.3 1,814,025 + -0.2 1,814,025 + -2.0 1,814,025 + -1.3 1,814,026 - -1.3 1,814,108 - +0.9 1,814,108 - +0.3 1,814,110 + +0.7 1,814,110 + +0.3 1,814,110 + +0.3 1,814,110 + +1.8 1,814,110 + +0.0 1,814,111 - -1.1 1,814,111 - -1.0 1,814,111 - -1.7 1,814,111 - -1.3 1,814,111 - -0.3 1,814,111 - -1.0 1,814,310 + Pf1N1B4_1575 0.28 +1.5 1,814,310 + Pf1N1B4_1575 0.28 -1.3 1,814,310 + Pf1N1B4_1575 0.28 +0.3 1,814,310 + Pf1N1B4_1575 0.28 -0.1 1,814,310 + Pf1N1B4_1575 0.28 -2.1 1,814,310 + Pf1N1B4_1575 0.28 +1.6 1,814,310 + Pf1N1B4_1575 0.28 +1.5 1,814,311 - Pf1N1B4_1575 0.28 -2.5 1,814,311 - Pf1N1B4_1575 0.28 -1.3 1,814,311 - Pf1N1B4_1575 0.28 -2.4 1,814,311 - Pf1N1B4_1575 0.28 -0.0 1,814,486 + Pf1N1B4_1575 0.46 +0.2 1,814,523 + Pf1N1B4_1575 0.50 +0.9 1,814,523 + Pf1N1B4_1575 0.50 -0.2 1,814,523 + Pf1N1B4_1575 0.50 -1.3 1,814,523 + Pf1N1B4_1575 0.50 -1.9 1,814,523 + Pf1N1B4_1575 0.50 -0.3 1,814,524 - Pf1N1B4_1575 0.50 -0.7 1,814,524 - Pf1N1B4_1575 0.50 -0.0 1,814,524 - Pf1N1B4_1575 0.50 -0.5 1,814,568 + Pf1N1B4_1575 0.55 -0.3 1,814,568 + Pf1N1B4_1575 0.55 -1.2 1,814,569 - Pf1N1B4_1575 0.55 +1.3 1,814,653 - Pf1N1B4_1575 0.63 -0.0 1,814,758 + Pf1N1B4_1575 0.74 +1.3 1,814,934 + -1.3 1,814,934 + -0.1 1,814,935 + +1.3 1,814,935 - -0.4 1,814,935 - +1.0 1,814,935 - -0.7 1,814,935 - +1.3
Or see this region's nucleotide sequence