Experiment: Choline chloride 200 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1449 and Pf1N1B4_1450 are separated by 22 nucleotides Pf1N1B4_1450 and Pf1N1B4_1451 are separated by 103 nucleotides Pf1N1B4_1451 and Pf1N1B4_1452 are separated by 198 nucleotides
Pf1N1B4_1449 - 3-deoxy-D-manno-octulosonic-acid transferase (EC 2.-.-.-), at 1,662,505 to 1,663,785
_1449
Pf1N1B4_1450 - LysR family transcriptional regulator PA4989, at 1,663,808 to 1,664,698
_1450
Pf1N1B4_1451 - Ethidium bromide-methyl viologen resistance protein EmrE, at 1,664,802 to 1,665,134
_1451
Pf1N1B4_1452 - Oxidoreductase, FAD-binding, at 1,665,333 to 1,666,508
_1452
Position (kb)
1663
1664
1665 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1663.780 kb on - strand at 1663.962 kb on + strand, within Pf1N1B4_1450 at 1664.028 kb on + strand, within Pf1N1B4_1450 at 1664.028 kb on + strand, within Pf1N1B4_1450 at 1664.028 kb on + strand, within Pf1N1B4_1450 at 1664.028 kb on + strand, within Pf1N1B4_1450 at 1664.028 kb on + strand, within Pf1N1B4_1450 at 1664.028 kb on + strand, within Pf1N1B4_1450 at 1664.028 kb on + strand, within Pf1N1B4_1450 at 1664.028 kb on + strand, within Pf1N1B4_1450 at 1664.029 kb on - strand, within Pf1N1B4_1450 at 1664.029 kb on - strand, within Pf1N1B4_1450 at 1664.029 kb on - strand, within Pf1N1B4_1450 at 1664.029 kb on - strand, within Pf1N1B4_1450 at 1664.079 kb on + strand, within Pf1N1B4_1450 at 1664.183 kb on - strand, within Pf1N1B4_1450 at 1664.241 kb on + strand, within Pf1N1B4_1450 at 1664.241 kb on + strand, within Pf1N1B4_1450 at 1664.536 kb on + strand, within Pf1N1B4_1450 at 1664.536 kb on + strand, within Pf1N1B4_1450 at 1664.536 kb on + strand, within Pf1N1B4_1450 at 1664.537 kb on - strand, within Pf1N1B4_1450 at 1664.724 kb on - strand at 1664.756 kb on + strand at 1664.756 kb on + strand at 1664.756 kb on + strand at 1664.757 kb on - strand at 1664.757 kb on - strand at 1664.757 kb on - strand at 1664.757 kb on - strand at 1664.788 kb on - strand at 1664.811 kb on + strand at 1664.811 kb on + strand at 1664.811 kb on + strand at 1664.812 kb on - strand at 1664.812 kb on - strand at 1664.812 kb on - strand at 1664.812 kb on - strand at 1664.812 kb on - strand at 1664.812 kb on - strand at 1664.814 kb on + strand at 1664.814 kb on + strand at 1664.814 kb on + strand at 1664.814 kb on + strand at 1664.814 kb on + strand at 1664.814 kb on + strand at 1664.814 kb on + strand at 1664.814 kb on + strand at 1664.814 kb on + strand at 1664.814 kb on + strand at 1664.814 kb on + strand at 1664.814 kb on + strand at 1664.814 kb on + strand at 1664.814 kb on + strand at 1664.814 kb on + strand at 1664.815 kb on - strand at 1664.815 kb on - strand at 1664.815 kb on - strand at 1664.815 kb on - strand at 1664.815 kb on - strand at 1664.815 kb on - strand at 1664.815 kb on - strand at 1664.815 kb on - strand at 1664.815 kb on - strand at 1664.815 kb on - strand at 1664.815 kb on - strand at 1664.815 kb on - strand at 1664.815 kb on - strand at 1664.817 kb on + strand at 1664.817 kb on + strand at 1664.817 kb on + strand at 1664.817 kb on + strand at 1664.817 kb on + strand at 1664.817 kb on + strand at 1664.817 kb on + strand at 1664.817 kb on + strand at 1664.817 kb on + strand at 1664.818 kb on - strand at 1664.818 kb on - strand at 1664.818 kb on - strand at 1664.818 kb on - strand at 1664.818 kb on - strand at 1664.818 kb on - strand at 1664.879 kb on + strand, within Pf1N1B4_1451 at 1664.880 kb on - strand, within Pf1N1B4_1451 at 1665.033 kb on - strand, within Pf1N1B4_1451 at 1665.033 kb on - strand, within Pf1N1B4_1451 at 1665.049 kb on + strand, within Pf1N1B4_1451 at 1665.049 kb on + strand, within Pf1N1B4_1451 at 1665.049 kb on + strand, within Pf1N1B4_1451 at 1665.049 kb on + strand, within Pf1N1B4_1451 at 1665.049 kb on + strand, within Pf1N1B4_1451 at 1665.049 kb on + strand, within Pf1N1B4_1451 at 1665.050 kb on - strand, within Pf1N1B4_1451 at 1665.050 kb on - strand, within Pf1N1B4_1451 at 1665.085 kb on + strand, within Pf1N1B4_1451 at 1665.097 kb on + strand, within Pf1N1B4_1451 at 1665.097 kb on + strand, within Pf1N1B4_1451 at 1665.098 kb on - strand, within Pf1N1B4_1451 at 1665.132 kb on + strand at 1665.132 kb on + strand at 1665.296 kb on + strand at 1665.296 kb on + strand at 1665.296 kb on + strand at 1665.296 kb on + strand at 1665.297 kb on - strand at 1665.297 kb on - strand at 1665.298 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline chloride 200 mM remove 1,663,780 - -0.3 1,663,962 + Pf1N1B4_1450 0.17 -0.0 1,664,028 + Pf1N1B4_1450 0.25 -1.2 1,664,028 + Pf1N1B4_1450 0.25 +2.2 1,664,028 + Pf1N1B4_1450 0.25 -0.8 1,664,028 + Pf1N1B4_1450 0.25 +0.2 1,664,028 + Pf1N1B4_1450 0.25 +1.2 1,664,028 + Pf1N1B4_1450 0.25 -0.6 1,664,028 + Pf1N1B4_1450 0.25 -0.8 1,664,028 + Pf1N1B4_1450 0.25 +2.2 1,664,029 - Pf1N1B4_1450 0.25 -1.3 1,664,029 - Pf1N1B4_1450 0.25 -0.1 1,664,029 - Pf1N1B4_1450 0.25 -0.3 1,664,029 - Pf1N1B4_1450 0.25 +0.4 1,664,079 + Pf1N1B4_1450 0.30 -0.3 1,664,183 - Pf1N1B4_1450 0.42 -1.2 1,664,241 + Pf1N1B4_1450 0.49 +2.2 1,664,241 + Pf1N1B4_1450 0.49 +1.6 1,664,536 + Pf1N1B4_1450 0.82 -0.6 1,664,536 + Pf1N1B4_1450 0.82 -0.0 1,664,536 + Pf1N1B4_1450 0.82 -1.9 1,664,537 - Pf1N1B4_1450 0.82 -0.8 1,664,724 - +1.2 1,664,756 + +1.6 1,664,756 + +0.2 1,664,756 + -0.3 1,664,757 - -0.1 1,664,757 - +0.2 1,664,757 - -0.8 1,664,757 - -0.3 1,664,788 - -2.3 1,664,811 + -0.7 1,664,811 + +0.7 1,664,811 + -1.6 1,664,812 - -1.5 1,664,812 - -0.3 1,664,812 - -0.8 1,664,812 - -1.1 1,664,812 - -0.8 1,664,812 - -1.6 1,664,814 + -2.1 1,664,814 + -2.0 1,664,814 + +0.7 1,664,814 + -1.6 1,664,814 + -0.3 1,664,814 + -2.8 1,664,814 + -1.9 1,664,814 + -1.1 1,664,814 + -1.1 1,664,814 + -0.9 1,664,814 + -1.3 1,664,814 + -0.3 1,664,814 + -2.1 1,664,814 + +0.7 1,664,814 + -1.1 1,664,815 - -0.3 1,664,815 - -0.1 1,664,815 - +1.2 1,664,815 - -0.9 1,664,815 - -0.8 1,664,815 - -1.6 1,664,815 - -0.3 1,664,815 - -1.8 1,664,815 - -0.1 1,664,815 - -2.1 1,664,815 - -2.5 1,664,815 - -0.6 1,664,815 - -1.8 1,664,817 + -2.1 1,664,817 + +0.2 1,664,817 + -2.6 1,664,817 + +0.2 1,664,817 + +0.5 1,664,817 + -0.3 1,664,817 + -0.3 1,664,817 + -0.3 1,664,817 + -0.3 1,664,818 - -2.6 1,664,818 - +0.2 1,664,818 - -1.6 1,664,818 - -1.1 1,664,818 - +0.7 1,664,818 - -0.8 1,664,879 + Pf1N1B4_1451 0.23 -0.8 1,664,880 - Pf1N1B4_1451 0.23 -0.3 1,665,033 - Pf1N1B4_1451 0.69 -1.2 1,665,033 - Pf1N1B4_1451 0.69 +0.7 1,665,049 + Pf1N1B4_1451 0.74 -1.6 1,665,049 + Pf1N1B4_1451 0.74 -2.1 1,665,049 + Pf1N1B4_1451 0.74 +1.5 1,665,049 + Pf1N1B4_1451 0.74 +0.2 1,665,049 + Pf1N1B4_1451 0.74 -2.2 1,665,049 + Pf1N1B4_1451 0.74 -0.1 1,665,050 - Pf1N1B4_1451 0.74 +0.2 1,665,050 - Pf1N1B4_1451 0.74 -1.3 1,665,085 + Pf1N1B4_1451 0.85 -1.1 1,665,097 + Pf1N1B4_1451 0.89 -1.5 1,665,097 + Pf1N1B4_1451 0.89 -0.8 1,665,098 - Pf1N1B4_1451 0.89 +0.2 1,665,132 + -0.9 1,665,132 + +0.7 1,665,296 + -1.1 1,665,296 + +0.8 1,665,296 + +3.0 1,665,296 + -0.1 1,665,297 - -2.2 1,665,297 - -1.9 1,665,298 + +2.2
Or see this region's nucleotide sequence