Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1143

Experiment: Choline chloride 200 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1142 and Pf1N1B4_1143 are separated by 172 nucleotidesPf1N1B4_1143 and Pf1N1B4_1144 are separated by 167 nucleotides Pf1N1B4_1142 - Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB, at 1,282,371 to 1,284,899 _1142 Pf1N1B4_1143 - Phosphodiesterase/alkaline phosphatase D, at 1,285,072 to 1,286,613 _1143 Pf1N1B4_1144 - PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69), at 1,286,781 to 1,288,520 _1144 Position (kb) 1285 1286 1287Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1284.313 kb on - strand, within Pf1N1B4_1142at 1284.313 kb on - strand, within Pf1N1B4_1142at 1284.313 kb on - strand, within Pf1N1B4_1142at 1284.616 kb on - strand, within Pf1N1B4_1142at 1284.616 kb on - strand, within Pf1N1B4_1142at 1284.708 kb on + strandat 1284.708 kb on + strandat 1284.708 kb on + strandat 1284.708 kb on + strandat 1284.708 kb on + strandat 1284.708 kb on + strandat 1284.708 kb on + strandat 1284.708 kb on + strandat 1284.709 kb on - strandat 1284.709 kb on - strandat 1284.709 kb on - strandat 1284.777 kb on + strandat 1284.778 kb on - strandat 1284.814 kb on + strandat 1284.814 kb on + strandat 1284.815 kb on - strandat 1284.910 kb on + strandat 1284.910 kb on + strandat 1284.911 kb on - strandat 1284.945 kb on + strandat 1284.945 kb on + strandat 1284.946 kb on - strandat 1285.398 kb on + strand, within Pf1N1B4_1143at 1285.398 kb on + strand, within Pf1N1B4_1143at 1285.399 kb on - strand, within Pf1N1B4_1143at 1285.399 kb on - strand, within Pf1N1B4_1143at 1285.444 kb on + strand, within Pf1N1B4_1143at 1285.712 kb on + strand, within Pf1N1B4_1143at 1285.713 kb on - strand, within Pf1N1B4_1143at 1285.772 kb on + strand, within Pf1N1B4_1143at 1285.815 kb on + strand, within Pf1N1B4_1143at 1285.829 kb on + strand, within Pf1N1B4_1143at 1285.946 kb on + strand, within Pf1N1B4_1143at 1285.953 kb on - strand, within Pf1N1B4_1143at 1285.965 kb on - strand, within Pf1N1B4_1143at 1286.070 kb on - strand, within Pf1N1B4_1143at 1286.126 kb on + strand, within Pf1N1B4_1143at 1286.126 kb on + strand, within Pf1N1B4_1143at 1286.126 kb on + strand, within Pf1N1B4_1143at 1286.127 kb on - strand, within Pf1N1B4_1143at 1286.127 kb on - strand, within Pf1N1B4_1143at 1286.203 kb on - strand, within Pf1N1B4_1143at 1286.388 kb on + strandat 1286.640 kb on + strandat 1286.640 kb on + strandat 1286.641 kb on - strandat 1286.641 kb on - strandat 1286.641 kb on - strandat 1286.641 kb on - strandat 1286.641 kb on - strandat 1286.738 kb on + strandat 1286.776 kb on + strandat 1286.777 kb on - strandat 1286.839 kb on + strandat 1286.839 kb on + strandat 1286.840 kb on - strandat 1286.840 kb on - strandat 1286.840 kb on - strandat 1286.840 kb on - strandat 1286.884 kb on + strandat 1286.884 kb on + strandat 1286.884 kb on + strandat 1286.885 kb on - strandat 1286.965 kb on + strand, within Pf1N1B4_1144at 1286.965 kb on + strand, within Pf1N1B4_1144at 1286.966 kb on - strand, within Pf1N1B4_1144at 1286.966 kb on - strand, within Pf1N1B4_1144at 1286.966 kb on - strand, within Pf1N1B4_1144at 1286.966 kb on - strand, within Pf1N1B4_1144at 1286.966 kb on - strand, within Pf1N1B4_1144at 1286.966 kb on - strand, within Pf1N1B4_1144at 1287.063 kb on - strand, within Pf1N1B4_1144at 1287.063 kb on - strand, within Pf1N1B4_1144at 1287.063 kb on - strand, within Pf1N1B4_1144at 1287.203 kb on - strand, within Pf1N1B4_1144at 1287.385 kb on + strand, within Pf1N1B4_1144at 1287.385 kb on + strand, within Pf1N1B4_1144at 1287.385 kb on + strand, within Pf1N1B4_1144at 1287.385 kb on + strand, within Pf1N1B4_1144at 1287.385 kb on + strand, within Pf1N1B4_1144at 1287.385 kb on + strand, within Pf1N1B4_1144at 1287.385 kb on + strand, within Pf1N1B4_1144at 1287.385 kb on + strand, within Pf1N1B4_1144at 1287.385 kb on + strand, within Pf1N1B4_1144at 1287.385 kb on + strand, within Pf1N1B4_1144at 1287.385 kb on + strandat 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.386 kb on - strand, within Pf1N1B4_1144at 1287.475 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Choline chloride 200 mM
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1,284,313 - Pf1N1B4_1142 0.77 -2.2
1,284,313 - Pf1N1B4_1142 0.77 +0.4
1,284,313 - Pf1N1B4_1142 0.77 +0.5
1,284,616 - Pf1N1B4_1142 0.89 +3.6
1,284,616 - Pf1N1B4_1142 0.89 +2.0
1,284,708 + +1.7
1,284,708 + +3.6
1,284,708 + +1.5
1,284,708 + +0.4
1,284,708 + +0.2
1,284,708 + +2.8
1,284,708 + +1.7
1,284,708 + +0.0
1,284,709 - +2.2
1,284,709 - +0.8
1,284,709 - -0.1
1,284,777 + +0.8
1,284,778 - +1.1
1,284,814 + -1.1
1,284,814 + -0.8
1,284,815 - +0.2
1,284,910 + +0.2
1,284,910 + +0.8
1,284,911 - -0.5
1,284,945 + -1.6
1,284,945 + +1.5
1,284,946 - +0.8
1,285,398 + Pf1N1B4_1143 0.21 +1.5
1,285,398 + Pf1N1B4_1143 0.21 -1.3
1,285,399 - Pf1N1B4_1143 0.21 +0.0
1,285,399 - Pf1N1B4_1143 0.21 -1.2
1,285,444 + Pf1N1B4_1143 0.24 +0.2
1,285,712 + Pf1N1B4_1143 0.42 -0.8
1,285,713 - Pf1N1B4_1143 0.42 -0.8
1,285,772 + Pf1N1B4_1143 0.45 -0.3
1,285,815 + Pf1N1B4_1143 0.48 -0.9
1,285,829 + Pf1N1B4_1143 0.49 +0.7
1,285,946 + Pf1N1B4_1143 0.57 +0.5
1,285,953 - Pf1N1B4_1143 0.57 -1.2
1,285,965 - Pf1N1B4_1143 0.58 -1.6
1,286,070 - Pf1N1B4_1143 0.65 -1.3
1,286,126 + Pf1N1B4_1143 0.68 -2.9
1,286,126 + Pf1N1B4_1143 0.68 -2.0
1,286,126 + Pf1N1B4_1143 0.68 -0.1
1,286,127 - Pf1N1B4_1143 0.68 +0.7
1,286,127 - Pf1N1B4_1143 0.68 -1.2
1,286,203 - Pf1N1B4_1143 0.73 -1.3
1,286,388 + -1.6
1,286,640 + -0.1
1,286,640 + -0.5
1,286,641 - -0.8
1,286,641 - -1.8
1,286,641 - -1.6
1,286,641 - +0.8
1,286,641 - +1.7
1,286,738 + -1.6
1,286,776 + +2.2
1,286,777 - -1.1
1,286,839 + -0.8
1,286,839 + +0.5
1,286,840 - +0.1
1,286,840 - -2.1
1,286,840 - +0.2
1,286,840 - +0.5
1,286,884 + -1.4
1,286,884 + -1.8
1,286,884 + +0.2
1,286,885 - -1.8
1,286,965 + Pf1N1B4_1144 0.11 -0.4
1,286,965 + Pf1N1B4_1144 0.11 +0.8
1,286,966 - Pf1N1B4_1144 0.11 -0.8
1,286,966 - Pf1N1B4_1144 0.11 -1.6
1,286,966 - Pf1N1B4_1144 0.11 -1.4
1,286,966 - Pf1N1B4_1144 0.11 -1.1
1,286,966 - Pf1N1B4_1144 0.11 +0.9
1,286,966 - Pf1N1B4_1144 0.11 -1.1
1,287,063 - Pf1N1B4_1144 0.16 -2.6
1,287,063 - Pf1N1B4_1144 0.16 -1.1
1,287,063 - Pf1N1B4_1144 0.16 +1.6
1,287,203 - Pf1N1B4_1144 0.24 -1.6
1,287,385 + Pf1N1B4_1144 0.35 -1.5
1,287,385 + Pf1N1B4_1144 0.35 +0.2
1,287,385 + Pf1N1B4_1144 0.35 -0.5
1,287,385 + Pf1N1B4_1144 0.35 -0.4
1,287,385 + Pf1N1B4_1144 0.35 +0.1
1,287,385 + Pf1N1B4_1144 0.35 -1.8
1,287,385 + Pf1N1B4_1144 0.35 -1.5
1,287,385 + Pf1N1B4_1144 0.35 -0.8
1,287,385 + Pf1N1B4_1144 0.35 +0.7
1,287,385 + Pf1N1B4_1144 0.35 -0.6
1,287,385 + -0.8
1,287,386 - Pf1N1B4_1144 0.35 -0.6
1,287,386 - Pf1N1B4_1144 0.35 +1.5
1,287,386 - Pf1N1B4_1144 0.35 -0.9
1,287,386 - Pf1N1B4_1144 0.35 -1.9
1,287,386 - Pf1N1B4_1144 0.35 +0.6
1,287,386 - Pf1N1B4_1144 0.35 -1.1
1,287,386 - Pf1N1B4_1144 0.35 -1.1
1,287,386 - Pf1N1B4_1144 0.35 -1.1
1,287,386 - Pf1N1B4_1144 0.35 -0.1
1,287,386 - Pf1N1B4_1144 0.35 -0.4
1,287,386 - Pf1N1B4_1144 0.35 +0.0
1,287,386 - Pf1N1B4_1144 0.35 +0.0
1,287,386 - Pf1N1B4_1144 0.35 +0.4
1,287,386 - Pf1N1B4_1144 0.35 +1.1
1,287,386 - Pf1N1B4_1144 0.35 +0.1
1,287,386 - Pf1N1B4_1144 0.35 +1.1
1,287,386 - Pf1N1B4_1144 0.35 -0.8
1,287,386 - Pf1N1B4_1144 0.35 -1.8
1,287,386 - Pf1N1B4_1144 0.35 -0.3
1,287,475 + +1.2

Or see this region's nucleotide sequence