Experiment: no_plant_rhizosphere sample 2; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS18710 and ABZR87_RS18715 are separated by 198 nucleotides ABZR87_RS18715 and ABZR87_RS18720 are separated by 82 nucleotides ABZR87_RS18720 and ABZR87_RS18725 overlap by 1 nucleotides
ABZR87_RS18710: ABZR87_RS18710 - hypothetical protein, at 549,611 to 550,792
_RS18710
ABZR87_RS18715: ABZR87_RS18715 - DUF2946 domain-containing protein, at 550,991 to 551,392
_RS18715
ABZR87_RS18720: ABZR87_RS18720 - TonB-dependent receptor, at 551,475 to 553,793
_RS18720
ABZR87_RS18725: ABZR87_RS18725 - sialidase family protein, at 553,793 to 555,046
_RS18725
Position (kb) 551
552
553
554 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 550.580 kb on + strand, within ABZR87_RS18710 at 550.580 kb on + strand, within ABZR87_RS18710 at 550.580 kb on + strand, within ABZR87_RS18710 at 550.580 kb on + strand, within ABZR87_RS18710 at 550.580 kb on + strand, within ABZR87_RS18710 at 550.581 kb on - strand, within ABZR87_RS18710 at 550.581 kb on - strand, within ABZR87_RS18710 at 550.581 kb on - strand, within ABZR87_RS18710 at 550.590 kb on + strand, within ABZR87_RS18710 at 550.590 kb on + strand, within ABZR87_RS18710 at 550.590 kb on + strand, within ABZR87_RS18710 at 550.590 kb on + strand, within ABZR87_RS18710 at 550.590 kb on + strand, within ABZR87_RS18710 at 550.590 kb on + strand, within ABZR87_RS18710 at 550.590 kb on + strand, within ABZR87_RS18710 at 550.591 kb on - strand, within ABZR87_RS18710 at 550.591 kb on - strand, within ABZR87_RS18710 at 550.591 kb on - strand, within ABZR87_RS18710 at 550.591 kb on - strand, within ABZR87_RS18710 at 550.591 kb on - strand, within ABZR87_RS18710 at 550.591 kb on - strand, within ABZR87_RS18710 at 550.591 kb on - strand, within ABZR87_RS18710 at 550.591 kb on - strand, within ABZR87_RS18710 at 550.591 kb on - strand, within ABZR87_RS18710 at 550.591 kb on - strand, within ABZR87_RS18710 at 550.861 kb on - strand at 550.861 kb on - strand at 550.861 kb on - strand at 550.961 kb on - strand at 550.962 kb on + strand at 550.962 kb on + strand at 550.962 kb on + strand at 550.962 kb on + strand at 550.963 kb on - strand at 550.986 kb on + strand at 550.987 kb on - strand at 550.987 kb on - strand at 550.987 kb on - strand at 550.987 kb on - strand at 550.987 kb on - strand at 550.991 kb on - strand at 551.034 kb on + strand, within ABZR87_RS18715 at 551.035 kb on - strand, within ABZR87_RS18715 at 551.035 kb on - strand, within ABZR87_RS18715 at 551.035 kb on - strand, within ABZR87_RS18715 at 551.043 kb on + strand, within ABZR87_RS18715 at 551.043 kb on + strand, within ABZR87_RS18715 at 551.043 kb on + strand, within ABZR87_RS18715 at 551.044 kb on - strand, within ABZR87_RS18715 at 551.044 kb on - strand, within ABZR87_RS18715 at 551.044 kb on - strand, within ABZR87_RS18715 at 551.044 kb on - strand, within ABZR87_RS18715 at 551.045 kb on - strand, within ABZR87_RS18715 at 551.213 kb on + strand, within ABZR87_RS18715 at 551.213 kb on + strand, within ABZR87_RS18715 at 551.213 kb on + strand, within ABZR87_RS18715 at 551.214 kb on - strand, within ABZR87_RS18715 at 551.214 kb on - strand, within ABZR87_RS18715 at 551.288 kb on + strand, within ABZR87_RS18715 at 551.288 kb on + strand, within ABZR87_RS18715 at 551.288 kb on + strand, within ABZR87_RS18715 at 551.289 kb on - strand, within ABZR87_RS18715 at 551.289 kb on - strand, within ABZR87_RS18715 at 551.324 kb on + strand, within ABZR87_RS18715 at 551.325 kb on - strand, within ABZR87_RS18715 at 551.407 kb on + strand at 551.407 kb on + strand at 551.407 kb on + strand at 551.407 kb on + strand at 551.407 kb on + strand at 551.408 kb on - strand at 551.673 kb on - strand at 551.673 kb on - strand at 551.673 kb on - strand at 551.752 kb on - strand, within ABZR87_RS18720 at 551.752 kb on - strand, within ABZR87_RS18720 at 551.752 kb on - strand, within ABZR87_RS18720 at 551.853 kb on + strand, within ABZR87_RS18720 at 551.853 kb on + strand, within ABZR87_RS18720 at 551.853 kb on + strand, within ABZR87_RS18720 at 551.854 kb on - strand, within ABZR87_RS18720 at 551.854 kb on - strand, within ABZR87_RS18720 at 551.854 kb on - strand, within ABZR87_RS18720 at 551.854 kb on - strand, within ABZR87_RS18720 at 551.854 kb on - strand, within ABZR87_RS18720 at 551.895 kb on + strand, within ABZR87_RS18720 at 551.895 kb on + strand, within ABZR87_RS18720 at 551.896 kb on - strand, within ABZR87_RS18720 at 551.951 kb on + strand, within ABZR87_RS18720 at 551.952 kb on - strand, within ABZR87_RS18720 at 551.954 kb on + strand, within ABZR87_RS18720 at 551.958 kb on + strand, within ABZR87_RS18720 at 551.958 kb on + strand, within ABZR87_RS18720 at 551.958 kb on + strand, within ABZR87_RS18720 at 551.959 kb on - strand, within ABZR87_RS18720 at 552.075 kb on + strand, within ABZR87_RS18720 at 552.075 kb on + strand, within ABZR87_RS18720 at 552.076 kb on - strand, within ABZR87_RS18720 at 552.076 kb on - strand, within ABZR87_RS18720 at 552.087 kb on + strand, within ABZR87_RS18720 at 552.088 kb on - strand, within ABZR87_RS18720 at 552.159 kb on + strand, within ABZR87_RS18720 at 552.160 kb on - strand, within ABZR87_RS18720 at 552.160 kb on - strand, within ABZR87_RS18720 at 552.207 kb on + strand, within ABZR87_RS18720 at 552.207 kb on + strand, within ABZR87_RS18720 at 552.208 kb on - strand, within ABZR87_RS18720 at 552.208 kb on - strand, within ABZR87_RS18720 at 552.364 kb on - strand, within ABZR87_RS18720 at 552.364 kb on - strand, within ABZR87_RS18720 at 552.504 kb on + strand, within ABZR87_RS18720 at 552.505 kb on - strand, within ABZR87_RS18720 at 552.505 kb on - strand, within ABZR87_RS18720 at 552.505 kb on - strand, within ABZR87_RS18720 at 552.562 kb on - strand, within ABZR87_RS18720 at 552.602 kb on - strand, within ABZR87_RS18720 at 552.621 kb on + strand, within ABZR87_RS18720 at 552.621 kb on + strand, within ABZR87_RS18720 at 552.621 kb on + strand, within ABZR87_RS18720 at 552.690 kb on + strand, within ABZR87_RS18720 at 552.755 kb on + strand, within ABZR87_RS18720 at 552.755 kb on + strand, within ABZR87_RS18720 at 552.755 kb on + strand, within ABZR87_RS18720 at 552.755 kb on + strand, within ABZR87_RS18720 at 552.756 kb on - strand, within ABZR87_RS18720 at 552.756 kb on - strand, within ABZR87_RS18720 at 552.756 kb on - strand, within ABZR87_RS18720 at 552.756 kb on - strand, within ABZR87_RS18720 at 552.756 kb on - strand, within ABZR87_RS18720 at 552.778 kb on - strand, within ABZR87_RS18720 at 552.778 kb on - strand, within ABZR87_RS18720 at 552.786 kb on + strand, within ABZR87_RS18720 at 552.787 kb on - strand, within ABZR87_RS18720 at 552.787 kb on - strand, within ABZR87_RS18720 at 552.787 kb on - strand, within ABZR87_RS18720 at 552.787 kb on - strand, within ABZR87_RS18720 at 552.839 kb on - strand, within ABZR87_RS18720 at 552.963 kb on + strand, within ABZR87_RS18720 at 552.964 kb on - strand, within ABZR87_RS18720 at 553.241 kb on + strand, within ABZR87_RS18720 at 553.242 kb on - strand, within ABZR87_RS18720 at 553.242 kb on - strand, within ABZR87_RS18720 at 553.278 kb on + strand, within ABZR87_RS18720 at 553.278 kb on + strand, within ABZR87_RS18720 at 553.279 kb on - strand, within ABZR87_RS18720 at 553.279 kb on - strand, within ABZR87_RS18720 at 553.279 kb on - strand, within ABZR87_RS18720 at 553.365 kb on + strand, within ABZR87_RS18720 at 553.473 kb on + strand, within ABZR87_RS18720 at 553.473 kb on + strand, within ABZR87_RS18720 at 553.474 kb on - strand, within ABZR87_RS18720 at 553.626 kb on + strand at 553.627 kb on - strand at 553.671 kb on + strand at 553.676 kb on + strand at 553.732 kb on - strand at 553.740 kb on + strand at 553.741 kb on - strand at 553.767 kb on + strand at 553.767 kb on + strand at 553.768 kb on - strand at 553.768 kb on - strand at 553.768 kb on - strand at 553.768 kb on - strand at 553.768 kb on - strand at 553.768 kb on - strand at 553.768 kb on - strand at 553.791 kb on + strand at 553.791 kb on + strand at 553.791 kb on + strand at 553.792 kb on - strand at 553.792 kb on - strand at 553.792 kb on - strand at 554.057 kb on + strand, within ABZR87_RS18725 at 554.057 kb on + strand, within ABZR87_RS18725 at 554.057 kb on + strand, within ABZR87_RS18725 at 554.057 kb on + strand, within ABZR87_RS18725 at 554.057 kb on + strand, within ABZR87_RS18725 at 554.057 kb on + strand, within ABZR87_RS18725 at 554.058 kb on - strand, within ABZR87_RS18725 at 554.058 kb on - strand, within ABZR87_RS18725 at 554.058 kb on - strand, within ABZR87_RS18725 at 554.111 kb on + strand, within ABZR87_RS18725 at 554.111 kb on + strand, within ABZR87_RS18725 at 554.111 kb on + strand, within ABZR87_RS18725 at 554.111 kb on + strand, within ABZR87_RS18725 at 554.111 kb on + strand, within ABZR87_RS18725 at 554.111 kb on + strand, within ABZR87_RS18725 at 554.111 kb on + strand, within ABZR87_RS18725 at 554.111 kb on + strand, within ABZR87_RS18725 at 554.111 kb on + strand, within ABZR87_RS18725 at 554.111 kb on + strand, within ABZR87_RS18725 at 554.112 kb on - strand, within ABZR87_RS18725 at 554.112 kb on - strand, within ABZR87_RS18725 at 554.112 kb on - strand, within ABZR87_RS18725 at 554.112 kb on - strand, within ABZR87_RS18725 at 554.112 kb on - strand, within ABZR87_RS18725 at 554.112 kb on - strand, within ABZR87_RS18725 at 554.144 kb on + strand, within ABZR87_RS18725 at 554.144 kb on + strand, within ABZR87_RS18725 at 554.144 kb on + strand, within ABZR87_RS18725 at 554.337 kb on - strand, within ABZR87_RS18725 at 554.354 kb on + strand, within ABZR87_RS18725 at 554.354 kb on + strand, within ABZR87_RS18725 at 554.354 kb on + strand, within ABZR87_RS18725 at 554.355 kb on - strand, within ABZR87_RS18725 at 554.357 kb on + strand, within ABZR87_RS18725 at 554.357 kb on + strand, within ABZR87_RS18725 at 554.357 kb on + strand, within ABZR87_RS18725 at 554.357 kb on + strand, within ABZR87_RS18725 at 554.357 kb on + strand, within ABZR87_RS18725 at 554.357 kb on + strand, within ABZR87_RS18725 at 554.358 kb on - strand, within ABZR87_RS18725 at 554.358 kb on - strand, within ABZR87_RS18725 at 554.358 kb on - strand, within ABZR87_RS18725 at 554.358 kb on - strand, within ABZR87_RS18725 at 554.358 kb on - strand, within ABZR87_RS18725 at 554.536 kb on + strand, within ABZR87_RS18725 at 554.537 kb on - strand, within ABZR87_RS18725
Per-strain Table
Position Strand Gene LocusTag Fraction no_plant_rhizosphere sample 2; outgrowth in LB remove 550,580 + ABZR87_RS18710 0.82 -1.5 550,580 + ABZR87_RS18710 0.82 -0.7 550,580 + ABZR87_RS18710 0.82 +1.3 550,580 + ABZR87_RS18710 0.82 -0.1 550,580 + ABZR87_RS18710 0.82 +0.3 550,581 - ABZR87_RS18710 0.82 -0.6 550,581 - ABZR87_RS18710 0.82 +1.2 550,581 - ABZR87_RS18710 0.82 -0.9 550,590 + ABZR87_RS18710 0.83 +0.1 550,590 + ABZR87_RS18710 0.83 -0.6 550,590 + ABZR87_RS18710 0.83 -1.0 550,590 + ABZR87_RS18710 0.83 -0.2 550,590 + ABZR87_RS18710 0.83 -1.4 550,590 + ABZR87_RS18710 0.83 +0.8 550,590 + ABZR87_RS18710 0.83 +1.5 550,591 - ABZR87_RS18710 0.83 +0.3 550,591 - ABZR87_RS18710 0.83 +0.3 550,591 - ABZR87_RS18710 0.83 -3.1 550,591 - ABZR87_RS18710 0.83 -0.8 550,591 - ABZR87_RS18710 0.83 -0.5 550,591 - ABZR87_RS18710 0.83 +0.2 550,591 - ABZR87_RS18710 0.83 +0.8 550,591 - ABZR87_RS18710 0.83 +1.1 550,591 - ABZR87_RS18710 0.83 +0.8 550,591 - ABZR87_RS18710 0.83 -1.6 550,861 - +2.4 550,861 - -0.2 550,861 - +1.4 550,961 - -0.4 550,962 + -2.2 550,962 + -1.5 550,962 + -1.1 550,962 + +0.4 550,963 - +0.7 550,986 + +0.0 550,987 - +0.5 550,987 - +0.6 550,987 - +0.1 550,987 - -0.9 550,987 - -0.2 550,991 - -0.2 551,034 + ABZR87_RS18715 0.11 +1.3 551,035 - ABZR87_RS18715 0.11 -1.1 551,035 - ABZR87_RS18715 0.11 -1.0 551,035 - ABZR87_RS18715 0.11 -1.0 551,043 + ABZR87_RS18715 0.13 +0.7 551,043 + ABZR87_RS18715 0.13 +1.5 551,043 + ABZR87_RS18715 0.13 +1.0 551,044 - ABZR87_RS18715 0.13 -0.2 551,044 - ABZR87_RS18715 0.13 +0.5 551,044 - ABZR87_RS18715 0.13 +1.1 551,044 - ABZR87_RS18715 0.13 +1.2 551,045 - ABZR87_RS18715 0.13 +0.3 551,213 + ABZR87_RS18715 0.55 -1.4 551,213 + ABZR87_RS18715 0.55 +0.4 551,213 + ABZR87_RS18715 0.55 -1.6 551,214 - ABZR87_RS18715 0.55 -1.3 551,214 - ABZR87_RS18715 0.55 +0.0 551,288 + ABZR87_RS18715 0.74 +0.5 551,288 + ABZR87_RS18715 0.74 +1.1 551,288 + ABZR87_RS18715 0.74 +0.6 551,289 - ABZR87_RS18715 0.74 +0.1 551,289 - ABZR87_RS18715 0.74 -0.2 551,324 + ABZR87_RS18715 0.83 -0.6 551,325 - ABZR87_RS18715 0.83 -0.5 551,407 + -1.3 551,407 + +1.2 551,407 + -1.2 551,407 + -0.6 551,407 + -0.1 551,408 - -1.1 551,673 - +0.3 551,673 - +0.5 551,673 - -0.4 551,752 - ABZR87_RS18720 0.12 -0.1 551,752 - ABZR87_RS18720 0.12 -0.8 551,752 - ABZR87_RS18720 0.12 -0.2 551,853 + ABZR87_RS18720 0.16 -0.7 551,853 + ABZR87_RS18720 0.16 +0.6 551,853 + ABZR87_RS18720 0.16 +0.1 551,854 - ABZR87_RS18720 0.16 -0.4 551,854 - ABZR87_RS18720 0.16 -0.0 551,854 - ABZR87_RS18720 0.16 -0.2 551,854 - ABZR87_RS18720 0.16 -0.4 551,854 - ABZR87_RS18720 0.16 +0.7 551,895 + ABZR87_RS18720 0.18 -1.8 551,895 + ABZR87_RS18720 0.18 -0.2 551,896 - ABZR87_RS18720 0.18 +0.2 551,951 + ABZR87_RS18720 0.21 -0.2 551,952 - ABZR87_RS18720 0.21 +2.3 551,954 + ABZR87_RS18720 0.21 -0.4 551,958 + ABZR87_RS18720 0.21 -1.0 551,958 + ABZR87_RS18720 0.21 +0.9 551,958 + ABZR87_RS18720 0.21 -0.8 551,959 - ABZR87_RS18720 0.21 +0.7 552,075 + ABZR87_RS18720 0.26 -0.4 552,075 + ABZR87_RS18720 0.26 -1.2 552,076 - ABZR87_RS18720 0.26 -1.9 552,076 - ABZR87_RS18720 0.26 -1.6 552,087 + ABZR87_RS18720 0.26 -0.4 552,088 - ABZR87_RS18720 0.26 +0.2 552,159 + ABZR87_RS18720 0.29 -1.3 552,160 - ABZR87_RS18720 0.30 -0.9 552,160 - ABZR87_RS18720 0.30 +0.8 552,207 + ABZR87_RS18720 0.32 +0.4 552,207 + ABZR87_RS18720 0.32 -0.5 552,208 - ABZR87_RS18720 0.32 +0.7 552,208 - ABZR87_RS18720 0.32 +1.0 552,364 - ABZR87_RS18720 0.38 -0.8 552,364 - ABZR87_RS18720 0.38 -1.3 552,504 + ABZR87_RS18720 0.44 -1.9 552,505 - ABZR87_RS18720 0.44 -1.7 552,505 - ABZR87_RS18720 0.44 -1.7 552,505 - ABZR87_RS18720 0.44 -0.1 552,562 - ABZR87_RS18720 0.47 +1.1 552,602 - ABZR87_RS18720 0.49 -1.0 552,621 + ABZR87_RS18720 0.49 -0.5 552,621 + ABZR87_RS18720 0.49 -0.8 552,621 + ABZR87_RS18720 0.49 -0.3 552,690 + ABZR87_RS18720 0.52 -0.3 552,755 + ABZR87_RS18720 0.55 +0.8 552,755 + ABZR87_RS18720 0.55 +0.2 552,755 + ABZR87_RS18720 0.55 +0.2 552,755 + ABZR87_RS18720 0.55 -0.0 552,756 - ABZR87_RS18720 0.55 +0.7 552,756 - ABZR87_RS18720 0.55 -1.5 552,756 - ABZR87_RS18720 0.55 -1.7 552,756 - ABZR87_RS18720 0.55 +0.1 552,756 - ABZR87_RS18720 0.55 -0.3 552,778 - ABZR87_RS18720 0.56 -0.2 552,778 - ABZR87_RS18720 0.56 +0.1 552,786 + ABZR87_RS18720 0.57 +0.1 552,787 - ABZR87_RS18720 0.57 +0.4 552,787 - ABZR87_RS18720 0.57 -0.6 552,787 - ABZR87_RS18720 0.57 -2.8 552,787 - ABZR87_RS18720 0.57 -2.0 552,839 - ABZR87_RS18720 0.59 -0.9 552,963 + ABZR87_RS18720 0.64 +0.5 552,964 - ABZR87_RS18720 0.64 +0.4 553,241 + ABZR87_RS18720 0.76 -1.1 553,242 - ABZR87_RS18720 0.76 -0.9 553,242 - ABZR87_RS18720 0.76 -2.2 553,278 + ABZR87_RS18720 0.78 -0.1 553,278 + ABZR87_RS18720 0.78 -2.3 553,279 - ABZR87_RS18720 0.78 -0.7 553,279 - ABZR87_RS18720 0.78 +0.1 553,279 - ABZR87_RS18720 0.78 +0.5 553,365 + ABZR87_RS18720 0.82 +0.2 553,473 + ABZR87_RS18720 0.86 -0.2 553,473 + ABZR87_RS18720 0.86 -0.4 553,474 - ABZR87_RS18720 0.86 +3.6 553,626 + -0.4 553,627 - +1.2 553,671 + -0.3 553,676 + +2.0 553,732 - +0.0 553,740 + -0.5 553,741 - +0.8 553,767 + +2.9 553,767 + -1.5 553,768 - -0.2 553,768 - -0.5 553,768 - -1.1 553,768 - -2.0 553,768 - -1.4 553,768 - -2.0 553,768 - +0.1 553,791 + +2.9 553,791 + +0.5 553,791 + +0.0 553,792 - +0.1 553,792 - -0.1 553,792 - +0.0 554,057 + ABZR87_RS18725 0.21 -1.0 554,057 + ABZR87_RS18725 0.21 -0.2 554,057 + ABZR87_RS18725 0.21 -1.9 554,057 + ABZR87_RS18725 0.21 +0.6 554,057 + ABZR87_RS18725 0.21 +2.3 554,057 + ABZR87_RS18725 0.21 -0.2 554,058 - ABZR87_RS18725 0.21 +1.0 554,058 - ABZR87_RS18725 0.21 +0.1 554,058 - ABZR87_RS18725 0.21 -1.0 554,111 + ABZR87_RS18725 0.25 +1.6 554,111 + ABZR87_RS18725 0.25 -0.5 554,111 + ABZR87_RS18725 0.25 +0.2 554,111 + ABZR87_RS18725 0.25 -1.1 554,111 + ABZR87_RS18725 0.25 +0.9 554,111 + ABZR87_RS18725 0.25 -0.1 554,111 + ABZR87_RS18725 0.25 +0.2 554,111 + ABZR87_RS18725 0.25 -1.4 554,111 + ABZR87_RS18725 0.25 -0.5 554,111 + ABZR87_RS18725 0.25 +1.3 554,112 - ABZR87_RS18725 0.25 +0.9 554,112 - ABZR87_RS18725 0.25 +0.4 554,112 - ABZR87_RS18725 0.25 -1.3 554,112 - ABZR87_RS18725 0.25 -1.3 554,112 - ABZR87_RS18725 0.25 -1.0 554,112 - ABZR87_RS18725 0.25 -1.2 554,144 + ABZR87_RS18725 0.28 -0.5 554,144 + ABZR87_RS18725 0.28 -0.2 554,144 + ABZR87_RS18725 0.28 +0.6 554,337 - ABZR87_RS18725 0.43 +2.3 554,354 + ABZR87_RS18725 0.45 -0.3 554,354 + ABZR87_RS18725 0.45 -0.4 554,354 + ABZR87_RS18725 0.45 +0.5 554,355 - ABZR87_RS18725 0.45 +0.5 554,357 + ABZR87_RS18725 0.45 +0.2 554,357 + ABZR87_RS18725 0.45 -0.0 554,357 + ABZR87_RS18725 0.45 -0.0 554,357 + ABZR87_RS18725 0.45 +0.3 554,357 + ABZR87_RS18725 0.45 +2.3 554,357 + ABZR87_RS18725 0.45 -1.8 554,358 - ABZR87_RS18725 0.45 +0.5 554,358 - ABZR87_RS18725 0.45 +0.2 554,358 - ABZR87_RS18725 0.45 +1.1 554,358 - ABZR87_RS18725 0.45 +0.5 554,358 - ABZR87_RS18725 0.45 -1.2 554,536 + ABZR87_RS18725 0.59 +0.4 554,537 - ABZR87_RS18725 0.59 +0.2
Or see this region's nucleotide sequence