Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_72

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_71 and Pf1N1B4_72 are separated by 123 nucleotidesPf1N1B4_72 and Pf1N1B4_73 are separated by 142 nucleotides Pf1N1B4_71 - Glucarate dehydratase (EC 4.2.1.40), at 74,280 to 75,623 _71 Pf1N1B4_72 - Transcriptional regulator, LysR family, at 75,747 to 76,619 _72 Pf1N1B4_73 - Beta-lactamase (EC 3.5.2.6), at 76,762 to 77,934 _73 Position (kb) 75 76 77Strain fitness (log2 ratio) -2 -1 0 1 2at 75.206 kb on - strand, within Pf1N1B4_71at 75.210 kb on - strand, within Pf1N1B4_71at 75.320 kb on + strand, within Pf1N1B4_71at 75.523 kb on - strandat 75.523 kb on - strandat 75.523 kb on - strandat 75.523 kb on - strandat 75.745 kb on + strandat 75.745 kb on + strandat 75.745 kb on + strandat 75.745 kb on + strandat 75.745 kb on + strandat 75.746 kb on - strandat 75.746 kb on - strandat 75.746 kb on - strandat 75.746 kb on - strandat 75.749 kb on - strandat 75.749 kb on - strandat 75.829 kb on + strandat 76.251 kb on + strand, within Pf1N1B4_72at 76.251 kb on + strand, within Pf1N1B4_72at 76.252 kb on - strand, within Pf1N1B4_72at 76.252 kb on - strand, within Pf1N1B4_72at 76.656 kb on + strandat 76.657 kb on - strandat 76.688 kb on + strandat 76.689 kb on - strandat 76.689 kb on - strandat 76.689 kb on - strandat 76.689 kb on - strandat 76.786 kb on - strandat 76.813 kb on + strandat 76.814 kb on - strandat 76.973 kb on - strand, within Pf1N1B4_73at 76.973 kb on - strand, within Pf1N1B4_73at 77.071 kb on + strand, within Pf1N1B4_73at 77.124 kb on - strand, within Pf1N1B4_73at 77.132 kb on + strand, within Pf1N1B4_73at 77.133 kb on - strand, within Pf1N1B4_73at 77.133 kb on - strand, within Pf1N1B4_73at 77.133 kb on - strand, within Pf1N1B4_73at 77.133 kb on - strand, within Pf1N1B4_73at 77.179 kb on + strand, within Pf1N1B4_73at 77.179 kb on + strand, within Pf1N1B4_73at 77.179 kb on + strand, within Pf1N1B4_73at 77.180 kb on - strand, within Pf1N1B4_73at 77.180 kb on - strand, within Pf1N1B4_73at 77.245 kb on + strand, within Pf1N1B4_73at 77.246 kb on - strand, within Pf1N1B4_73at 77.293 kb on + strand, within Pf1N1B4_73at 77.407 kb on + strand, within Pf1N1B4_73at 77.408 kb on - strand, within Pf1N1B4_73at 77.408 kb on - strand, within Pf1N1B4_73at 77.408 kb on - strand, within Pf1N1B4_73at 77.440 kb on + strand, within Pf1N1B4_73at 77.452 kb on + strand, within Pf1N1B4_73

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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75,206 - Pf1N1B4_71 0.69 -0.5
75,210 - Pf1N1B4_71 0.69 +0.8
75,320 + Pf1N1B4_71 0.77 +1.1
75,523 - +0.4
75,523 - +0.2
75,523 - +0.8
75,523 - -0.4
75,745 + +0.0
75,745 + +0.8
75,745 + -1.5
75,745 + -0.0
75,745 + -0.8
75,746 - +1.4
75,746 - -1.0
75,746 - -0.8
75,746 - -1.7
75,749 - -0.2
75,749 - -0.8
75,829 + +0.8
76,251 + Pf1N1B4_72 0.58 +0.9
76,251 + Pf1N1B4_72 0.58 -0.2
76,252 - Pf1N1B4_72 0.58 +2.1
76,252 - Pf1N1B4_72 0.58 +0.0
76,656 + +0.9
76,657 - +0.4
76,688 + -1.2
76,689 - -0.2
76,689 - -0.2
76,689 - -1.7
76,689 - -0.2
76,786 - -0.2
76,813 + +0.2
76,814 - -0.8
76,973 - Pf1N1B4_73 0.18 +1.0
76,973 - Pf1N1B4_73 0.18 +1.0
77,071 + Pf1N1B4_73 0.26 +0.1
77,124 - Pf1N1B4_73 0.31 -0.4
77,132 + Pf1N1B4_73 0.32 +1.5
77,133 - Pf1N1B4_73 0.32 +0.9
77,133 - Pf1N1B4_73 0.32 -0.5
77,133 - Pf1N1B4_73 0.32 +0.8
77,133 - Pf1N1B4_73 0.32 +1.5
77,179 + Pf1N1B4_73 0.36 -2.2
77,179 + Pf1N1B4_73 0.36 -0.8
77,179 + Pf1N1B4_73 0.36 -0.8
77,180 - Pf1N1B4_73 0.36 -0.4
77,180 - Pf1N1B4_73 0.36 -0.2
77,245 + Pf1N1B4_73 0.41 +0.3
77,246 - Pf1N1B4_73 0.41 -0.1
77,293 + Pf1N1B4_73 0.45 +0.5
77,407 + Pf1N1B4_73 0.55 -0.5
77,408 - Pf1N1B4_73 0.55 -1.4
77,408 - Pf1N1B4_73 0.55 -0.6
77,408 - Pf1N1B4_73 0.55 +0.3
77,440 + Pf1N1B4_73 0.58 +0.8
77,452 + Pf1N1B4_73 0.59 +1.6

Or see this region's nucleotide sequence