Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5865 and Pf1N1B4_5866 are separated by 159 nucleotides Pf1N1B4_5866 and Pf1N1B4_5867 are separated by 102 nucleotides Pf1N1B4_5867 and Pf1N1B4_5868 are separated by 105 nucleotides Pf1N1B4_5868 and Pf1N1B4_5869 overlap by 4 nucleotides
Pf1N1B4_5865 - FIG00960493: hypothetical protein, at 300,238 to 301,629
_5865
Pf1N1B4_5866 - ThiJ/PfpI family protein, at 301,789 to 302,466
_5866
Pf1N1B4_5867 - hypothetical protein, at 302,569 to 302,763
_5867
Pf1N1B4_5868 - Secreted protein Hcp, at 302,869 to 303,384
_5868
Pf1N1B4_5869 - hypothetical protein, at 303,381 to 303,497
_5869
Position (kb) 301
302
303 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 300.909 kb on + strand, within Pf1N1B4_5865 at 301.234 kb on + strand, within Pf1N1B4_5865 at 301.234 kb on + strand, within Pf1N1B4_5865 at 301.393 kb on + strand, within Pf1N1B4_5865 at 301.394 kb on - strand, within Pf1N1B4_5865 at 301.394 kb on - strand, within Pf1N1B4_5865 at 301.417 kb on + strand, within Pf1N1B4_5865 at 301.418 kb on - strand, within Pf1N1B4_5865 at 301.489 kb on + strand, within Pf1N1B4_5865 at 301.562 kb on - strand at 301.641 kb on + strand at 301.878 kb on - strand, within Pf1N1B4_5866 at 302.186 kb on + strand, within Pf1N1B4_5866 at 302.227 kb on + strand, within Pf1N1B4_5866 at 302.228 kb on - strand, within Pf1N1B4_5866 at 302.372 kb on + strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.373 kb on - strand, within Pf1N1B4_5866 at 302.376 kb on - strand, within Pf1N1B4_5866 at 302.376 kb on - strand, within Pf1N1B4_5866 at 302.642 kb on - strand, within Pf1N1B4_5867 at 302.776 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.960 kb on - strand, within Pf1N1B4_5868 at 303.059 kb on + strand, within Pf1N1B4_5868 at 303.089 kb on - strand at 303.196 kb on + strand at 303.197 kb on - strand, within Pf1N1B4_5868 at 303.197 kb on - strand, within Pf1N1B4_5868 at 303.197 kb on - strand, within Pf1N1B4_5868 at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.199 kb on + strand at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.200 kb on - strand, within Pf1N1B4_5868 at 303.338 kb on + strand at 303.339 kb on - strand at 303.383 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 300,909 + Pf1N1B4_5865 0.48 -0.5 301,234 + Pf1N1B4_5865 0.72 -0.2 301,234 + Pf1N1B4_5865 0.72 -0.2 301,393 + Pf1N1B4_5865 0.83 -0.0 301,394 - Pf1N1B4_5865 0.83 -0.3 301,394 - Pf1N1B4_5865 0.83 +0.5 301,417 + Pf1N1B4_5865 0.85 -0.2 301,418 - Pf1N1B4_5865 0.85 -1.9 301,489 + Pf1N1B4_5865 0.90 -0.9 301,562 - +0.7 301,641 + -0.7 301,878 - Pf1N1B4_5866 0.13 -0.7 302,186 + Pf1N1B4_5866 0.59 -0.7 302,227 + Pf1N1B4_5866 0.65 +0.3 302,228 - Pf1N1B4_5866 0.65 +0.5 302,372 + Pf1N1B4_5866 0.86 +0.7 302,373 - Pf1N1B4_5866 0.86 -1.3 302,373 - Pf1N1B4_5866 0.86 +1.5 302,373 - Pf1N1B4_5866 0.86 -0.3 302,373 - Pf1N1B4_5866 0.86 +1.3 302,376 - Pf1N1B4_5866 0.87 -0.1 302,376 - Pf1N1B4_5866 0.87 +1.7 302,642 - Pf1N1B4_5867 0.37 +0.9 302,776 + -0.3 302,884 + +1.7 302,884 + -0.9 302,884 + +1.4 302,884 + -0.4 302,884 + +0.7 302,884 + -0.3 302,884 + -0.3 302,884 + +0.9 302,885 - -0.3 302,885 - +0.7 302,885 - +0.5 302,885 - -2.1 302,885 - +0.1 302,885 - -1.1 302,885 - +1.1 302,885 - +1.3 302,885 - -1.1 302,960 - Pf1N1B4_5868 0.18 +0.2 303,059 + Pf1N1B4_5868 0.37 +0.7 303,089 - +0.7 303,196 + +0.7 303,197 - Pf1N1B4_5868 0.64 -1.3 303,197 - Pf1N1B4_5868 0.64 -0.3 303,197 - Pf1N1B4_5868 0.64 +0.4 303,199 + Pf1N1B4_5868 0.64 -2.1 303,199 + Pf1N1B4_5868 0.64 -0.3 303,199 + +0.1 303,199 + Pf1N1B4_5868 0.64 +0.7 303,199 + Pf1N1B4_5868 0.64 -2.9 303,200 - Pf1N1B4_5868 0.64 -2.3 303,338 + -0.1 303,339 - -0.5 303,383 - -0.2
Or see this region's nucleotide sequence