Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5249 and Pf1N1B4_5250 are separated by 353 nucleotides
Pf1N1B4_5249 - Outer membrane protein A precursor, at 7,856 to 8,512
_5249
Pf1N1B4_5250 - hypothetical protein, at 8,866 to 8,994
_5250
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6.871 kb on - strand at 6.871 kb on - strand at 6.871 kb on - strand at 6.882 kb on - strand at 6.882 kb on - strand at 6.887 kb on + strand at 6.888 kb on - strand at 6.888 kb on - strand at 6.888 kb on - strand at 6.888 kb on - strand at 6.908 kb on + strand at 6.908 kb on + strand at 6.908 kb on + strand at 6.909 kb on - strand at 6.928 kb on + strand at 6.928 kb on + strand at 6.929 kb on - strand at 6.929 kb on - strand at 6.929 kb on - strand at 6.944 kb on - strand at 7.000 kb on - strand at 7.018 kb on + strand at 7.019 kb on - strand at 7.034 kb on + strand at 7.035 kb on - strand at 7.137 kb on + strand at 7.137 kb on + strand at 7.139 kb on + strand at 7.139 kb on + strand at 7.139 kb on + strand at 7.140 kb on - strand at 7.140 kb on - strand at 7.140 kb on - strand at 7.140 kb on - strand at 7.140 kb on - strand at 7.140 kb on - strand at 7.140 kb on - strand at 7.140 kb on - strand at 7.140 kb on - strand at 7.406 kb on + strand at 7.442 kb on + strand at 7.443 kb on - strand at 7.563 kb on - strand at 7.569 kb on + strand at 7.663 kb on + strand at 7.684 kb on + strand at 7.684 kb on + strand at 7.685 kb on - strand at 7.810 kb on + strand at 7.857 kb on + strand at 7.926 kb on - strand, within Pf1N1B4_5249 at 7.986 kb on + strand, within Pf1N1B4_5249 at 7.993 kb on - strand, within Pf1N1B4_5249 at 8.219 kb on + strand, within Pf1N1B4_5249 at 8.219 kb on + strand, within Pf1N1B4_5249 at 8.220 kb on - strand, within Pf1N1B4_5249 at 8.220 kb on - strand, within Pf1N1B4_5249 at 8.220 kb on - strand, within Pf1N1B4_5249 at 8.228 kb on - strand, within Pf1N1B4_5249 at 8.289 kb on - strand, within Pf1N1B4_5249 at 8.343 kb on + strand, within Pf1N1B4_5249 at 8.428 kb on - strand, within Pf1N1B4_5249 at 8.594 kb on + strand at 8.595 kb on - strand at 8.595 kb on - strand at 8.642 kb on + strand at 8.643 kb on - strand at 8.654 kb on + strand at 8.655 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.659 kb on - strand at 8.676 kb on - strand at 8.761 kb on + strand at 8.761 kb on + strand at 8.814 kb on + strand at 8.818 kb on + strand at 8.819 kb on - strand at 8.819 kb on - strand at 9.008 kb on - strand at 9.074 kb on + strand at 9.074 kb on + strand at 9.075 kb on - strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.406 kb on + strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.407 kb on - strand at 9.410 kb on + strand at 9.410 kb on + strand at 9.411 kb on - strand at 9.411 kb on - strand at 9.417 kb on + strand at 9.417 kb on + strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.418 kb on - strand at 9.422 kb on - strand at 9.422 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 6,871 - -0.6 6,871 - -0.3 6,871 - +0.0 6,882 - -0.3 6,882 - +0.0 6,887 + +1.0 6,888 - -0.3 6,888 - -1.0 6,888 - +0.6 6,888 - +0.2 6,908 + -0.2 6,908 + -2.2 6,908 + -1.3 6,909 - -0.1 6,928 + +0.0 6,928 + +0.7 6,929 - +1.3 6,929 - +1.8 6,929 - -0.6 6,944 - -0.2 7,000 - -0.9 7,018 + +2.0 7,019 - -2.4 7,034 + -1.2 7,035 - +1.0 7,137 + -0.3 7,137 + -1.6 7,139 + +0.6 7,139 + +0.3 7,139 + +1.0 7,140 - +0.0 7,140 - -1.6 7,140 - -0.1 7,140 - -1.2 7,140 - +2.2 7,140 - +0.0 7,140 - +0.0 7,140 - +2.0 7,140 - -0.4 7,406 + -0.2 7,442 + +0.4 7,443 - -0.6 7,563 - -0.1 7,569 + +0.2 7,663 + +0.2 7,684 + +0.1 7,684 + +0.3 7,685 - +0.5 7,810 + +1.0 7,857 + -0.4 7,926 - Pf1N1B4_5249 0.11 +0.4 7,986 + Pf1N1B4_5249 0.20 +1.6 7,993 - Pf1N1B4_5249 0.21 -0.2 8,219 + Pf1N1B4_5249 0.55 +0.4 8,219 + Pf1N1B4_5249 0.55 +0.4 8,220 - Pf1N1B4_5249 0.55 +0.6 8,220 - Pf1N1B4_5249 0.55 -0.6 8,220 - Pf1N1B4_5249 0.55 +1.0 8,228 - Pf1N1B4_5249 0.57 -0.3 8,289 - Pf1N1B4_5249 0.66 +1.0 8,343 + Pf1N1B4_5249 0.74 -0.8 8,428 - Pf1N1B4_5249 0.87 +0.5 8,594 + -0.8 8,595 - -0.2 8,595 - +0.5 8,642 + +1.6 8,643 - +2.0 8,654 + +0.0 8,655 - +0.4 8,659 - +1.0 8,659 - +0.4 8,659 - -0.6 8,659 - +0.0 8,659 - +1.4 8,676 - -0.6 8,761 + -0.3 8,761 + +0.0 8,814 + +1.6 8,818 + -1.0 8,819 - +1.6 8,819 - +0.4 9,008 - +1.4 9,074 + -0.7 9,074 + -1.6 9,075 - -0.6 9,406 + +0.6 9,406 + +1.6 9,406 + -0.2 9,406 + -1.6 9,406 + -0.2 9,407 - +0.4 9,407 - -0.5 9,407 - +0.3 9,407 - -1.0 9,410 + -0.2 9,410 + -1.3 9,411 - +1.8 9,411 - +0.3 9,417 + -0.1 9,417 + +0.3 9,418 - -0.1 9,418 - +0.4 9,418 - +0.2 9,418 - +0.2 9,418 - -1.0 9,418 - +0.4 9,418 - -1.3 9,418 - -0.7 9,418 - +0.7 9,418 - +0.8 9,418 - -0.5 9,422 - -0.4 9,422 - -1.4
Or see this region's nucleotide sequence