Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5158 and Pf1N1B4_5159 overlap by 25 nucleotides
Pf1N1B4_5158 - Endoglucanase precursor (EC 3.2.1.4), at 5,748,820 to 5,750,061
_5158
Pf1N1B4_5159 - Cellulose synthase operon protein C, at 5,750,037 to 5,753,897
_5159
Position (kb)
5750
5751
5752
5753
5754 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5749.435 kb on + strand, within Pf1N1B4_5158 at 5749.435 kb on + strand, within Pf1N1B4_5158 at 5749.436 kb on - strand, within Pf1N1B4_5158 at 5749.633 kb on + strand, within Pf1N1B4_5158 at 5749.634 kb on - strand, within Pf1N1B4_5158 at 5749.634 kb on - strand, within Pf1N1B4_5158 at 5749.651 kb on + strand, within Pf1N1B4_5158 at 5749.674 kb on + strand, within Pf1N1B4_5158 at 5749.942 kb on + strand at 5749.942 kb on + strand at 5749.943 kb on - strand at 5749.943 kb on - strand at 5749.943 kb on - strand at 5750.126 kb on - strand at 5750.232 kb on + strand at 5750.493 kb on + strand, within Pf1N1B4_5159 at 5750.494 kb on - strand at 5750.764 kb on - strand, within Pf1N1B4_5159 at 5751.261 kb on + strand, within Pf1N1B4_5159 at 5751.262 kb on - strand, within Pf1N1B4_5159 at 5751.636 kb on + strand, within Pf1N1B4_5159 at 5751.637 kb on - strand, within Pf1N1B4_5159 at 5751.637 kb on - strand, within Pf1N1B4_5159 at 5751.637 kb on - strand at 5751.889 kb on - strand, within Pf1N1B4_5159 at 5752.156 kb on - strand, within Pf1N1B4_5159 at 5752.279 kb on + strand at 5752.803 kb on + strand, within Pf1N1B4_5159 at 5752.803 kb on + strand, within Pf1N1B4_5159 at 5752.804 kb on - strand, within Pf1N1B4_5159 at 5752.804 kb on - strand at 5753.110 kb on - strand, within Pf1N1B4_5159 at 5753.484 kb on + strand at 5753.493 kb on + strand, within Pf1N1B4_5159 at 5753.494 kb on - strand, within Pf1N1B4_5159 at 5753.733 kb on + strand at 5753.733 kb on + strand at 5753.968 kb on + strand at 5753.969 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,749,435 + Pf1N1B4_5158 0.50 +0.8 5,749,435 + Pf1N1B4_5158 0.50 -0.2 5,749,436 - Pf1N1B4_5158 0.50 +0.4 5,749,633 + Pf1N1B4_5158 0.65 +2.2 5,749,634 - Pf1N1B4_5158 0.66 +0.4 5,749,634 - Pf1N1B4_5158 0.66 +0.4 5,749,651 + Pf1N1B4_5158 0.67 +3.0 5,749,674 + Pf1N1B4_5158 0.69 +0.4 5,749,942 + +0.4 5,749,942 + +0.4 5,749,943 - -0.6 5,749,943 - -1.2 5,749,943 - -1.2 5,750,126 - -1.0 5,750,232 + +1.4 5,750,493 + Pf1N1B4_5159 0.12 -2.2 5,750,494 - +0.4 5,750,764 - Pf1N1B4_5159 0.19 -0.5 5,751,261 + Pf1N1B4_5159 0.32 -0.8 5,751,262 - Pf1N1B4_5159 0.32 -1.4 5,751,636 + Pf1N1B4_5159 0.41 -0.6 5,751,637 - Pf1N1B4_5159 0.41 +0.5 5,751,637 - Pf1N1B4_5159 0.41 -1.9 5,751,637 - +0.4 5,751,889 - Pf1N1B4_5159 0.48 +0.4 5,752,156 - Pf1N1B4_5159 0.55 -1.5 5,752,279 + +0.4 5,752,803 + Pf1N1B4_5159 0.72 +0.4 5,752,803 + Pf1N1B4_5159 0.72 -0.8 5,752,804 - Pf1N1B4_5159 0.72 -0.3 5,752,804 - -0.2 5,753,110 - Pf1N1B4_5159 0.80 -1.1 5,753,484 + +0.4 5,753,493 + Pf1N1B4_5159 0.90 +1.4 5,753,494 - Pf1N1B4_5159 0.90 -0.9 5,753,733 + +0.7 5,753,733 + -0.6 5,753,968 + +0.4 5,753,969 - +0.4
Or see this region's nucleotide sequence