Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5099 and Pf1N1B4_5100 are separated by 93 nucleotides Pf1N1B4_5100 and Pf1N1B4_5101 are separated by 180 nucleotides
Pf1N1B4_5099 - Proline iminopeptidase (EC 3.4.11.5), at 5,652,893 to 5,653,837
_5099
Pf1N1B4_5100 - Transcriptional regulator ahyR/asaR family, at 5,653,931 to 5,654,689
_5100
Pf1N1B4_5101 - Proline iminopeptidase (EC 3.4.11.5), at 5,654,870 to 5,655,757
_5101
Position (kb)
5653
5654
5655 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5653.207 kb on - strand, within Pf1N1B4_5099 at 5653.241 kb on - strand, within Pf1N1B4_5099 at 5653.528 kb on - strand, within Pf1N1B4_5099 at 5653.689 kb on + strand, within Pf1N1B4_5099 at 5653.690 kb on - strand at 5653.690 kb on - strand, within Pf1N1B4_5099 at 5653.690 kb on - strand, within Pf1N1B4_5099 at 5653.690 kb on - strand, within Pf1N1B4_5099 at 5653.702 kb on - strand, within Pf1N1B4_5099 at 5653.898 kb on + strand at 5654.095 kb on + strand, within Pf1N1B4_5100 at 5654.125 kb on - strand, within Pf1N1B4_5100 at 5654.138 kb on - strand, within Pf1N1B4_5100 at 5654.335 kb on - strand, within Pf1N1B4_5100 at 5654.401 kb on - strand, within Pf1N1B4_5100 at 5654.436 kb on - strand, within Pf1N1B4_5100 at 5654.733 kb on + strand at 5654.786 kb on - strand at 5654.786 kb on - strand at 5654.938 kb on + strand at 5654.938 kb on + strand at 5655.002 kb on + strand, within Pf1N1B4_5101 at 5655.002 kb on + strand at 5655.225 kb on - strand, within Pf1N1B4_5101
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,653,207 - Pf1N1B4_5099 0.33 -0.3 5,653,241 - Pf1N1B4_5099 0.37 -0.8 5,653,528 - Pf1N1B4_5099 0.67 +0.4 5,653,689 + Pf1N1B4_5099 0.84 -0.2 5,653,690 - -1.2 5,653,690 - Pf1N1B4_5099 0.84 +1.4 5,653,690 - Pf1N1B4_5099 0.84 +0.1 5,653,690 - Pf1N1B4_5099 0.84 +1.0 5,653,702 - Pf1N1B4_5099 0.86 +1.4 5,653,898 + -0.2 5,654,095 + Pf1N1B4_5100 0.22 -2.9 5,654,125 - Pf1N1B4_5100 0.26 -1.9 5,654,138 - Pf1N1B4_5100 0.27 -1.6 5,654,335 - Pf1N1B4_5100 0.53 -1.2 5,654,401 - Pf1N1B4_5100 0.62 +0.4 5,654,436 - Pf1N1B4_5100 0.67 -2.8 5,654,733 + +0.3 5,654,786 - +0.7 5,654,786 - -1.9 5,654,938 + +0.7 5,654,938 + +0.7 5,655,002 + Pf1N1B4_5101 0.15 +1.8 5,655,002 + +1.7 5,655,225 - Pf1N1B4_5101 0.40 -0.0
Or see this region's nucleotide sequence