Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4895 and Pf1N1B4_4896 are separated by 133 nucleotides Pf1N1B4_4896 and Pf1N1B4_4897 are separated by 224 nucleotides
Pf1N1B4_4895 - Putrescine ABC transporter, periplasmic putrescine-binding protein, at 5,416,997 to 5,418,082
_4895
Pf1N1B4_4896 - N-ethylmaleimide reductase, at 5,418,216 to 5,419,238
_4896
Pf1N1B4_4897 - Transcriptional regulator, LysR family, at 5,419,463 to 5,420,347
_4897
Position (kb)
5418
5419
5420 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5417.369 kb on + strand, within Pf1N1B4_4895 at 5417.369 kb on + strand, within Pf1N1B4_4895 at 5417.550 kb on - strand, within Pf1N1B4_4895 at 5417.550 kb on - strand, within Pf1N1B4_4895 at 5417.550 kb on - strand, within Pf1N1B4_4895 at 5417.627 kb on + strand, within Pf1N1B4_4895 at 5417.628 kb on - strand, within Pf1N1B4_4895 at 5417.655 kb on - strand, within Pf1N1B4_4895 at 5417.861 kb on + strand, within Pf1N1B4_4895 at 5417.862 kb on - strand, within Pf1N1B4_4895 at 5417.862 kb on - strand, within Pf1N1B4_4895 at 5417.897 kb on - strand, within Pf1N1B4_4895 at 5417.900 kb on + strand, within Pf1N1B4_4895 at 5417.900 kb on + strand, within Pf1N1B4_4895 at 5417.901 kb on - strand, within Pf1N1B4_4895 at 5418.185 kb on + strand at 5418.186 kb on - strand at 5418.445 kb on + strand, within Pf1N1B4_4896 at 5418.445 kb on + strand, within Pf1N1B4_4896 at 5418.445 kb on + strand, within Pf1N1B4_4896 at 5418.446 kb on - strand at 5418.456 kb on + strand, within Pf1N1B4_4896 at 5418.457 kb on - strand, within Pf1N1B4_4896 at 5418.457 kb on - strand, within Pf1N1B4_4896 at 5418.510 kb on + strand, within Pf1N1B4_4896 at 5418.539 kb on - strand, within Pf1N1B4_4896 at 5418.558 kb on + strand, within Pf1N1B4_4896 at 5418.608 kb on + strand, within Pf1N1B4_4896 at 5418.681 kb on + strand, within Pf1N1B4_4896 at 5418.682 kb on - strand, within Pf1N1B4_4896 at 5418.727 kb on + strand, within Pf1N1B4_4896 at 5418.728 kb on - strand, within Pf1N1B4_4896 at 5418.728 kb on - strand, within Pf1N1B4_4896 at 5418.738 kb on - strand, within Pf1N1B4_4896 at 5418.984 kb on + strand at 5419.016 kb on - strand, within Pf1N1B4_4896 at 5419.096 kb on + strand, within Pf1N1B4_4896 at 5419.096 kb on + strand, within Pf1N1B4_4896 at 5419.096 kb on + strand, within Pf1N1B4_4896 at 5419.097 kb on - strand, within Pf1N1B4_4896 at 5419.097 kb on - strand, within Pf1N1B4_4896 at 5419.097 kb on - strand at 5419.179 kb on - strand at 5419.179 kb on - strand at 5419.179 kb on - strand at 5419.189 kb on + strand at 5419.190 kb on - strand at 5419.192 kb on + strand at 5419.287 kb on + strand at 5419.288 kb on - strand at 5419.301 kb on + strand at 5419.329 kb on + strand at 5419.330 kb on - strand at 5419.330 kb on - strand at 5419.354 kb on + strand at 5419.361 kb on - strand at 5419.441 kb on + strand at 5419.600 kb on - strand, within Pf1N1B4_4897 at 5419.600 kb on - strand at 5419.762 kb on - strand at 5420.145 kb on - strand at 5420.145 kb on - strand, within Pf1N1B4_4897
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,417,369 + Pf1N1B4_4895 0.34 -0.0 5,417,369 + Pf1N1B4_4895 0.34 +0.7 5,417,550 - Pf1N1B4_4895 0.51 +0.6 5,417,550 - Pf1N1B4_4895 0.51 -2.0 5,417,550 - Pf1N1B4_4895 0.51 +0.4 5,417,627 + Pf1N1B4_4895 0.58 +0.6 5,417,628 - Pf1N1B4_4895 0.58 -0.0 5,417,655 - Pf1N1B4_4895 0.61 +1.2 5,417,861 + Pf1N1B4_4895 0.80 -0.6 5,417,862 - Pf1N1B4_4895 0.80 +0.5 5,417,862 - Pf1N1B4_4895 0.80 +0.4 5,417,897 - Pf1N1B4_4895 0.83 -1.5 5,417,900 + Pf1N1B4_4895 0.83 -0.6 5,417,900 + Pf1N1B4_4895 0.83 -0.8 5,417,901 - Pf1N1B4_4895 0.83 +1.1 5,418,185 + +0.1 5,418,186 - -0.4 5,418,445 + Pf1N1B4_4896 0.22 +0.9 5,418,445 + Pf1N1B4_4896 0.22 +1.4 5,418,445 + Pf1N1B4_4896 0.22 +1.0 5,418,446 - +1.7 5,418,456 + Pf1N1B4_4896 0.23 +0.4 5,418,457 - Pf1N1B4_4896 0.24 +0.7 5,418,457 - Pf1N1B4_4896 0.24 +0.4 5,418,510 + Pf1N1B4_4896 0.29 -0.6 5,418,539 - Pf1N1B4_4896 0.32 -0.1 5,418,558 + Pf1N1B4_4896 0.33 +0.4 5,418,608 + Pf1N1B4_4896 0.38 +0.4 5,418,681 + Pf1N1B4_4896 0.45 -1.2 5,418,682 - Pf1N1B4_4896 0.46 -0.6 5,418,727 + Pf1N1B4_4896 0.50 +1.1 5,418,728 - Pf1N1B4_4896 0.50 -0.2 5,418,728 - Pf1N1B4_4896 0.50 +0.8 5,418,738 - Pf1N1B4_4896 0.51 -3.1 5,418,984 + -2.4 5,419,016 - Pf1N1B4_4896 0.78 +1.2 5,419,096 + Pf1N1B4_4896 0.86 +0.1 5,419,096 + Pf1N1B4_4896 0.86 -0.8 5,419,096 + Pf1N1B4_4896 0.86 +0.1 5,419,097 - Pf1N1B4_4896 0.86 +0.5 5,419,097 - Pf1N1B4_4896 0.86 +1.2 5,419,097 - +0.4 5,419,179 - +0.0 5,419,179 - -2.0 5,419,179 - +0.4 5,419,189 + -2.2 5,419,190 - -0.1 5,419,192 + -1.2 5,419,287 + -0.8 5,419,288 - -0.4 5,419,301 + -2.4 5,419,329 + +1.6 5,419,330 - -0.2 5,419,330 - -0.6 5,419,354 + -0.1 5,419,361 - -1.2 5,419,441 + -1.0 5,419,600 - Pf1N1B4_4897 0.15 -0.9 5,419,600 - -0.6 5,419,762 - +0.4 5,420,145 - -0.0 5,420,145 - Pf1N1B4_4897 0.77 +0.4
Or see this region's nucleotide sequence