Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4800 and Pf1N1B4_4801 overlap by 4 nucleotides Pf1N1B4_4801 and Pf1N1B4_4802 overlap by 8 nucleotides Pf1N1B4_4802 and Pf1N1B4_4803 are separated by 402 nucleotides
Pf1N1B4_4800 - Immune-responsive protein 1, at 5,302,728 to 5,304,134
_4800
Pf1N1B4_4801 - Polysaccharide deacetylase, at 5,304,131 to 5,305,018
_4801
Pf1N1B4_4802 - polysaccharide deacetylase, at 5,305,011 to 5,305,859
_4802
Pf1N1B4_4803 - ABC-type polar amino acid transport system protein, substrate-binding protein, at 5,306,262 to 5,307,047
_4803
Position (kb) 5305
5306 Strain fitness (log2 ratio) -1
0
1
2 at 5304.098 kb on - strand at 5304.141 kb on + strand at 5304.162 kb on + strand at 5304.246 kb on - strand, within Pf1N1B4_4801 at 5304.339 kb on + strand, within Pf1N1B4_4801 at 5304.339 kb on + strand, within Pf1N1B4_4801 at 5304.394 kb on + strand, within Pf1N1B4_4801 at 5304.403 kb on - strand, within Pf1N1B4_4801 at 5304.715 kb on - strand, within Pf1N1B4_4801 at 5304.957 kb on + strand at 5305.229 kb on - strand, within Pf1N1B4_4802 at 5305.291 kb on + strand, within Pf1N1B4_4802 at 5305.291 kb on + strand, within Pf1N1B4_4802 at 5305.361 kb on - strand at 5305.394 kb on - strand, within Pf1N1B4_4802 at 5305.735 kb on + strand at 5305.874 kb on - strand at 5305.877 kb on + strand at 5305.877 kb on + strand at 5305.878 kb on - strand at 5305.878 kb on - strand at 5305.879 kb on + strand at 5305.880 kb on - strand at 5305.883 kb on + strand at 5306.025 kb on - strand at 5306.088 kb on - strand at 5306.208 kb on + strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.209 kb on - strand at 5306.263 kb on + strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.264 kb on - strand at 5306.455 kb on + strand, within Pf1N1B4_4803 at 5306.456 kb on - strand, within Pf1N1B4_4803 at 5306.456 kb on - strand, within Pf1N1B4_4803 at 5306.456 kb on - strand, within Pf1N1B4_4803 at 5306.744 kb on - strand, within Pf1N1B4_4803
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,304,098 - +0.4 5,304,141 + +1.4 5,304,162 + -1.6 5,304,246 - Pf1N1B4_4801 0.13 +0.4 5,304,339 + Pf1N1B4_4801 0.23 +0.9 5,304,339 + Pf1N1B4_4801 0.23 -0.6 5,304,394 + Pf1N1B4_4801 0.30 +0.8 5,304,403 - Pf1N1B4_4801 0.31 +0.6 5,304,715 - Pf1N1B4_4801 0.66 +0.7 5,304,957 + -1.5 5,305,229 - Pf1N1B4_4802 0.26 -0.2 5,305,291 + Pf1N1B4_4802 0.33 -1.7 5,305,291 + Pf1N1B4_4802 0.33 +0.4 5,305,361 - +1.4 5,305,394 - Pf1N1B4_4802 0.45 -1.6 5,305,735 + +1.0 5,305,874 - -0.7 5,305,877 + -0.8 5,305,877 + -0.3 5,305,878 - -1.2 5,305,878 - -0.4 5,305,879 + +0.2 5,305,880 - -0.4 5,305,883 + -1.6 5,306,025 - +0.1 5,306,088 - +2.6 5,306,208 + -1.4 5,306,209 - -0.2 5,306,209 - +1.0 5,306,209 - +1.4 5,306,209 - -0.1 5,306,209 - +0.6 5,306,209 - +0.6 5,306,263 + -0.3 5,306,264 - +0.7 5,306,264 - +1.0 5,306,264 - +0.8 5,306,264 - +1.0 5,306,264 - -1.4 5,306,264 - -0.3 5,306,455 + Pf1N1B4_4803 0.25 -0.4 5,306,456 - Pf1N1B4_4803 0.25 -1.0 5,306,456 - Pf1N1B4_4803 0.25 +0.1 5,306,456 - Pf1N1B4_4803 0.25 +0.7 5,306,744 - Pf1N1B4_4803 0.61 -1.3
Or see this region's nucleotide sequence