Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4759 and Pf1N1B4_4760 are separated by 391 nucleotides Pf1N1B4_4760 and Pf1N1B4_4761 overlap by 4 nucleotides Pf1N1B4_4761 and Pf1N1B4_4762 are separated by 64 nucleotides Pf1N1B4_4762 and Pf1N1B4_4763 are separated by 125 nucleotides
Pf1N1B4_4759 - Proteinase inhibitor I11, ecotin precursor, at 5,257,201 to 5,257,683
_4759
Pf1N1B4_4760 - FIG00962118: hypothetical protein, at 5,258,075 to 5,258,344
_4760
Pf1N1B4_4761 - hypothetical protein, at 5,258,341 to 5,258,739
_4761
Pf1N1B4_4762 - hypothetical protein, at 5,258,804 to 5,259,070
_4762
Pf1N1B4_4763 - 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43), at 5,259,196 to 5,260,221
_4763
Position (kb) 5258
5259 Strain fitness (log2 ratio) -2
-1
0
1 at 5257.645 kb on + strand at 5257.901 kb on + strand at 5258.097 kb on + strand at 5258.180 kb on + strand, within Pf1N1B4_4760 at 5258.226 kb on + strand at 5258.503 kb on - strand, within Pf1N1B4_4761 at 5258.503 kb on - strand, within Pf1N1B4_4761 at 5258.558 kb on + strand, within Pf1N1B4_4761 at 5258.800 kb on - strand at 5258.814 kb on + strand at 5258.815 kb on - strand at 5258.815 kb on - strand at 5258.815 kb on - strand at 5258.815 kb on - strand at 5258.815 kb on - strand at 5258.818 kb on + strand at 5258.819 kb on - strand at 5258.842 kb on - strand, within Pf1N1B4_4762 at 5258.845 kb on + strand, within Pf1N1B4_4762 at 5258.846 kb on - strand, within Pf1N1B4_4762 at 5258.877 kb on + strand, within Pf1N1B4_4762 at 5258.877 kb on + strand, within Pf1N1B4_4762 at 5258.878 kb on - strand, within Pf1N1B4_4762 at 5258.878 kb on - strand, within Pf1N1B4_4762 at 5258.965 kb on - strand, within Pf1N1B4_4762 at 5259.208 kb on + strand at 5259.313 kb on - strand, within Pf1N1B4_4763 at 5259.702 kb on - strand, within Pf1N1B4_4763
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,257,645 + +1.4 5,257,901 + -2.4 5,258,097 + +0.1 5,258,180 + Pf1N1B4_4760 0.39 -0.6 5,258,226 + -0.0 5,258,503 - Pf1N1B4_4761 0.41 -0.5 5,258,503 - Pf1N1B4_4761 0.41 -0.6 5,258,558 + Pf1N1B4_4761 0.54 -1.4 5,258,800 - -0.8 5,258,814 + -0.6 5,258,815 - +0.1 5,258,815 - -1.6 5,258,815 - -0.1 5,258,815 - +0.2 5,258,815 - +0.4 5,258,818 + -0.8 5,258,819 - +0.4 5,258,842 - Pf1N1B4_4762 0.14 -0.4 5,258,845 + Pf1N1B4_4762 0.15 +0.2 5,258,846 - Pf1N1B4_4762 0.16 -0.6 5,258,877 + Pf1N1B4_4762 0.27 -0.6 5,258,877 + Pf1N1B4_4762 0.27 +0.7 5,258,878 - Pf1N1B4_4762 0.28 +1.2 5,258,878 - Pf1N1B4_4762 0.28 +1.6 5,258,965 - Pf1N1B4_4762 0.60 +0.0 5,259,208 + -0.6 5,259,313 - Pf1N1B4_4763 0.11 +0.3 5,259,702 - Pf1N1B4_4763 0.49 +0.7
Or see this region's nucleotide sequence