Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4699 and Pf1N1B4_4700 are separated by 591 nucleotides Pf1N1B4_4700 and Pf1N1B4_4701 are separated by 19 nucleotides Pf1N1B4_4701 and Pf1N1B4_4702 are separated by 234 nucleotides
Pf1N1B4_4699 - Multidrug translocase MdfA, at 5,192,584 to 5,193,825
_4699
Pf1N1B4_4700 - FIG00956898: hypothetical protein, at 5,194,417 to 5,194,605
_4700
Pf1N1B4_4701 - Fe2+/Zn2+ uptake regulation proteins, at 5,194,625 to 5,194,996
_4701
Pf1N1B4_4702 - Acyl-homoserine lactone acylase PvdQ (EC 3.5.1.-), quorum-quenching, at 5,195,231 to 5,197,576
_4702
Position (kb)
5194
5195 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5193.651 kb on - strand, within Pf1N1B4_4699 at 5193.743 kb on + strand at 5193.744 kb on - strand at 5193.802 kb on + strand at 5193.803 kb on - strand at 5193.803 kb on - strand at 5193.804 kb on + strand at 5193.805 kb on - strand at 5193.809 kb on - strand at 5194.049 kb on + strand at 5194.050 kb on - strand at 5194.094 kb on + strand at 5194.116 kb on + strand at 5194.534 kb on + strand, within Pf1N1B4_4700 at 5194.534 kb on + strand, within Pf1N1B4_4700 at 5194.992 kb on + strand at 5194.992 kb on + strand at 5194.993 kb on - strand at 5194.993 kb on - strand at 5194.993 kb on - strand at 5195.076 kb on + strand at 5195.076 kb on + strand at 5195.077 kb on - strand at 5195.089 kb on + strand at 5195.094 kb on - strand at 5195.148 kb on + strand at 5195.149 kb on - strand at 5195.206 kb on + strand at 5195.414 kb on + strand at 5195.415 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 5,193,651 - Pf1N1B4_4699 0.86 -0.6 5,193,743 + +1.1 5,193,744 - -0.7 5,193,802 + -0.6 5,193,803 - -0.2 5,193,803 - +1.0 5,193,804 + -0.0 5,193,805 - -0.8 5,193,809 - +0.7 5,194,049 + -0.2 5,194,050 - +0.3 5,194,094 + -0.0 5,194,116 + +1.3 5,194,534 + Pf1N1B4_4700 0.62 -0.3 5,194,534 + Pf1N1B4_4700 0.62 -0.7 5,194,992 + +0.4 5,194,992 + +0.1 5,194,993 - +0.4 5,194,993 - +0.6 5,194,993 - -0.2 5,195,076 + +2.0 5,195,076 + +1.0 5,195,077 - -2.6 5,195,089 + +0.4 5,195,094 - -0.9 5,195,148 + +2.0 5,195,149 - -0.2 5,195,206 + -0.6 5,195,414 + +0.4 5,195,415 - -0.2
Or see this region's nucleotide sequence