Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4480 and Pf1N1B4_4481 are separated by 185 nucleotides Pf1N1B4_4481 and Pf1N1B4_4482 are separated by 128 nucleotides Pf1N1B4_4482 and Pf1N1B4_4483 are separated by 104 nucleotides Pf1N1B4_4483 and Pf1N1B4_4484 are separated by 183 nucleotides
Pf1N1B4_4480 - Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4), at 4,941,511 to 4,942,746
_4480
Pf1N1B4_4481 - Transcriptional regulator BkdR of isoleucine and valine catabolism operon, at 4,942,932 to 4,943,420
_4481
Pf1N1B4_4482 - FIG146518: Zn-dependent hydrolases, including glyoxylases, at 4,943,549 to 4,944,412
_4482
Pf1N1B4_4483 - Transcriptional regulator, ArsR family, at 4,944,517 to 4,944,852
_4483
Pf1N1B4_4484 - FIG002984: FAD-dependent pyridine nucleotide-disulphide oxidoreductase, at 4,945,036 to 4,946,280
_4484
Position (kb)
4943
4944
4945 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4942.668 kb on - strand at 4942.753 kb on + strand at 4942.753 kb on + strand at 4942.753 kb on + strand at 4942.779 kb on - strand at 4942.779 kb on - strand at 4942.851 kb on - strand at 4942.904 kb on - strand at 4943.226 kb on + strand, within Pf1N1B4_4481 at 4943.251 kb on - strand, within Pf1N1B4_4481 at 4943.251 kb on - strand at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.370 kb on + strand, within Pf1N1B4_4481 at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.372 kb on + strand at 4943.550 kb on + strand at 4943.550 kb on + strand at 4943.578 kb on - strand at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.809 kb on - strand, within Pf1N1B4_4482 at 4943.958 kb on + strand, within Pf1N1B4_4482 at 4943.959 kb on - strand, within Pf1N1B4_4482 at 4943.959 kb on - strand, within Pf1N1B4_4482 at 4943.959 kb on - strand, within Pf1N1B4_4482 at 4943.978 kb on - strand, within Pf1N1B4_4482 at 4944.303 kb on + strand, within Pf1N1B4_4482 at 4944.351 kb on + strand at 4944.352 kb on - strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.360 kb on + strand at 4944.361 kb on - strand at 4944.366 kb on + strand at 4944.367 kb on - strand at 4944.367 kb on - strand at 4944.465 kb on + strand at 4944.465 kb on + strand at 4944.466 kb on - strand at 4944.735 kb on - strand, within Pf1N1B4_4483 at 4944.757 kb on + strand, within Pf1N1B4_4483 at 4944.757 kb on + strand, within Pf1N1B4_4483 at 4944.757 kb on + strand, within Pf1N1B4_4483 at 4944.758 kb on - strand, within Pf1N1B4_4483 at 4944.758 kb on - strand at 4944.758 kb on - strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.761 kb on + strand, within Pf1N1B4_4483 at 4944.762 kb on - strand, within Pf1N1B4_4483 at 4944.766 kb on + strand, within Pf1N1B4_4483 at 4944.766 kb on + strand, within Pf1N1B4_4483 at 4944.767 kb on - strand, within Pf1N1B4_4483 at 4944.820 kb on + strand at 4944.821 kb on - strand at 4944.977 kb on + strand at 4945.021 kb on - strand at 4945.083 kb on - strand at 4945.085 kb on + strand at 4945.086 kb on - strand at 4945.180 kb on + strand, within Pf1N1B4_4484 at 4945.180 kb on + strand, within Pf1N1B4_4484 at 4945.180 kb on + strand, within Pf1N1B4_4484 at 4945.184 kb on - strand, within Pf1N1B4_4484 at 4945.220 kb on - strand, within Pf1N1B4_4484 at 4945.220 kb on - strand, within Pf1N1B4_4484
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,942,668 - +1.1 4,942,753 + +0.5 4,942,753 + +1.1 4,942,753 + -0.1 4,942,779 - -0.5 4,942,779 - -2.3 4,942,851 - -0.1 4,942,904 - +0.1 4,943,226 + Pf1N1B4_4481 0.60 +0.5 4,943,251 - Pf1N1B4_4481 0.65 +1.2 4,943,251 - -0.5 4,943,370 + Pf1N1B4_4481 0.90 -0.5 4,943,370 + Pf1N1B4_4481 0.90 +0.8 4,943,370 + Pf1N1B4_4481 0.90 +0.5 4,943,372 + -3.3 4,943,372 + +0.5 4,943,372 + -0.3 4,943,372 + +0.3 4,943,550 + +0.5 4,943,550 + -0.5 4,943,578 - -0.8 4,943,809 - Pf1N1B4_4482 0.30 -1.1 4,943,809 - Pf1N1B4_4482 0.30 +1.2 4,943,809 - Pf1N1B4_4482 0.30 -1.8 4,943,809 - Pf1N1B4_4482 0.30 -2.1 4,943,958 + Pf1N1B4_4482 0.47 +0.2 4,943,959 - Pf1N1B4_4482 0.47 -0.9 4,943,959 - Pf1N1B4_4482 0.47 -0.1 4,943,959 - Pf1N1B4_4482 0.47 -1.3 4,943,978 - Pf1N1B4_4482 0.50 +1.1 4,944,303 + Pf1N1B4_4482 0.87 -0.8 4,944,351 + +0.1 4,944,352 - +0.7 4,944,360 + +0.7 4,944,360 + -0.5 4,944,360 + -0.5 4,944,360 + +1.2 4,944,361 - -0.5 4,944,366 + +0.2 4,944,367 - +0.3 4,944,367 - -1.1 4,944,465 + +0.2 4,944,465 + +0.5 4,944,466 - -0.7 4,944,735 - Pf1N1B4_4483 0.65 +0.5 4,944,757 + Pf1N1B4_4483 0.71 +0.8 4,944,757 + Pf1N1B4_4483 0.71 -0.0 4,944,757 + Pf1N1B4_4483 0.71 +0.5 4,944,758 - Pf1N1B4_4483 0.72 -1.8 4,944,758 - -1.1 4,944,758 - Pf1N1B4_4483 0.72 -0.5 4,944,761 + Pf1N1B4_4483 0.73 +1.5 4,944,761 + Pf1N1B4_4483 0.73 -0.1 4,944,761 + Pf1N1B4_4483 0.73 +0.1 4,944,761 + Pf1N1B4_4483 0.73 +1.5 4,944,761 + Pf1N1B4_4483 0.73 +1.2 4,944,761 + Pf1N1B4_4483 0.73 -0.3 4,944,761 + Pf1N1B4_4483 0.73 -0.9 4,944,762 - Pf1N1B4_4483 0.73 -0.5 4,944,766 + Pf1N1B4_4483 0.74 +1.8 4,944,766 + Pf1N1B4_4483 0.74 -1.4 4,944,767 - Pf1N1B4_4483 0.74 +0.5 4,944,820 + -0.1 4,944,821 - +0.2 4,944,977 + -1.5 4,945,021 - -0.7 4,945,083 - -1.1 4,945,085 + +0.0 4,945,086 - -0.3 4,945,180 + Pf1N1B4_4484 0.12 +0.3 4,945,180 + Pf1N1B4_4484 0.12 -2.3 4,945,180 + Pf1N1B4_4484 0.12 +0.5 4,945,184 - Pf1N1B4_4484 0.12 +0.1 4,945,220 - Pf1N1B4_4484 0.15 +0.1 4,945,220 - Pf1N1B4_4484 0.15 -1.8
Or see this region's nucleotide sequence