Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4221 and Pf1N1B4_4222 are separated by 19 nucleotides Pf1N1B4_4222 and Pf1N1B4_4223 are separated by 6 nucleotides Pf1N1B4_4223 and Pf1N1B4_4224 are separated by 306 nucleotides Pf1N1B4_4224 and Pf1N1B4_4225 are separated by 149 nucleotides
Pf1N1B4_4221 - hypothetical protein, at 4,678,383 to 4,678,499
_4221
Pf1N1B4_4222 - putative tyrosinase, at 4,678,519 to 4,679,379
_4222
Pf1N1B4_4223 - Transcriptional regulator, at 4,679,386 to 4,680,279
_4223
Pf1N1B4_4224 - putative tautomerase, at 4,680,586 to 4,680,795
_4224
Pf1N1B4_4225 - transcriptional regulator, AraC family, at 4,680,945 to 4,681,907
_4225
Position (kb)
4679
4680
4681 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.497 kb on + strand at 4678.498 kb on - strand at 4678.531 kb on + strand at 4678.604 kb on - strand at 4678.604 kb on - strand at 4678.846 kb on + strand, within Pf1N1B4_4222 at 4678.846 kb on + strand, within Pf1N1B4_4222 at 4679.011 kb on + strand, within Pf1N1B4_4222 at 4679.012 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.027 kb on - strand, within Pf1N1B4_4222 at 4679.098 kb on - strand, within Pf1N1B4_4222 at 4679.230 kb on + strand, within Pf1N1B4_4222 at 4679.231 kb on - strand at 4679.234 kb on + strand, within Pf1N1B4_4222 at 4679.234 kb on + strand, within Pf1N1B4_4222 at 4679.235 kb on - strand, within Pf1N1B4_4222 at 4679.235 kb on - strand, within Pf1N1B4_4222 at 4679.290 kb on + strand, within Pf1N1B4_4222 at 4679.290 kb on + strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.291 kb on - strand, within Pf1N1B4_4222 at 4679.335 kb on + strand at 4679.336 kb on - strand at 4679.345 kb on - strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.347 kb on + strand at 4679.348 kb on - strand at 4679.348 kb on - strand at 4679.348 kb on - strand at 4679.353 kb on + strand at 4679.377 kb on + strand at 4679.377 kb on + strand at 4679.501 kb on + strand, within Pf1N1B4_4223 at 4679.502 kb on - strand at 4679.734 kb on + strand, within Pf1N1B4_4223 at 4679.734 kb on + strand, within Pf1N1B4_4223 at 4679.807 kb on + strand, within Pf1N1B4_4223 at 4679.884 kb on - strand at 4679.933 kb on + strand at 4679.934 kb on - strand, within Pf1N1B4_4223 at 4679.934 kb on - strand, within Pf1N1B4_4223 at 4680.137 kb on - strand, within Pf1N1B4_4223 at 4680.244 kb on - strand at 4680.303 kb on + strand at 4680.380 kb on - strand at 4680.403 kb on - strand at 4680.433 kb on + strand at 4680.433 kb on + strand at 4680.437 kb on + strand at 4680.437 kb on + strand at 4680.438 kb on - strand at 4680.542 kb on + strand at 4680.793 kb on + strand at 4680.946 kb on + strand at 4680.946 kb on + strand at 4680.946 kb on + strand at 4680.947 kb on - strand at 4680.982 kb on + strand at 4680.982 kb on + strand at 4681.083 kb on + strand at 4681.083 kb on + strand, within Pf1N1B4_4225
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,678,497 + -1.4 4,678,497 + +1.1 4,678,497 + -0.3 4,678,497 + -0.8 4,678,498 - +0.2 4,678,531 + -0.6 4,678,604 - -0.8 4,678,604 - +0.6 4,678,846 + Pf1N1B4_4222 0.38 +0.3 4,678,846 + Pf1N1B4_4222 0.38 +0.3 4,679,011 + Pf1N1B4_4222 0.57 -1.2 4,679,012 - Pf1N1B4_4222 0.57 -1.1 4,679,027 - Pf1N1B4_4222 0.59 -0.3 4,679,027 - Pf1N1B4_4222 0.59 -1.8 4,679,027 - Pf1N1B4_4222 0.59 -0.6 4,679,098 - Pf1N1B4_4222 0.67 -1.4 4,679,230 + Pf1N1B4_4222 0.83 -2.2 4,679,231 - -0.4 4,679,234 + Pf1N1B4_4222 0.83 -0.6 4,679,234 + Pf1N1B4_4222 0.83 -0.6 4,679,235 - Pf1N1B4_4222 0.83 -0.4 4,679,235 - Pf1N1B4_4222 0.83 -2.2 4,679,290 + Pf1N1B4_4222 0.90 -1.7 4,679,290 + Pf1N1B4_4222 0.90 -1.0 4,679,291 - Pf1N1B4_4222 0.90 -0.4 4,679,291 - Pf1N1B4_4222 0.90 +1.2 4,679,291 - Pf1N1B4_4222 0.90 -0.2 4,679,335 + -0.0 4,679,336 - +1.4 4,679,345 - +0.2 4,679,347 + +0.3 4,679,347 + -2.2 4,679,347 + -0.2 4,679,347 + +1.3 4,679,348 - +1.2 4,679,348 - -0.0 4,679,348 - -0.2 4,679,353 + -0.0 4,679,377 + -0.0 4,679,377 + +0.6 4,679,501 + Pf1N1B4_4223 0.13 -0.8 4,679,502 - +0.6 4,679,734 + Pf1N1B4_4223 0.39 -0.8 4,679,734 + Pf1N1B4_4223 0.39 +1.2 4,679,807 + Pf1N1B4_4223 0.47 +0.0 4,679,884 - +0.6 4,679,933 + +0.6 4,679,934 - Pf1N1B4_4223 0.61 -1.6 4,679,934 - Pf1N1B4_4223 0.61 -0.6 4,680,137 - Pf1N1B4_4223 0.84 +0.2 4,680,244 - -0.4 4,680,303 + -0.4 4,680,380 - +2.0 4,680,403 - -0.9 4,680,433 + -1.0 4,680,433 + -0.8 4,680,437 + -0.7 4,680,437 + -0.4 4,680,438 - -1.0 4,680,542 + +0.4 4,680,793 + +1.0 4,680,946 + -0.0 4,680,946 + -0.1 4,680,946 + +1.0 4,680,947 - -0.4 4,680,982 + +0.6 4,680,982 + -2.2 4,681,083 + +0.2 4,681,083 + Pf1N1B4_4225 0.14 -0.7
Or see this region's nucleotide sequence