Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4166 and Pf1N1B4_4167 are separated by 122 nucleotides Pf1N1B4_4167 and Pf1N1B4_4168 overlap by 1 nucleotides Pf1N1B4_4168 and Pf1N1B4_4169 are separated by 1 nucleotides
Pf1N1B4_4166 - Transcriptional regulator, LysR family, at 4,616,918 to 4,617,823
_4166
Pf1N1B4_4167 - hypothetical protein, at 4,617,946 to 4,618,296
_4167
Pf1N1B4_4168 - putative membrane protein, at 4,618,296 to 4,618,808
_4168
Pf1N1B4_4169 - Hydrolases of the alpha/beta superfamily, at 4,618,810 to 4,619,559
_4169
Position (kb)
4618
4619 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4617.416 kb on + strand, within Pf1N1B4_4166 at 4617.416 kb on + strand, within Pf1N1B4_4166 at 4617.432 kb on - strand, within Pf1N1B4_4166 at 4617.432 kb on - strand, within Pf1N1B4_4166 at 4617.521 kb on + strand, within Pf1N1B4_4166 at 4618.421 kb on + strand, within Pf1N1B4_4168 at 4618.421 kb on + strand, within Pf1N1B4_4168 at 4618.421 kb on + strand, within Pf1N1B4_4168 at 4618.422 kb on - strand at 4618.422 kb on - strand at 4618.422 kb on - strand at 4618.422 kb on - strand, within Pf1N1B4_4168 at 4618.493 kb on + strand, within Pf1N1B4_4168 at 4618.800 kb on + strand at 4618.800 kb on + strand at 4618.838 kb on + strand at 4618.919 kb on + strand, within Pf1N1B4_4169 at 4618.919 kb on + strand, within Pf1N1B4_4169 at 4618.919 kb on + strand at 4618.919 kb on + strand, within Pf1N1B4_4169 at 4618.920 kb on - strand, within Pf1N1B4_4169 at 4619.182 kb on - strand, within Pf1N1B4_4169 at 4619.198 kb on + strand, within Pf1N1B4_4169 at 4619.291 kb on + strand at 4619.291 kb on + strand, within Pf1N1B4_4169 at 4619.291 kb on + strand, within Pf1N1B4_4169 at 4619.291 kb on + strand, within Pf1N1B4_4169 at 4619.291 kb on + strand, within Pf1N1B4_4169 at 4619.291 kb on + strand, within Pf1N1B4_4169 at 4619.480 kb on + strand, within Pf1N1B4_4169 at 4619.480 kb on + strand, within Pf1N1B4_4169 at 4619.480 kb on + strand, within Pf1N1B4_4169 at 4619.480 kb on + strand, within Pf1N1B4_4169 at 4619.480 kb on + strand, within Pf1N1B4_4169 at 4619.480 kb on + strand, within Pf1N1B4_4169 at 4619.528 kb on + strand at 4619.580 kb on + strand at 4619.581 kb on - strand at 4619.624 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,617,416 + Pf1N1B4_4166 0.55 -1.7 4,617,416 + Pf1N1B4_4166 0.55 +0.3 4,617,432 - Pf1N1B4_4166 0.57 -1.2 4,617,432 - Pf1N1B4_4166 0.57 -0.7 4,617,521 + Pf1N1B4_4166 0.67 -0.4 4,618,421 + Pf1N1B4_4168 0.24 -0.4 4,618,421 + Pf1N1B4_4168 0.24 +0.6 4,618,421 + Pf1N1B4_4168 0.24 -0.4 4,618,422 - -1.0 4,618,422 - +2.6 4,618,422 - -0.4 4,618,422 - Pf1N1B4_4168 0.25 -2.2 4,618,493 + Pf1N1B4_4168 0.38 -1.1 4,618,800 + -0.9 4,618,800 + -1.4 4,618,838 + -1.0 4,618,919 + Pf1N1B4_4169 0.15 +1.6 4,618,919 + Pf1N1B4_4169 0.15 -1.4 4,618,919 + +1.6 4,618,919 + Pf1N1B4_4169 0.15 +0.3 4,618,920 - Pf1N1B4_4169 0.15 -0.1 4,619,182 - Pf1N1B4_4169 0.50 +0.3 4,619,198 + Pf1N1B4_4169 0.52 -0.1 4,619,291 + -0.4 4,619,291 + Pf1N1B4_4169 0.64 +2.6 4,619,291 + Pf1N1B4_4169 0.64 -0.4 4,619,291 + Pf1N1B4_4169 0.64 -0.7 4,619,291 + Pf1N1B4_4169 0.64 -1.2 4,619,291 + Pf1N1B4_4169 0.64 +0.0 4,619,480 + Pf1N1B4_4169 0.89 -0.8 4,619,480 + Pf1N1B4_4169 0.89 +0.6 4,619,480 + Pf1N1B4_4169 0.89 +0.2 4,619,480 + Pf1N1B4_4169 0.89 -1.7 4,619,480 + Pf1N1B4_4169 0.89 +3.2 4,619,480 + Pf1N1B4_4169 0.89 -1.2 4,619,528 + +0.6 4,619,580 + -1.7 4,619,581 - +1.2 4,619,624 + -0.0
Or see this region's nucleotide sequence