Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4039 and Pf1N1B4_4040 are separated by 72 nucleotides Pf1N1B4_4040 and Pf1N1B4_4041 are separated by 10 nucleotides Pf1N1B4_4041 and Pf1N1B4_4042 are separated by 649 nucleotides
Pf1N1B4_4039 - NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3), at 4,490,237 to 4,492,021
_4039
Pf1N1B4_4040 - NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3), at 4,492,094 to 4,492,768
_4040
Pf1N1B4_4041 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 4,492,779 to 4,493,192
_4041
Pf1N1B4_4042 - Isocitrate lyase (EC 4.1.3.1) (from data) , at 4,493,842 to 4,495,167
_4042
Position (kb)
4492
4493
4494 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4491.816 kb on + strand, within Pf1N1B4_4039 at 4491.817 kb on - strand, within Pf1N1B4_4039 at 4491.817 kb on - strand, within Pf1N1B4_4039 at 4491.817 kb on - strand, within Pf1N1B4_4039 at 4491.828 kb on + strand, within Pf1N1B4_4039 at 4491.829 kb on - strand, within Pf1N1B4_4039 at 4491.987 kb on + strand at 4491.988 kb on - strand at 4492.000 kb on - strand at 4492.000 kb on - strand at 4492.025 kb on + strand at 4492.025 kb on + strand at 4492.026 kb on - strand at 4492.026 kb on - strand at 4492.026 kb on - strand at 4492.026 kb on - strand at 4492.048 kb on - strand at 4492.048 kb on - strand at 4492.048 kb on - strand at 4492.048 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.079 kb on - strand at 4492.133 kb on - strand at 4492.296 kb on + strand, within Pf1N1B4_4040 at 4492.297 kb on - strand, within Pf1N1B4_4040 at 4492.350 kb on + strand, within Pf1N1B4_4040 at 4492.351 kb on - strand, within Pf1N1B4_4040 at 4492.396 kb on + strand at 4492.563 kb on + strand, within Pf1N1B4_4040 at 4492.585 kb on + strand, within Pf1N1B4_4040 at 4492.586 kb on - strand, within Pf1N1B4_4040 at 4492.761 kb on + strand at 4492.762 kb on - strand at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.853 kb on - strand, within Pf1N1B4_4041 at 4492.879 kb on - strand, within Pf1N1B4_4041 at 4492.994 kb on - strand, within Pf1N1B4_4041 at 4492.994 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.035 kb on - strand, within Pf1N1B4_4041 at 4493.284 kb on + strand at 4493.326 kb on - strand at 4493.329 kb on + strand at 4493.330 kb on - strand at 4493.345 kb on + strand at 4493.406 kb on + strand at 4493.406 kb on + strand at 4493.406 kb on + strand at 4493.425 kb on - strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.433 kb on + strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.434 kb on - strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.513 kb on + strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.514 kb on - strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.515 kb on + strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.516 kb on - strand at 4493.551 kb on + strand at 4493.780 kb on + strand at 4493.781 kb on - strand at 4493.781 kb on - strand at 4493.825 kb on + strand at 4493.930 kb on + strand at 4494.111 kb on - strand, within Pf1N1B4_4042
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,491,816 + Pf1N1B4_4039 0.88 -2.5 4,491,817 - Pf1N1B4_4039 0.89 -1.2 4,491,817 - Pf1N1B4_4039 0.89 -0.1 4,491,817 - Pf1N1B4_4039 0.89 +0.1 4,491,828 + Pf1N1B4_4039 0.89 -2.0 4,491,829 - Pf1N1B4_4039 0.89 +0.7 4,491,987 + +0.8 4,491,988 - -0.3 4,492,000 - +0.7 4,492,000 - -1.0 4,492,025 + -1.7 4,492,025 + -1.7 4,492,026 - -1.1 4,492,026 - -0.1 4,492,026 - -2.7 4,492,026 - -0.3 4,492,048 - -0.4 4,492,048 - +1.7 4,492,048 - -0.8 4,492,048 - -0.3 4,492,079 - -1.1 4,492,079 - +0.1 4,492,079 - +1.2 4,492,079 - -0.6 4,492,079 - -0.9 4,492,133 - -2.3 4,492,296 + Pf1N1B4_4040 0.30 +1.1 4,492,297 - Pf1N1B4_4040 0.30 -2.1 4,492,350 + Pf1N1B4_4040 0.38 +0.1 4,492,351 - Pf1N1B4_4040 0.38 -0.9 4,492,396 + -0.3 4,492,563 + Pf1N1B4_4040 0.69 -1.1 4,492,585 + Pf1N1B4_4040 0.73 -0.7 4,492,586 - Pf1N1B4_4040 0.73 -1.1 4,492,761 + +0.1 4,492,762 - -0.7 4,492,853 - Pf1N1B4_4041 0.18 -2.0 4,492,853 - Pf1N1B4_4041 0.18 -0.8 4,492,853 - Pf1N1B4_4041 0.18 +0.3 4,492,879 - Pf1N1B4_4041 0.24 -0.3 4,492,994 - Pf1N1B4_4041 0.52 -0.3 4,492,994 - Pf1N1B4_4041 0.52 -0.3 4,493,035 - Pf1N1B4_4041 0.62 -1.9 4,493,035 - Pf1N1B4_4041 0.62 +0.5 4,493,035 - Pf1N1B4_4041 0.62 -0.3 4,493,284 + -0.3 4,493,326 - +0.7 4,493,329 + -0.8 4,493,330 - -0.7 4,493,345 + -0.3 4,493,406 + -0.3 4,493,406 + -0.7 4,493,406 + -2.5 4,493,425 - -2.1 4,493,433 + +1.7 4,493,433 + -2.1 4,493,433 + -1.1 4,493,433 + -0.6 4,493,433 + +1.3 4,493,433 + +1.7 4,493,433 + -1.4 4,493,433 + +0.7 4,493,433 + -0.3 4,493,433 + -0.9 4,493,433 + -2.1 4,493,434 - -0.7 4,493,434 - -0.3 4,493,434 - +0.7 4,493,434 - -0.7 4,493,434 - +0.3 4,493,513 + +0.7 4,493,513 + -0.7 4,493,513 + +0.7 4,493,513 + -2.1 4,493,514 - -0.3 4,493,514 - -0.7 4,493,514 - +0.1 4,493,514 - +1.7 4,493,514 - -0.7 4,493,515 + +1.9 4,493,515 + -1.7 4,493,515 + -1.7 4,493,515 + -1.3 4,493,515 + +0.7 4,493,516 - -0.2 4,493,516 - -0.1 4,493,516 - +0.7 4,493,516 - +0.3 4,493,551 + -2.8 4,493,780 + -0.8 4,493,781 - -0.3 4,493,781 - -1.1 4,493,825 + -0.7 4,493,930 + +0.1 4,494,111 - Pf1N1B4_4042 0.20 -1.3
Or see this region's nucleotide sequence