Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3987 and Pf1N1B4_3988 are separated by 328 nucleotides Pf1N1B4_3988 and Pf1N1B4_3989 are separated by 38 nucleotides
Pf1N1B4_3987 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4), at 4,429,380 to 4,430,543
_3987
Pf1N1B4_3988 - Acetoacetyl-CoA synthetase (EC 6.2.1.16) / Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 4,430,872 to 4,432,578
_3988
Pf1N1B4_3989 - small molecule metabolism, at 4,432,617 to 4,433,678
_3989
Position (kb)
4430
4431
4432
4433 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4430.023 kb on + strand, within Pf1N1B4_3987 at 4430.023 kb on + strand, within Pf1N1B4_3987 at 4430.024 kb on - strand at 4430.032 kb on + strand, within Pf1N1B4_3987 at 4430.032 kb on + strand, within Pf1N1B4_3987 at 4430.033 kb on - strand, within Pf1N1B4_3987 at 4430.082 kb on + strand, within Pf1N1B4_3987 at 4430.083 kb on - strand, within Pf1N1B4_3987 at 4430.086 kb on + strand, within Pf1N1B4_3987 at 4430.102 kb on + strand, within Pf1N1B4_3987 at 4430.200 kb on + strand, within Pf1N1B4_3987 at 4430.201 kb on - strand, within Pf1N1B4_3987 at 4430.201 kb on - strand, within Pf1N1B4_3987 at 4430.353 kb on + strand, within Pf1N1B4_3987 at 4430.537 kb on - strand at 4430.611 kb on + strand at 4430.611 kb on + strand at 4430.671 kb on - strand at 4430.671 kb on - strand at 4430.671 kb on - strand at 4430.753 kb on + strand at 4430.753 kb on + strand at 4430.753 kb on + strand at 4430.757 kb on + strand at 4430.757 kb on + strand at 4430.757 kb on + strand at 4430.757 kb on + strand at 4430.757 kb on + strand at 4430.758 kb on - strand at 4430.758 kb on - strand at 4430.758 kb on - strand at 4430.768 kb on - strand at 4430.847 kb on + strand at 4430.847 kb on + strand at 4430.848 kb on - strand at 4430.852 kb on + strand at 4430.905 kb on + strand at 4430.906 kb on - strand at 4431.220 kb on + strand, within Pf1N1B4_3988 at 4431.220 kb on + strand, within Pf1N1B4_3988 at 4431.221 kb on - strand, within Pf1N1B4_3988 at 4431.221 kb on - strand, within Pf1N1B4_3988 at 4431.221 kb on - strand, within Pf1N1B4_3988 at 4431.511 kb on + strand, within Pf1N1B4_3988 at 4431.512 kb on - strand, within Pf1N1B4_3988 at 4431.559 kb on + strand, within Pf1N1B4_3988 at 4431.559 kb on + strand, within Pf1N1B4_3988 at 4431.560 kb on - strand at 4431.560 kb on - strand, within Pf1N1B4_3988 at 4431.560 kb on - strand, within Pf1N1B4_3988 at 4431.560 kb on - strand, within Pf1N1B4_3988 at 4431.643 kb on + strand, within Pf1N1B4_3988 at 4431.644 kb on - strand, within Pf1N1B4_3988 at 4431.697 kb on - strand, within Pf1N1B4_3988 at 4431.697 kb on - strand at 4431.707 kb on - strand, within Pf1N1B4_3988 at 4432.109 kb on - strand, within Pf1N1B4_3988 at 4432.216 kb on + strand, within Pf1N1B4_3988 at 4432.269 kb on + strand, within Pf1N1B4_3988 at 4432.269 kb on + strand, within Pf1N1B4_3988 at 4432.273 kb on + strand, within Pf1N1B4_3988 at 4432.466 kb on - strand at 4432.618 kb on + strand at 4432.618 kb on + strand at 4432.619 kb on - strand at 4432.619 kb on - strand at 4432.742 kb on + strand, within Pf1N1B4_3989 at 4432.742 kb on + strand, within Pf1N1B4_3989 at 4432.743 kb on - strand, within Pf1N1B4_3989 at 4432.743 kb on - strand, within Pf1N1B4_3989 at 4432.743 kb on - strand, within Pf1N1B4_3989 at 4432.834 kb on + strand, within Pf1N1B4_3989 at 4432.835 kb on - strand at 4432.860 kb on - strand, within Pf1N1B4_3989 at 4433.059 kb on + strand, within Pf1N1B4_3989 at 4433.060 kb on - strand, within Pf1N1B4_3989 at 4433.060 kb on - strand, within Pf1N1B4_3989 at 4433.104 kb on + strand, within Pf1N1B4_3989 at 4433.104 kb on + strand, within Pf1N1B4_3989 at 4433.105 kb on - strand, within Pf1N1B4_3989 at 4433.160 kb on + strand, within Pf1N1B4_3989 at 4433.161 kb on - strand, within Pf1N1B4_3989 at 4433.161 kb on - strand, within Pf1N1B4_3989 at 4433.163 kb on + strand, within Pf1N1B4_3989 at 4433.247 kb on + strand, within Pf1N1B4_3989 at 4433.247 kb on + strand, within Pf1N1B4_3989 at 4433.247 kb on + strand at 4433.277 kb on + strand, within Pf1N1B4_3989 at 4433.277 kb on + strand, within Pf1N1B4_3989 at 4433.455 kb on + strand, within Pf1N1B4_3989 at 4433.455 kb on + strand, within Pf1N1B4_3989 at 4433.455 kb on + strand, within Pf1N1B4_3989 at 4433.552 kb on + strand, within Pf1N1B4_3989
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,430,023 + Pf1N1B4_3987 0.55 +1.0 4,430,023 + Pf1N1B4_3987 0.55 +1.3 4,430,024 - -0.3 4,430,032 + Pf1N1B4_3987 0.56 +0.3 4,430,032 + Pf1N1B4_3987 0.56 +0.1 4,430,033 - Pf1N1B4_3987 0.56 -0.9 4,430,082 + Pf1N1B4_3987 0.60 +1.7 4,430,083 - Pf1N1B4_3987 0.60 -1.5 4,430,086 + Pf1N1B4_3987 0.61 -0.9 4,430,102 + Pf1N1B4_3987 0.62 +0.4 4,430,200 + Pf1N1B4_3987 0.70 -0.9 4,430,201 - Pf1N1B4_3987 0.71 +0.2 4,430,201 - Pf1N1B4_3987 0.71 -0.0 4,430,353 + Pf1N1B4_3987 0.84 -0.0 4,430,537 - -0.3 4,430,611 + -1.5 4,430,611 + +2.5 4,430,671 - -0.5 4,430,671 - -1.6 4,430,671 - +1.1 4,430,753 + +1.0 4,430,753 + -0.9 4,430,753 + -0.3 4,430,757 + -0.3 4,430,757 + -0.3 4,430,757 + -0.8 4,430,757 + +0.1 4,430,757 + +0.3 4,430,758 - -0.3 4,430,758 - -0.5 4,430,758 - +0.3 4,430,768 - -1.6 4,430,847 + +0.1 4,430,847 + -1.3 4,430,848 - +0.7 4,430,852 + +0.0 4,430,905 + -1.0 4,430,906 - -0.2 4,431,220 + Pf1N1B4_3988 0.20 -1.0 4,431,220 + Pf1N1B4_3988 0.20 -0.3 4,431,221 - Pf1N1B4_3988 0.20 -1.9 4,431,221 - Pf1N1B4_3988 0.20 -2.1 4,431,221 - Pf1N1B4_3988 0.20 -1.3 4,431,511 + Pf1N1B4_3988 0.37 +0.0 4,431,512 - Pf1N1B4_3988 0.37 -1.6 4,431,559 + Pf1N1B4_3988 0.40 -1.5 4,431,559 + Pf1N1B4_3988 0.40 -1.6 4,431,560 - +1.3 4,431,560 - Pf1N1B4_3988 0.40 +0.5 4,431,560 - Pf1N1B4_3988 0.40 -0.3 4,431,560 - Pf1N1B4_3988 0.40 -0.3 4,431,643 + Pf1N1B4_3988 0.45 +0.3 4,431,644 - Pf1N1B4_3988 0.45 +0.5 4,431,697 - Pf1N1B4_3988 0.48 -0.3 4,431,697 - +0.1 4,431,707 - Pf1N1B4_3988 0.49 -0.3 4,432,109 - Pf1N1B4_3988 0.72 -0.9 4,432,216 + Pf1N1B4_3988 0.79 -0.3 4,432,269 + Pf1N1B4_3988 0.82 +0.3 4,432,269 + Pf1N1B4_3988 0.82 -0.6 4,432,273 + Pf1N1B4_3988 0.82 -1.0 4,432,466 - -0.0 4,432,618 + -0.6 4,432,618 + +0.9 4,432,619 - -0.0 4,432,619 - -2.1 4,432,742 + Pf1N1B4_3989 0.12 -1.3 4,432,742 + Pf1N1B4_3989 0.12 +1.2 4,432,743 - Pf1N1B4_3989 0.12 -2.3 4,432,743 - Pf1N1B4_3989 0.12 -1.9 4,432,743 - Pf1N1B4_3989 0.12 -1.2 4,432,834 + Pf1N1B4_3989 0.20 +0.7 4,432,835 - +0.7 4,432,860 - Pf1N1B4_3989 0.23 -1.5 4,433,059 + Pf1N1B4_3989 0.42 +0.4 4,433,060 - Pf1N1B4_3989 0.42 -0.3 4,433,060 - Pf1N1B4_3989 0.42 -0.6 4,433,104 + Pf1N1B4_3989 0.46 +0.1 4,433,104 + Pf1N1B4_3989 0.46 -0.4 4,433,105 - Pf1N1B4_3989 0.46 -0.3 4,433,160 + Pf1N1B4_3989 0.51 -0.3 4,433,161 - Pf1N1B4_3989 0.51 -1.1 4,433,161 - Pf1N1B4_3989 0.51 +0.2 4,433,163 + Pf1N1B4_3989 0.51 +0.1 4,433,247 + Pf1N1B4_3989 0.59 -0.3 4,433,247 + Pf1N1B4_3989 0.59 +2.3 4,433,247 + +0.1 4,433,277 + Pf1N1B4_3989 0.62 +1.7 4,433,277 + Pf1N1B4_3989 0.62 +0.6 4,433,455 + Pf1N1B4_3989 0.79 +1.0 4,433,455 + Pf1N1B4_3989 0.79 -1.2 4,433,455 + Pf1N1B4_3989 0.79 -0.1 4,433,552 + Pf1N1B4_3989 0.88 +0.3
Or see this region's nucleotide sequence