Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3865 and Pf1N1B4_3866 are separated by 137 nucleotides Pf1N1B4_3866 and Pf1N1B4_3867 are separated by 79 nucleotides Pf1N1B4_3867 and Pf1N1B4_3868 are separated by 93 nucleotides
Pf1N1B4_3865 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 4,294,629 to 4,295,777
_3865
Pf1N1B4_3866 - Adenine phosphoribosyltransferase (EC 2.4.2.7), at 4,295,915 to 4,296,463
_3866
Pf1N1B4_3867 - Fumarate and nitrate reduction regulatory protein, at 4,296,543 to 4,297,277
_3867
Pf1N1B4_3868 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 4,297,371 to 4,298,753
_3868
Position (kb)
4295
4296
4297 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4295.535 kb on + strand, within Pf1N1B4_3865 at 4295.664 kb on + strand at 4295.665 kb on - strand at 4295.665 kb on - strand at 4295.665 kb on - strand at 4295.665 kb on - strand at 4295.665 kb on - strand at 4295.665 kb on - strand at 4295.666 kb on + strand at 4295.666 kb on + strand at 4295.666 kb on + strand at 4295.667 kb on - strand at 4295.667 kb on - strand at 4295.667 kb on - strand at 4295.667 kb on - strand at 4295.753 kb on + strand at 4295.753 kb on + strand at 4295.760 kb on - strand at 4295.843 kb on + strand at 4295.843 kb on + strand at 4295.843 kb on + strand at 4295.843 kb on + strand at 4295.843 kb on + strand at 4295.844 kb on - strand at 4295.844 kb on - strand at 4295.844 kb on - strand at 4295.854 kb on + strand at 4295.854 kb on + strand at 4295.855 kb on - strand at 4295.855 kb on - strand at 4295.855 kb on - strand at 4295.855 kb on - strand at 4295.904 kb on - strand at 4295.916 kb on + strand at 4295.916 kb on + strand at 4295.916 kb on + strand at 4295.916 kb on + strand at 4295.916 kb on + strand at 4295.917 kb on - strand at 4295.917 kb on - strand at 4295.917 kb on - strand at 4295.917 kb on - strand at 4295.917 kb on - strand at 4295.921 kb on - strand at 4295.921 kb on - strand at 4295.954 kb on - strand at 4295.954 kb on - strand at 4295.954 kb on - strand at 4295.954 kb on - strand at 4296.221 kb on + strand, within Pf1N1B4_3866 at 4296.222 kb on - strand, within Pf1N1B4_3866 at 4296.499 kb on + strand at 4296.499 kb on + strand at 4296.596 kb on + strand at 4296.597 kb on - strand at 4296.600 kb on + strand at 4296.600 kb on + strand at 4296.601 kb on - strand at 4296.685 kb on + strand, within Pf1N1B4_3867 at 4296.686 kb on - strand, within Pf1N1B4_3867 at 4297.307 kb on - strand at 4297.373 kb on - strand at 4297.373 kb on - strand at 4297.373 kb on - strand at 4297.373 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,295,535 + Pf1N1B4_3865 0.79 +0.4 4,295,664 + +0.5 4,295,665 - +0.4 4,295,665 - +1.8 4,295,665 - +0.8 4,295,665 - +1.4 4,295,665 - -1.2 4,295,665 - +2.4 4,295,666 + +0.1 4,295,666 + +1.1 4,295,666 + +2.6 4,295,667 - -0.6 4,295,667 - +1.1 4,295,667 - -0.2 4,295,667 - -1.5 4,295,753 + -0.4 4,295,753 + -1.5 4,295,760 - -0.4 4,295,843 + -0.5 4,295,843 + +0.2 4,295,843 + +0.1 4,295,843 + -0.5 4,295,843 + -1.0 4,295,844 - -1.5 4,295,844 - -1.3 4,295,844 - -0.0 4,295,854 + -0.2 4,295,854 + -1.4 4,295,855 - +1.4 4,295,855 - -0.2 4,295,855 - -0.2 4,295,855 - -0.8 4,295,904 - +0.6 4,295,916 + -0.0 4,295,916 + +0.8 4,295,916 + +0.0 4,295,916 + +0.2 4,295,916 + -0.1 4,295,917 - +1.8 4,295,917 - +1.8 4,295,917 - +0.0 4,295,917 - -0.8 4,295,917 - -0.2 4,295,921 - -1.8 4,295,921 - -1.8 4,295,954 - -0.8 4,295,954 - -1.6 4,295,954 - +1.4 4,295,954 - -0.6 4,296,221 + Pf1N1B4_3866 0.56 -0.2 4,296,222 - Pf1N1B4_3866 0.56 -1.4 4,296,499 + +1.4 4,296,499 + +0.6 4,296,596 + -1.1 4,296,597 - +0.0 4,296,600 + -1.2 4,296,600 + -0.8 4,296,601 - -0.8 4,296,685 + Pf1N1B4_3867 0.19 +0.9 4,296,686 - Pf1N1B4_3867 0.19 -2.2 4,297,307 - +1.1 4,297,373 - +0.5 4,297,373 - +1.4 4,297,373 - -1.0 4,297,373 - +0.1
Or see this region's nucleotide sequence