Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3843 and Pf1N1B4_3844 are separated by 530 nucleotides Pf1N1B4_3844 and Pf1N1B4_3845 are separated by 142 nucleotides
Pf1N1B4_3843 - Ribosome modulation factor, at 4,260,931 to 4,261,146
_3843
Pf1N1B4_3844 - 23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-), at 4,261,677 to 4,263,947
_3844
Pf1N1B4_3845 - CHASE domain/PAS domain protein, at 4,264,090 to 4,266,480
_3845
Position (kb)
4261
4262
4263
4264 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4260.726 kb on + strand at 4260.726 kb on + strand at 4260.809 kb on + strand at 4260.809 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.811 kb on + strand at 4260.812 kb on - strand at 4260.884 kb on + strand at 4260.885 kb on - strand at 4260.885 kb on - strand at 4260.930 kb on - strand at 4260.985 kb on - strand, within Pf1N1B4_3843 at 4261.053 kb on + strand, within Pf1N1B4_3843 at 4261.053 kb on + strand, within Pf1N1B4_3843 at 4261.054 kb on - strand, within Pf1N1B4_3843 at 4261.054 kb on - strand, within Pf1N1B4_3843 at 4261.054 kb on - strand, within Pf1N1B4_3843 at 4261.092 kb on - strand, within Pf1N1B4_3843 at 4261.134 kb on + strand at 4261.134 kb on + strand at 4261.134 kb on + strand at 4261.338 kb on + strand at 4261.365 kb on + strand at 4261.374 kb on - strand at 4261.374 kb on - strand at 4261.475 kb on + strand at 4261.538 kb on + strand at 4261.538 kb on + strand at 4261.538 kb on + strand at 4261.538 kb on + strand at 4261.539 kb on - strand at 4261.539 kb on - strand at 4261.539 kb on - strand at 4261.539 kb on - strand at 4261.539 kb on - strand at 4261.539 kb on - strand at 4261.548 kb on + strand at 4261.654 kb on + strand at 4261.654 kb on + strand at 4261.654 kb on + strand at 4261.673 kb on + strand at 4262.156 kb on + strand, within Pf1N1B4_3844 at 4262.667 kb on + strand, within Pf1N1B4_3844 at 4262.667 kb on + strand, within Pf1N1B4_3844 at 4262.952 kb on + strand, within Pf1N1B4_3844 at 4262.952 kb on + strand, within Pf1N1B4_3844 at 4262.952 kb on + strand, within Pf1N1B4_3844 at 4262.953 kb on - strand, within Pf1N1B4_3844 at 4262.953 kb on - strand, within Pf1N1B4_3844 at 4262.953 kb on - strand, within Pf1N1B4_3844 at 4262.953 kb on - strand, within Pf1N1B4_3844 at 4263.068 kb on + strand, within Pf1N1B4_3844 at 4263.108 kb on + strand, within Pf1N1B4_3844 at 4263.129 kb on + strand, within Pf1N1B4_3844 at 4263.150 kb on + strand, within Pf1N1B4_3844 at 4263.151 kb on - strand, within Pf1N1B4_3844 at 4263.396 kb on + strand, within Pf1N1B4_3844 at 4263.396 kb on + strand, within Pf1N1B4_3844 at 4263.483 kb on + strand, within Pf1N1B4_3844 at 4263.490 kb on - strand, within Pf1N1B4_3844 at 4263.801 kb on + strand at 4263.801 kb on + strand at 4263.801 kb on + strand at 4264.028 kb on + strand at 4264.028 kb on + strand at 4264.033 kb on - strand at 4264.035 kb on - strand at 4264.085 kb on + strand at 4264.085 kb on + strand at 4264.085 kb on + strand at 4264.085 kb on + strand at 4264.086 kb on - strand at 4264.086 kb on - strand at 4264.086 kb on - strand at 4264.089 kb on + strand at 4264.089 kb on + strand at 4264.089 kb on + strand at 4264.089 kb on + strand at 4264.089 kb on + strand at 4264.090 kb on - strand at 4264.090 kb on - strand at 4264.090 kb on - strand at 4264.090 kb on - strand at 4264.090 kb on - strand at 4264.090 kb on - strand at 4264.411 kb on + strand, within Pf1N1B4_3845 at 4264.412 kb on - strand, within Pf1N1B4_3845 at 4264.412 kb on - strand, within Pf1N1B4_3845 at 4264.549 kb on + strand, within Pf1N1B4_3845 at 4264.550 kb on - strand, within Pf1N1B4_3845 at 4264.550 kb on - strand, within Pf1N1B4_3845 at 4264.550 kb on - strand, within Pf1N1B4_3845 at 4264.564 kb on + strand, within Pf1N1B4_3845 at 4264.731 kb on - strand, within Pf1N1B4_3845 at 4264.860 kb on + strand, within Pf1N1B4_3845 at 4264.895 kb on + strand, within Pf1N1B4_3845 at 4264.895 kb on + strand, within Pf1N1B4_3845 at 4264.895 kb on + strand, within Pf1N1B4_3845 at 4264.895 kb on + strand, within Pf1N1B4_3845 at 4264.896 kb on - strand, within Pf1N1B4_3845 at 4264.896 kb on - strand, within Pf1N1B4_3845 at 4264.900 kb on + strand, within Pf1N1B4_3845 at 4264.900 kb on + strand, within Pf1N1B4_3845
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,260,726 + +0.9 4,260,726 + -1.7 4,260,809 + +0.5 4,260,809 + +0.4 4,260,811 + -0.2 4,260,811 + -0.5 4,260,811 + -0.4 4,260,811 + -2.2 4,260,811 + -0.2 4,260,811 + +0.4 4,260,811 + +0.2 4,260,811 + -1.8 4,260,811 + -0.2 4,260,812 - -0.8 4,260,884 + +0.4 4,260,885 - -0.2 4,260,885 - +2.1 4,260,930 - -0.1 4,260,985 - Pf1N1B4_3843 0.25 -0.2 4,261,053 + Pf1N1B4_3843 0.56 +0.2 4,261,053 + Pf1N1B4_3843 0.56 +0.2 4,261,054 - Pf1N1B4_3843 0.57 -0.8 4,261,054 - Pf1N1B4_3843 0.57 -0.1 4,261,054 - Pf1N1B4_3843 0.57 -0.7 4,261,092 - Pf1N1B4_3843 0.75 +0.5 4,261,134 + +0.8 4,261,134 + -1.4 4,261,134 + -1.2 4,261,338 + -0.1 4,261,365 + -0.2 4,261,374 - -0.5 4,261,374 - -0.8 4,261,475 + -0.6 4,261,538 + -0.2 4,261,538 + -1.4 4,261,538 + +1.8 4,261,538 + +0.8 4,261,539 - -0.4 4,261,539 - +1.8 4,261,539 - -1.7 4,261,539 - -0.8 4,261,539 - +0.4 4,261,539 - -1.0 4,261,548 + -0.0 4,261,654 + -0.0 4,261,654 + +2.4 4,261,654 + +0.0 4,261,673 + -0.8 4,262,156 + Pf1N1B4_3844 0.21 -1.5 4,262,667 + Pf1N1B4_3844 0.44 -2.0 4,262,667 + Pf1N1B4_3844 0.44 -0.7 4,262,952 + Pf1N1B4_3844 0.56 -0.2 4,262,952 + Pf1N1B4_3844 0.56 -1.2 4,262,952 + Pf1N1B4_3844 0.56 -0.2 4,262,953 - Pf1N1B4_3844 0.56 +0.8 4,262,953 - Pf1N1B4_3844 0.56 -1.4 4,262,953 - Pf1N1B4_3844 0.56 +1.8 4,262,953 - Pf1N1B4_3844 0.56 -0.2 4,263,068 + Pf1N1B4_3844 0.61 -2.5 4,263,108 + Pf1N1B4_3844 0.63 -1.5 4,263,129 + Pf1N1B4_3844 0.64 -1.5 4,263,150 + Pf1N1B4_3844 0.65 +0.3 4,263,151 - Pf1N1B4_3844 0.65 -0.6 4,263,396 + Pf1N1B4_3844 0.76 +2.8 4,263,396 + Pf1N1B4_3844 0.76 -0.4 4,263,483 + Pf1N1B4_3844 0.80 -0.8 4,263,490 - Pf1N1B4_3844 0.80 -2.0 4,263,801 + -1.2 4,263,801 + +0.4 4,263,801 + -0.2 4,264,028 + -0.2 4,264,028 + -1.0 4,264,033 - -0.5 4,264,035 - +0.2 4,264,085 + -0.2 4,264,085 + +0.8 4,264,085 + +0.2 4,264,085 + -0.0 4,264,086 - -0.4 4,264,086 - +0.5 4,264,086 - +0.4 4,264,089 + -2.2 4,264,089 + -0.8 4,264,089 + +0.8 4,264,089 + +0.2 4,264,089 + +0.2 4,264,090 - +0.8 4,264,090 - +0.8 4,264,090 - +0.3 4,264,090 - +0.6 4,264,090 - +0.3 4,264,090 - +0.0 4,264,411 + Pf1N1B4_3845 0.13 +0.3 4,264,412 - Pf1N1B4_3845 0.13 +0.5 4,264,412 - Pf1N1B4_3845 0.13 +0.4 4,264,549 + Pf1N1B4_3845 0.19 +0.8 4,264,550 - Pf1N1B4_3845 0.19 +0.3 4,264,550 - Pf1N1B4_3845 0.19 +0.6 4,264,550 - Pf1N1B4_3845 0.19 -0.8 4,264,564 + Pf1N1B4_3845 0.20 +0.0 4,264,731 - Pf1N1B4_3845 0.27 +1.1 4,264,860 + Pf1N1B4_3845 0.32 +0.8 4,264,895 + Pf1N1B4_3845 0.34 +4.0 4,264,895 + Pf1N1B4_3845 0.34 -1.7 4,264,895 + Pf1N1B4_3845 0.34 -0.1 4,264,895 + Pf1N1B4_3845 0.34 -0.5 4,264,896 - Pf1N1B4_3845 0.34 -1.0 4,264,896 - Pf1N1B4_3845 0.34 -0.4 4,264,900 + Pf1N1B4_3845 0.34 -0.2 4,264,900 + Pf1N1B4_3845 0.34 -0.5
Or see this region's nucleotide sequence