Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_380 and Pf1N1B4_381 are separated by 293 nucleotides Pf1N1B4_381 and Pf1N1B4_382 are separated by 60 nucleotides
Pf1N1B4_380 - Oxidoreductase, short chain dehydrogenase/reductase family, at 449,774 to 450,589
_380
Pf1N1B4_381 - hypothetical protein, at 450,883 to 451,035
_381
Pf1N1B4_382 - Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily, at 451,096 to 453,189
_382
Position (kb)
450
451
452 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 449.928 kb on + strand, within Pf1N1B4_380 at 449.934 kb on + strand, within Pf1N1B4_380 at 449.934 kb on + strand, within Pf1N1B4_380 at 449.934 kb on + strand, within Pf1N1B4_380 at 449.935 kb on - strand, within Pf1N1B4_380 at 449.935 kb on - strand, within Pf1N1B4_380 at 449.935 kb on - strand at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.141 kb on + strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.142 kb on - strand, within Pf1N1B4_380 at 450.641 kb on - strand at 450.727 kb on - strand at 450.735 kb on + strand at 450.752 kb on + strand at 450.753 kb on - strand at 450.757 kb on + strand at 450.803 kb on - strand at 450.810 kb on + strand at 450.810 kb on + strand at 450.816 kb on + strand at 450.817 kb on - strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.819 kb on + strand at 450.820 kb on - strand at 450.820 kb on - strand at 450.820 kb on - strand at 450.846 kb on + strand at 450.897 kb on + strand at 450.910 kb on - strand at 450.923 kb on + strand, within Pf1N1B4_381 at 450.923 kb on + strand, within Pf1N1B4_381 at 450.923 kb on + strand, within Pf1N1B4_381 at 450.923 kb on + strand, within Pf1N1B4_381 at 450.923 kb on + strand, within Pf1N1B4_381 at 450.924 kb on - strand, within Pf1N1B4_381 at 451.004 kb on + strand, within Pf1N1B4_381 at 451.020 kb on - strand at 451.031 kb on + strand at 451.032 kb on - strand at 451.032 kb on - strand at 451.033 kb on + strand at 451.033 kb on + strand at 451.033 kb on + strand at 451.083 kb on - strand at 451.250 kb on + strand at 451.250 kb on + strand at 451.251 kb on - strand at 451.251 kb on - strand at 451.251 kb on - strand at 451.461 kb on + strand, within Pf1N1B4_382 at 451.462 kb on - strand, within Pf1N1B4_382 at 451.608 kb on - strand, within Pf1N1B4_382 at 451.608 kb on - strand, within Pf1N1B4_382 at 451.608 kb on - strand, within Pf1N1B4_382 at 451.608 kb on - strand, within Pf1N1B4_382 at 451.772 kb on + strand, within Pf1N1B4_382 at 451.772 kb on + strand, within Pf1N1B4_382 at 451.772 kb on + strand, within Pf1N1B4_382 at 451.772 kb on + strand, within Pf1N1B4_382 at 451.773 kb on - strand, within Pf1N1B4_382 at 451.773 kb on - strand, within Pf1N1B4_382 at 451.890 kb on + strand, within Pf1N1B4_382 at 451.890 kb on + strand, within Pf1N1B4_382 at 451.891 kb on - strand, within Pf1N1B4_382 at 451.891 kb on - strand at 451.964 kb on + strand, within Pf1N1B4_382 at 451.964 kb on + strand, within Pf1N1B4_382 at 451.964 kb on + strand, within Pf1N1B4_382 at 451.965 kb on - strand, within Pf1N1B4_382 at 451.965 kb on - strand, within Pf1N1B4_382 at 451.965 kb on - strand, within Pf1N1B4_382 at 451.965 kb on - strand, within Pf1N1B4_382 at 451.965 kb on - strand, within Pf1N1B4_382 at 451.970 kb on + strand, within Pf1N1B4_382 at 451.970 kb on + strand at 451.970 kb on + strand, within Pf1N1B4_382 at 451.970 kb on + strand, within Pf1N1B4_382 at 451.970 kb on + strand, within Pf1N1B4_382 at 451.970 kb on + strand, within Pf1N1B4_382 at 451.970 kb on + strand, within Pf1N1B4_382 at 451.970 kb on - strand, within Pf1N1B4_382 at 451.971 kb on - strand, within Pf1N1B4_382 at 451.971 kb on - strand, within Pf1N1B4_382 at 451.971 kb on - strand, within Pf1N1B4_382 at 451.971 kb on - strand, within Pf1N1B4_382
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 449,928 + Pf1N1B4_380 0.19 -0.2 449,934 + Pf1N1B4_380 0.20 -2.3 449,934 + Pf1N1B4_380 0.20 -1.3 449,934 + Pf1N1B4_380 0.20 +0.8 449,935 - Pf1N1B4_380 0.20 -0.4 449,935 - Pf1N1B4_380 0.20 +0.5 449,935 - +2.4 450,141 + Pf1N1B4_380 0.45 -0.6 450,141 + Pf1N1B4_380 0.45 +1.4 450,141 + Pf1N1B4_380 0.45 -0.2 450,141 + Pf1N1B4_380 0.45 -0.2 450,142 - Pf1N1B4_380 0.45 -0.7 450,142 - Pf1N1B4_380 0.45 +0.1 450,142 - -0.2 450,142 - Pf1N1B4_380 0.45 -1.5 450,142 - Pf1N1B4_380 0.45 +1.8 450,142 - Pf1N1B4_380 0.45 -0.1 450,641 - +0.6 450,727 - +0.8 450,735 + +1.6 450,752 + +1.4 450,753 - -0.2 450,757 + +0.5 450,803 - -1.5 450,810 + -1.4 450,810 + +0.8 450,816 + -0.2 450,817 - -0.5 450,819 + -1.5 450,819 + +0.8 450,819 + -0.3 450,819 + +0.3 450,819 + -1.2 450,819 + -1.5 450,820 - -2.0 450,820 - +1.4 450,820 - -1.7 450,846 + -1.0 450,897 + -2.0 450,910 - -0.2 450,923 + Pf1N1B4_381 0.26 -2.2 450,923 + Pf1N1B4_381 0.26 -1.0 450,923 + Pf1N1B4_381 0.26 +0.5 450,923 + Pf1N1B4_381 0.26 +0.8 450,923 + Pf1N1B4_381 0.26 -1.2 450,924 - Pf1N1B4_381 0.27 +0.1 451,004 + Pf1N1B4_381 0.79 -0.2 451,020 - -1.5 451,031 + -1.0 451,032 - -0.7 451,032 - +0.5 451,033 + +1.8 451,033 + +0.5 451,033 + -0.2 451,083 - +0.8 451,250 + +1.3 451,250 + -2.5 451,251 - +0.8 451,251 - +0.8 451,251 - -1.0 451,461 + Pf1N1B4_382 0.17 +0.8 451,462 - Pf1N1B4_382 0.17 -0.7 451,608 - Pf1N1B4_382 0.24 -1.5 451,608 - Pf1N1B4_382 0.24 +1.8 451,608 - Pf1N1B4_382 0.24 +1.2 451,608 - Pf1N1B4_382 0.24 +0.7 451,772 + Pf1N1B4_382 0.32 -0.7 451,772 + Pf1N1B4_382 0.32 -0.7 451,772 + Pf1N1B4_382 0.32 +1.2 451,772 + Pf1N1B4_382 0.32 -2.2 451,773 - Pf1N1B4_382 0.32 -0.2 451,773 - Pf1N1B4_382 0.32 +0.3 451,890 + Pf1N1B4_382 0.38 +1.0 451,890 + Pf1N1B4_382 0.38 +0.8 451,891 - Pf1N1B4_382 0.38 -1.5 451,891 - +2.8 451,964 + Pf1N1B4_382 0.41 +0.5 451,964 + Pf1N1B4_382 0.41 -1.0 451,964 + Pf1N1B4_382 0.41 +0.3 451,965 - Pf1N1B4_382 0.41 +2.7 451,965 - Pf1N1B4_382 0.41 +0.1 451,965 - Pf1N1B4_382 0.41 +0.8 451,965 - Pf1N1B4_382 0.41 -0.2 451,965 - Pf1N1B4_382 0.41 +0.1 451,970 + Pf1N1B4_382 0.42 +0.7 451,970 + -0.2 451,970 + Pf1N1B4_382 0.42 +0.8 451,970 + Pf1N1B4_382 0.42 -1.3 451,970 + Pf1N1B4_382 0.42 +3.2 451,970 + Pf1N1B4_382 0.42 +1.2 451,970 + Pf1N1B4_382 0.42 -0.2 451,970 - Pf1N1B4_382 0.42 -0.6 451,971 - Pf1N1B4_382 0.42 -0.3 451,971 - Pf1N1B4_382 0.42 -0.9 451,971 - Pf1N1B4_382 0.42 -1.0 451,971 - Pf1N1B4_382 0.42 +0.7
Or see this region's nucleotide sequence