Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3707 and Pf1N1B4_3708 overlap by 4 nucleotides Pf1N1B4_3708 and Pf1N1B4_3709 overlap by 4 nucleotides Pf1N1B4_3709 and Pf1N1B4_3710 overlap by 8 nucleotides Pf1N1B4_3710 and Pf1N1B4_3711 overlap by 4 nucleotides
Pf1N1B4_3707 - Cobyrinic acid A,C-diamide synthase, at 4,115,664 to 4,117,046
_3707
Pf1N1B4_3708 - Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family, at 4,117,043 to 4,117,693
_3708
Pf1N1B4_3709 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 4,117,690 to 4,118,598
_3709
Pf1N1B4_3710 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 4,118,591 to 4,119,583
_3710
Pf1N1B4_3711 - Cobyric acid synthase (EC 6.3.5.10), at 4,119,580 to 4,121,073
_3711
Position (kb)
4117
4118
4119 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4116.789 kb on + strand, within Pf1N1B4_3707 at 4116.789 kb on + strand, within Pf1N1B4_3707 at 4116.789 kb on + strand at 4116.789 kb on + strand, within Pf1N1B4_3707 at 4116.789 kb on + strand, within Pf1N1B4_3707 at 4116.789 kb on + strand, within Pf1N1B4_3707 at 4116.789 kb on + strand, within Pf1N1B4_3707 at 4116.900 kb on + strand, within Pf1N1B4_3707 at 4116.900 kb on + strand, within Pf1N1B4_3707 at 4116.900 kb on + strand at 4116.901 kb on - strand, within Pf1N1B4_3707 at 4116.901 kb on - strand, within Pf1N1B4_3707 at 4116.912 kb on + strand at 4116.912 kb on + strand at 4117.149 kb on + strand, within Pf1N1B4_3708 at 4117.149 kb on + strand, within Pf1N1B4_3708 at 4117.359 kb on + strand, within Pf1N1B4_3708 at 4117.360 kb on - strand, within Pf1N1B4_3708 at 4117.620 kb on + strand, within Pf1N1B4_3708 at 4117.664 kb on + strand at 4117.664 kb on + strand at 4117.665 kb on - strand at 4117.673 kb on + strand at 4117.674 kb on - strand at 4117.909 kb on + strand, within Pf1N1B4_3709 at 4117.909 kb on + strand, within Pf1N1B4_3709 at 4117.942 kb on + strand at 4117.942 kb on + strand at 4117.942 kb on + strand, within Pf1N1B4_3709 at 4118.284 kb on + strand at 4118.284 kb on + strand, within Pf1N1B4_3709 at 4118.284 kb on + strand, within Pf1N1B4_3709 at 4118.284 kb on + strand, within Pf1N1B4_3709 at 4118.285 kb on - strand, within Pf1N1B4_3709 at 4118.285 kb on - strand, within Pf1N1B4_3709 at 4118.315 kb on - strand, within Pf1N1B4_3709 at 4118.452 kb on + strand, within Pf1N1B4_3709 at 4118.452 kb on + strand, within Pf1N1B4_3709 at 4118.452 kb on + strand, within Pf1N1B4_3709 at 4118.591 kb on - strand at 4118.774 kb on + strand, within Pf1N1B4_3710 at 4118.774 kb on + strand, within Pf1N1B4_3710 at 4118.877 kb on - strand, within Pf1N1B4_3710 at 4118.912 kb on + strand, within Pf1N1B4_3710 at 4118.912 kb on + strand, within Pf1N1B4_3710 at 4118.913 kb on - strand, within Pf1N1B4_3710 at 4118.913 kb on - strand, within Pf1N1B4_3710 at 4118.913 kb on - strand, within Pf1N1B4_3710 at 4119.331 kb on + strand, within Pf1N1B4_3710 at 4119.375 kb on - strand, within Pf1N1B4_3710 at 4119.563 kb on + strand at 4119.564 kb on - strand at 4119.564 kb on - strand at 4119.585 kb on + strand at 4119.585 kb on + strand at 4119.586 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,116,789 + Pf1N1B4_3707 0.81 -0.6 4,116,789 + Pf1N1B4_3707 0.81 -0.9 4,116,789 + -0.4 4,116,789 + Pf1N1B4_3707 0.81 -0.2 4,116,789 + Pf1N1B4_3707 0.81 -0.5 4,116,789 + Pf1N1B4_3707 0.81 +0.0 4,116,789 + Pf1N1B4_3707 0.81 -1.2 4,116,900 + Pf1N1B4_3707 0.89 +1.1 4,116,900 + Pf1N1B4_3707 0.89 -0.3 4,116,900 + -0.2 4,116,901 - Pf1N1B4_3707 0.89 -2.2 4,116,901 - Pf1N1B4_3707 0.89 +2.8 4,116,912 + -1.2 4,116,912 + -2.3 4,117,149 + Pf1N1B4_3708 0.16 -0.2 4,117,149 + Pf1N1B4_3708 0.16 -0.8 4,117,359 + Pf1N1B4_3708 0.49 -1.1 4,117,360 - Pf1N1B4_3708 0.49 -0.2 4,117,620 + Pf1N1B4_3708 0.89 +0.8 4,117,664 + -0.8 4,117,664 + -0.2 4,117,665 - +0.8 4,117,673 + +0.8 4,117,674 - -1.0 4,117,909 + Pf1N1B4_3709 0.24 +0.8 4,117,909 + Pf1N1B4_3709 0.24 +0.2 4,117,942 + +1.6 4,117,942 + -0.8 4,117,942 + Pf1N1B4_3709 0.28 -1.8 4,118,284 + +0.8 4,118,284 + Pf1N1B4_3709 0.65 +0.5 4,118,284 + Pf1N1B4_3709 0.65 -0.6 4,118,284 + Pf1N1B4_3709 0.65 -1.2 4,118,285 - Pf1N1B4_3709 0.65 -1.6 4,118,285 - Pf1N1B4_3709 0.65 -2.0 4,118,315 - Pf1N1B4_3709 0.69 +1.8 4,118,452 + Pf1N1B4_3709 0.84 -0.6 4,118,452 + Pf1N1B4_3709 0.84 +0.8 4,118,452 + Pf1N1B4_3709 0.84 -1.1 4,118,591 - +2.4 4,118,774 + Pf1N1B4_3710 0.18 +0.2 4,118,774 + Pf1N1B4_3710 0.18 -0.2 4,118,877 - Pf1N1B4_3710 0.29 +0.9 4,118,912 + Pf1N1B4_3710 0.32 -0.9 4,118,912 + Pf1N1B4_3710 0.32 -0.6 4,118,913 - Pf1N1B4_3710 0.32 -0.6 4,118,913 - Pf1N1B4_3710 0.32 -0.0 4,118,913 - Pf1N1B4_3710 0.32 +0.2 4,119,331 + Pf1N1B4_3710 0.75 +0.1 4,119,375 - Pf1N1B4_3710 0.79 -0.4 4,119,563 + +0.5 4,119,564 - -0.2 4,119,564 - -0.8 4,119,585 + +0.8 4,119,585 + -0.5 4,119,586 - +0.8
Or see this region's nucleotide sequence