Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3584 and Pf1N1B4_3585 are separated by 30 nucleotides Pf1N1B4_3585 and Pf1N1B4_3586 overlap by 4 nucleotides
Pf1N1B4_3584 - O-antigen biosynthesis protein, at 3,994,479 to 3,997,415
_3584
Pf1N1B4_3585 - UDP-N-acetylglucosamine 4,6-dehydratase (EC 4.2.1.-), at 3,997,446 to 3,998,447
_3585
Pf1N1B4_3586 - DegT/DnrJ/EryC1/StrS aminotransferase, at 3,998,444 to 3,999,607
_3586
Position (kb)
3997
3998
3999 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3996.843 kb on + strand, within Pf1N1B4_3584 at 3996.844 kb on - strand, within Pf1N1B4_3584 at 3997.051 kb on - strand, within Pf1N1B4_3584 at 3997.170 kb on + strand at 3997.332 kb on + strand at 3997.367 kb on + strand at 3997.414 kb on - strand at 3997.431 kb on - strand at 3997.431 kb on - strand at 3997.575 kb on + strand at 3997.615 kb on - strand, within Pf1N1B4_3585 at 3997.719 kb on + strand, within Pf1N1B4_3585 at 3997.719 kb on + strand, within Pf1N1B4_3585 at 3997.720 kb on - strand, within Pf1N1B4_3585 at 3998.274 kb on + strand, within Pf1N1B4_3585 at 3998.303 kb on + strand, within Pf1N1B4_3585 at 3998.303 kb on + strand, within Pf1N1B4_3585 at 3998.768 kb on + strand, within Pf1N1B4_3586 at 3998.860 kb on + strand, within Pf1N1B4_3586 at 3998.860 kb on + strand, within Pf1N1B4_3586 at 3998.900 kb on - strand, within Pf1N1B4_3586 at 3998.939 kb on + strand, within Pf1N1B4_3586 at 3998.939 kb on + strand, within Pf1N1B4_3586 at 3998.939 kb on + strand, within Pf1N1B4_3586 at 3998.940 kb on - strand, within Pf1N1B4_3586 at 3998.940 kb on - strand, within Pf1N1B4_3586 at 3999.140 kb on + strand, within Pf1N1B4_3586 at 3999.144 kb on - strand, within Pf1N1B4_3586 at 3999.167 kb on - strand, within Pf1N1B4_3586 at 3999.281 kb on + strand, within Pf1N1B4_3586 at 3999.281 kb on + strand, within Pf1N1B4_3586 at 3999.338 kb on + strand, within Pf1N1B4_3586 at 3999.338 kb on + strand at 3999.338 kb on + strand, within Pf1N1B4_3586 at 3999.339 kb on - strand, within Pf1N1B4_3586 at 3999.339 kb on - strand, within Pf1N1B4_3586 at 3999.339 kb on - strand, within Pf1N1B4_3586 at 3999.431 kb on + strand at 3999.431 kb on + strand, within Pf1N1B4_3586 at 3999.431 kb on + strand, within Pf1N1B4_3586 at 3999.432 kb on - strand, within Pf1N1B4_3586 at 3999.432 kb on - strand, within Pf1N1B4_3586 at 3999.432 kb on - strand, within Pf1N1B4_3586 at 3999.433 kb on + strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586 at 3999.434 kb on - strand, within Pf1N1B4_3586
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,996,843 + Pf1N1B4_3584 0.80 +0.3 3,996,844 - Pf1N1B4_3584 0.81 +0.9 3,997,051 - Pf1N1B4_3584 0.88 -0.7 3,997,170 + -0.2 3,997,332 + +0.3 3,997,367 + +0.3 3,997,414 - -0.7 3,997,431 - +0.1 3,997,431 - +2.5 3,997,575 + +0.9 3,997,615 - Pf1N1B4_3585 0.17 -0.6 3,997,719 + Pf1N1B4_3585 0.27 -1.4 3,997,719 + Pf1N1B4_3585 0.27 -0.5 3,997,720 - Pf1N1B4_3585 0.27 -2.3 3,998,274 + Pf1N1B4_3585 0.83 -0.7 3,998,303 + Pf1N1B4_3585 0.86 +0.4 3,998,303 + Pf1N1B4_3585 0.86 -0.4 3,998,768 + Pf1N1B4_3586 0.28 +0.6 3,998,860 + Pf1N1B4_3586 0.36 -0.1 3,998,860 + Pf1N1B4_3586 0.36 +1.4 3,998,900 - Pf1N1B4_3586 0.39 -0.3 3,998,939 + Pf1N1B4_3586 0.43 +1.5 3,998,939 + Pf1N1B4_3586 0.43 +0.5 3,998,939 + Pf1N1B4_3586 0.43 -0.5 3,998,940 - Pf1N1B4_3586 0.43 +0.5 3,998,940 - Pf1N1B4_3586 0.43 +0.3 3,999,140 + Pf1N1B4_3586 0.60 -1.1 3,999,144 - Pf1N1B4_3586 0.60 -0.0 3,999,167 - Pf1N1B4_3586 0.62 -0.5 3,999,281 + Pf1N1B4_3586 0.72 -0.7 3,999,281 + Pf1N1B4_3586 0.72 +0.7 3,999,338 + Pf1N1B4_3586 0.77 +0.1 3,999,338 + +1.9 3,999,338 + Pf1N1B4_3586 0.77 -1.1 3,999,339 - Pf1N1B4_3586 0.77 +1.0 3,999,339 - Pf1N1B4_3586 0.77 -0.1 3,999,339 - Pf1N1B4_3586 0.77 -0.7 3,999,431 + +0.9 3,999,431 + Pf1N1B4_3586 0.85 -0.7 3,999,431 + Pf1N1B4_3586 0.85 +0.6 3,999,432 - Pf1N1B4_3586 0.85 -1.6 3,999,432 - Pf1N1B4_3586 0.85 -0.6 3,999,432 - Pf1N1B4_3586 0.85 +1.3 3,999,433 + Pf1N1B4_3586 0.85 +0.1 3,999,434 - Pf1N1B4_3586 0.85 +2.2 3,999,434 - Pf1N1B4_3586 0.85 +0.9 3,999,434 - Pf1N1B4_3586 0.85 +0.9 3,999,434 - Pf1N1B4_3586 0.85 +0.9 3,999,434 - Pf1N1B4_3586 0.85 +0.3 3,999,434 - Pf1N1B4_3586 0.85 -0.7 3,999,434 - Pf1N1B4_3586 0.85 +0.9
Or see this region's nucleotide sequence