Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3582 and Pf1N1B4_3583 are separated by 16 nucleotides Pf1N1B4_3583 and Pf1N1B4_3584 are separated by 51 nucleotides Pf1N1B4_3584 and Pf1N1B4_3585 are separated by 30 nucleotides
Pf1N1B4_3582 - C-methyltransferase, at 3,992,603 to 3,993,673
_3582
Pf1N1B4_3583 - Cephalosporin hydroxylase, at 3,993,690 to 3,994,427
_3583
Pf1N1B4_3584 - O-antigen biosynthesis protein, at 3,994,479 to 3,997,415
_3584
Pf1N1B4_3585 - UDP-N-acetylglucosamine 4,6-dehydratase (EC 4.2.1.-), at 3,997,446 to 3,998,447
_3585
Position (kb)
3994
3995
3996
3997
3998 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3993.599 kb on + strand at 3993.599 kb on + strand at 3993.600 kb on - strand at 3993.635 kb on + strand at 3993.636 kb on - strand at 3993.687 kb on - strand at 3993.817 kb on - strand at 3993.817 kb on - strand, within Pf1N1B4_3583 at 3993.817 kb on - strand, within Pf1N1B4_3583 at 3993.823 kb on - strand at 3993.957 kb on + strand, within Pf1N1B4_3583 at 3994.064 kb on + strand, within Pf1N1B4_3583 at 3994.065 kb on - strand, within Pf1N1B4_3583 at 3994.065 kb on - strand, within Pf1N1B4_3583 at 3994.200 kb on + strand, within Pf1N1B4_3583 at 3994.228 kb on - strand, within Pf1N1B4_3583 at 3994.228 kb on - strand, within Pf1N1B4_3583 at 3994.329 kb on + strand, within Pf1N1B4_3583 at 3994.330 kb on - strand, within Pf1N1B4_3583 at 3994.588 kb on - strand at 3994.590 kb on + strand at 3994.590 kb on + strand at 3994.591 kb on - strand at 3994.591 kb on - strand at 3994.591 kb on - strand at 3994.599 kb on + strand at 3994.600 kb on - strand at 3994.701 kb on + strand at 3994.702 kb on - strand at 3994.702 kb on - strand at 3994.755 kb on + strand at 3994.755 kb on + strand at 3994.755 kb on + strand at 3994.755 kb on + strand at 3994.839 kb on + strand, within Pf1N1B4_3584 at 3994.844 kb on + strand, within Pf1N1B4_3584 at 3994.844 kb on + strand, within Pf1N1B4_3584 at 3994.844 kb on + strand, within Pf1N1B4_3584 at 3994.844 kb on + strand, within Pf1N1B4_3584 at 3994.845 kb on - strand, within Pf1N1B4_3584 at 3994.845 kb on - strand, within Pf1N1B4_3584 at 3994.869 kb on + strand, within Pf1N1B4_3584 at 3994.869 kb on + strand, within Pf1N1B4_3584 at 3994.870 kb on - strand, within Pf1N1B4_3584 at 3994.870 kb on - strand, within Pf1N1B4_3584 at 3994.887 kb on + strand, within Pf1N1B4_3584 at 3994.887 kb on + strand, within Pf1N1B4_3584 at 3994.888 kb on - strand, within Pf1N1B4_3584 at 3994.888 kb on - strand, within Pf1N1B4_3584 at 3994.888 kb on - strand, within Pf1N1B4_3584 at 3994.888 kb on - strand, within Pf1N1B4_3584 at 3994.888 kb on - strand, within Pf1N1B4_3584 at 3994.896 kb on + strand, within Pf1N1B4_3584 at 3994.897 kb on - strand at 3994.899 kb on + strand, within Pf1N1B4_3584 at 3994.918 kb on + strand, within Pf1N1B4_3584 at 3994.965 kb on + strand at 3994.966 kb on - strand, within Pf1N1B4_3584 at 3994.977 kb on + strand, within Pf1N1B4_3584 at 3994.977 kb on + strand, within Pf1N1B4_3584 at 3994.978 kb on - strand, within Pf1N1B4_3584 at 3994.978 kb on - strand, within Pf1N1B4_3584 at 3994.978 kb on - strand at 3994.978 kb on - strand, within Pf1N1B4_3584 at 3994.992 kb on + strand, within Pf1N1B4_3584 at 3994.993 kb on - strand, within Pf1N1B4_3584 at 3995.028 kb on + strand, within Pf1N1B4_3584 at 3995.082 kb on + strand, within Pf1N1B4_3584 at 3995.083 kb on - strand, within Pf1N1B4_3584 at 3995.083 kb on - strand, within Pf1N1B4_3584 at 3995.083 kb on - strand, within Pf1N1B4_3584 at 3995.085 kb on + strand, within Pf1N1B4_3584 at 3995.085 kb on + strand, within Pf1N1B4_3584 at 3995.085 kb on + strand, within Pf1N1B4_3584 at 3995.086 kb on - strand, within Pf1N1B4_3584 at 3995.086 kb on - strand, within Pf1N1B4_3584 at 3995.086 kb on - strand, within Pf1N1B4_3584 at 3995.127 kb on + strand, within Pf1N1B4_3584 at 3995.127 kb on + strand, within Pf1N1B4_3584 at 3995.127 kb on + strand, within Pf1N1B4_3584 at 3995.127 kb on + strand, within Pf1N1B4_3584 at 3995.128 kb on - strand, within Pf1N1B4_3584 at 3995.427 kb on + strand, within Pf1N1B4_3584 at 3995.427 kb on + strand, within Pf1N1B4_3584 at 3995.427 kb on + strand, within Pf1N1B4_3584 at 3995.427 kb on + strand, within Pf1N1B4_3584 at 3995.427 kb on + strand, within Pf1N1B4_3584 at 3995.427 kb on + strand, within Pf1N1B4_3584 at 3995.428 kb on - strand, within Pf1N1B4_3584 at 3995.428 kb on - strand at 3995.428 kb on - strand, within Pf1N1B4_3584 at 3995.428 kb on - strand, within Pf1N1B4_3584 at 3995.428 kb on - strand, within Pf1N1B4_3584 at 3995.445 kb on + strand, within Pf1N1B4_3584 at 3995.555 kb on + strand, within Pf1N1B4_3584 at 3995.555 kb on + strand, within Pf1N1B4_3584 at 3995.555 kb on + strand at 3995.556 kb on - strand, within Pf1N1B4_3584 at 3995.556 kb on - strand, within Pf1N1B4_3584 at 3995.556 kb on - strand, within Pf1N1B4_3584 at 3995.556 kb on - strand, within Pf1N1B4_3584 at 3995.556 kb on - strand, within Pf1N1B4_3584 at 3995.556 kb on - strand, within Pf1N1B4_3584 at 3995.572 kb on - strand, within Pf1N1B4_3584 at 3996.054 kb on + strand, within Pf1N1B4_3584 at 3996.054 kb on + strand at 3996.054 kb on + strand at 3996.054 kb on + strand, within Pf1N1B4_3584 at 3996.843 kb on + strand, within Pf1N1B4_3584 at 3996.844 kb on - strand, within Pf1N1B4_3584 at 3997.051 kb on - strand, within Pf1N1B4_3584 at 3997.170 kb on + strand at 3997.332 kb on + strand at 3997.367 kb on + strand at 3997.414 kb on - strand at 3997.431 kb on - strand at 3997.431 kb on - strand at 3997.575 kb on + strand at 3997.615 kb on - strand, within Pf1N1B4_3585 at 3997.719 kb on + strand, within Pf1N1B4_3585 at 3997.719 kb on + strand, within Pf1N1B4_3585 at 3997.720 kb on - strand, within Pf1N1B4_3585 at 3998.274 kb on + strand, within Pf1N1B4_3585 at 3998.303 kb on + strand, within Pf1N1B4_3585 at 3998.303 kb on + strand, within Pf1N1B4_3585
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,993,599 + -0.9 3,993,599 + +0.1 3,993,600 - -0.1 3,993,635 + +1.9 3,993,636 - -0.3 3,993,687 - +0.9 3,993,817 - -1.1 3,993,817 - Pf1N1B4_3583 0.17 -1.5 3,993,817 - Pf1N1B4_3583 0.17 -0.9 3,993,823 - +0.9 3,993,957 + Pf1N1B4_3583 0.36 -0.5 3,994,064 + Pf1N1B4_3583 0.51 +0.9 3,994,065 - Pf1N1B4_3583 0.51 -0.5 3,994,065 - Pf1N1B4_3583 0.51 -0.7 3,994,200 + Pf1N1B4_3583 0.69 +0.9 3,994,228 - Pf1N1B4_3583 0.73 +1.3 3,994,228 - Pf1N1B4_3583 0.73 +1.4 3,994,329 + Pf1N1B4_3583 0.87 -0.2 3,994,330 - Pf1N1B4_3583 0.87 -1.0 3,994,588 - +0.9 3,994,590 + -0.6 3,994,590 + -0.0 3,994,591 - -0.8 3,994,591 - +0.9 3,994,591 - -0.1 3,994,599 + -0.7 3,994,600 - -1.7 3,994,701 + +1.6 3,994,702 - +0.1 3,994,702 - -1.1 3,994,755 + -0.7 3,994,755 + -1.1 3,994,755 + +1.5 3,994,755 + +0.1 3,994,839 + Pf1N1B4_3584 0.12 +0.3 3,994,844 + Pf1N1B4_3584 0.12 -0.1 3,994,844 + Pf1N1B4_3584 0.12 +1.3 3,994,844 + Pf1N1B4_3584 0.12 -1.1 3,994,844 + Pf1N1B4_3584 0.12 +0.3 3,994,845 - Pf1N1B4_3584 0.12 -2.1 3,994,845 - Pf1N1B4_3584 0.12 +0.2 3,994,869 + Pf1N1B4_3584 0.13 +0.6 3,994,869 + Pf1N1B4_3584 0.13 +0.9 3,994,870 - Pf1N1B4_3584 0.13 -0.3 3,994,870 - Pf1N1B4_3584 0.13 -0.7 3,994,887 + Pf1N1B4_3584 0.14 -0.5 3,994,887 + Pf1N1B4_3584 0.14 -1.7 3,994,888 - Pf1N1B4_3584 0.14 -0.1 3,994,888 - Pf1N1B4_3584 0.14 +0.0 3,994,888 - Pf1N1B4_3584 0.14 -0.1 3,994,888 - Pf1N1B4_3584 0.14 -0.8 3,994,888 - Pf1N1B4_3584 0.14 +0.3 3,994,896 + Pf1N1B4_3584 0.14 -1.1 3,994,897 - +0.9 3,994,899 + Pf1N1B4_3584 0.14 -0.7 3,994,918 + Pf1N1B4_3584 0.15 +0.9 3,994,965 + +1.5 3,994,966 - Pf1N1B4_3584 0.17 -0.1 3,994,977 + Pf1N1B4_3584 0.17 +1.6 3,994,977 + Pf1N1B4_3584 0.17 +0.5 3,994,978 - Pf1N1B4_3584 0.17 +1.6 3,994,978 - Pf1N1B4_3584 0.17 -0.7 3,994,978 - +0.9 3,994,978 - Pf1N1B4_3584 0.17 -1.1 3,994,992 + Pf1N1B4_3584 0.17 -1.1 3,994,993 - Pf1N1B4_3584 0.18 +1.0 3,995,028 + Pf1N1B4_3584 0.19 +0.2 3,995,082 + Pf1N1B4_3584 0.21 -0.1 3,995,083 - Pf1N1B4_3584 0.21 +1.5 3,995,083 - Pf1N1B4_3584 0.21 +0.7 3,995,083 - Pf1N1B4_3584 0.21 +0.6 3,995,085 + Pf1N1B4_3584 0.21 +0.1 3,995,085 + Pf1N1B4_3584 0.21 -0.5 3,995,085 + Pf1N1B4_3584 0.21 -0.9 3,995,086 - Pf1N1B4_3584 0.21 +0.9 3,995,086 - Pf1N1B4_3584 0.21 +1.2 3,995,086 - Pf1N1B4_3584 0.21 +0.5 3,995,127 + Pf1N1B4_3584 0.22 -0.3 3,995,127 + Pf1N1B4_3584 0.22 +0.0 3,995,127 + Pf1N1B4_3584 0.22 -0.1 3,995,127 + Pf1N1B4_3584 0.22 +1.9 3,995,128 - Pf1N1B4_3584 0.22 +0.6 3,995,427 + Pf1N1B4_3584 0.32 +1.9 3,995,427 + Pf1N1B4_3584 0.32 +0.6 3,995,427 + Pf1N1B4_3584 0.32 -0.1 3,995,427 + Pf1N1B4_3584 0.32 -0.7 3,995,427 + Pf1N1B4_3584 0.32 -0.4 3,995,427 + Pf1N1B4_3584 0.32 -0.0 3,995,428 - Pf1N1B4_3584 0.32 -1.7 3,995,428 - -1.7 3,995,428 - Pf1N1B4_3584 0.32 -0.4 3,995,428 - Pf1N1B4_3584 0.32 -1.4 3,995,428 - Pf1N1B4_3584 0.32 -0.5 3,995,445 + Pf1N1B4_3584 0.33 -0.7 3,995,555 + Pf1N1B4_3584 0.37 -0.7 3,995,555 + Pf1N1B4_3584 0.37 +2.9 3,995,555 + -1.1 3,995,556 - Pf1N1B4_3584 0.37 -0.3 3,995,556 - Pf1N1B4_3584 0.37 -1.7 3,995,556 - Pf1N1B4_3584 0.37 +1.3 3,995,556 - Pf1N1B4_3584 0.37 -0.4 3,995,556 - Pf1N1B4_3584 0.37 -0.1 3,995,556 - Pf1N1B4_3584 0.37 +0.5 3,995,572 - Pf1N1B4_3584 0.37 -0.8 3,996,054 + Pf1N1B4_3584 0.54 +0.9 3,996,054 + -0.1 3,996,054 + -1.4 3,996,054 + Pf1N1B4_3584 0.54 -0.2 3,996,843 + Pf1N1B4_3584 0.80 +0.3 3,996,844 - Pf1N1B4_3584 0.81 +0.9 3,997,051 - Pf1N1B4_3584 0.88 -0.7 3,997,170 + -0.2 3,997,332 + +0.3 3,997,367 + +0.3 3,997,414 - -0.7 3,997,431 - +0.1 3,997,431 - +2.5 3,997,575 + +0.9 3,997,615 - Pf1N1B4_3585 0.17 -0.6 3,997,719 + Pf1N1B4_3585 0.27 -1.4 3,997,719 + Pf1N1B4_3585 0.27 -0.5 3,997,720 - Pf1N1B4_3585 0.27 -2.3 3,998,274 + Pf1N1B4_3585 0.83 -0.7 3,998,303 + Pf1N1B4_3585 0.86 +0.4 3,998,303 + Pf1N1B4_3585 0.86 -0.4
Or see this region's nucleotide sequence