Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3141 and Pf1N1B4_3142 are separated by 80 nucleotides Pf1N1B4_3142 and Pf1N1B4_3143 are separated by 83 nucleotides Pf1N1B4_3143 and Pf1N1B4_3144 are separated by 53 nucleotides Pf1N1B4_3144 and Pf1N1B4_3145 are separated by 43 nucleotides
Pf1N1B4_3141 - FIG00959768: hypothetical protein, at 3,524,567 to 3,525,085
_3141
Pf1N1B4_3142 - C-terminal domain of CinA type S; Protein Implicated in DNA repair function with RecA and MutS, at 3,525,166 to 3,525,666
_3142
Pf1N1B4_3143 - RecA protein, at 3,525,750 to 3,526,802
_3143
Pf1N1B4_3144 - Regulatory protein RecX, at 3,526,856 to 3,527,278
_3144
Pf1N1B4_3145 - Decarboxylase family protein, at 3,527,322 to 3,528,440
_3145
Position (kb)
3525
3526
3527 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3524.818 kb on + strand, within Pf1N1B4_3141 at 3524.819 kb on - strand, within Pf1N1B4_3141 at 3525.005 kb on - strand at 3525.005 kb on - strand at 3525.062 kb on + strand at 3525.062 kb on + strand at 3525.062 kb on + strand at 3525.062 kb on + strand at 3525.063 kb on - strand at 3525.063 kb on - strand at 3525.063 kb on - strand at 3525.063 kb on - strand at 3525.130 kb on - strand at 3525.174 kb on + strand at 3525.174 kb on + strand at 3525.174 kb on + strand at 3525.316 kb on + strand, within Pf1N1B4_3142 at 3525.317 kb on - strand, within Pf1N1B4_3142 at 3525.325 kb on + strand, within Pf1N1B4_3142 at 3525.325 kb on + strand, within Pf1N1B4_3142 at 3525.325 kb on + strand at 3525.326 kb on - strand, within Pf1N1B4_3142 at 3525.326 kb on - strand, within Pf1N1B4_3142 at 3525.326 kb on - strand, within Pf1N1B4_3142 at 3525.473 kb on + strand, within Pf1N1B4_3142 at 3525.474 kb on - strand, within Pf1N1B4_3142 at 3525.565 kb on + strand, within Pf1N1B4_3142 at 3525.565 kb on + strand, within Pf1N1B4_3142 at 3525.565 kb on + strand, within Pf1N1B4_3142 at 3525.728 kb on + strand at 3525.729 kb on - strand at 3525.735 kb on + strand at 3525.735 kb on + strand at 3525.735 kb on + strand at 3525.735 kb on + strand at 3525.939 kb on + strand, within Pf1N1B4_3143 at 3525.939 kb on + strand, within Pf1N1B4_3143 at 3526.398 kb on + strand, within Pf1N1B4_3143 at 3526.398 kb on + strand, within Pf1N1B4_3143 at 3526.551 kb on + strand, within Pf1N1B4_3143 at 3526.551 kb on + strand, within Pf1N1B4_3143 at 3526.552 kb on - strand, within Pf1N1B4_3143 at 3526.617 kb on + strand, within Pf1N1B4_3143 at 3526.617 kb on + strand, within Pf1N1B4_3143 at 3526.822 kb on - strand at 3527.169 kb on + strand, within Pf1N1B4_3144 at 3527.292 kb on + strand at 3527.293 kb on - strand at 3527.293 kb on - strand at 3527.401 kb on + strand at 3527.401 kb on + strand at 3527.401 kb on + strand at 3527.402 kb on - strand at 3527.409 kb on + strand at 3527.410 kb on - strand at 3527.410 kb on - strand at 3527.410 kb on - strand at 3527.410 kb on - strand at 3527.504 kb on - strand, within Pf1N1B4_3145 at 3527.644 kb on + strand, within Pf1N1B4_3145 at 3527.683 kb on + strand, within Pf1N1B4_3145 at 3527.684 kb on - strand at 3527.684 kb on - strand, within Pf1N1B4_3145 at 3527.796 kb on + strand, within Pf1N1B4_3145 at 3527.796 kb on + strand, within Pf1N1B4_3145
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,524,818 + Pf1N1B4_3141 0.48 +1.0 3,524,819 - Pf1N1B4_3141 0.49 +0.3 3,525,005 - -1.3 3,525,005 - -1.3 3,525,062 + -0.9 3,525,062 + +1.3 3,525,062 + +0.0 3,525,062 + +0.1 3,525,063 - -0.8 3,525,063 - -1.0 3,525,063 - +1.0 3,525,063 - +0.3 3,525,130 - +1.0 3,525,174 + +0.2 3,525,174 + +1.0 3,525,174 + -0.3 3,525,316 + Pf1N1B4_3142 0.30 +0.2 3,525,317 - Pf1N1B4_3142 0.30 +0.0 3,525,325 + Pf1N1B4_3142 0.32 +0.0 3,525,325 + Pf1N1B4_3142 0.32 +0.6 3,525,325 + +2.0 3,525,326 - Pf1N1B4_3142 0.32 +0.2 3,525,326 - Pf1N1B4_3142 0.32 +0.3 3,525,326 - Pf1N1B4_3142 0.32 -0.5 3,525,473 + Pf1N1B4_3142 0.61 -0.8 3,525,474 - Pf1N1B4_3142 0.61 -0.5 3,525,565 + Pf1N1B4_3142 0.80 +0.6 3,525,565 + Pf1N1B4_3142 0.80 +0.3 3,525,565 + Pf1N1B4_3142 0.80 -0.1 3,525,728 + -0.4 3,525,729 - -2.5 3,525,735 + +0.5 3,525,735 + -1.1 3,525,735 + +0.0 3,525,735 + -0.4 3,525,939 + Pf1N1B4_3143 0.18 -0.5 3,525,939 + Pf1N1B4_3143 0.18 -1.5 3,526,398 + Pf1N1B4_3143 0.62 -1.8 3,526,398 + Pf1N1B4_3143 0.62 -1.5 3,526,551 + Pf1N1B4_3143 0.76 -2.2 3,526,551 + Pf1N1B4_3143 0.76 +0.2 3,526,552 - Pf1N1B4_3143 0.76 -2.1 3,526,617 + Pf1N1B4_3143 0.82 -2.7 3,526,617 + Pf1N1B4_3143 0.82 +0.0 3,526,822 - +2.0 3,527,169 + Pf1N1B4_3144 0.74 -1.0 3,527,292 + -0.5 3,527,293 - -1.3 3,527,293 - -1.1 3,527,401 + +0.6 3,527,401 + +0.5 3,527,401 + -0.1 3,527,402 - +1.0 3,527,409 + +0.6 3,527,410 - -1.0 3,527,410 - -0.5 3,527,410 - +0.6 3,527,410 - -0.9 3,527,504 - Pf1N1B4_3145 0.16 +0.7 3,527,644 + Pf1N1B4_3145 0.29 +0.6 3,527,683 + Pf1N1B4_3145 0.32 +0.7 3,527,684 - +2.0 3,527,684 - Pf1N1B4_3145 0.32 -2.3 3,527,796 + Pf1N1B4_3145 0.42 -0.7 3,527,796 + Pf1N1B4_3145 0.42 -1.0
Or see this region's nucleotide sequence