Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2850 and Pf1N1B4_2851 overlap by 24 nucleotides Pf1N1B4_2851 and Pf1N1B4_2852 are separated by 111 nucleotides Pf1N1B4_2852 and Pf1N1B4_2853 are separated by 195 nucleotides
Pf1N1B4_2850 - Glucose-6-phosphate isomerase (EC 5.3.1.9), at 3,197,753 to 3,199,417
_2850
Pf1N1B4_2851 - hypothetical protein, at 3,199,394 to 3,199,537
_2851
Pf1N1B4_2852 - Aspartate 1-decarboxylase (EC 4.1.1.11), at 3,199,649 to 3,200,029
_2852
Pf1N1B4_2853 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 3,200,225 to 3,201,085
_2853
Position (kb)
3199
3200 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3198.436 kb on - strand, within Pf1N1B4_2850 at 3198.513 kb on + strand, within Pf1N1B4_2850 at 3198.513 kb on + strand, within Pf1N1B4_2850 at 3198.513 kb on + strand, within Pf1N1B4_2850 at 3198.513 kb on + strand, within Pf1N1B4_2850 at 3198.513 kb on + strand, within Pf1N1B4_2850 at 3198.513 kb on + strand, within Pf1N1B4_2850 at 3198.514 kb on - strand, within Pf1N1B4_2850 at 3198.595 kb on - strand, within Pf1N1B4_2850 at 3198.696 kb on + strand, within Pf1N1B4_2850 at 3198.867 kb on + strand at 3198.868 kb on - strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.049 kb on + strand, within Pf1N1B4_2850 at 3199.050 kb on - strand, within Pf1N1B4_2850 at 3199.050 kb on - strand, within Pf1N1B4_2850 at 3199.050 kb on - strand, within Pf1N1B4_2850 at 3199.050 kb on - strand, within Pf1N1B4_2850 at 3199.050 kb on - strand, within Pf1N1B4_2850 at 3199.050 kb on - strand, within Pf1N1B4_2850 at 3199.252 kb on - strand at 3199.291 kb on + strand at 3199.291 kb on + strand at 3199.410 kb on + strand at 3199.410 kb on + strand at 3199.410 kb on + strand at 3199.411 kb on - strand at 3199.411 kb on - strand at 3199.436 kb on + strand, within Pf1N1B4_2851 at 3199.437 kb on - strand, within Pf1N1B4_2851 at 3199.437 kb on - strand, within Pf1N1B4_2851 at 3199.461 kb on + strand, within Pf1N1B4_2851 at 3199.530 kb on + strand at 3199.531 kb on - strand at 3199.531 kb on - strand at 3199.531 kb on - strand at 3199.531 kb on - strand at 3199.535 kb on + strand at 3199.535 kb on + strand at 3199.535 kb on + strand at 3199.591 kb on + strand at 3199.592 kb on - strand at 3199.598 kb on + strand at 3199.598 kb on + strand at 3199.598 kb on + strand at 3199.598 kb on + strand at 3199.599 kb on - strand at 3199.599 kb on - strand at 3199.599 kb on - strand at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.710 kb on + strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.711 kb on - strand, within Pf1N1B4_2852 at 3199.752 kb on + strand, within Pf1N1B4_2852 at 3199.857 kb on + strand, within Pf1N1B4_2852 at 3199.857 kb on + strand, within Pf1N1B4_2852 at 3199.890 kb on + strand, within Pf1N1B4_2852 at 3199.890 kb on + strand, within Pf1N1B4_2852 at 3199.890 kb on + strand, within Pf1N1B4_2852 at 3199.890 kb on + strand, within Pf1N1B4_2852 at 3199.890 kb on + strand, within Pf1N1B4_2852 at 3199.890 kb on + strand, within Pf1N1B4_2852 at 3199.890 kb on + strand, within Pf1N1B4_2852 at 3199.891 kb on - strand, within Pf1N1B4_2852 at 3199.891 kb on - strand, within Pf1N1B4_2852 at 3199.891 kb on - strand, within Pf1N1B4_2852 at 3199.908 kb on + strand, within Pf1N1B4_2852 at 3199.908 kb on + strand, within Pf1N1B4_2852 at 3199.909 kb on - strand, within Pf1N1B4_2852 at 3199.909 kb on - strand, within Pf1N1B4_2852 at 3199.965 kb on + strand, within Pf1N1B4_2852 at 3199.965 kb on + strand, within Pf1N1B4_2852 at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.093 kb on + strand at 3200.094 kb on - strand at 3200.094 kb on - strand at 3200.149 kb on + strand at 3200.149 kb on + strand at 3200.152 kb on + strand at 3200.152 kb on + strand at 3200.153 kb on - strand at 3200.219 kb on + strand at 3200.226 kb on + strand at 3200.226 kb on + strand at 3200.362 kb on - strand, within Pf1N1B4_2853 at 3200.441 kb on - strand, within Pf1N1B4_2853 at 3200.472 kb on + strand, within Pf1N1B4_2853
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,198,436 - Pf1N1B4_2850 0.41 -0.5 3,198,513 + Pf1N1B4_2850 0.46 +1.1 3,198,513 + Pf1N1B4_2850 0.46 +0.8 3,198,513 + Pf1N1B4_2850 0.46 +0.1 3,198,513 + Pf1N1B4_2850 0.46 +1.1 3,198,513 + Pf1N1B4_2850 0.46 -0.2 3,198,513 + Pf1N1B4_2850 0.46 +0.7 3,198,514 - Pf1N1B4_2850 0.46 -0.5 3,198,595 - Pf1N1B4_2850 0.51 -0.5 3,198,696 + Pf1N1B4_2850 0.57 +0.8 3,198,867 + -0.7 3,198,868 - Pf1N1B4_2850 0.67 +1.7 3,199,049 + Pf1N1B4_2850 0.78 -0.5 3,199,049 + Pf1N1B4_2850 0.78 -0.7 3,199,049 + Pf1N1B4_2850 0.78 +0.4 3,199,049 + Pf1N1B4_2850 0.78 +0.5 3,199,049 + Pf1N1B4_2850 0.78 +0.1 3,199,049 + Pf1N1B4_2850 0.78 -0.2 3,199,049 + Pf1N1B4_2850 0.78 -0.5 3,199,049 + Pf1N1B4_2850 0.78 -2.3 3,199,049 + Pf1N1B4_2850 0.78 +0.4 3,199,050 - Pf1N1B4_2850 0.78 -1.2 3,199,050 - Pf1N1B4_2850 0.78 -0.5 3,199,050 - Pf1N1B4_2850 0.78 -0.9 3,199,050 - Pf1N1B4_2850 0.78 -1.7 3,199,050 - Pf1N1B4_2850 0.78 +0.5 3,199,050 - Pf1N1B4_2850 0.78 +1.1 3,199,252 - +2.4 3,199,291 + +1.1 3,199,291 + -0.9 3,199,410 + -0.5 3,199,410 + +0.6 3,199,410 + +0.7 3,199,411 - +0.5 3,199,411 - -2.0 3,199,436 + Pf1N1B4_2851 0.29 +0.3 3,199,437 - Pf1N1B4_2851 0.30 -2.2 3,199,437 - Pf1N1B4_2851 0.30 -0.6 3,199,461 + Pf1N1B4_2851 0.47 -1.2 3,199,530 + +0.1 3,199,531 - +0.8 3,199,531 - +0.3 3,199,531 - -1.2 3,199,531 - +0.8 3,199,535 + +0.1 3,199,535 + -0.9 3,199,535 + +0.2 3,199,591 + +0.4 3,199,592 - -1.4 3,199,598 + +0.3 3,199,598 + +1.9 3,199,598 + -0.9 3,199,598 + +0.1 3,199,599 - +0.1 3,199,599 - +0.4 3,199,599 - +0.5 3,199,710 + Pf1N1B4_2852 0.16 -0.6 3,199,710 + Pf1N1B4_2852 0.16 +1.3 3,199,710 + Pf1N1B4_2852 0.16 +0.8 3,199,710 + Pf1N1B4_2852 0.16 -0.7 3,199,710 + Pf1N1B4_2852 0.16 -1.5 3,199,710 + -0.1 3,199,710 + Pf1N1B4_2852 0.16 +0.4 3,199,710 + Pf1N1B4_2852 0.16 -0.5 3,199,710 + Pf1N1B4_2852 0.16 +0.6 3,199,710 + Pf1N1B4_2852 0.16 +1.3 3,199,711 - Pf1N1B4_2852 0.16 -0.2 3,199,711 - Pf1N1B4_2852 0.16 +0.0 3,199,711 - Pf1N1B4_2852 0.16 -0.5 3,199,711 - Pf1N1B4_2852 0.16 +1.1 3,199,711 - Pf1N1B4_2852 0.16 +0.1 3,199,711 - Pf1N1B4_2852 0.16 +0.3 3,199,711 - Pf1N1B4_2852 0.16 +0.8 3,199,711 - Pf1N1B4_2852 0.16 +0.2 3,199,711 - Pf1N1B4_2852 0.16 +1.1 3,199,711 - Pf1N1B4_2852 0.16 -1.0 3,199,752 + Pf1N1B4_2852 0.27 +0.1 3,199,857 + Pf1N1B4_2852 0.55 -0.0 3,199,857 + Pf1N1B4_2852 0.55 +0.7 3,199,890 + Pf1N1B4_2852 0.63 -1.9 3,199,890 + Pf1N1B4_2852 0.63 -0.5 3,199,890 + Pf1N1B4_2852 0.63 +1.1 3,199,890 + Pf1N1B4_2852 0.63 -0.0 3,199,890 + Pf1N1B4_2852 0.63 -1.7 3,199,890 + Pf1N1B4_2852 0.63 -1.0 3,199,890 + Pf1N1B4_2852 0.63 +1.4 3,199,891 - Pf1N1B4_2852 0.64 +0.3 3,199,891 - Pf1N1B4_2852 0.64 -1.2 3,199,891 - Pf1N1B4_2852 0.64 -0.0 3,199,908 + Pf1N1B4_2852 0.68 -1.2 3,199,908 + Pf1N1B4_2852 0.68 +0.2 3,199,909 - Pf1N1B4_2852 0.68 +0.4 3,199,909 - Pf1N1B4_2852 0.68 +2.1 3,199,965 + Pf1N1B4_2852 0.83 +0.8 3,199,965 + Pf1N1B4_2852 0.83 -0.4 3,200,093 + -0.0 3,200,093 + -1.2 3,200,093 + -0.1 3,200,093 + +1.1 3,200,093 + -0.7 3,200,093 + -0.5 3,200,093 + -0.1 3,200,094 - -0.0 3,200,094 - -0.8 3,200,149 + -0.3 3,200,149 + +0.6 3,200,152 + +0.4 3,200,152 + -1.5 3,200,153 - +1.1 3,200,219 + -0.6 3,200,226 + +1.8 3,200,226 + -1.0 3,200,362 - Pf1N1B4_2853 0.16 +0.1 3,200,441 - Pf1N1B4_2853 0.25 +1.4 3,200,472 + Pf1N1B4_2853 0.29 -0.2
Or see this region's nucleotide sequence